Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,821,273 (G)6→7 coding (1032/1263 nt) RPPX_21445 → histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,821,2671.G90.0% 753.0 / 83.1 231coding (1026/1263 nt)RPPX_21445histidine kinase
Reads supporting (aligned to +/- strand):  ref base . (10/13);  new base G (100/108);  total (110/121)
Fisher's exact test for biased strand distribution p-value = 8.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

CAGTGCCAAGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAGCGTTCCA  >  CP009974/4821118‑4821412
                                                                                                                                                      |                                                                                                                                                  
cAGTGCCAAGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑g                                                                                                                                                   >  2:170081/1‑151 (MQ=255)
 aGTGCCAAGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑gg                                                                                                                                                  >  1:3776506/1‑151 (MQ=255)
     ccAAGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑gggggg                                                                                                                                              <  2:4746163/151‑1 (MQ=255)
      cAAGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑ggggggg                                                                                                                                             >  1:3503316/1‑150 (MQ=255)
       aaGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑gggggggc                                                                                                                                            >  1:759292/1‑149 (MQ=255)
       aaGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑GGGGGG‑Ca                                                                                                                                           >  2:654737/1‑151 (MQ=255)
        aGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑gggggggca                                                                                                                                           <  1:2582617/151‑4 (MQ=255)
          tgtCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑gggggggcaat                                                                                                                                         >  1:3658443/1‑146 (MQ=255)
           gtCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATg                                                                                                                                        >  1:1258295/1‑151 (MQ=255)
             ccTTGCTGGCTCAGGGCGCGTTTGTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGt                                                                                                                                      <  2:726759/151‑1 (MQ=255)
              cTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGtt                                                                                                                                     <  2:3624627/151‑1 (MQ=255)
              cTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGtt                                                                                                                                     >  1:2649222/1‑151 (MQ=255)
              cTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGtt                                                                                                                                     >  2:2448801/1‑151 (MQ=255)
                    ggCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACTAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGgt                                                                                                                               >  1:3344998/1‑151 (MQ=255)
                    ggCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAACGGTGGCAGgt                                                                                                                               >  2:1409065/1‑151 (MQ=255)
                     gCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGgtg                                                                                                                              <  1:2323402/151‑1 (MQ=255)
                     gCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGgtg                                                                                                                              <  1:4941961/151‑1 (MQ=255)
                      cTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGgtgt                                                                                                                             >  2:3572838/1‑151 (MQ=255)
                      cTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACCGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGgtgt                                                                                                                             <  2:4902272/151‑1 (MQ=255)
                       tCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGtgtg                                                                                                                            >  2:952461/1‑151 (MQ=255)
                       tCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGGGCGCTCCACCAAGACCGGCACGGCGGTATTGGTGAAGGCCCGACCCGCACCC‑GGGGGGCCAATGGTTGCAgcgctg                                                                                                                            >  2:5213317/1‑146 (MQ=255)
                        cAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGc                                                                                                                           >  1:4360766/1‑151 (MQ=255)
                           ggCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTc                                                                                                                        >  1:3133420/1‑151 (MQ=255)
                           ggCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTc                                                                                                                        <  1:3008833/151‑1 (MQ=255)
                           ggCGCGTTTCGCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTc                                                                                                                        <  2:2389021/151‑1 (MQ=255)
                             cgcgTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAg                                                                                                                      >  1:1622161/1‑151 (MQ=255)
                                ttttCTCATGCATGGCCATGAAGAGCTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGa                                                                                                                   <  2:990419/150‑1 (MQ=255)
                                 tttCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGAc                                                                                                                  <  2:1850768/151‑1 (MQ=255)
                                  ttCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGccc                                                                                                                 >  2:4318362/1‑151 (MQ=255)
                                   tctcATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCa                                                                                                                <  1:990917/151‑1 (MQ=255)
                                    ctcATGCAGGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCAt                                                                                                               <  1:3608293/151‑1 (MQ=255)
                                      catgcatgGCCATGAAGGACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATgg                                                                                                             <  2:3013990/151‑1 (MQ=255)
                                      catgcatgGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATgg                                                                                                             >  1:2038021/1‑151 (MQ=255)
                                      catgcatgGCCACGAAGAACTCCGGTACCGGGCCTTCGAGAACGTCGTCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATgg                                                                                                             <  2:1395449/151‑1 (MQ=255)
                                        tgcatgGCCAGGAAGAACGCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGcc                                                                                                           <  1:1090137/151‑1 (MQ=255)
                                          catgGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCa                                                                                                         <  1:3234910/151‑1 (MQ=255)
                                           atgGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAg                                                                                                        >  2:1194318/1‑151 (MQ=255)
                                            tgGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGc                                                                                                      >  1:2876029/1‑151 (MQ=255)
                                            gggCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAgg                                                                                                       <  2:2631029/150‑1 (MQ=255)
                                              gCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCg                                                                                                     <  1:3872677/151‑1 (MQ=255)
                                              gCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCg                                                                                                     >  2:1306254/1‑151 (MQ=255)
                                                 aTGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCACAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCg                                                                                                  <  2:174542/151‑1 (MQ=255)
                                                   gaagaaCTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGAc                                                                                                >  2:342973/1‑151 (MQ=255)
                                                   gaagaaCTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGGCTGCGGCAGCGCCCATGGCCCAGGCGTCGAc                                                                                                >  2:2910903/1‑151 (MQ=255)
                                                     agaaCTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGAccc                                                                                              <  1:5174891/151‑1 (MQ=255)
                                                     agaaCTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGAccc                                                                                              <  2:2135832/151‑1 (MQ=255)
                                                           ccTGTACCGGGCATTCGAGAACGTCATCCGCAATGCCGTGCGCCACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGgcgcg                                                                                        <  1:1804585/151‑1 (MQ=255)
                                                             tGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGcc                                                                                      >  2:2292680/1‑151 (MQ=255)
                                                              gTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCt                                                                                     >  2:2243379/1‑151 (MQ=255)
                                                              gTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCt                                                                                     >  1:2336908/1‑151 (MQ=255)
                                                               tACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTg                                                                                    >  1:4552713/1‑151 (MQ=255)
                                                                aCCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGc                                                                                   >  1:4731053/1‑151 (MQ=255)
                                                                 ccGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCa                                                                                  <  1:3296094/151‑1 (MQ=255)
                                                                 ccGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAg                                                                                 >  2:3293580/1‑151 (MQ=255)
                                                                   gggCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGc                                                                                <  2:529341/151‑1 (MQ=255)
                                                                   gggCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGc                                                                                >  2:5071060/1‑151 (MQ=255)
                                                                   gggCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCg                                                                               >  1:4755986/1‑151 (MQ=255)
                                                                    ggCCTTGGAGAACGTCATCCGCAATGCCGTGCGCTCCACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCg                                                                               <  2:4064750/151‑1 (MQ=255)
                                                                          cGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCtt                                                                         <  1:1085363/151‑1 (MQ=255)
                                                                              aaCGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGaa                                                                     <  1:3950177/151‑1 (MQ=255)
                                                                              aaCGGCATGCGCAATGCCGTGCGCTACACCAAGACCGGCAGGGCGGTGTTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGaa                                                                     <  2:4163996/151‑1 (MQ=255)
                                                                               aCGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAAc                                                                    <  1:3592715/151‑1 (MQ=255)
                                                                                 gTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCg                                                                  >  2:1730155/1‑151 (MQ=255)
                                                                                  tCATCCGCAATGCCGTGCGCTACACCAAGACCGGCATGGCGGTATTGGTGAACGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGt                                                                 <  2:4614998/151‑1 (MQ=255)
                                                                                     tCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCg                                                              <  1:3125224/151‑1 (MQ=255)
                                                                                      ccGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGa                                                             <  1:2221348/151‑1 (MQ=255)
                                                                                           aTGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTg                                                        >  1:4416827/1‑151 (MQ=255)
                                                                                                gTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCAcc                                                   >  1:341118/1‑151 (MQ=255)
                                                                                                gTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCAcc                                                   >  1:1032151/1‑151 (MQ=255)
                                                                                                 tGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCg                                                  <  1:417444/151‑1 (MQ=255)
                                                                                                  gcgcTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGa                                                 >  1:667847/1‑151 (MQ=255)
                                                                                                    gcTACACCAAGGCCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGAca                                               <  1:3829240/151‑1 (MQ=255)
                                                                                                         acCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACagcagc                                          <  1:342973/151‑1 (MQ=255)
                                                                                                         acCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACagcagc                                          <  1:4892709/151‑1 (MQ=255)
                                                                                                          ccAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCg                                         <  2:31877/151‑1 (MQ=255)
                                                                                                             aGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACg                                      >  2:27317/1‑151 (MQ=255)
                                                                                                             aGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACg                                      <  1:1749387/151‑1 (MQ=255)
                                                                                                               gccGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGc                                    <  2:5168741/150‑1 (MQ=255)
                                                                                                               aCCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGc                                    <  1:422400/151‑1 (MQ=255)
                                                                                                                ccGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCt                                   >  1:46774/1‑151 (MQ=255)
                                                                                                                  ggCACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCttt                                 <  2:2007578/151‑1 (MQ=255)
                                                                                                                   gCACGGCGGTATGGGTGGAGGCCGCACCGGCAGCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTg                                <  2:2090212/151‑1 (MQ=255)
                                                                                                                    cACGGCGGTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTgg                               >  1:1553333/1‑151 (MQ=255)
                                                                                                                       ggcggTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGccc                            >  2:2813592/1‑150 (MQ=255)
                                                                                                                         cggTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTCCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTgg                          >  1:3941935/1‑151 (MQ=255)
                                                                                                                          gggaTTGGTGAAGGCCGCGCCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGGCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTggg                         <  1:4620235/148‑1 (MQ=255)
                                                                                                                           gTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTgggg                        <  2:3904996/151‑1 (MQ=255)
                                                                                                                           gTATTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTgggg                        <  2:3958619/151‑1 (MQ=255)
                                                                                                                             aTTGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCt                      >  2:4505129/1‑151 (MQ=255)
                                                                                                                              ttGGTGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTg                     <  1:2971225/151‑1 (MQ=255)
                                                                                                                                  tGAAGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGa                 >  1:1208964/1‑151 (MQ=255)
                                                                                                                                    aaGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATc               >  1:3995705/1‑151 (MQ=255)
                                                                                                                                    aaGGCCGCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATc               <  2:849608/151‑1 (MQ=255)
                                                                                                                                      ggCCGCACCGGCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGAGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGcc            <  1:2813462/151‑1 (MQ=255)
                                                                                                                                          ggaCCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAg         <  2:4792017/149‑1 (MQ=255)
                                                                                                                                          gCACCGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAg         >  2:1534553/1‑151 (MQ=255)
                                                                                                                                             ccGGCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAGCGt      <  2:3802169/151‑1 (MQ=255)
                                                                                                                                               ggCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAGCGTTc    >  1:4272896/1‑151 (MQ=255)
                                                                                                                                               ggCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAGCGTTcc   <  1:2770820/151‑1 (MQ=255)
                                                                                                                                                gCACCAGGGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAGCGTTcc   >  2:1080619/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CAGTGCCAAGTGTCCTTGCTGGCTCAGGGCGCGTTTCTCATGCATGGCCATGAAGAACTCCTGTACCGGGCCTTCGAGAACGTCATCCGCAATGCCGTGCGCTACACCAAGACCGGCACGGCGGTATTGGTGAAGGCCGCACCGGCACCA‑GGGGGG‑CAATGGTTGCAGGTGTGCGTCAGCGACCATGGCCCAGGCGTCGACCCGGCGCGCCTGCAGCGTATCTTCGAACCGTTCGACCGTGGCACCGACAGCAGCGACGGCTTTGGCCTGGGGCTGGCGATCGCCCAGCGTTCCA  >  CP009974/4821118‑4821412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑