BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	738,452	(C)_6→7	coding (1308/1347 nt)	RPPX_03230 ←	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	738,446	1	.	C	98.9%	964.4 / 2.4	268	coding (1314/1347 nt)	RPPX_03230	histidine kinase
Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (137/128); total (138/130)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TTTCCGCCCGATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑CCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTGGTCGACGG  >  CP009974/738296‑738591

  tCCGCCCGATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑cc                                                                                                                                                 >  1:4910757/1‑151 (MQ=255)

   ccGCCCGATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑ccc                                                                                                                                                <  2:4982158/151‑1 (MQ=255)

     gCCCGATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTTCCGGCCCATTCGCGGATTGAACTGTGAACAGGCTGGCACAGGCTAGGAATCAATCCAGGGGGAATGTGATCCTGAACGCAGCG‑ccccc                                                                                                                                              >  2:2548451/1‑151 (MQ=255)

     gCCCGATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑ccccc                                                                                                                                              <  1:4808686/151‑1 (MQ=255)

         gATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATGCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGCACTGTGAACGGGCTGGCACAGGCTAGGAATCAATGCAGGTAGAAGGTGATCCTGAACGCAGCA‑cccccccag                                                                                                                                          >  2:4096132/1‑148 (MQ=255)

         gATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑cccccccag                                                                                                                                          >  1:1300833/1‑148 (MQ=255)

         gATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑cccccccag                                                                                                                                          <  1:4649937/151‑4 (MQ=255)

          aTGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑cccccccagc                                                                                                                                         <  2:4125709/151‑5 (MQ=255)

           tGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑cccccccagcg                                                                                                                                        >  2:3493355/1‑146 (MQ=255)

            ggCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAgcgg                                                                                                                                        >  1:4795180/1‑151 (MQ=255)

             gCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAgcggc                                                                                                                                       >  2:3833997/1‑151 (MQ=255)

              cGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAgcggcg                                                                                                                                      >  1:2770742/1‑151 (MQ=255)

              cGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAgcggcg                                                                                                                                      >  1:3979116/1‑151 (MQ=255)

                gAATTTGTACAAGGAGGAAGGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGaa                                                                                                                                    <  2:2920411/151‑1 (MQ=255)

                 aaTTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAAt                                                                                                                                   <  1:460806/151‑1 (MQ=255)

                  atgggTACAAGGAGGAATGGCGGAAATCGGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGGGGATTGAACTGTGGACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATc                                                                                                                                  <  2:4463061/147‑1 (MQ=255)

                   tttGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCg                                                                                                                                 >  1:1245264/1‑151 (MQ=255)

                      gTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCa                                                                                                                              <  1:1565950/151‑1 (MQ=255)

                       tACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCaa                                                                                                                             >  1:311340/1‑151 (MQ=255)

                           aggaggAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCt                                                                                                                         >  1:3591001/1‑151 (MQ=255)

                             gaggaATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑CCCCCCAGCGGCGAATCGCCAAGGCTCAg                                                                                                                      <  1:1440800/151‑1 (MQ=255)

                               ggaATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGc                                                                                                                     >  2:3992275/1‑151 (MQ=255)

                                gaATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCt                                                                                                                    >  2:2033206/1‑151 (MQ=255)

                                gaATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCt                                                                                                                    >  2:1795794/1‑151 (MQ=255)

                                  aTGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCATCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCg                                                                                                                  >  2:3256179/1‑151 (MQ=255)

                                   tGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCgg                                                                                                                 >  2:1559631/1‑151 (MQ=255)

                                      cGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGt                                                                                                              <  1:297855/151‑1 (MQ=255)

                                      cGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGt                                                                                                              >  1:1668468/1‑151 (MQ=255)

                                         aaaTCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCAt                                                                                                           >  1:1225274/1‑151 (MQ=255)

                                         aaaTCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGACCCTGAACGCAGCGCCCCCCCAGCGGCGACTCGCCAAGGCCCAGCTCGGCGGCAt                                                                                                           >  1:2049185/1‑151 (MQ=255)

                                               gCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTCTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGt                                                                                                     >  1:3933242/1‑151 (MQ=255)

                                                cccTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTc                                                                                                    <  1:469372/151‑1 (MQ=255)

                                                cccTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTc                                                                                                    <  1:977798/151‑1 (MQ=255)

                                                 ccTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTcg                                                                                                   <  1:1948606/151‑1 (MQ=255)

                                                   tttGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTcgac                                                                                                 <  2:1651822/151‑1 (MQ=255)

                                                   tttGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTcgac                                                                                                 <  1:2598698/151‑1 (MQ=255)

                                                        ggATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGatat                                                                                            <  1:1823269/151‑1 (MQ=255)

                                                          aTTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATcc                                                                                          >  2:4778391/1‑151 (MQ=255)

                                                          aTTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCCCGGCGCCATAGCTGTCGACTATTTcc                                                                                          >  1:1032664/1‑151 (MQ=255)

                                                          aTTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCCAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCGCAGCCCGGCGCCGTAGCGGGCGGCGACATcc                                                                                          >  2:1250221/1‑151 (MQ=255)

                                                             ggcTGTGCCGGCCCATTCGGGGATTGAACTGTGCACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTg                                                                                       <  2:4237678/149‑1 (MQ=255)

                                                                tgtgCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGCTATCCTTGAcc                                                                                    >  2:4841206/1‑151 (MQ=255)

                                                                 gtgCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCa                                                                                   <  1:140010/151‑1 (MQ=255)

                                                                     cGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGc                                                                               >  1:3401012/1‑151 (MQ=255)

                                                                     cGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGc                                                                               >  2:1408527/1‑151 (MQ=255)

                                                                      ggCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGcc                                                                              >  2:3375573/1‑151 (MQ=255)

                                                                        cccATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCaa                                                                            <  1:4045899/151‑1 (MQ=255)

                                                                        cccATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCaa                                                                            >  1:1142783/1‑151 (MQ=255)

                                                                          cATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGc                                                                          >  2:2653071/1‑151 (MQ=255)

                                                                           aTTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGcc                                                                         <  1:784544/151‑1 (MQ=255)

                                                                                cgGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGAtgc                                                                    <  2:2770757/151‑1 (MQ=255)

                                                                                cgGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGAtgc                                                                    <  1:3631342/151‑1 (MQ=255)

                                                                                   aTTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGAtgccct                                                                 >  1:3686693/1‑151 (MQ=255)

                                                                                    ttGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGAtgccctg                                                                >  1:1655821/1‑151 (MQ=255)

                                                                                        aCTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGcccc                                                            <  1:2364346/151‑1 (MQ=255)

                                                                                        aCTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGcccc                                                            <  2:2212155/151‑1 (MQ=255)

                                                                                        aCTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGcccc                                                            <  1:2899964/151‑1 (MQ=255)

                                                                                            tgAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGt                                                        >  1:918412/1‑151 (MQ=255)

                                                                                              aaCGGGCCGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgc                                                      <  2:4664940/151‑1 (MQ=255)

                                                                                               aCGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGCCGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgct                                                     >  2:4024108/1‑151 (MQ=255)

                                                                                               aCGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgct                                                     <  2:658169/151‑1 (MQ=255)

                                                                                                 gggCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgctgg                                                   >  1:2180468/1‑151 (MQ=255)

                                                                                                  ggCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgctggc                                                  <  1:5148116/151‑1 (MQ=255)

                                                                                                    cTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgctggctg                                                <  1:3543219/151‑1 (MQ=255)

                                                                                                     tGGCACACGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGACCGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCCGTCGACGATCTCCTCGACCACTGCCAAGCCGATGCCCTGCCCCCGGCgctgcctgc                                               >  2:3764489/1‑150 (MQ=255)

                                                                                                      ggCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTc                                              >  1:805876/1‑151 (MQ=255)

                                                                                                      ggCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTc                                              <  2:3023368/151‑1 (MQ=255)

                                                                                                      cgCACAGGCTAGGACTCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTc                                              <  2:2830338/150‑1 (MQ=255)

                                                                                                        cacaGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCa                                            <  2:3563755/151‑1 (MQ=255)

                                                                                                        cacaGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTCTCCa                                            <  2:4299738/151‑1 (MQ=255)

                                                                                                           aGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGcc                                         >  1:551019/1‑151 (MQ=255)

                                                                                                             gCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGc                                       <  1:1190273/151‑1 (MQ=255)

                                                                                                               tAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTgctggct                                                 >  1:5235932/1‑139 (MQ=255)

                                                                                                               tAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTc                                     >  1:3813888/1‑151 (MQ=255)

                                                                                                                 ggAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTccc                                   <  2:1943203/151‑1 (MQ=255)

                                                                                                                  gAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTcccc                                  <  2:5122807/151‑1 (MQ=255)

                                                                                                                    atcaatcCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCAc                                >  2:227298/1‑151 (MQ=255)

                                                                                                                     tcaatcCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCAcc                               >  2:4755557/1‑150 (MQ=255)

                                                                                                                         tcCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTcc                           <  1:1962906/151‑1 (MQ=255)

                                                                                                                         tcCAGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTcc                           <  2:1344509/151‑1 (MQ=255)

                                                                                                                          tcAGGTGGAAGGTGATCCTGCACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCa                          <  2:2475278/150‑1 (MQ=255)

                                                                                                                            aGGTGGAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGa                        >  1:188712/1‑151 (MQ=255)

                                                                                                                                 gAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCg                   >  2:56806/1‑151 (MQ=255)

                                                                                                                                 gAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCg                   >  1:4395015/1‑151 (MQ=255)

                                                                                                                                 gAAGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCg                   <  1:3903366/151‑1 (MQ=255)

                                                                                                                                  aaGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTTTCCACCCGCCCCCCACGCTCCACAAcgcgc                  >  2:4689281/1‑150 (MQ=255)

                                                                                                                                  aaGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGt                  <  1:595599/151‑1 (MQ=255)

                                                                                                                                  aaGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGATCCAGAATGCgc                  >  1:367082/1‑150 (MQ=255)

                                                                                                                                   aGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGtt                 >  2:3897578/1‑151 (MQ=255)

                                                                                                                                   aGGTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCCCCAGAATGCGtt                 >  1:4248522/1‑151 (MQ=255)

                                                                                                                                    ggTGATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTc                >  2:2999717/1‑151 (MQ=255)

                                                                                                                                       gATCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACg             >  1:285952/1‑151 (MQ=255)

                                                                                                                                         tCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCt           >  2:362405/1‑151 (MQ=255)

                                                                                                                                         tCCTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCGGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCt           <  2:1211608/151‑1 (MQ=255)

                                                                                                                                          ccTGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTg          <  1:421835/151‑1 (MQ=255)

                                                                                                                                            tGAACGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGCATGCGTTCACGCTGGt        >  2:3701125/1‑151 (MQ=255)

                                                                                                                                              aaCGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTGGTCg      >  2:835371/1‑151 (MQ=255)

                                                                                                                                               aCGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTGGTCGa     >  1:2275829/1‑151 (MQ=255)

                                                                                                                                               aCGCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTGGTCGa     <  2:2370616/151‑1 (MQ=255)

                                                                                                                                                 gCAGCGCCCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTGGTCGACg   <  1:3353839/151‑1 (MQ=255)

TTTCCGCCCGATGGCGGAATTTGTACAAGGAGGAATGGCGGAAATCCGCCCTTTGTGGATTGCCTGTGCCGGCCCATTCGCGGATTGAACTGTGAACGGGCTGGCACAGGCTAGGAATCAATCCAGGTGGAAGGTGATCCTGAACGCAGCG‑CCCCCCAGCGGCGAATCGCCAAGGCTCAGCTCGGCGTCATAGCTGTCGACGATATCCTTGACCACTGCCAAGCCGATGCCCTGCCCCGGGTGCTGGCTGTCCAGCCGCTCCCCACGCTCCAGAATGCGTTCACGCTGGTCGACGG  >  CP009974/738296‑738591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`