Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,069,253 T→C Q252R (CAG→CGG)  RPPX_09275 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,069,2530TC99.2% 917.7 / ‑4.9 261Q252R (CAG→CGG) RPPX_09275hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (127/132);  total (128/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 262 total aligned reads displayed.

AGATCGCCCAGTTCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCTGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGTGCTGCTGA  >  CP009974/2069106‑2069400
                                                                                                                                                   |                                                                                                                                                   
    cGCCCAGTTCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGacac                                                                                                                                              >  2:787858/1‑151 (MQ=255)
       ccAGTTCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTAc                                                                                                                                           >  2:1992160/1‑151 (MQ=255)
        cAGTTCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACa                                                                                                                                          <  1:2606325/151‑1 (MQ=255)
          gTTCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACaaa                                                                                                                                        >  1:3191601/1‑151 (MQ=255)
           ttCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACTCTACAAAc                                                                                                                                       <  2:5175166/151‑1 (MQ=255)
           ttCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAAc                                                                                                                                       <  1:4169417/151‑1 (MQ=255)
            tcctccCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACt                                                                                                                                      <  2:1673043/151‑1 (MQ=255)
              ctccCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTcc                                                                                                                                    >  1:4004973/1‑151 (MQ=255)
               tccCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTCCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGCCCGTGCCACTACAAACTCCa                                                                                                                                   >  2:2581798/1‑151 (MQ=255)
                 ccGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATg                                                                                                                                 <  1:4459828/151‑1 (MQ=255)
                 ccGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATg                                                                                                                                 <  2:1485814/151‑1 (MQ=255)
                  cGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGc                                                                                                                                <  2:3868135/151‑1 (MQ=255)
                       cATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCa                                                                                                                           <  1:1005125/151‑1 (MQ=255)
                         tCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAAc                                                                                                                         <  2:1221170/151‑1 (MQ=255)
                          cTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAAcc                                                                                                                        <  2:128812/151‑1 (MQ=255)
                            tCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCaa                                                                                                                      <  2:1147790/151‑1 (MQ=255)
                            tCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCaa                                                                                                                      <  2:1266756/151‑1 (MQ=255)
                                tctATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCa                                                                                                                  >  1:4041576/1‑151 (MQ=255)
                                 ttATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAg                                                                                                                 <  2:85871/150‑1 (MQ=255)
                                 ctATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAg                                                                                                                 <  2:1534248/151‑1 (MQ=255)
                                   aTCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGAc                                                                                                               <  1:988013/151‑1 (MQ=255)
                                    tCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACg                                                                                                              >  1:3413286/1‑151 (MQ=255)
                                     ccATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGc                                                                                                             <  1:1437140/151‑1 (MQ=255)
                                      cATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCt                                                                                                            >  1:251638/1‑151 (MQ=255)
                                        ttCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTc                                                                                                          <  2:4960560/151‑1 (MQ=255)
                                              gATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTc                                                                                                    >  2:2230783/1‑151 (MQ=255)
                                               aTAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCt                                                                                                   >  2:2504319/1‑151 (MQ=255)
                                                tAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCtt                                                                                                  <  1:3342844/151‑1 (MQ=255)
                                                 aaGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTAAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCttt                                                                                                 <  1:1783626/151‑1 (MQ=255)
                                                  aGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTg                                                                                                <  1:2635765/151‑1 (MQ=255)
                                                   ggATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGa                                                                                               <  1:744795/151‑1 (MQ=255)
                                                   ggATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGa                                                                                               >  2:4330156/1‑151 (MQ=255)
                                                    gatcatCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGAc                                                                                              <  2:3523941/151‑1 (MQ=255)
                                                     atgatCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACg                                                                                             >  1:614516/1‑151 (MQ=255)
                                                     atgatCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACg                                                                                             <  1:4600135/151‑1 (MQ=255)
                                                      tgatCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGa                                                                                            >  1:1276799/1‑151 (MQ=255)
                                                       gatCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGAt                                                                                           <  1:3678766/151‑1 (MQ=255)
                                                             ccGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATg                                                                                     <  2:2491170/151‑1 (MQ=255)
                                                              cGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATgg                                                                                    >  1:1686854/1‑151 (MQ=255)
                                                               ggCATCCGGCTAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGc                                                                                   >  1:4662623/1‑151 (MQ=255)
                                                               ggCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGc                                                                                   <  1:5155800/151‑1 (MQ=255)
                                                                    ccGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATg                                                                              <  1:2327520/151‑1 (MQ=255)
                                                                     cGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGa                                                                             >  2:2030384/1‑151 (MQ=255)
                                                                       gATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGaaa                                                                           >  1:1198073/1‑151 (MQ=255)
                                                                       gATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGaaa                                                                           <  1:2910140/151‑1 (MQ=255)
                                                                       gATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCAATGTCTTTGACGATTCGATGGCGGATGaaa                                                                           <  1:1499695/151‑1 (MQ=255)
                                                                        aTAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAAc                                                                          <  1:89969/151‑1 (MQ=255)
                                                                        aTAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAAc                                                                          <  1:3928379/151‑1 (MQ=255)
                                                                          gagagGTTCGAGCCGAGCCGGAGCGGAGTTGGTGACTTCCGGGGGGATGTGCGCGAGCTCACCTCCGCGAGGCCGTGACACTACAAACTGCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTc                                                                        <  2:4922305/150‑1 (MQ=255)
                                                                          aaGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTc                                                                        <  1:4330156/151‑1 (MQ=255)
                                                                           agagATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCa                                                                       >  1:5200051/1‑151 (MQ=255)
                                                                             agaTTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCAtt                                                                     >  2:726091/1‑151 (MQ=255)
                                                                             agaTTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCAtt                                                                     >  2:1573407/1‑151 (MQ=255)
                                                                              gaTTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCAttt                                                                    >  1:1310290/1‑151 (MQ=255)
                                                                              gaTTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCAttt                                                                    >  1:3817243/1‑151 (MQ=255)
                                                                              gaTTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCAttt                                                                    >  1:4296221/1‑151 (MQ=255)
                                                                                ttCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTg                                                                  >  2:3271704/1‑151 (MQ=255)
                                                                                     aCCAATGCGAATCGAATTTGATGACTTCCTTGTGAATGTACTCAAACTCAGCTCCGCGAGGCCGTGACACTACAAACTCCCTGCATTCAACCAATCCAGACGCTTCACTGCCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATc                                                             <  2:5032495/151‑1 (MQ=255)
                                                                                     aCCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATc                                                             <  2:2405574/151‑1 (MQ=255)
                                                                                     aCCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTAAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCCGAGGCGTCACTGTCTTTGACGATTCAGTGGCGGATGAACCTCATTTTGGGAtg                                                             >  2:4248474/1‑150 (MQ=255)
                                                                                       cAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCaa                                                           >  2:1835694/1‑151 (MQ=255)
                                                                                       cAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCaa                                                           <  2:1927319/151‑1 (MQ=255)
                                                                                        aaGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCaaa                                                          <  2:3003630/151‑1 (MQ=255)
                                                                                         aGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCaaaa                                                         <  1:2190757/151‑1 (MQ=255)
                                                                                             gaatcgaatTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCTGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGCCGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATg                                                     >  1:2929051/1‑151 (MQ=255)
                                                                                              aatcgaatTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATgc                                                    >  1:5232271/1‑151 (MQ=255)
                                                                                                tcgaatTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATgccg                                                  >  1:2097252/1‑151 (MQ=255)
                                                                                                tcgaatTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATgccg                                                  >  1:4516259/1‑151 (MQ=255)
                                                                                                tcgaatTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATgccg                                                  <  2:2691038/151‑1 (MQ=255)
                                                                                                 cgaatTTGATGACTTCCTTGTGAATGTGCTCATGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATgccgc                                                 <  2:2261709/151‑1 (MQ=255)
                                                                                                    atTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCtt                                              <  2:4003332/151‑1 (MQ=255)
                                                                                                     tttGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTc                                             <  2:5112291/151‑1 (MQ=255)
                                                                                                      ttGATGACTTCCTTGTGAATGTGGTCAAGCGCACCTCAGCGAGGCCGTTACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTCAGATCAAAATATGCCGCCTGCg                                            <  2:484297/151‑1 (MQ=255)
                                                                                                           gaCTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCa                                       <  2:1601505/151‑1 (MQ=255)
                                                                                                            agttCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAACCTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAg                                      <  2:3328339/149‑1 (MQ=255)
                                                                                                             cTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGACGCCTTCGAACCAgg                                     >  2:3141672/1‑151 (MQ=255)
                                                                                                               tCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCa                                   <  2:2051753/151‑1 (MQ=255)
                                                                                                                 cTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCAcc                                 <  2:581886/151‑1 (MQ=255)
                                                                                                                 cTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCAcc                                 >  1:3008924/1‑151 (MQ=255)
                                                                                                                   tgtgAATGTGCTCAAGCTGACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACctt                               <  2:76656/151‑1 (MQ=255)
                                                                                                                    gtgAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACcttc                              <  1:668791/151‑1 (MQ=255)
                                                                                                                     tgAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGCTTCGCTGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGACCCAGGCACcttct                             >  1:4235076/1‑151 (MQ=255)
                                                                                                                     tgAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACcttct                             >  2:3789176/1‑151 (MQ=255)
                                                                                                                      gAATGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACcttctt                            <  2:4337836/151‑1 (MQ=255)
                                                                                                                        aTGTGCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCg                          >  2:1074885/1‑151 (MQ=255)
                                                                                                                          gtgCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGAt                        <  1:4812138/151‑1 (MQ=255)
                                                                                                                           tgCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGAtt                       <  1:2703687/151‑1 (MQ=255)
                                                                                                                           tgCTCAAGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGAtt                       >  1:2819489/1‑151 (MQ=255)
                                                                                                                                aaGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGc                  >  2:3729005/1‑151 (MQ=255)
                                                                                                                                aaGCTCACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGc                  >  1:3832262/1‑151 (MQ=255)
                                                                                                                                     cACCTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGAcc             <  1:1555159/151‑1 (MQ=255)
                                                                                                                                       ccTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAg           >  1:4052501/1‑151 (MQ=255)
                                                                                                                                       ccTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAg           <  1:2069600/151‑1 (MQ=255)
                                                                                                                                        cTCCGCGAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGt          <  1:3898725/151‑1 (MQ=255)
                                                                                                                                           cgcgAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGtgct       <  1:4914622/151‑1 (MQ=255)
                                                                                                                                            gcgAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGtgctg      >  1:1933622/1‑151 (MQ=255)
                                                                                                                                              gAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGTgctgct    >  2:4137464/1‑151 (MQ=255)
                                                                                                                                              gAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGTgctgct    <  2:2181349/151‑1 (MQ=255)
                                                                                                                                              gAGGCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGTgctgct    >  1:5015180/1‑151 (MQ=255)
                                                                                                                                                ggCCGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCGTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGTGCTGCTGa  >  2:4569878/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGATCGCCCAGTTCCTCCCGGGCCATCTTCACTCTATCCATTCTGCGATAAGGATGATCAACCGGCATCCGGATAAGAGATTCGAACCAAGCCGAATCGAATTTGATGACTTCCTTGTGAATGTGCTCAAGCTCACCTCCGCGAGGCTGTGACACTACAAACTCCATGCTTTCAACCAAGCCAGACGCTTCACTGTCTTTGACGATTCGATGGCGGATGAAACTCATTTTGAGATCAAAATATGCCGCCTTCGAACCAGGCACCTTCTTCGATTCCAGCCGACCAGTGCTGCTGA  >  CP009974/2069106‑2069400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑