Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,899,147 T→C V329A (GTG→GCG)  RPPX_12905 → DEAD/DEAH box helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,899,1470TC99.3% 967.1 / ‑4.8 277V329A (GTG→GCG) RPPX_12905DEAD/DEAH box helicase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (142/133);  total (142/135)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

CAGGCGACCCGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGTGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCATCCCCGA  >  CP009974/2898999‑2899294
                                                                                                                                                    |                                                                                                                                                   
caGGCGACCCGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACgcgc                                                                                                                                                   >  2:3977669/1‑151 (MQ=255)
  ggCGACCCGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCAc                                                                                                                                                 >  1:3186464/1‑151 (MQ=255)
     gACCCGCCAGCGTGCGCTGGCCAGCTTGAAGGCCCGTGAGGTGGGAGAGCGGGTAGCGCCCGGTGGGGCGCCCCTTGGCCAGAATAAAGATGACTTGCCGCTCGTCCTCAACCTCGACCTGCCGATCGTTGCCCAAGACTACGCGcccccc                                                                                                                                              >  2:3713174/1‑149 (MQ=255)
     gACCCGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGc                                                                                                                                              >  1:3971267/1‑151 (MQ=255)
        ccGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACGGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATc                                                                                                                                           <  2:2845494/151‑1 (MQ=255)
        ccGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATc                                                                                                                                           <  2:343131/151‑1 (MQ=255)
        ccGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATc                                                                                                                                           <  2:429551/151‑1 (MQ=255)
          gCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCgg                                                                                                                                         >  1:2895057/1‑151 (MQ=255)
          gCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCgg                                                                                                                                         >  2:875361/1‑151 (MQ=255)
            cAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGc                                                                                                                                       >  1:2384763/1‑151 (MQ=255)
               cGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCGGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTa                                                                                                                                    <  2:1775035/151‑1 (MQ=255)
                gTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTAc                                                                                                                                   >  2:4867925/1‑151 (MQ=255)
                 ggcgcTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTAcc                                                                                                                                  <  2:3423334/150‑1 (MQ=255)
                  gcgcTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACcg                                                                                                                                 >  2:1912180/1‑151 (MQ=255)
                    gcTGGACAGCTTCAACGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTCGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCGGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCTTACctgg                                                                                                                               <  2:323492/151‑1 (MQ=255)
                     cTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACcgggc                                                                                                                              >  1:4840956/1‑151 (MQ=255)
                         aCAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACcgggcgggc                                                                                                                          >  2:718682/1‑151 (MQ=255)
                             cTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCgggcgggcgggc                                                                                                                      <  2:802609/151‑1 (MQ=255)
                               tCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGcaa                                                                                                                    >  2:2518881/1‑151 (MQ=255)
                               tCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGcaa                                                                                                                    <  1:2876367/151‑1 (MQ=255)
                                 aaGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGcaaca                                                                                                                  <  2:2433860/151‑1 (MQ=255)
                                  aGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGcaacaa                                                                                                                 <  1:2860024/151‑1 (MQ=255)
                                  aGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGcaacaa                                                                                                                 <  1:2772897/151‑1 (MQ=255)
                                  aGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGcaacaa                                                                                                                 >  2:4134130/1‑151 (MQ=255)
                                   ggCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAg                                                                                                                <  1:2626804/151‑1 (MQ=255)
                                    gCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAgg                                                                                                               >  1:5084136/1‑151 (MQ=255)
                                    gCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAgg                                                                                                               >  2:4269578/1‑151 (MQ=255)
                                      ccGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGCCCTGCCGATCGTTGCCGAAGACTCCGCGCCCCGCATCGGGCGTACCCGGCGGGCGCGCAACCAggcc                                                                                                             >  2:5229181/1‑149 (MQ=255)
                                        gTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGggcga                                                                                                           <  2:3291310/151‑1 (MQ=255)
                                          gAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACTCGCACCGCATTTGGCGTACCGGGCGGGCGGGCAACAAGggcgagg                                                                                                         >  1:410278/1‑151 (MQ=255)
                                          gAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGggcgagg                                                                                                         >  2:1154508/1‑151 (MQ=255)
                                           aGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGggcgaggc                                                                                                        <  1:875361/151‑1 (MQ=255)
                                           aGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGggcgaggc                                                                                                        <  1:2282978/151‑1 (MQ=255)
                                             gTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGggcgaggcga                                                                                                      <  1:2072555/151‑1 (MQ=255)
                                              tGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTGCCGGGCGGGCGGGCAACAAGGGCGAGCCGAt                                                                                                     >  2:3306378/1‑151 (MQ=255)
                                                     gCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTg                                                                                              >  2:3284180/1‑151 (MQ=255)
                                                       tggtggCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGgt                                                                                            >  1:1928799/1‑151 (MQ=255)
                                                        ggtggCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGgtg                                                                                           <  1:211823/151‑1 (MQ=255)
                                                         gtggCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGgtgt                                                                                          <  1:3333462/151‑1 (MQ=255)
                                                         gtggCGACCGATGTGGCGGCCCGTGGCCTAGATATCGATTACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGGCTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGgtgt                                                                                          <  2:2554946/151‑1 (MQ=255)
                                                           ggCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGgtgtgt                                                                                        <  2:4585490/151‑1 (MQ=255)
                                                             cGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGc                                                                                      <  2:2280863/151‑1 (MQ=255)
                                                              gACCGAGGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCg                                                                                     <  2:1007563/151‑1 (MQ=255)
                                                                   aTGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGa                                                                                <  1:3831295/151‑1 (MQ=255)
                                                                     gtgGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAg                                                                              <  1:3621340/151‑1 (MQ=255)
                                                                       ggcggcCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTg                                                                            >  2:1367993/1‑151 (MQ=255)
                                                                           gcCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGTGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAAc                                                                        <  2:3483474/151‑1 (MQ=255)
                                                                            cccGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAAct                                                                       >  2:1613537/1‑151 (MQ=255)
                                                                             ccGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAActg                                                                      >  2:3795510/1‑151 (MQ=255)
                                                                             ccGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAActg                                                                      >  1:3686926/1‑151 (MQ=255)
                                                                               gTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAActgct                                                                    >  2:277338/1‑151 (MQ=255)
                                                                                tGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACTTGCCGATTGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGCGTGTGCGGATGAAGTGCAActgctg                                                                   >  1:1863310/1‑151 (MQ=255)
                                                                                tGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAActgctg                                                                   >  1:3550714/1‑151 (MQ=255)
                                                                                   ccTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTggcg                                                                <  1:971524/151‑1 (MQ=255)
                                                                                    cTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTggcgg                                                               <  1:4355881/151‑1 (MQ=255)
                                                                                     tGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTggcggc                                                              <  2:5036302/151‑1 (MQ=255)
                                                                                     gggATATGGATGACTTGCCGCTGGTCGTCAACCGCGGCCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTggcggc                                                              <  2:2378113/150‑1 (MQ=255)
                                                                                      ggATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCt                                                             <  1:3515383/151‑1 (MQ=255)
                                                                                      ggATATCGATGACTTGCCGCGGGTCGGCAGCCTCGACCTGCGGATCGTGGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCt                                                             <  2:5150191/151‑1 (MQ=255)
                                                                                       gATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTa                                                            >  2:3616921/1‑151 (MQ=255)
                                                                                       gATATCGATGACTTGCCCCTGGTCGTCAACCTCGACCTGCCGACCGTTGCCGAAGACTACGCGCACCGCATCCGGCCCACCGCGCGGGCGCGCAACAAGGGCGCGGCGATTTCCCTTGTCTGTACGGAAGAAGAGCAACTGCTGGCGGCTa                                                            >  2:3814570/1‑151 (MQ=255)
                                                                                        atatCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTAt                                                           <  1:2354553/151‑1 (MQ=255)
                                                                                         tgtCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATc                                                          <  2:4410657/149‑1 (MQ=255)
                                                                                            cGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAg                                                       >  1:1980662/1‑151 (MQ=255)
                                                                                             gATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAgg                                                      <  1:2218820/151‑1 (MQ=255)
                                                                                             gATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAgg                                                      >  2:1069684/1‑151 (MQ=255)
                                                                                             gATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAgg                                                      >  1:4561175/1‑151 (MQ=255)
                                                                                              aTGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGt                                                     <  1:3877901/151‑1 (MQ=255)
                                                                                               tGACTGGCCGCTGGTCGTCAACCTCGACCTGCCGACCGTTGCGGAAGACTACGCGCACAGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTg                                                    <  2:1479016/151‑1 (MQ=255)
                                                                                                    tGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGAc                                               >  1:1387430/1‑151 (MQ=255)
                                                                                                    tGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGAc                                               <  1:1013678/151‑1 (MQ=255)
                                                                                                       cGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGACGTGCAACTGCTGGCGGCCATCGAGGTGCTGACCCg                                            >  2:3912292/1‑151 (MQ=255)
                                                                                                       cGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCg                                            >  2:310507/1‑151 (MQ=255)
                                                                                                        gCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCgg                                           >  1:4215335/1‑151 (MQ=255)
                                                                                                        gCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCgg                                           <  1:4045400/151‑1 (MQ=255)
                                                                                                        gCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCgg                                           <  1:823281/151‑1 (MQ=255)
                                                                                                          tGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCa                                         >  1:1244959/1‑151 (MQ=255)
                                                                                                          tGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCa                                         >  2:300908/1‑151 (MQ=255)
                                                                                                           ggTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAg                                        <  1:1745248/151‑1 (MQ=255)
                                                                                                              cgtcAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCTCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGAcc                                     >  1:3942274/1‑151 (MQ=255)
                                                                                                                tcAACCTCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCt                                   <  1:1496953/151‑1 (MQ=255)
                                                                                                                  aaCCTCGACCTGCCGATCGTTGCCGAAGCCTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGc                                 <  1:5163643/151‑1 (MQ=255)
                                                                                                                   aCCTGGACCTGCCGATCGTTGGCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGcc                                <  2:85049/151‑1 (MQ=255)
                                                                                                                   aCCTCGACCTGCCGATCGGTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGcc                                <  1:4707981/151‑1 (MQ=255)
                                                                                                                      tCGACCTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCcgcg                             >  2:594597/1‑151 (MQ=255)
                                                                                                                           cTGCCGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATg                        <  1:4414270/151‑1 (MQ=255)
                                                                                                                              cggATCGGTGCCGAAGAGTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAag                     <  2:3180462/149‑1 (MQ=255)
                                                                                                                               cGATCGTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAaga                    >  2:2317997/1‑151 (MQ=255)
                                                                                                                                  tCGCTGCCGAAGACTCCGCGCCCCGCATCGGGCGTACCGGGCGGGGGGGCACCAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCACCTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGcc                 <  2:196235/151‑1 (MQ=255)
                                                                                                                                    gTTGCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCgg               >  2:2129066/1‑151 (MQ=255)
                                                                                                                                       gCCGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGAtt            <  2:2881750/151‑1 (MQ=255)
                                                                                                                                       gCCGAAGACGACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGAtt            <  1:4773215/151‑1 (MQ=255)
                                                                                                                                         ggAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTc          <  2:917162/150‑1 (MQ=255)
                                                                                                                                         cGAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTc          >  2:4489918/1‑151 (MQ=255)
                                                                                                                                          gAAGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCa         >  1:1100882/1‑151 (MQ=255)
                                                                                                                                            aGACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCCTc       >  2:2678955/1‑151 (MQ=255)
                                                                                                                                             gACTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCATcc      <  1:1613537/151‑1 (MQ=255)
                                                                                                                                              aCTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCATccc     <  1:4888225/151‑1 (MQ=255)
                                                                                                                                               cTACGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCATcccc    <  2:3754748/151‑1 (MQ=255)
                                                                                                                                                 aCGCGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCATCCCCGa  >  2:17769/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGGCGACCCGCCAGCGTGCGCTGGACAGCTTCAAGGCCCGTGAGGTGCAAGTGCTGGTGGCGACCGATGTGGCGGCCCGTGGCCTGGATATCGATGACTTGCCGCTGGTCGTCAACCTCGACCTGCCGATCGTTGCCGAAGACTACGTGCACCGCATCGGGCGTACCGGGCGGGCGGGCAACAAGGGCGAGGCGATTTCGCTGGTGTGTGCGGATGAAGTGCAACTGCTGGCGGCTATCGAGGTGCTGACCCGGCAGACCCTGCCGCGTCATGAAGAGCCGGATTTCATCCCCGA  >  CP009974/2898999‑2899294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑