BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	2,738,513	G→A	K242K (AAG→AAA)	RPPX_12165 →	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	2,738,513	0	G	A	98.9%	410.6 / NA	181	K242K (AAG→AAA)	RPPX_12165	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (78/100); minor base C (0/2); total (78/103)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

CGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAG  >  CP009974/2738369‑2738660

tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                                                                                               <  1:2224635/144‑1 (MQ=1)

   agctaGCTCCTACGTCTACGAAGGTAACTGCAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc                                                                                                                                            <  2:2485100/147‑2 (MQ=1)

   agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc                                                                                                                                            <  1:3911754/147‑2 (MQ=1)

    gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                                           <  1:172489/148‑3 (MQ=1)

    gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                                           >  1:4934264/4‑149 (MQ=1)

    gctaGCTCCTACAACTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGAAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                                           <  1:5227194/148‑3 (MQ=1)

          tcctACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGgct                                                                                                                                     <  1:4932243/148‑2 (MQ=0)

                     ggagggtaaCGGGAAGCTGACCGCCGAGAACGGGGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCCAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAg                                                                                                                          <  2:3519538/144‑1 (MQ=0)

                                              gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGGGGTGGTTTCTATTCCTTCg                                                                                                 >  2:1060810/4‑151 (MQ=1)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAGACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           <  2:3635164/151‑1 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           <  2:5111156/151‑1 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           >  1:1218859/1‑151 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           <  2:5110706/151‑1 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           <  2:3353310/151‑1 (MQ=2)

                                                     gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                                          <  2:3115691/151‑1 (MQ=11)

                                                     gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                                          >  1:446442/1‑151 (MQ=2)

                                                       gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac                                                                                        <  1:1947194/151‑1 (MQ=2)

                                                       gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac                                                                                        <  2:3890259/151‑1 (MQ=2)

                                                            cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg                                                                                   <  2:1074243/151‑1 (MQ=2)

                                                            cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg                                                                                   <  1:1018359/151‑1 (MQ=2)

                                                              gCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctgct                                                                                 <  2:2240588/151‑1 (MQ=2)

                                                                  tcatgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctc                                                                             <  2:1249060/144‑1 (MQ=2)

                                                                  tcatgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctc                                                                             >  1:5151866/8‑151 (MQ=11)

                                                                   catgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctct                                                                            >  2:27921/7‑151 (MQ=11)

                                                                        taGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACt                                                                       <  2:951691/150‑1 (MQ=14)

                                                                          gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                                     <  1:4007105/151‑1 (MQ=14)

                                                                               gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                                >  2:4263949/1‑151 (MQ=14)

                                                                                 gCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCg                                                              >  1:3322156/1‑151 (MQ=14)

                                                                                   aCTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTGAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGaa                                                            <  2:1656047/151‑1 (MQ=14)

                                                                                   aCTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGaa                                                            >  1:2909584/1‑151 (MQ=14)

                                                                                    cTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAc                                                           >  1:3418908/1‑151 (MQ=17)

                                                                                    cTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAc                                                           >  1:1516255/1‑151 (MQ=17)

                                                                                       gAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcccc                                                        >  2:1291132/1‑151 (MQ=14)

                                                                                        aaCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCg                                                       <  2:624736/151‑1 (MQ=17)

                                                                                        aaCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCg                                                       <  1:2510936/151‑1 (MQ=14)

                                                                                         aCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGa                                                      <  1:1609330/151‑1 (MQ=17)

                                                                                             cgccgcCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGAt                                                  <  2:77199/151‑1 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGTGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 >  1:2275347/4‑151 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 <  1:2376464/148‑1 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 >  1:3026126/4‑151 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 <  2:4029910/148‑1 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 <  1:419890/148‑1 (MQ=17)

                                                                                               ccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAACACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCg                                                <  2:2704931/149‑1 (MQ=17)

                                                                                                cgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGc                                               <  2:1442260/150‑1 (MQ=17)

                                                                                                cgCCACGCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAACTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGc                                               <  2:4232604/150‑1 (MQ=17)

                                                                                                 gCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                                              <  1:3212642/151‑1 (MQ=18)

                                                                                                 gCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                                              >  1:5229121/1‑151 (MQ=17)

                                                                                                  ctactcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGGCTCGGTGGGCGGACCCCGAGGATCGccc                                             >  2:2990509/6‑151 (MQ=17)

                                                                                                  ccACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGccc                                             >  1:4486306/1‑151 (MQ=18)

                                                                                                   cACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                                            <  1:1008482/151‑1 (MQ=18)

                                                                                                   cACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGACGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                                            <  2:1887583/151‑1 (MQ=18)

                                                                                                       cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCACGAGGATCGCCCCGCtt                                        >  2:4828671/1‑151 (MQ=17)

                                                                                                        aGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTa                                       <  2:1192116/151‑1 (MQ=18)

                                                                                                        aGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTa                                       >  1:2701117/1‑151 (MQ=18)

                                                                                                           aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                                    <  1:856191/151‑1 (MQ=255)

                                                                                                           aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                                    <  2:1210318/151‑1 (MQ=255)

                                                                                                           aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                                    <  2:1381787/151‑1 (MQ=255)

                                                                                                           aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                                    <  2:4090766/151‑1 (MQ=255)

                                                                                                           aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGCTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                                    <  2:1597764/151‑1 (MQ=255)

                                                                                                            ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                                   >  2:1215765/1‑151 (MQ=18)

                                                                                                             cAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgc                                  <  1:1437713/151‑1 (MQ=255)

                                                                                                               gcgcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcgc                                <  2:4375933/151‑1 (MQ=255)

                                                                                                               gcgcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcgc                                <  2:2476848/151‑1 (MQ=255)

                                                                                                                 gcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCg                              >  1:2666195/1‑151 (MQ=18)

                                                                                                                  cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt                             <  1:3115836/151‑1 (MQ=18)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTCTGAGCGCCGTg                            >  2:1049734/1‑151 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            >  2:4455281/1‑151 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            <  1:3005224/151‑1 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            >  1:5126179/1‑151 (MQ=255)

                                                                                                                     gcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGCACACCTGCTGCTCGGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAc                          >  2:5014632/1‑151 (MQ=18)

                                                                                                                       agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa                        <  1:3566522/151‑1 (MQ=255)

                                                                                                                       agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa                        <  2:2978050/151‑1 (MQ=255)

                                                                                                                        gcgcGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCaa                       <  1:4779026/151‑1 (MQ=255)

                                                                                                                          gcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAtt                     >  2:4694484/1‑151 (MQ=255)

                                                                                                                          gcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAtt                     >  1:4071660/1‑151 (MQ=255)

                                                                                                                           cgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTg                    >  1:2722232/1‑151 (MQ=255)

                                                                                                                            caggcaggcGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTgg                   <  1:4279759/150‑1 (MQ=255)

                                                                                                                              ggcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCt                 >  1:619621/8‑151 (MQ=255)

                                                                                                                                caggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGGTCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCa               >  2:3258392/6‑151 (MQ=255)

                                                                                                                                caggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCa               >  1:5070949/6‑151 (MQ=255)

                                                                                                                                 aggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCaa              >  1:1169797/5‑151 (MQ=21)

                                                                                                                                 aggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCaa              >  2:2392399/5‑151 (MQ=21)

                                                                                                                                  ggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAg             <  1:1812855/148‑1 (MQ=21)

                                                                                                                                    cgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAt           <  1:2649930/150‑1 (MQ=255)

                                                                                                                                    cgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAt           >  1:4669670/2‑151 (MQ=255)

                                                                                                                                    cgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAt           <  2:260480/150‑1 (MQ=255)

                                                                                                                                     gACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAtt          >  2:1516495/1‑151 (MQ=21)

                                                                                                                                      aCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAttt         >  1:2821838/1‑151 (MQ=255)

                                                                                                                                      aCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAttt         <  1:4890530/151‑1 (MQ=25)

                                                                                                                                      aCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAACTGGCTCAAGAttt         >  1:4495904/1‑151 (MQ=255)

                                                                                                                                       cGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTc        <  1:3630754/151‑1 (MQ=255)

                                                                                                                                        gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCg       >  2:3280083/4‑151 (MQ=255)

                                                                                                                                         aaaTCAAAACCGTGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCgg      <  2:3881051/149‑1 (MQ=255)

                                                                                                                                          aaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCCATTGGCTCAAGATTTCGGc     >  2:5215427/2‑151 (MQ=255)

                                                                                                                                           aTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGcc    <  2:1958324/151‑1 (MQ=255)

                                                                                                                                            tCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCa   <  2:505147/151‑1 (MQ=255)

                                                                                                                                             cAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAg  >  1:4069194/1‑151 (MQ=25)

                                                                                                                                             cAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAg  <  2:3431968/151‑1 (MQ=255)

                                                                                                                                             cAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAg  <  1:3845017/151‑1 (MQ=255)

                                                                                                                                             cAAAACCATGAGCGCCGGCTCTTGAGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAg  <  1:471526/151‑1 (MQ=255)

CGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAG  >  CP009974/2738369‑2738660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`