Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,341,088 A→G D394G (GAT→GGT)  RPPX_10490 → 2‑octaprenyl‑6‑methoxyphenyl hydroxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,341,0880AG99.6% 938.4 / ‑5.3 268D394G (GAT→GGT) RPPX_104902‑octaprenyl‑6‑methoxyphenyl hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (127/139);  total (128/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 268 total aligned reads displayed.

GCTTCTCCGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGATCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTGGCG  >  CP009974/2340941‑2341235
                                                                                                                                                   |                                                                                                                                                   
gCTTCTCCGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTcc                                                                                                                                                  >  1:4608409/1‑151 (MQ=255)
gCTTCTCCGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTcc                                                                                                                                                  >  2:1139708/1‑151 (MQ=255)
gCTTCTCCGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGACGGGCCTGGGCACCCGCCCTGGTcc                                                                                                                                                  >  2:4135413/1‑151 (MQ=255)
  ttCTCCGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCcgc                                                                                                                                                <  1:3323582/151‑1 (MQ=255)
    ctcCGACGAGGCCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGGCGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCgg                                                                                                                                              <  2:2319240/151‑1 (MQ=255)
       cGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGc                                                                                                                                           >  2:5236136/1‑151 (MQ=255)
          ccAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAg                                                                                                                                        <  1:1002418/151‑1 (MQ=255)
              gTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCGTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCAt                                                                                                                                    <  2:4037687/151‑1 (MQ=255)
                cACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCCCCCGCCCTGGTCCGCGGGGCCAGGCATGa                                                                                                                                  >  2:184596/1‑151 (MQ=255)
                cACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGa                                                                                                                                  >  1:404653/1‑151 (MQ=255)
                   cggCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCa                                                                                                                               <  1:4950168/149‑1 (MQ=255)
                      ccTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAAcc                                                                                                                            >  1:3130674/1‑151 (MQ=255)
                       ctctTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAAccc                                                                                                                           <  2:2380057/151‑1 (MQ=255)
                        tctTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCcg                                                                                                                          <  1:274464/151‑1 (MQ=255)
                          ttCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCcgcg                                                                                                                        <  1:3612975/151‑1 (MQ=255)
                          ttCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCcgcg                                                                                                                        <  1:1461570/151‑1 (MQ=255)
                           tCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGt                                                                                                                       <  2:3004740/151‑1 (MQ=255)
                            cGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTa                                                                                                                      >  2:3383595/1‑151 (MQ=255)
                              gcagcaATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTaaa                                                                                                                    <  1:1250746/151‑1 (MQ=255)
                               cagcaATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAAc                                                                                                                   >  2:517417/1‑151 (MQ=255)
                                agcaATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACt                                                                                                                  >  2:4759020/1‑151 (MQ=255)
                                agcaATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACt                                                                                                                  >  2:3956235/1‑151 (MQ=255)
                                agcaATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACt                                                                                                                  <  1:936650/151‑1 (MQ=255)
                                  caATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTgg                                                                                                                >  1:1672791/1‑151 (MQ=255)
                                  caATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTgg                                                                                                                <  2:2164377/151‑1 (MQ=255)
                                  caATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTgg                                                                                                                >  1:1735480/1‑151 (MQ=255)
                                         ccGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCg                                                                                                         >  1:2315647/1‑151 (MQ=255)
                                          cGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCgg                                                                                                        <  1:708108/151‑1 (MQ=255)
                                          cGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCgg                                                                                                        <  1:1047394/151‑1 (MQ=255)
                                           gTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCcgc                                                                                                                         >  2:2760010/1‑133 (MQ=255)
                                           gTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCAGGAGCAACCCGCGTAAACTGGCGCGTCggg                                                                                                       >  2:5112436/1‑151 (MQ=255)
                                                tGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGgcgcgc                                                                                                  <  1:255038/151‑1 (MQ=255)
                                                 gACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCa                                                                                                 >  1:586255/1‑151 (MQ=255)
                                                  gcGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCAt                                                                                                <  2:393343/150‑1 (MQ=255)
                                                  aCGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCAt                                                                                                >  1:834662/1‑151 (MQ=255)
                                                    ggCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTCCGCACGCCAGGCGATGAGCCCGGGCACCCCCCCTCGCCCGCAAGGCCGGGCATGCGCAACCCACTCAAACCTGCCCGTCCGCCGCGCAtgc                                                                                              >  2:2967577/1‑151 (MQ=255)
                                                      gcGGGCGCAAGCTTGGCCTGCTCGGGGTCGACGTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCAtgctg                                                                                            <  1:2033416/150‑1 (MQ=255)
                                                      ccGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCAtgctg                                                                                            <  2:1929330/151‑1 (MQ=255)
                                                       cGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCAtgctgc                                                                                           <  1:3969337/151‑1 (MQ=255)
                                                        gggCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTgcg                                                                                          >  2:839819/1‑151 (MQ=255)
                                                        gggCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTgcg                                                                                          >  1:127965/1‑151 (MQ=255)
                                                        gggCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTgcg                                                                                          >  1:2675446/1‑151 (MQ=255)
                                                        gggCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTgcg                                                                                          >  1:4114239/1‑151 (MQ=255)
                                                           cgcAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTg                                                                                       <  1:294900/151‑1 (MQ=255)
                                                              aaCCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGAt                                                                                    >  1:4379378/1‑151 (MQ=255)
                                                              aaCCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGAt                                                                                    <  1:954589/151‑1 (MQ=255)
                                                               aCCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATg                                                                                   <  1:1148900/151‑1 (MQ=255)
                                                                 ctgggCCTGCGCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCt                                                                                 <  2:1487730/148‑1 (MQ=255)
                                                                 cTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCt                                                                                 >  1:4647612/1‑151 (MQ=255)
                                                                  ttGGCCGGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTg                                                                                <  2:2671441/151‑1 (MQ=255)
                                                                   tGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGa                                                                               <  2:4590448/151‑1 (MQ=255)
                                                                      ccTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAAcc                                                                            <  2:3421285/151‑1 (MQ=255)
                                                                          cTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTa                                                                        <  2:470819/151‑1 (MQ=255)
                                                                            cGGGCTCGACGTGTTGCCACCGGCAAACAGCTGGTTCGCACGCCAGGGGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAGCTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTAcc                                                                      <  2:1498360/151‑1 (MQ=255)
                                                                                cTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCAcc                                                                  <  1:4756769/151‑1 (MQ=255)
                                                                                  cGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGt                                                                >  2:69599/1‑151 (MQ=255)
                                                                                   gACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTa                                                               <  1:2553490/151‑1 (MQ=255)
                                                                                     ccTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTAcc                                                             <  1:5144617/151‑1 (MQ=255)
                                                                                       tGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTg                                                           <  1:1246291/151‑1 (MQ=255)
                                                                                        gTTGGCACCGGCAAAAAGGTGGTGCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTgg                                                          <  2:4845775/151‑1 (MQ=255)
                                                                                           gCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTg                                                       <  1:4779221/151‑1 (MQ=255)
                                                                                             cACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGcc                                                     <  1:4895048/151‑1 (MQ=255)
                                                                                               ccGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCgg                                                   <  1:3275515/151‑1 (MQ=255)
                                                                                                 ggCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTa                                                 >  1:2343974/1‑151 (MQ=255)
                                                                                                    aaaaaGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATcc                                              <  2:447322/151‑1 (MQ=255)
                                                                                                    aaaaaGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATcc                                              <  2:1759584/151‑1 (MQ=255)
                                                                                                    aaaaaGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATcc                                              >  2:1801563/1‑151 (MQ=255)
                                                                                                    aaaaaGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATcc                                              <  2:2625925/151‑1 (MQ=255)
                                                                                                     aaaaGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCg                                             >  1:1958365/1‑151 (MQ=255)
                                                                                                      aaaGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCgt                                            >  2:624483/1‑151 (MQ=255)
                                                                                                        aGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCgtgt                                          <  2:3722138/151‑1 (MQ=255)
                                                                                                         gCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGtgtg                                         >  1:390/1‑151 (MQ=255)
                                                                                                          cTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTgc                                        <  1:1895625/151‑1 (MQ=255)
                                                                                                          cTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTgc                                        >  1:3488933/1‑151 (MQ=255)
                                                                                                             gtgcGCACGCCGGGCGATGGGCCTGGGCACCCGCGCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTgcagc                                     <  2:1278606/148‑1 (MQ=255)
                                                                                                             gTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTTTACCCACCGTACCTGGGTGCCGGCATCCGTGTgcagc                                     >  1:4999312/1‑151 (MQ=255)
                                                                                                             gTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTgcagc                                     >  1:1524624/1‑151 (MQ=255)
                                                                                                                cGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGcaca                                  >  2:3542101/1‑151 (MQ=255)
                                                                                                                  cACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATc                                <  2:505180/151‑1 (MQ=255)
                                                                                                                  cACGCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATc                                >  1:2823643/1‑151 (MQ=255)
                                                                                                                     gCCAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGc                             <  1:2908155/151‑1 (MQ=255)
                                                                                                                       cAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGccc                           >  2:4638248/1‑151 (MQ=255)
                                                                                                                       cAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGccc                           <  2:2958509/151‑1 (MQ=255)
                                                                                                                       cAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGccc                           <  1:74168/151‑1 (MQ=255)
                                                                                                                       cAGGCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCATAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGccc                           <  1:3645771/151‑1 (MQ=255)
                                                                                                                          gCGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGa                        <  1:1801533/151‑1 (MQ=255)
                                                                                                                           cGATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGAc                       >  2:1564248/1‑151 (MQ=255)
                                                                                                                            gATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGAcc                      >  2:2877878/1‑151 (MQ=255)
                                                                                                                            gATGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGAcc                      >  1:4172903/1‑151 (MQ=255)
                                                                                                                             aTGGGCCTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCt                     <  2:1214410/151‑1 (MQ=255)
                                                                                                                                   cTGGGCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAg               >  1:3098695/1‑151 (MQ=255)
                                                                                                                                       gCACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTc           >  1:2474713/1‑151 (MQ=255)
                                                                                                                                        cACCCGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCa          >  1:3800924/1‑151 (MQ=255)
                                                                                                                                          gccGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAg        <  1:4413566/150‑1 (MQ=255)
                                                                                                                                           ccGCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCCGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGCACCTGCGTAGTGTCAAgc       >  2:3081956/1‑150 (MQ=255)
                                                                                                                                             gCCCTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTg     <  1:2145333/151‑1 (MQ=255)
                                                                                                                                              cccTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTgg    <  2:4310889/151‑1 (MQ=255)
                                                                                                                                               ccTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTGGc   <  2:2437147/151‑1 (MQ=255)
                                                                                                                                               ccTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTGGc   <  1:3384953/151‑1 (MQ=255)
                                                                                                                                                cTGGTCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTGGCg  >  2:317159/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTTCTCCGACCAGGTCACCCGCCTCTTCGGCAGCAATCAGCCGTTGCTGACGGCCGGGCGCAACCTTGGCCTGCTCGGGCTCGACCTGTTGCCACCGGCAAAAAGCTGGTTCGCACGCCAGGCGATGGGCCTGGGCACCCGCCCTGATCCGCGGGGCCAGGCATGAGCAACCCGCGTAAACTGGCGCGTCGGGCGCGCATGCTGCGCTGGATGCTGAACCTCTACCCACCGTACCTGGGTGCCGGTATCCGTGTGCAGCACATCAGCCCGGACCTGCGTAGTGTCAAGGTGGCG  >  CP009974/2340941‑2341235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑