Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,900,811 A→G P9P (CCT→CCC)  RPPX_12910 ← NAD(FAD)‑utilizing dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,900,8110AG90.1% 619.1 / 35.0 228P9P (CCT→CCC) RPPX_12910NAD(FAD)‑utilizing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (10/11);  new base G (94/97);  total (113/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GATGTTCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTCG  >  CP009974/2900664‑2900957
                                                                                                                                                     |                                                                                                                                                  
gATGTTCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑gggggggggg                                                                                                                                                 >  1:4103650/1‑151 (MQ=255)
 aTGTTCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑Ggggggggggg                                                                                                                                                >  1:912172/1‑151 (MQ=255)
   gTTCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCCGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGggcgaggggct                                                                                                                                              >  2:2953270/1‑149 (MQ=255)
    ttCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGgggggggggagg                                                                                                                                             >  2:2399420/1‑149 (MQ=255)
    ttCATGCCGCCGACACCCGCCAGCAGGAACTGGCGGCCCACCGAAGCGATGGCATCGAAGGGATCGACCGCCAGGCCTGCCCTGGCCCGTGCTTCGGCAGCGATCACGCCGGTGGGGCCGCCACCGATGACGCACGC‑‑GGgggaggggggcg                                                                                                                                             >  2:3942361/1‑148 (MQ=255)
     tCATGCCGCCGACACCGGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTGGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCAACAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGc                                                                                                                                            <  1:976725/151‑1 (MQ=255)
     tCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGc                                                                                                                                            <  1:4830068/151‑1 (MQ=255)
          ccgccgACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGGTGCggggg                                                                                                                                       >  2:3120987/1‑151 (MQ=255)
           cgccgACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCTTGCACTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCCCCGATGTCGACAGC‑‑GGGGGGGGGGGATGCgggggc                                                                                                                                      >  2:2551915/1‑150 (MQ=255)
           cgccgACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCTATGACGACAGC‑‑GGGGGGGGGGGGTGCgggggg                                                                                                                                      >  1:2313483/1‑150 (MQ=255)
           cgccgACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGG‑GGGGATGCGGGGGTc                                                                                                                                     >  1:3107450/1‑151 (MQ=255)
              cgACACCGGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCGGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGG‑GGGGATGCGGGGGTCAGa                                                                                                                                  <  2:1873866/151‑1 (MQ=255)
                acacCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGAt                                                                                                                                 >  1:3572876/1‑151 (MQ=255)
                 cacCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGG‑‑GCGGGGGTCAGATCgg                                                                                                                              >  1:615403/1‑151 (MQ=255)
                  acCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGG‑GGGGATGCGGGGGTGAGATCgg                                                                                                                              >  1:3196173/1‑151 (MQ=255)
                   cccGCCAGCAGGAACTTGCGGCCCACTGAAGGCAGGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGGGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGAAAGG‑‑GGGGGGGGGGGATGCGGGGGTCAGATCgg                                                                                                                              <  2:885352/151‑1 (MQ=255)
                      gCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGCGGGGGGGGGGGGGAGGCGGGGGGCCGATCggg                                                                                                                             >  1:56667/1‑151 (MQ=255)
                      gCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGATCGGGCa                                                                                                                           >  1:3663853/1‑151 (MQ=255)
                        cagcagGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCagg                                                                                                                         >  1:1894898/1‑149 (MQ=255)
                         agcagGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGGTGCGGGGGGCAGATCGGGCAtgg                                                                                                                        >  1:3382540/1‑151 (MQ=255)
                         agcagGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCAtgg                                                                                                                        >  2:782544/1‑151 (MQ=255)
                          gcagGACCTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCAtggt                                                                                                                       <  1:508097/151‑1 (MQ=255)
                          gcagGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGGTGCGGGGGTCAGATCGGGCAtggg                                                                                                                       >  2:682810/1‑150 (MQ=255)
                           cagGACCTGGGGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCAtggtg                                                                                                                      <  1:3497303/151‑1 (MQ=255)
                            gggAAGTTGCGGCCCACGGGGGGCATGGCATCGAAGACCGCGCCCGCCAGGCCGGCCCTGGCCGGTGCTGGGGCAGCCAGCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCAtggtgg                                                                                                                     <  2:3161166/150‑1 (MQ=255)
                            agGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGAGGGGGGGGTCAGAGCGGGcgcggggg                                                                                                                     >  2:2415481/1‑144 (MQ=255)
                              gAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGCCCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGgc                                                                                                                   <  2:4648060/151‑1 (MQ=255)
                              gAACGTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGgc                                                                                                                   <  1:423768/151‑1 (MQ=255)
                               agcTTGCGGCCCACTGAGGGCATGGCATCGAAGACCGCGACCGCCAGGCCGGCCCTGGCCAGGGCTTCGGCAGCCAGCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGgca                                                                                                                  <  2:2426871/149‑1 (MQ=255)
                                  ttGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGGCAGATCGGGCATGGGGGgcagca                                                                                                               >  1:1734565/1‑151 (MQ=255)
                                   ggCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGcagcag                                                                                                              <  1:2713255/150‑1 (MQ=255)
                                    gCGGCCCACTGAAGCCATGGAATCGAAGCCCTCGCCCGCCAGCCCTGCCCGGGCCAGGGCTTCGGAAGCCAAAGGGCCGGGGGGGCCGCAACCGATGACGACAGCG‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGcagcag                                                                                                              <  2:561324/151‑1 (MQ=255)
                                     ccgCCCACTGAAGGCATGGCATCGAAGCCCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGc                                                                                                            <  1:3209583/149‑1 (MQ=255)
                                       gCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGCG‑GGGGGGGGGGGGTGCGGGGGGCAGACGGGGCAGGGGGGGCCGCAGGcg                                                                                                           >  2:3395607/1‑150 (MQ=255)
                                       gCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGACCGGGCACGGTGGGCAACAGGCtg                                                                                                          >  2:3221800/1‑151 (MQ=255)
                                         ccgcTGAGGGCATGGCGGCGAGGGCGTCGGCCGCCAGGCCTGCCCTGGCCAGTGCTGCGGCAGCCAGCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCtgtg                                                                                                        <  2:3859785/148‑1 (MQ=255)
                                            cggAAGGCATGGCATCGAAGCCCTCGACCCCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGaa                                                                                                     <  1:1235329/149‑1 (MQ=255)
                                                         atCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGTGGGGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGGGCATTc                                                                                        >  1:1566140/1‑151 (MQ=255)
                                                          tCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGGGGGGGGCAGGTCGGGCGTGGCGGGCAGCAGGCCGTGGAAACGAGGCGCATTcc                                                                                       >  1:4989493/1‑150 (MQ=255)
                                                           cGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTa                                                                                      >  2:1673133/1‑151 (MQ=255)
                                                           cGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGGGGAAAAGAGGCGCATTCTa                                                                                      >  2:4209540/1‑151 (MQ=255)
                                                            gAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTAc                                                                                     >  1:4484366/1‑151 (MQ=255)
                                                            gAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGCGGGTCGGATCGGGGCGGGGGGGCAGCCGGATGCGGAAAACAGGCGCGTTcgcc                                                                                     >  2:801359/1‑148 (MQ=255)
                                                             aaGACCCGGCCCGCCAGGCCGGCCCTGGCCAGTGCTGGGGCAGCCATCAGGCCGGGGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTAcc                                                                                    <  2:937772/151‑1 (MQ=255)
                                                               gACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGCATGCGGGGGTCAGATCGGGCATGGGGGGCAGCAGGCTGCGGAAAAGAGGCGCATTCTAcccg                                                                                  >  2:3782706/1‑149 (MQ=255)
                                                                       ccGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGc                                                                          >  1:572503/1‑151 (MQ=255)
                                                                       ccGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGGCAGATCGGGCATGGTGGGGAGCAGGCTGTGGAAAAAAGGCGCATTCTACCGCatggccgc                                                                          >  1:2825564/1‑144 (MQ=255)
                                                                         gCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGCG‑GGGGGGGGGGGGTGGGGAGGCCAGCGCGGGCCAGGGGGGCAGCCGGGGGGGGAGAGGGGGGCCATCCTACGCCCGCGCTGcc                                                                         >  2:5017909/1‑151 (MQ=255)
                                                                         gCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGGCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGccc                                                                        >  1:2169017/1‑151 (MQ=255)
                                                                          cggggCGTGCCCGGGCCAGGGCTGCGGCAGCCAGCAGGGCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCa                                                                       <  2:4521387/148‑1 (MQ=255)
                                                                            gggCGTGCCCTGGCGAGTGCTTGGGGAGCCATGGGGCGGGCGGGGCGGCCCCGGAGGGCGAAGGG‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATg                                                                     <  2:4963642/150‑1 (MQ=255)
                                                                             ggccggccCGGGCCAGGGGTCGGGGGGCCAGCGGGCGGGGGGGGCCGCCACGGAGGACGAGAGGG‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATg                                                                     <  2:2342799/151‑1 (MQ=255)
                                                                              gCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGa                                                                   >  2:4692413/1‑151 (MQ=255)
                                                                                     gtGGCCGGTGCTTCGGCAGCCATCGGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTg                                                            <  1:1313865/150‑1 (MQ=255)
                                                                                     cTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGCG‑GGGGGGGGGGGGTGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGGGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACt                                                             >  1:2512188/1‑151 (MQ=255)
                                                                                     cTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTg                                                            >  2:2218282/1‑151 (MQ=255)
                                                                                      tGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTgg                                                           >  2:1373060/1‑151 (MQ=255)
                                                                                      tGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTgg                                                           >  1:2114364/1‑151 (MQ=255)
                                                                                      tGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTgg                                                           <  2:3510538/151‑1 (MQ=255)
                                                                                         cccgTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCa                                                        <  1:3824986/148‑1 (MQ=255)
                                                                                         ccAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCAAGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCa                                                        >  2:148136/1‑151 (MQ=255)
                                                                                           gggcgtgcGGCAGCCCGCAGGGCGGGGGGGCCGCCACCGAGAACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCaaa                                                      <  2:2844682/144‑1 (MQ=255)
                                                                                           aGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGGGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCaaa                                                      >  2:3198067/1‑151 (MQ=255)
                                                                                             tGCTACGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCaaaaa                                                    <  1:3312517/151‑1 (MQ=255)
                                                                                              gCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCaaaaaa                                                   <  1:1693678/151‑1 (MQ=255)
                                                                                               cgggggCAGCCATGAGGCCGGCGGGGCCGCCGCGGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGACAAAAAAt                                                  <  2:2331124/147‑1 (MQ=255)
                                                                                                ttgggCAGCCATGAGGCCGGCGGGGCCGCGCCCGATGAGGGAAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATa                                                 <  1:4312393/148‑1 (MQ=255)
                                                                                                ttCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGGCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATa                                                 >  2:2017234/1‑151 (MQ=255)
                                                                                                tgcGGCAGCGATCGGGCGGGCGGGGCCGCCACGGATGACGACAGGG‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAAt                                                  <  1:796533/149‑1 (MQ=255)
                                                                                                     gaggcATGAGGCCGGCGGGGCCGCCACGGAGGACGAGGGG‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAg                                            <  2:4754560/147‑1 (MQ=255)
                                                                                                         cagcAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTc                                        <  1:1658526/148‑1 (MQ=255)
                                                                                                          agcAGGCCGGCGGGGGCGCCACCGATGGCGACGGG‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCa                                       <  1:1082600/149‑1 (MQ=255)
                                                                                                          aTCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCa                                       <  1:561619/151‑1 (MQ=255)
                                                                                                           gcgggCGGGGGGGGCCGCCACCGATGGCGAGAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCAc                                      <  2:4475068/148‑1 (MQ=255)
                                                                                                            cAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACg                                     <  1:2121179/151‑1 (MQ=255)
                                                                                                             aGGCCGGGGGGGCCGCCACCGGTGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGc                                    <  1:2422415/151‑1 (MQ=255)
                                                                                                                cgggaggggCCGCCACCGATGACGACGGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAgg                                 <  2:285847/146‑1 (MQ=255)
                                                                                                                 cggcggGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGc                                <  1:4858426/151‑1 (MQ=255)
                                                                                                                  ggcggGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGcc                               >  1:4945396/1‑151 (MQ=255)
                                                                                                                    gggggCCGCCAACGATGAAGAAAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCtt                             <  1:2179399/150‑1 (MQ=255)
                                                                                                                    cggGGCCGCCACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCGGCCCATGCAACCACTGGTCAAAAAATAGCCAGTTTCACGCAGGCCtt                             >  2:3941828/1‑151 (MQ=255)
                                                                                                                         ccgccACCGATGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAg                        <  1:723524/151‑1 (MQ=255)
                                                                                                                           gccACCGATGACGACGGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTa                      <  1:4085772/151‑1 (MQ=255)
                                                                                                                            ccACCGATGACGACAGC‑‑GGGGGG‑GGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTaaa                    <  2:3107450/151‑1 (MQ=255)
                                                                                                                              cccGAAGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAg                   <  1:2272813/150‑1 (MQ=255)
                                                                                                                               ccGAGGACGACAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAgg                  <  1:2976626/151‑1 (MQ=255)
                                                                                                                                 gATGACGTCAGC‑‑GGGGGGGGGGGATGGGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGc                <  1:4802275/151‑1 (MQ=255)
                                                                                                                                   tGAGGACGGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTg              <  1:3269566/151‑1 (MQ=255)
                                                                                                                                     gggacAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCa            <  2:4063337/149‑1 (MQ=255)
                                                                                                                                       gacAGC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAgc          >  2:1833076/1‑151 (MQ=255)
                                                                                                                                          aGC‑‑GGGGGG‑GGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCt      >  2:1005157/1‑151 (MQ=255)
                                                                                                                                           gC‑‑GGGGGGGGGGGATGCGGGGGTCAGATCGGGCGTGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCt      <  2:2712047/151‑1 (MQ=255)
                                                                                                                                             ggggggggggggGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCt      <  2:2904142/149‑1 (MQ=255)
                                                                                                                                               ggggggggggGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTGCCGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCttt    >  2:4604626/1‑151 (MQ=255)
                                                                                                                                               ggggggggggGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCttt    <  2:2178693/151‑1 (MQ=255)
                                                                                                                                                ggggggggggATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCTAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTc   <  2:4901334/151‑1 (MQ=255)
                                                                                                                                                ggggggggggATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTc   <  1:2551915/151‑1 (MQ=255)
                                                                                                                                                gggggAGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTc   <  1:4574658/151‑1 (MQ=255)
                                                                                                                                                 gggggggggaTGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTcg  <  1:5120962/151‑1 (MQ=255)
                                                                                                                                                 gggggggggaTGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTcg  <  1:1525151/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GATGTTCATGCCGCCGACACCCGCCAGCAGGAACTTGCGGCCCACTGAAGGCATGGCATCGAAGACCTCGACCGCCAGGCCTGCCCTGGCCAGTGCTTCGGCAGCCATCAGGCCGGCGGGGCCGCCACCGATGACGACAGC‑‑GGGGGGAGGGGATGCGGGGGTCAGATCGGGCATGGTGGGCAGCAGGCTGTGGAAAAGAGGCGCATTCTACCGCAGCGCTGCCCATGGAAGCACTGGTCAAAAAATAGCCAGTGTCACGCAGGCCTTGCCAGTAAAGGGCTGCAGCGCCTTTCG  >  CP009974/2900664‑2900957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑