| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009974 | 2,738,417 | 0 | A | G | 76.0% | ‑3.6 / 11.2 | 25 | E210E (GAA→GAG) | RPPX_12165 | benzene 1,2‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (9/10); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
AATGCCGATGTGAAACCACTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGC > CP009974/2738271‑2738541 | aacgCCGATGTGAAGCCGCTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACg > 1:4766890/4‑151 (MQ=0) gacgccgcTGGTCGAGCACCTGGGCGAGTCGGCGAACATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGc < 1:4194278/144‑1 (MQ=0) aagccgcTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCt < 1:1639199/145‑1 (MQ=1) aagccgcTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCt < 1:3092175/145‑1 (MQ=1) gccgcTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTAc > 2:2087755/5‑151 (MQ=1) ccgcTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTAcc > 1:3999139/4‑151 (MQ=1) aGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGACCAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTc > 1:1682676/1‑151 (MQ=1) gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc > 2:4361186/4‑151 (MQ=1) gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc > 1:2476636/4‑151 (MQ=0) gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc < 1:3838177/148‑1 (MQ=1) gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc > 1:870881/4‑151 (MQ=0) gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc > 1:1466668/4‑151 (MQ=0) cTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACg > 1:4531903/1‑151 (MQ=1) tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg < 1:2224635/144‑1 (MQ=1) ggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGa > 1:4090766/7‑151 (MQ=1) agctaGCTCCTACGTCTACGAAGGTAACTGCAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc < 2:2485100/147‑2 (MQ=1) agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc < 1:3911754/147‑2 (MQ=1) agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc < 1:928391/147‑2 (MQ=1) agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc > 1:1887559/5‑150 (MQ=1) gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg > 1:4934264/4‑149 (MQ=1) gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg < 1:172489/148‑3 (MQ=1) gctaGCTCCTACAACTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGAAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg < 1:5227194/148‑3 (MQ=1) tcctACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGgct < 1:4932243/148‑2 (MQ=0) ggagggtaaCGGGAAGCTGACCGCCGAGAACGGGGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCCAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAg < 2:3519538/144‑1 (MQ=0) gaatgtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGc < 2:5068417/145‑1 (MQ=0) | AATGCCGATGTGAAACCACTGGTCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGC > CP009974/2738271‑2738541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |