Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,393,876 C→T G294D (GGT→GAT)  RPPX_10725 ← 3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,393,8760CT99.2% 945.2 / ‑3.4 253G294D (GGT→GAT) RPPX_107253‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (124/126);  total (126/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 257 total aligned reads displayed.

GTGCAGCAGACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCACCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAGGTGGTCCAGCT  >  CP009974/2393728‑2394023
                                                                                                                                                    |                                                                                                                                                   
 tgCAGCAGACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCaa                                                                                                                                                  <  1:3587232/151‑1 (MQ=255)
 tgCAGCAGACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCaa                                                                                                                                                  >  2:1304434/1‑151 (MQ=255)
  gcagcaGACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAAt                                                                                                                                                 <  2:1182808/151‑1 (MQ=255)
   cagcagACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAAtg                                                                                                                                                >  1:335741/1‑151 (MQ=255)
   cagcagACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAAtg                                                                                                                                                >  2:5234412/1‑151 (MQ=255)
   cagcagACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAAtg                                                                                                                                                >  1:4258163/1‑151 (MQ=255)
     gcagACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAAtgtg                                                                                                                                              <  1:4894937/151‑1 (MQ=255)
       agACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTgcg                                                                                                                                            >  2:2934080/1‑151 (MQ=255)
         ccGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTgcggc                                                                                                                                          <  1:5148177/150‑1 (MQ=255)
         aCGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTgcggc                                                                                                                                          <  1:2686104/151‑1 (MQ=255)
          cGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATCGgcggcg                                                                                                                                         >  2:3216204/1‑151 (MQ=255)
           ggTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGt                                                                                                                                        >  1:665866/1‑151 (MQ=255)
             tgtTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCa                                                                                                                                      <  2:177080/151‑1 (MQ=255)
               tttGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAgg                                                                                                                                    >  1:1264047/1‑151 (MQ=255)
               tttGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAgg                                                                                                                                    >  1:2880481/1‑151 (MQ=255)
                 tGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGAt                                                                                                                                  <  2:1943597/151‑1 (MQ=255)
                  gCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATc                                                                                                                                 >  2:692081/1‑151 (MQ=255)
                    cTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTGAGGATCAc                                                                                                                               >  1:4158141/1‑151 (MQ=255)
                    cTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCAc                                                                                                                               <  2:4171307/151‑1 (MQ=255)
                    cTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCAc                                                                                                                               <  1:23016/151‑1 (MQ=255)
                    cTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCAc                                                                                                                               >  1:195635/1‑151 (MQ=255)
                       ggTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGtt                                                                                                                            >  1:2622992/1‑151 (MQ=255)
                       ggTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGtt                                                                                                                            <  1:553485/151‑1 (MQ=255)
                        gtgtGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTGGGCGGTGGAACAATCAATGTGCGGCGTCAGGATCACGTTg                                                                                                                           >  1:1045883/1‑151 (MQ=255)
                          gtgCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTc                                                                                                                         >  1:4687891/1‑151 (MQ=255)
                          gtgCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTc                                                                                                                         >  2:125244/1‑151 (MQ=255)
                          gtgCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTc                                                                                                                         <  1:4242176/151‑1 (MQ=255)
                          gtgCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGCGCTTCGGCGCTGGACCCATCAATGTCCGGCGTCAGGATCACGTTgcc                                                                                                                         >  2:2787119/1‑149 (MQ=255)
                           tgCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCg                                                                                                                        <  1:714058/151‑1 (MQ=255)
                              tGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAgg                                                                                                                     <  1:829091/151‑1 (MQ=255)
                               ggCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGc                                                                                                                    >  2:4230120/1‑151 (MQ=255)
                               ggCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGc                                                                                                                    >  2:1448367/1‑151 (MQ=255)
                                   gggCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCcgcg                                                                                                                >  1:3711759/1‑151 (MQ=255)
                                   gggCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCcgcg                                                                                                                <  1:3534039/151‑1 (MQ=255)
                                     gCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCa                                                                                                              >  2:3723359/1‑151 (MQ=255)
                                            ccGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCAGCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCt                                                                                                       >  2:3239170/1‑151 (MQ=255)
                                             cGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTCCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCACCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCtt                                                                                                      >  1:3809165/1‑151 (MQ=255)
                                              gggAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCttt                                                                                                     <  1:3845123/151‑1 (MQ=255)
                                                  aGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGaa                                                                                                 >  2:1081140/1‑151 (MQ=255)
                                                  aGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGaa                                                                                                 <  1:1316925/151‑1 (MQ=255)
                                                   gTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAAc                                                                                                >  1:4838998/1‑151 (MQ=255)
                                                     tGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAActc                                                                                              >  1:905580/1‑151 (MQ=255)
                                                      gACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAActct                                                                                             <  1:5048855/151‑1 (MQ=255)
                                                         cGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTTGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGc                                                                                                            <  2:3861969/133‑1 (MQ=255)
                                                         cGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTtcg                                                                                          >  2:235762/1‑151 (MQ=255)
                                                         cGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTtcg                                                                                          <  2:5100845/151‑1 (MQ=255)
                                                           aGGACACCGAAGTGCCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTtcgtc                                                                                        <  2:4384003/151‑1 (MQ=255)
                                                             gACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTcgtcgt                                                                                      >  1:992492/1‑151 (MQ=255)
                                                              acacCGAAGTACCGGTGTCGCTGTACTTGGCCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGtt                                                                                     <  2:2238969/151‑1 (MQ=255)
                                                                acCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTgg                                                                                   <  1:2210116/151‑1 (MQ=255)
                                                                acCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTgg                                                                                   <  1:1475568/151‑1 (MQ=255)
                                                                 ccGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGa                                                                                  >  1:4050285/1‑151 (MQ=255)
                                                                  cGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAg                                                                                 >  1:2333575/1‑151 (MQ=255)
                                                                    aaGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCg                                                                               >  2:940666/1‑151 (MQ=255)
                                                                     aGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGt                                                                              <  1:3446292/151‑1 (MQ=255)
                                                                      gTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTg                                                                             <  2:3508216/151‑1 (MQ=255)
                                                                      gTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTg                                                                             <  1:3017581/151‑1 (MQ=255)
                                                                      gTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTg                                                                             <  1:2455769/151‑1 (MQ=255)
                                                                        aCCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCATGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGc                                                                           <  1:2759563/151‑1 (MQ=255)
                                                                           gTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCg                                                                        >  2:4591991/1‑151 (MQ=255)
                                                                           gTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCg                                                                        <  1:180030/151‑1 (MQ=255)
                                                                            ttGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGa                                                                       <  1:2931749/151‑1 (MQ=255)
                                                                            ttGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGa                                                                       >  1:1560869/1‑151 (MQ=255)
                                                                             tGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGAc                                                                      <  1:1194559/151‑1 (MQ=255)
                                                                              gTCGCTGTACTTGGCCAGTTTCTCGGCCAGTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGAcc                                                                     <  2:3278260/151‑1 (MQ=255)
                                                                              gTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGAcc                                                                     >  1:260297/1‑151 (MQ=255)
                                                                               tCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCg                                                                    >  1:449686/1‑151 (MQ=255)
                                                                                   tGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTTGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGaa                                                                >  1:432848/1‑151 (MQ=255)
                                                                                     tACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAaca                                                              <  1:3343393/151‑1 (MQ=255)
                                                                                       cTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACg                                                            >  2:4569605/1‑151 (MQ=255)
                                                                                         tGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTc                                                          <  2:2622992/151‑1 (MQ=255)
                                                                                          gACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCg                                                         >  1:4962790/1‑151 (MQ=255)
                                                                                             gaGTTTGTCGGTCACTTCCAGGCCGATGGTGGCCTGGGCTTCGGCGGTGGAACCATCACTGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTCCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATg                                                      <  2:3106443/150‑1 (MQ=255)
                                                                                              aGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATgg                                                     >  1:3489020/1‑151 (MQ=255)
                                                                                              aGTTTCGCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATgg                                                     <  2:4115242/151‑1 (MQ=255)
                                                                                                tttCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATggcg                                                   >  1:736431/1‑151 (MQ=255)
                                                                                                  tctcGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATggcggc                                                 >  2:3623861/1‑151 (MQ=255)
                                                                                                  tctcGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATggcggc                                                 <  2:2148013/151‑1 (MQ=255)
                                                                                                     cGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGcc                                              >  1:2471855/1‑151 (MQ=255)
                                                                                                     cGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGcc                                              <  1:4975310/151‑1 (MQ=255)
                                                                                                        ccACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGAt                                           >  2:1546247/1‑151 (MQ=255)
                                                                                                         cACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATc                                          >  2:1152289/1‑151 (MQ=255)
                                                                                                          aCTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCa                                         >  1:4532730/1‑151 (MQ=255)
                                                                                                               cAGGCCGATGTGGGCCTGGGGTTCGGCGGTGGCACCATCAATGTGCGGCGTGAGGAGCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCGTCGTCGTGGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGttc                                    <  2:3499535/151‑3 (MQ=255)
                                                                                                                aGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTGCGAACTCTTCGGCGGTGGAGCGTGGCTCGAGCCGGATCCCGTCGAGGGCAGCGCCGCTCAGgcgcc                                   >  2:4469628/1‑150 (MQ=255)
                                                                                                                 ggCCGGTGTTGGCCTGGGCCTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCtt                                  <  2:4814873/151‑1 (MQ=255)
                                                                                                                 ggCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCtt                                  <  2:3842992/151‑1 (MQ=255)
                                                                                                                            gCCTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATgg                       >  1:2259109/1‑151 (MQ=255)
                                                                                                                              cTGGGCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCa                     <  2:2075671/151‑1 (MQ=255)
                                                                                                                                gggCTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGc                   <  1:3717218/151‑1 (MQ=255)
                                                                                                                                   cTTCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGc                <  1:1679802/151‑1 (MQ=255)
                                                                                                                                    ttCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGcc               <  1:1412096/151‑1 (MQ=255)
                                                                                                                                     tCGGCGGTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCa              <  1:3551349/151‑1 (MQ=255)
                                                                                                                                      cggcggTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAg             >  1:2417052/1‑151 (MQ=255)
                                                                                                                                         cggTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAggtg          <  2:833359/151‑1 (MQ=255)
                                                                                                                                         cggTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAggtg          <  2:833575/151‑1 (MQ=255)
                                                                                                                                          ggTGGAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAggtgg         >  2:5078417/1‑151 (MQ=255)
                                                                                                                                             ggAACCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAGGTGGTcc      >  1:2036612/1‑151 (MQ=255)
                                                                                                                                               aaCCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAGGTGGTCCAg    <  2:1663370/151‑1 (MQ=255)
                                                                                                                                               aaCCATCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAGGTGGTCCAg    >  2:2475791/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGCAGCAGACGGTGTTTGCCTGGGTGTGCTGGCAGGGCCACTTCCGGGAAGTTGACCGAGGACACCGAAGTACCGTTGTCGCTGTACTTGACCAGTTTCTCGGCCACTTCCAGGCCGATGTTGGCCTGGGCTTCGGCGGTGGAACCACCAATGTGCGGCGTCAGGATCACGTTGTCGAGGCCGCGCAGCGGGCTTTCGAACTCTTCGTCGTTGGAGCGTGGCTCGACCGGGAACACGTCGATGGCGGCGCCGATCAGGTGCTTGTCCTTGATGGCAGCGGCCAGGTGGTCCAGCT  >  CP009974/2393728‑2394023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑