Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,910,161 T→C intergenic (+65/‑594) gudD → / → RPPX_12965 glucarate dehydratase/GABA permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,910,1610TC88.3% 798.4 / 62.4 312intergenic (+65/‑594)gudD/RPPX_12965glucarate dehydratase/GABA permease
Reads supporting (aligned to +/- strand):  ref base T (10/26);  new base C (101/172);  total (111/201)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 334 total aligned reads displayed.

GGTGCGCGGGATGACAGCGTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCC  >  CP009974/2910013‑2910311
                                                                                                                                                    |                                                                                                                                                      
   gcgcGGGATGACAGCGTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAgg                                                                                                                                                   <  2:2958546/151‑1 (MQ=255)
        ggATGGCAGCGTGGCGATGCAGTTCCTGATTCGGGGTTGGAGCTTCGATAGCAAGCGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAGGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCgg                                                                                                                                              <  2:4232775/151‑1 (MQ=255)
           tGACAGCGTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGtt                                                                                                                                           <  1:774530/151‑1 (MQ=255)
               aGCGTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCAcc                                                                                                                                       >  1:2935706/1‑151 (MQ=255)
                  gTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCcgc                                                                                                                                    >  1:5206989/1‑151 (MQ=255)
                  gTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCcgc                                                                                                                                    >  2:1784784/1‑151 (MQ=255)
                       gATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATa                                                                                                                               <  1:8127/151‑1 (MQ=255)
                           cAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGcc                                                                                                                           <  1:1793959/151‑1 (MQ=255)
                              ttCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGa                                                                                                                        >  2:4819183/1‑151 (MQ=255)
                              ttCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGa                                                                                                                        <  2:3890334/151‑1 (MQ=255)
                                ccTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGAcc                                                                                                                      <  1:3096074/151‑1 (MQ=255)
                                  tGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTg                                                                                                                    <  1:3479601/151‑1 (MQ=255)
                                    aTTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCCCACCGTCGTAGGCGCGGGGTCACCCGCGCGTCGGCCGCACCTgcc                                                                                                                  >  2:4469480/1‑151 (MQ=38)
                                     ttCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTgccg                                                                                                                 <  2:2205436/151‑1 (MQ=255)
                                           gTTGGGGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAAt                                                                                                           <  2:4948012/151‑1 (MQ=255)
                                               gAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCACTAcct                                                                                                       >  1:3594870/1‑150 (MQ=255)
                                               gAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATAcct                                                                                                       <  2:195208/151‑2 (MQ=255)
                                               gAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATAccc                                                                                                       >  1:3509742/1‑151 (MQ=255)
                                                   ttCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAg                                                                                                   <  2:356015/151‑1 (MQ=255)
                                                    tCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGt                                                                                                  >  1:4694619/1‑151 (MQ=255)
                                                            aaaCGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAAGACCGTCAGGCAGTCAcc                                                                                          >  2:4031116/1‑151 (MQ=37)
                                                             naCGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCAccg                                                                                         >  1:1969065/2‑151 (MQ=255)
                                                               cGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCAccgcc                                                                                       <  1:2309834/151‑1 (MQ=255)
                                                                 gCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACcgccgc                                                                                     <  2:3945214/151‑1 (MQ=255)
                                                                    ttGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCAcc                                                                                  >  2:3766412/1‑151 (MQ=255)
                                                                    tggCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCAcc                                                                                  <  2:1602354/149‑1 (MQ=255)
                                                                     tGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCAccgc                                                                                        <  1:3025106/144‑1 (MQ=255)
                                                                     tGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCAccgc                                                                                        >  2:3025576/1‑144 (MQ=255)
                                                                        cTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGcc                                                                              <  1:2737085/151‑1 (MQ=255)
                                                                           gTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCAcc                                                                           <  1:3278847/151‑1 (MQ=255)
                                                                           gTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCAcc                                                                           <  1:2191606/151‑1 (MQ=255)
                                                                             gCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAg                                                                                                   <  1:2841558/125‑1 (MQ=255)
                                                                                  gAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATtca                                                                    <  1:1025393/151‑1 (MQ=255)
                                                                                   gggCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTCCATCACCCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATtcat                                                                   <  2:1831874/150‑1 (MQ=21)
                                                                                     gCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATtcatca                                                                 <  2:5182698/151‑1 (MQ=255)
                                                                                      cTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCaa                                                                >  1:2853953/1‑151 (MQ=255)
                                                                                       tGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAAt                                                               <  1:893471/151‑1 (MQ=255)
                                                                                         cGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCg                                                             <  2:2854543/151‑1 (MQ=255)
                                                                                            gCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGGATAGGCCGGACCTGCCGGCCAATACCTCCAGTCATTCACCGCCGCACCTCCCACCGCATGCAGCAATCGGcc                                                          >  2:1343787/1‑150 (MQ=37)
                                                                                             ccTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCAc                                                         >  1:3725956/1‑151 (MQ=255)
                                                                                             ccTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCGGCAc                                                         <  2:3371147/151‑1 (MQ=255)
                                                                                              ctctTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCAcc                                                        >  1:1259916/1‑151 (MQ=255)
                                                                                               tctTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATc                                                              >  1:4363596/1‑144 (MQ=255)
                                                                                               tctTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATc                                                              <  2:4363596/144‑1 (MQ=255)
                                                                                                ctTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAAt                                                                                                           <  2:3686910/98‑1 (MQ=255)
                                                                                                ctTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCAccgt                                                      <  2:3497604/151‑3 (MQ=255)
                                                                                                 ttCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTc                                                     <  1:1478761/151‑1 (MQ=255)
                                                                                                 ttCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTc                                                     <  1:3139085/151‑1 (MQ=255)
                                                                                                  tCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCg                                                    >  1:4835188/1‑151 (MQ=255)
                                                                                                  cgccgGGCCCGCCAGCGCATACATCAACCGGCACCGTCGCCCCGCACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCg                                                    <  2:4872556/148‑1 (MQ=11)
                                                                                                   cgggggCAAGCCCGCTCCTACAGCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGc                                                   <  2:3988463/148‑1 (MQ=21)
                                                                                                    gcgGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATAcc                                                                                                        >  2:2453469/1‑97 (MQ=255)
                                                                                                     cgGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCa                                                 >  1:2597026/1‑151 (MQ=255)
                                                                                                     cgGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCa                                                 <  1:4973973/151‑1 (MQ=255)
                                                                                                      gggCAGGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCAc                                                <  2:4301850/151‑1 (MQ=21)
                                                                                                      gggCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCAc                                                <  1:2529487/151‑1 (MQ=25)
                                                                                                      gcgCAGGCCGGCTCCTCCATCAGCCGGTGGTGGCGCCCCACACCGGCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATCCATCAATCGGCACCGTCGCCAc                                                <  2:4655980/149‑1 (MQ=18)
                                                                                                         cAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACgtc                                             >  1:4806/1‑149 (MQ=25)
                                                                                                           ggCCCGCACCTGCAACAACCCGTGGCCTCGCCCCTCACACTCGTAGGCGCGCGTTCACCCTGAAAAAAGCGGGACCAGCCGGCCACTTCCTCCACTGTTTCCCCGCCGCGCCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTAcc                                           <  2:4546149/150‑1 (MQ=255)
                                                                                                             cccGCTACTGCATCAGCCGGTGGTGTCGCCCCACCCCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCAGTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTAccgt                                         <  2:1076462/151‑3 (MQ=18)
                                                                                                              ccGCTCCTACATCACCCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGtt                                        <  2:2093337/151‑1 (MQ=21)
                                                                                                                gCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGt                                      <  2:146081/151‑1 (MQ=21)
                                                                                                                 cTCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTa                                     <  1:1153748/151‑1 (MQ=21)
                                                                                                                  tCCTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAg                                    <  1:5134300/151‑1 (MQ=21)
                                                                                                                  gcattcATCAACCGGCACCGTCGCCCCGCCCCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGTAg                                    <  1:1343787/146‑1 (MQ=17)
                                                                                                                    cTACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGa                                  <  1:4585742/151‑1 (MQ=21)
                                                                                                                    cTACATCAACCGGCGCTGTCGCCACGCACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCCCCCTTGTAGGa                                  <  2:4153682/151‑1 (MQ=17)
                                                                                                                     tACATCAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGgaggag                                 >  1:1567775/1‑151 (MQ=255)
                                                                                                                          cAACCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCaa                                                                >  2:310177/1‑115 (MQ=255)
                                                                                                                            aCCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCAc                                                <  2:3246766/129‑1 (MQ=18)
                                                                                                                            aCCGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCAccgtc                                                     >  2:796585/1‑121 (MQ=255)
                                                                                                                             ccGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACcgccgc                                                                                     <  2:5078481/91‑1 (MQ=255)
                                                                                                                              cGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCAc                        <  1:4867502/151‑1 (MQ=21)
                                                                                                                              cGGTGGTGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCAc                        <  1:4514439/151‑1 (MQ=21)
                                                                                                                               ggtggtGTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCCCCCTTGTAGGAg                                 >  1:1667585/1‑141 (MQ=21)
                                                                                                                                gtggtgTCGCCCCACCCCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCAccc                      <  2:729721/151‑1 (MQ=21)
                                                                                                                                gtggtgTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCAccc                      <  1:3840406/151‑1 (MQ=21)
                                                                                                                                gtggtgTCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCAccgtc                                                     >  2:3864194/1‑117 (MQ=255)
                                                                                                                                 cgacgtCGCCCCGCTCCGCCGTAGGAGCGGGGGCACCCGCGAATAGGCCGGACCTGCCGCCCAATGCCGCCAGTCATTCCCCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCACCCTTGTAGGAGCGGGTTCACCcg                     <  2:2632345/147‑1 (MQ=21)
                                                                                                                                   gtgtCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCACCcgcg                   <  1:4229349/151‑1 (MQ=21)
                                                                                                                                     gtCGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAg                                                                                                   <  2:4713017/69‑1 (MQ=255)
                                                                                                                                       cGCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTCCCGTTGTAGGAGCGGGTTCACCCGCGAATa               >  1:2105780/1‑151 (MQ=21)
                                                                                                                                        gccacgtacCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCCGACCTGCCGCACAAAACCCCCGGGCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCGCCGTCGCCACGCCCCCTTGTAGGAGCGGGTTCACCCGCGAATAg              <  1:3946591/144‑1 (MQ=18)
                                                                                                                                        gCCCCACACCGTCGTCGGAGCAGGTTCACCCGTGAATCGGCCGGACCTGCCGCCCAATGCCTCCAGTCCTTCCCCGCCGCACCCGCCTCCGCATTCATCAATCGGCACCGTCGCCACGTACCGTGGTGGGAGCGGGTTCACCCGCGAATAg              <  2:2716977/151‑1 (MQ=255)
                                                                                                                                        gCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCACCCGCGAATAg              <  2:605627/151‑1 (MQ=21)
                                                                                                                                        gCCCCACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCACCCGCGAATAg              <  2:2802508/151‑1 (MQ=21)
                                                                                                                                         ccacgcacCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCCCGCCCCCTTGTAGCTGCGGGTGCACCCGCGAATAgg             >  1:4437614/6‑151 (MQ=17)
                                                                                                                                          cccACACCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCACCCGCGAATAGGc            <  1:1901763/151‑1 (MQ=21)
                                                                                                                                           acgtacCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCAt                                                                       <  1:4298932/87‑1 (MQ=255)
                                                                                                                                             gtacCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGc                                                   <  2:225690/107‑1 (MQ=255)
                                                                                                                                             acacCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGTACCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCgg         <  1:4718103/151‑1 (MQ=21)
                                                                                                                                              cacCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCTCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGTa                                     >  2:2353183/1‑122 (MQ=255)
                                                                                                                                              cacCGTCGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGTa                                     <  1:2353183/122‑1 (MQ=255)
                                                                                                                                               acCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCAc                                                                                           <  1:3890854/67‑1 (MQ=35)
                                                                                                                                               acCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCCCCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGAc       >  1:3206917/1‑151 (MQ=255)
                                                                                                                                                ccGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATCCCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCCCCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGAcc      <  1:966627/151‑1 (MQ=255)
                                                                                                                                                 cGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATGCCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCCCCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCt     <  1:2029398/151‑1 (MQ=255)
                                                                                                                                                   ttGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGTAGGAGCGCGTTCACCCGCGAATAGGCCAGACCTgc   >  2:685241/1‑151 (MQ=255)
                                                                                                                                                    tGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCa                                                       >  2:4320004/1‑98 (MQ=255)
                                                                                                                                                    tGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCCGCCACCGCATTCATCAATCGGCACCGTCGCCACGCCCCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTgcc  <  1:4085778/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GGTGCGCGGGATGACAGCGTGGCGATGCAGTTCCTGATTCCGGGTTGGAGCTTCGATAACAAACGGCCTTGCCTGGTGCGTTGAGGCTGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACATCAACCGGTGGTGTCGCCCCACACCGTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCCGCCCAATACCCCCAGTCATTCACCGCCGCACCTGCCACCGCATTCATCAATCGGCACCATCGCCACGCACCCTTGTAGGAGCGGGTTCACCCGCGAATAGGCCGGACCTGCC  >  CP009974/2910013‑2910311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: