Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 25,728 G→A intergenic (+153/‑418) RPPX_00140 → / → RPPX_00145 MarR family transcriptional regulator/(2Fe‑2S)‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00997425,7280GA98.2% 723.5 / ‑3.7 221intergenic (+153/‑418)RPPX_00140/RPPX_00145MarR family transcriptional regulator/(2Fe‑2S)‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (101/111);  total (106/115)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TGTTGTGACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTGGCCAATCTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACC  >  CP009974/25580‑25875
                                                                                                                                                    |                                                                                                                                                   
tgttgtGACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGc                                                                                                                                                   >  2:1042334/1‑151 (MQ=255)
  ttgtGACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCa                                                                                                                                                 <  2:2923519/151‑1 (MQ=255)
     tgACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAAtc                                                                                                                                              <  1:3828548/151‑1 (MQ=255)
     tgACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAAtc                                                                                                                                              <  2:233967/151‑1 (MQ=255)
     tgACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAAtc                                                                                                                                              >  1:2619931/1‑151 (MQ=255)
      gACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAAtct                                                                                                                                             >  2:2566818/1‑151 (MQ=255)
       aCTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAAtctc                                                                                                                                            <  1:2285086/151‑1 (MQ=255)
          gCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTcctc                                                                                                                                         >  2:2664489/1‑149 (MQ=255)
            cgcgGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTccc                                                                                                                                       <  2:246219/151‑1 (MQ=255)
              cgGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCccgc                                                                                                                                     >  2:2702177/1‑151 (MQ=255)
               ggTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGTCCTGCTAGCCAATCTCCTCccgcc                                                                                                                                    >  2:1926741/1‑151 (MQ=255)
                 tGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGt                                                                                                                                  <  2:2301985/151‑1 (MQ=255)
                  ggCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGACCTGCTAGCCACGCCCCGCCCGCCGtg                                                                                                                                 >  2:2839925/1‑150 (MQ=37)
                  ggCCGTCACAGAGACGCGGCGAGAGAGAACAAACACATCGCGGGCAAGATCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGtt                                                                                                                                 <  2:413230/151‑1 (MQ=25)
                     cGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCAc                                                                                                                              <  1:2898583/151‑1 (MQ=255)
                     cGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGCTCACCCGCGAATGGGCCGGTCCTGCCAGCCAATCTCCTCCCCCCGCTCAc                                                                                                                              >  2:1682173/1‑151 (MQ=37)
                      gTCACGGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCAcc                                                                                                                             <  1:1973030/151‑1 (MQ=255)
                       tCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCg                                                                                                                            <  1:4012699/151‑1 (MQ=255)
                         acaGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGtt                                                                                                                          <  2:1678380/151‑1 (MQ=255)
                         acaGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTCCACCGtt                                                                                                                          >  2:4146185/1‑151 (MQ=255)
                          caGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTg                                                                                                                         >  2:1160039/1‑151 (MQ=255)
                            gATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgcc                                                                                                                       <  1:2113653/151‑1 (MQ=255)
                             aTACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccg                                                                                                                      <  2:1458104/151‑1 (MQ=255)
                             aTACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGCTCACCGTTgccc                                                                                                                      >  2:4057932/1‑150 (MQ=255)
                               aCGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccgcc                                                                                                                    <  2:4908244/151‑1 (MQ=255)
                                cgcgGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccgccg                                                                                                                   >  2:4216204/1‑151 (MQ=255)
                                 gcgGCCTGAGAGATCAAGCAGATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccgccgc                                                                                                                  <  2:2434237/151‑1 (MQ=255)
                                 gcgGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccgccgc                                                                                                                  >  1:4917797/1‑151 (MQ=255)
                                  cgGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTcct                                                                                                                                          <  2:4038085/126‑2 (MQ=255)
                                   ggCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTGGCCAATCTCCACCCGCCGCTCACCGTTgccgccgccc                                                                                                                >  2:446087/1‑150 (MQ=255)
                                   ggCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCa                                                                                                                >  1:5103832/1‑151 (MQ=255)
                                     ccTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCAcc                                                                                                              <  1:4809168/151‑1 (MQ=255)
                                      cTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCg                                                                                                             >  1:1689599/1‑151 (MQ=255)
                                             aTCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCCTTCACCGTTGCCGCCGCCACCGTATTccc                                                                                                      >  2:2224342/1‑149 (MQ=255)
                                                aaGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCggg                                                                                                   >  1:2792491/1‑151 (MQ=255)
                                                 aGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGt                                                                                                  <  1:3365612/151‑1 (MQ=255)
                                                  gCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTa                                                                                                 <  1:4786026/151‑1 (MQ=255)
                                                    acaTCGCGGGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTaaa                                                                                               <  1:4232169/151‑1 (MQ=255)
                                                    acaTCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTaaa                                                                                               <  2:1295072/151‑1 (MQ=255)
                                                     caTCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAc                                                                                              <  1:4738600/151‑1 (MQ=255)
                                                      aTCGCGAGCAAGCTGGCTCACGTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAcc                                                                                             <  2:3645168/151‑1 (MQ=255)
                                                      aTCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCGCCCGCCGCTCACTGTTGCCGCCGCCGCCGTATTCGCGGGTCAAcc                                                                                             >  2:550629/1‑151 (MQ=18)
                                                       tCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAccc                                                                                            >  1:3610066/1‑151 (MQ=255)
                                                         gcgAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGATCACCGTTACCGCCGCCACCGTATTCGCGGGTAAACCCGc                                                                                          >  2:4354914/1‑151 (MQ=255)
                                                           gAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTc                                                                                        <  2:3817437/151‑1 (MQ=255)
                                                            aGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTcc                                                                                       <  1:982799/151‑1 (MQ=255)
                                                              cAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTa                                                                                     <  1:329892/151‑1 (MQ=255)
                                                              cAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTa                                                                                     <  1:2499018/151‑1 (MQ=255)
                                                                aGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTaca                                                                                   <  2:1747224/151‑1 (MQ=255)
                                                                 gctcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTacac                                                                                  <  1:2146938/151‑1 (MQ=255)
                                                                    gaatcACGTTGGACACGCATCGGTGGTTGGAAAGAGGCCGCCGTAGGAGCGGGTTCACCCGCGAGTGGGCTGGTCCTGCTAGCCAATGTCCTCACGCCGTTCCCCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACTCACg                                                                               <  2:515756/148‑1 (MQ=17)
                                                                      ctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCa                                                                             <  1:3712426/151‑1 (MQ=255)
                                                                        cacaTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATc                                                                           <  1:3465184/151‑1 (MQ=255)
                                                                        cacaTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATc                                                                           <  1:607705/151‑1 (MQ=255)
                                                                        cacaTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATc                                                                           >  2:1890121/1‑151 (MQ=255)
                                                                                acacGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTc                                                                   <  1:1160039/151‑1 (MQ=255)
                                                                                   cGCATCGCTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGc                                                                <  2:3223503/151‑1 (MQ=255)
                                                                                     cATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGcac                                                              <  2:5103832/151‑1 (MQ=255)
                                                                                      aTCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTCCGGcacg                                                             >  1:399774/1‑150 (MQ=255)
                                                                                      aTCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCAGCTCCTACACACGCATCGGGGGCTCGGccac                                                             >  2:3332839/1‑148 (MQ=255)
                                                                                       tCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAg                                                            <  1:1574970/151‑1 (MQ=255)
                                                                                       tCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAg                                                            <  1:1435282/151‑1 (MQ=255)
                                                                                           tggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCcg                                                        >  1:3730941/1‑151 (MQ=255)
                                                                                              ttGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGTTCCGCCACCCGCcctcg                                                     >  2:4658753/1‑151 (MQ=14)
                                                                                                 gACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAg                                                  <  1:2252394/151‑1 (MQ=255)
                                                                                                     caGGCCGTCGTAGGAGCGGGTTCGCCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGGTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGc                                              <  2:4475437/151‑1 (MQ=255)
                                                                                                     caGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTCCGAGc                                              >  2:3003955/1‑151 (MQ=255)
                                                                                                     caGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGCCACAGGCCGTCCTAGgcgc                                              >  2:1998236/1‑151 (MQ=255)
                                                                                                     caGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTCCGCCCCAGGCcgtcgcacgcgc                                              >  2:766694/1‑144 (MQ=14)
                                                                                                         ccGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGCCACAGGCCGTCGTAGGAGCgggc                                          >  1:4905926/1‑150 (MQ=255)
                                                                                                           gtcgtAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTc                                        <  1:5026210/151‑1 (MQ=255)
                                                                                                             cgtAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGTTTCGGCACAGGCGTTCGCAGGAGCGGGGTCAc                                      >  2:2397214/1‑151 (MQ=14)
                                                                                                             cgtAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACCCGCCGTCGTAGGCGCGGGCTCAc                                      >  2:1505812/1‑151 (MQ=17)
                                                                                                               gaGGACCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAAGCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTAGTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCAccc                                    <  2:2740886/150‑1 (MQ=17)
                                                                                                                gggAGCGTGTGCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCcg                                   <  2:1689599/150‑1 (MQ=255)
                                                                                                                aGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCcg                                   >  2:4864210/1‑151 (MQ=255)
                                                                                                                 ggAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                  >  1:182669/1‑151 (MQ=255)
                                                                                                                 ggAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                  <  1:855286/151‑1 (MQ=255)
                                                                                                                     cGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGaaa                              <  1:701467/151‑1 (MQ=255)
                                                                                                                       gcttcggccGCAAATGGGCCGGTCCTGCTCGCCAATCTCCTCCCGCCCGTCACCGGTGCCGCCGCCCCCGTATTCGCGGGTCACCCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAgg                            <  2:2237645/144‑1 (MQ=17)
                                                                                                                          tCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGCTCACCCGCGAAAGggcc                         >  1:1601648/1‑151 (MQ=255)
                                                                                                                           gcgccGGGAATGGGCCGGTCCTGCTAGGCAATCGCCTCCCGCCGGTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGggccg                        <  1:3063423/148‑1 (MQ=17)
                                                                                                                            ccccGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGggccgg                       <  1:2839925/150‑1 (MQ=255)
                                                                                                                            aCCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGggccgg                       >  1:4159842/1‑151 (MQ=255)
                                                                                                                                gcgAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCct                   >  2:3893670/1‑151 (MQ=255)
                                                                                                                                 cggaTGGGCCGGTCCTGCTGGCCGATCTCCTCCCGCCGTGGACCGTGGCCGCCGCCACCGTATTCGGGGGTAAACCCGCTCGTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCctg                  <  2:5116735/148‑1 (MQ=17)
                                                                                                                                  gagtGGGCGGGTCCGGCGCGCCAATCTCCTCCCGCCGTTCGCCGTGGCCGCCGCCCCCGTAGTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCctgc                 <  2:4528409/148‑1 (MQ=17)
                                                                                                                                  gAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTCCGGCCCACGCCGTCGTAGGAGCGGGCTCCCCCGCGAACCGGCCGCCCctcc                 >  2:3516163/1‑149 (MQ=17)
                                                                                                                                  gAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGCTCGGCCCAGGCCGTCGCAGGCGCGGGTTCACCCGCGAAACGTCCGGCCccgc                 >  2:3107236/1‑151 (MQ=17)
                                                                                                                                  gAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCCGGGGTTGGACACACGCCGTCCTAGGCCCACGCCCACCCGCGAACGCGCCAGcccgtc                 >  2:2106202/1‑148 (MQ=14)
                                                                                                                                    acgggCGGGTCCTGCGAGCCGCTCTCCTCCCGCCGTTCACCGTGTCCGCCGGCACCGTATTCGCGGGTACCCCCGCTCCCACACACGCATGGGGGGTTCGGCACAGGCCGTCGGAGGAGCGGGTTCGCCCGCGAAAGGGCCGGCCctgctg               <  2:3292687/149‑1 (MQ=14)
                                                                                                                                    aTGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAg                                               >  1:3118290/1‑119 (MQ=255)
                                                                                                                                        gCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCa           <  1:1505345/151‑1 (MQ=255)
                                                                                                                                           ggTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTCCGCCACACGCCGTCGTAGGAGCGGGTTCCCCCGCCAAAGCGCCGCCCCTGCTGGCCAAtc        >  2:4770062/1‑151 (MQ=14)
                                                                                                                                             tCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAAtctc      <  1:3008674/151‑1 (MQ=255)
                                                                                                                                              cgggctgGCCAACCCCCTCCCGCCGTCCACCGTTGCCGCCGCCACCGTAGTCGCGGGTAACCCCGCGCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTcc     <  2:1582769/148‑1 (MQ=17)
                                                                                                                                              ccTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTcc     <  1:2430446/151‑1 (MQ=255)
                                                                                                                                              ccTGCTAGCCAATCGCCTCCCGCCGTTCTCCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTcc     <  1:1038099/151‑1 (MQ=255)
                                                                                                                                               ctgctaGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCCCACGCCGTCGTACGAGCGGGCTCACCCGCGAAAGCGCCGGCCCGGCTGGCCAtcctccc    >  2:1251942/1‑150 (MQ=14)
                                                                                                                                                 gctaGCCAATCTCCTCCCGCCGTTCACCGTTACCGCCGCCACCGTATACACGGGAAAACCCGCTCCTACACACGCATCGTAGATCCCGCACAGCCCCGCATAAGAAAGAAAACACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCAcc  >  2:4720639/1‑151 (MQ=9)
                                                                                                                                                    |                                                                                                                                                   
TGTTGTGACTGCCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTGGCCAATCTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACC  >  CP009974/25580‑25875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑