Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,105,2640GA75.0% ‑2.1 / 13.6 28A196A (GCC→GCT) RPPX_22650benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base A (13/8);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01
Rejected as consensus: E-value score below prediction cutoff.

TCTTGATCTCGTCACCCGCCTCGCGCTGCTTGCGCTGGTTCTGGGTGGCAGCGTAGTTCCAGTGCACGGAGCTTACGTGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTA  >  CP009974/5105125‑5105395
                                                                                                                                           |                                                                                                                                   
ttttGATTTCGTCGCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACgcag                                                                                                                          <  2:2052055/149‑1 (MQ=1)
ttttGATTTCGTCGCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACgcag                                                                                                                          >  1:435692/3‑151 (MQ=0)
ttttGATTTCGTCGCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACgcag                                                                                                                          <  1:4645983/149‑1 (MQ=0)
     atttcgtcgCCTGCCTCGCGGTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACtt                                                                                                                     <  2:1633285/148‑1 (MQ=1)
     atttcgtcgCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGGACtt                                                                                                                     >  1:4410420/4‑151 (MQ=1)
     atttcgtcgCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACtt                                                                                                                     >  2:4904446/4‑151 (MQ=1)
     atttcgtcgCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACtt                                                                                                                     >  2:269696/4‑151 (MQ=1)
     atttcgtcgCCTGCCTCGCGCTGCTTGCGCTGGTTCTGAGTGGCGGCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACtt                                                                                                                     >  1:3239041/4‑151 (MQ=0)
       ctcgcgaCCCGCCCCGCGCTGCGTGCGCCGGCTCTGGGTGGCAGCGTAGTTCCAGTGCACGGGGCTTACGGGGTCGCCGTCGGCGCCGTTTTCGGCGGTGAGCTGCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTcc                                                                                                                   <  2:3322828/145‑1 (MQ=1)
                                               gcggCGTAGTTCCAGTGCACGGAGCTAACATGATAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCt                                                                           >  1:139127/4‑151 (MQ=1)
                                                                   ggggCTTACGGGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGc                                                       <  2:3680425/148‑1 (MQ=1)
                                                                         aacatgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGAcc                                                 <  2:4147227/144‑1 (MQ=1)
                                                                            atgatAGCGGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                              <  2:667921/147‑1 (MQ=1)
                                                                            atgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                              >  2:13944/5‑151 (MQ=1)
                                                                            atgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                              <  1:2636810/147‑1 (MQ=1)
                                                                             tgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTg                                             >  2:1432336/4‑151 (MQ=1)
                                                                                   ccGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTca                                       >  1:5075057/1‑151 (MQ=0)
                                                                                   ccGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTca                                       >  1:4544586/1‑151 (MQ=0)
                                                                                               ttctCGGCGATCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCa                           >  2:2585471/4‑151 (MQ=1)
                                                                                                        gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                  >  2:1220759/4‑151 (MQ=1)
                                                                                                        gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                  >  1:3043802/4‑151 (MQ=1)
                                                                                                        gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                  >  1:3043435/4‑151 (MQ=1)
                                                                                                        gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                  >  1:150966/4‑151 (MQ=1)
                                                                                                                   gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc       <  1:884125/151‑1 (MQ=1)
                                                                                                                   gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc       <  1:3049541/151‑1 (MQ=1)
                                                                                                                    ttaccTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGcccc      <  1:3706044/148‑1 (MQ=1)
                                                                                                                    ttaccTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGcccc      <  1:1110405/148‑1 (MQ=1)
                                                                                                                        ctgcGTAGGTGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCAGAACAAAAAGCCCCGGTa  <  2:2022719/148‑1 (MQ=1)
                                                                                                                                           |                                                                                                                                   
TCTTGATCTCGTCACCCGCCTCGCGCTGCTTGCGCTGGTTCTGGGTGGCAGCGTAGTTCCAGTGCACGGAGCTTACGTGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTA  >  CP009974/5105125‑5105395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑