BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	3,514,540	(G)_6→7	coding (316/2754 nt)	RPPX_15700 ←	chemotaxis protein CheY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	3,514,534	1	.	G	96.8%	885.3 / 19.4	253	coding (322/2754 nt)	RPPX_15700	chemotaxis protein CheY
Reads supporting (aligned to +/- strand): ref base . (6/2); new base G (122/123); total (128/125)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

CAGCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGC  >  CP009974/3514385‑3514678

cAGCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g                                                                                                                                                  >  2:604027/1‑151 (MQ=255)

 aGCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑g                                                                                                                                                <  1:3127086/151‑1 (MQ=255)

   cgcGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggg                                                                                                                                              <  1:1931571/149‑1 (MQ=255)

   cTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggg                                                                                                                                              >  1:1643621/1‑151 (MQ=255)

      gATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑gggggg                                                                                                                                           >  2:2352055/1‑150 (MQ=255)

       aTCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGAGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggggggc                                                                                                                                          <  2:2404191/151‑3 (MQ=255)

        tctcTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggggggca                                                                                                                                         >  2:269744/1‑148 (MQ=255)

        tctcTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggggggca                                                                                                                                         <  1:3414580/151‑4 (MQ=255)

        tctcTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑G‑GGGGGCAg                                                                                                                                         <  1:1218637/151‑1 (MQ=255)

         ctctACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggggggcag                                                                                                                                        >  1:390309/1‑147 (MQ=255)

          tctACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑g‑ggggggcagg                                                                                                                                       <  2:3683927/151‑6 (MQ=255)

           gtACCCGGCGCAGCAGGGGCTCGGCGGCGGCGGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGt                                                                                                                                       <  2:5207121/150‑1 (MQ=255)

           ctACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGt                                                                                                                                       >  2:4887184/1‑151 (MQ=255)

            tACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGGCGTTGCCACTGCGCTGCAGGGGCTGGGTGCCGGGCCCGCCC‑CT‑‑g‑ggggggccggtt                                                                                                                                     >  2:4167394/1‑144 (MQ=255)

              cccagcccagCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGa                                                                                                                                    <  2:1448616/151‑1 (MQ=255)

              cccagcccagCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGa                                                                                                                                    <  2:2165956/151‑1 (MQ=255)

              cccagcccagCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGa                                                                                                                                    <  1:4400452/151‑1 (MQ=255)

                cagcccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGc                                                                                                                                  <  2:1540046/151‑1 (MQ=255)

                  ggcgaggagGCGCGCGGCTTGGGCCGGCACGGCGTCGGTGGCCGGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCAt                                                                                                                                <  1:1320867/144‑1 (MQ=255)

                  gcccagcAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCag                                                                                                                                <  2:2862827/151‑2 (MQ=255)

                      agcagGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCtt                                                                                                                            >  1:5154297/1‑151 (MQ=255)

                       gcagGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGCTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTg                                                                                                                           >  2:4492436/1‑151 (MQ=255)

                         agGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCGCCG‑‑G‑GGGGGCAGGTTGAGCATGCTTggg                                                                                                                         >  2:1945415/1‑151 (MQ=255)

                          ggCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGc                                                                                                                        <  1:4243083/151‑1 (MQ=255)

                           gcgcTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGcc                                                                                                                       <  2:1327466/151‑1 (MQ=255)

                            cgcTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCg                                                                                                                      <  1:889666/151‑1 (MQ=255)

                             gcTCCGCTTCGGCTGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCgg                                                                                                                     <  2:1997549/151‑1 (MQ=255)

                              cTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGc                                                                                                                    >  2:3967234/1‑151 (MQ=255)

                              cTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGc                                                                                                                    <  2:1274692/151‑1 (MQ=255)

                                 ggCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTg                                                                                                                 >  1:3063995/1‑151 (MQ=255)

                                 ggCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTACCGGGTGGCGTCGTTGCCACTTCGCTGCAGGAGTTGGGTGCCGCTACCGCCG‑CT‑‑GAGGGGGCAGGCTGAGCATGCTTGGGCCGGCGTg                                                                                                                 >  2:1371046/1‑151 (MQ=255)

                                  gCTTCGGCCGGCACGGCGGCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGGGCATGCTTGGGCCGGCGTgg                                                                                                                <  2:550133/151‑1 (MQ=255)

                                  gCTGCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTgg                                                                                                                <  2:4046594/151‑1 (MQ=255)

                                     gcggccggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGcc                                                                                                             <  1:1129296/150‑1 (MQ=255)

                                         ccggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGgc                                                                                                         <  1:2998933/151‑1 (MQ=255)

                                          cggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGgcg                                                                                                        >  1:4049557/1‑151 (MQ=255)

                                          cggcACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGgcg                                                                                                        >  2:4552045/1‑151 (MQ=255)

                                              aCGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTg                                                                                                    >  2:2184142/1‑151 (MQ=255)

                                               cGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGc                                                                                                   <  1:3364464/151‑1 (MQ=255)

                                                   gTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCTTGCGGTc                                                                                               >  1:2613745/1‑151 (MQ=255)

                                                       gTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTGGG‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCgg                                                                                            >  1:539116/1‑151 (MQ=255)

                                                       gTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCggg                                                                                           >  1:4456036/1‑151 (MQ=255)

                                                         ggCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAg                                                                                                           >  2:87409/1‑133 (MQ=255)

                                                            cAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAg                                                                                      <  1:3538718/151‑1 (MQ=255)

                                                             aGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGa                                                                                     >  2:2740323/1‑151 (MQ=255)

                                                             aGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCCTGGCCAGa                                                                                     >  2:3226204/1‑151 (MQ=255)

                                                              ggTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGaa                                                                                    >  2:2277943/1‑151 (MQ=255)

                                                               gTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGaaa                                                                                   <  1:4918058/151‑1 (MQ=255)

                                                                tCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAAc                                                                                  <  1:4534582/151‑1 (MQ=255)

                                                                 cgcgGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACg                                                                                 >  2:508362/1‑151 (MQ=255)

                                                                   cgGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGcc                                                                               <  2:3703803/151‑1 (MQ=255)

                                                                   cgGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGcc                                                                               <  2:1082991/151‑1 (MQ=255)

                                                                   cgGTGGTGGCCGAACACGGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGcc                                                                               <  2:3449778/151‑1 (MQ=255)

                                                                     gtggtgGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGCACTGCCG‑CTG‑G‑GGGGGCAGGCTGAGCCTGCTTGCTCCGGCGTGTGTCAGACCTGTGCGGTCCGGTGCCAGATATGCCAc                                                                             >  2:2491085/1‑151 (MQ=255)

                                                                      tggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACt                                                                            <  1:817388/151‑1 (MQ=255)

                                                                      tggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACt                                                                            >  1:1612283/1‑151 (MQ=255)

                                                                       ggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTg                                                                           <  2:1155551/151‑1 (MQ=255)

                                                                       ggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTg                                                                           >  1:2400285/1‑151 (MQ=255)

                                                                       ggtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTg                                                                           >  1:4061074/1‑151 (MQ=255)

                                                                        gtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCGCTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgc                                                                          <  2:2091246/151‑1 (MQ=255)

                                                                        gtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgc                                                                          <  1:5105268/151‑1 (MQ=255)

                                                                        gtggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgc                                                                          >  1:108600/1‑151 (MQ=255)

                                                                         tggCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTgcg                                                                         <  1:4340398/151‑1 (MQ=255)

                                                                                    tGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGc                                                              <  1:2867056/151‑1 (MQ=255)

                                                                                     ggCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGcc                                                             <  2:1735692/151‑1 (MQ=255)

                                                                                      gcagcaGGTAGCGGGGGGCGTCGTCGCCCCTGGGCTCCAGGAGTGGGGTGCCGGCACCGCCGCCG‑‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCt                                                            <  2:4778155/151‑1 (MQ=255)

                                                                                       cATGAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTGGGGTGCCGGTACGGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTg                                                           <  2:3032518/151‑1 (MQ=255)

                                                                                           aGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGCCCGGGGCCACACGCGCCACTGCGCGCACCTCGGCCTGcccc                                                       >  2:2246444/1‑151 (MQ=255)

                                                                                            ggTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCa                                                      <  1:1764979/151‑1 (MQ=255)

                                                                                                gCGGCTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGAGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGt                                                  <  1:4199142/151‑1 (MQ=255)

                                                                                                 cGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGtt                                                 >  1:2807848/1‑151 (MQ=255)

                                                                                                   ggTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTtg                                               <  2:4228789/151‑1 (MQ=255)

                                                                                                    gTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGTCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTtgt                                              >  1:442349/1‑151 (MQ=255)

                                                                                                    gTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTtgt                                              <  2:1243717/151‑1 (MQ=255)

                                                                                                    gTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTtgt                                              >  1:452089/1‑151 (MQ=255)

                                                                                                       gCGTCGTTGCCGCTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCg                                           <  2:3834937/151‑1 (MQ=255)

                                                                                                         gtcgtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGc                                         >  1:2119291/1‑151 (MQ=255)

                                                                                                          tcgtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCa                                        >  1:947308/1‑151 (MQ=255)

                                                                                                            gtTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTGTGTGCGGCAAt                                      >  1:3859203/1‑151 (MQ=255)

                                                                                                             ttGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAAtt                                     >  1:4651066/1‑151 (MQ=255)

                                                                                                             ttGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAAtt                                     <  1:4972328/151‑1 (MQ=255)

                                                                                                                ccACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTGGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtctc                                  <  2:5036774/151‑1 (MQ=255)

                                                                                                                 cACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtctct                                 >  1:4797717/1‑151 (MQ=255)

                                                                                                                 cACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtctcc                                 >  1:532059/1‑150 (MQ=255)

                                                                                                                 cACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGCCCAGGCGCGGGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGGAATtctcc                                 >  2:2566553/1‑150 (MQ=255)

                                                                                                                  aCTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATtctctc                                <  1:2574441/151‑1 (MQ=255)

                                                                                                                   cTGCGCTGCAGGAGTTGGGGGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTcc                               <  2:4442720/151‑1 (MQ=255)

                                                                                                                    tGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCa                              <  1:1960282/151‑1 (MQ=255)

                                                                                                                    tGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACACCGGCCTGCTCCAGGGCTTGTGCGGCAATTCTCTccc                              >  2:2963944/1‑150 (MQ=255)

                                                                                                                      cgcTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGt                            <  1:3963359/151‑1 (MQ=255)

                                                                                                                      cgcTGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAACATGCTTGAGCCGGCGTGAGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGt                            <  2:729838/151‑1 (MQ=255)

                                                                                                                         tGCAGGAGTTGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCCATTCTCTCCAGTTgc                         >  2:3098360/1‑151 (MQ=255)

                                                                                                                            gggAGTTGGGGGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCa                      <  2:1821094/150‑1 (MQ=255)

                                                                                                                                 ttGGGTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGgcgc                 <  2:1015611/151‑1 (MQ=255)

                                                                                                                                   gggTGCCGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGa               >  2:1453105/1‑151 (MQ=255)

                                                                                                                                         cGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCggg         >  1:3315933/1‑151 (MQ=255)

                                                                                                                                         cGGTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCggg         >  1:372558/1‑151 (MQ=255)

                                                                                                                                          ggTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGc        >  2:4018926/1‑151 (MQ=255)

                                                                                                                                           gTACCGCCG‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGcc       <  1:1512964/151‑1 (MQ=255)

                                                                                                                                              ccgccg‑CTG‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATg    <  1:270906/151‑1 (MQ=255)

CAGCTCGATCTCTACCCAGCCCAGCAGGCGCTCGGCTTCGGCCGGCACGGCGTCGGTGGCCAGGTCGCGGTGGTGGCCGAACACTGGCATCAGGTAGCGGGTGGCGTCGTTGCCACTGCGCTGCAGGAGTTGGGTGCCGGTACCGCCG‑CT‑‑G‑GGGGGCAGGTTGAGCATGCTTGGGCCGGCGTGGGCCAGGCGCGTGCGGTCCGGGGCCAGAAACGCCACTGCGCGCACATCGGCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGC  >  CP009974/3514385‑3514678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`