Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,982,223 T→G intergenic (‑302/+644) RPPX_13295 ← / ← RPPX_13300 integrase/integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,982,2230TG99.6% 969.3 / NA 273intergenic (‑302/+644)RPPX_13295/RPPX_13300integrase/integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (124/148);  minor base A (1/0);  total (125/148)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ATGATCTGGCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTGCGGCACCATCATCTACATCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGA  >  CP009974/2982075‑2982369
                                                                                                                                                    |                                                                                                                                                  
aTGATCTGGCGTCCGGAACACGGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTg                                                                                                                                                  <  1:4476599/151‑1 (MQ=255)
aTGATCTGGCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTg                                                                                                                                                  >  2:5218840/1‑151 (MQ=255)
  gATGTGGCCGCCTGAAAACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGt                                                                                                                                                <  2:107915/151‑1 (MQ=255)
   gtCTGGCCTCCTGAACGCCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTc                                                                                                                                               <  2:280357/150‑1 (MQ=255)
   aTCTGGCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTc                                                                                                                                               >  2:2875903/1‑151 (MQ=255)
    tCTGGCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATCGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCt                                                                                                                                              <  2:5061000/151‑1 (MQ=255)
     cTGGCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCACTGCGATGACCGGGGCCTGCCTGACTGTCAGTCACGTGCAGTCCACTCCGTGGTCTg                                                                                                                                             >  2:4172276/1‑151 (MQ=255)
        gCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGCCTGCgg                                                                                                                                          >  2:4378946/1‑151 (MQ=255)
           tcctGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCAc                                                                                                                                       >  2:334697/1‑151 (MQ=255)
             ctGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACca                                                                                                                                     >  1:438669/1‑151 (MQ=255)
              tGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACcat                                                                                                                                    >  2:4321285/1‑151 (MQ=255)
               gAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACcatc                                                                                                                                   <  1:2635953/151‑1 (MQ=255)
                aaCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACcatca                                                                                                                                  <  2:1797021/151‑1 (MQ=255)
                  cacCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCatcatc                                                                                                                                <  1:713520/151‑1 (MQ=255)
                  cacCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCatcatc                                                                                                                                <  2:4474265/151‑1 (MQ=255)
                    ccGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTa                                                                                                                              <  2:1278963/151‑1 (MQ=255)
                     cGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTAc                                                                                                                             <  1:3668692/151‑1 (MQ=255)
                       ttACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACag                                                                                                                           <  2:1321713/151‑2 (MQ=255)
                        tACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACagc                                                                                                                          <  2:3705311/151‑3 (MQ=255)
                         aCCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCTAGCAGCAATGAGATGACCGCGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCg                                                                                                                         <  2:3254389/151‑1 (MQ=255)
                             gTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGt                                                                                                                     <  1:2920287/151‑1 (MQ=255)
                              tGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGtt                                                                                                                    <  2:4697112/151‑1 (MQ=255)
                               gggCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTg                                                                                                                   <  1:3773502/151‑1 (MQ=255)
                               gggCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTg                                                                                                                   <  2:3611706/151‑1 (MQ=255)
                                   gggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCTCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTgg                                                                                                               >  1:333284/1‑151 (MQ=255)
                                   gggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTgg                                                                                                               >  2:857451/1‑151 (MQ=255)
                                    ggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCCTCGTCTACAGCGCGGTTGCTGGt                                                                                                              >  2:3755629/1‑151 (MQ=255)
                                    ggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGt                                                                                                              >  2:5186309/1‑151 (MQ=255)
                                    ggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGCGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGt                                                                                                              >  2:580758/1‑151 (MQ=255)
                                     ggggAGGCTGCAGTGGCTGAGGGGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATGATCTACAGCGCTGTTGCTGGTg                                                                                                             <  2:4660492/149‑1 (MQ=14)
                                     gTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTg                                                                                                             >  1:277756/1‑151 (MQ=255)
                                       ggAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGAc                                                                                                           <  2:3886351/151‑1 (MQ=255)
                                          ggCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATc                                                                                                        >  2:3053056/1‑151 (MQ=255)
                                           gCTGCATTGGCTGACGAGACCAGTGCCGCGCGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCg                                                                                                       <  2:142948/151‑1 (MQ=255)
                                            cTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCgg                                                                                                      >  1:679468/1‑151 (MQ=255)
                                                 ttGGCTGACGAGACCGGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGttt                                                                                                 <  1:881856/151‑1 (MQ=255)
                                                     cTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCa                                                                                             >  1:2496041/1‑151 (MQ=255)
                                                      tGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCAc                                                                                            >  2:1967953/1‑151 (MQ=255)
                                                       gACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACg                                                                                           >  1:3598036/1‑151 (MQ=255)
                                                       gACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACg                                                                                           <  1:5227308/151‑1 (MQ=255)
                                                          gagaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCg                                                                                        >  2:4605334/1‑151 (MQ=255)
                                                           agaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGa                                                                                       >  1:3150145/1‑151 (MQ=255)
                                                             tccAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAtt                                                                                     >  2:2202701/2‑151 (MQ=255)
                                                             aCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAtt                                                                                     >  2:1903130/1‑151 (MQ=255)
                                                             aCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAtt                                                                                     <  1:2342952/151‑1 (MQ=255)
                                                                   gCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACg                                                                               <  1:4954315/151‑1 (MQ=255)
                                                                        gagaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgggg                                                                          <  2:659619/151‑1 (MQ=255)
                                                                          gaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGa                                                                        <  2:2011518/151‑1 (MQ=255)
                                                                           aTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGaa                                                                       <  2:4947843/151‑1 (MQ=255)
                                                                             ccTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcg                                                                     >  1:2307643/1‑151 (MQ=255)
                                                                              cTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgc                                                                    <  1:4708295/151‑1 (MQ=255)
                                                                              cTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgc                                                                    >  2:4260313/1‑151 (MQ=255)
                                                                                 aGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTGCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgccgc                                                                 <  2:879765/151‑1 (MQ=255)
                                                                                   ccATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCaa                                                               >  1:84099/1‑151 (MQ=255)
                                                                                    cATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCaaa                                                              <  1:1447748/151‑1 (MQ=255)
                                                                                    cATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCATGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGCGGGAACGCCGCaaa                                                              >  2:4853649/1‑151 (MQ=255)
                                                                                      tgtgCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGc                                                            <  1:4358248/151‑1 (MQ=255)
                                                                                        tgCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCa                                                          >  1:3322075/1‑151 (MQ=255)
                                                                                          cAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCAtg                                                        >  1:4130566/1‑151 (MQ=255)
                                                                                           aaGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCAtgt                                                       >  2:4877730/1‑151 (MQ=255)
                                                                                             gcagcaATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGc                                                     <  1:499126/151‑1 (MQ=255)
                                                                                              cagcaATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGCCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCg                                                    >  2:646531/1‑151 (MQ=255)
                                                                                                     gCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAggg                                             >  1:582984/1‑151 (MQ=255)
                                                                                                      cGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGc                                            <  1:4260313/151‑1 (MQ=255)
                                                                                                       gATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCt                                           >  1:1131333/1‑151 (MQ=255)
                                                                                                       gATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCt                                           <  1:194357/151‑1 (MQ=255)
                                                                                                          gACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgca                                        <  1:2067510/151‑1 (MQ=255)
                                                                                                              ggggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGcagcag                                    <  1:1864016/151‑1 (MQ=255)
                                                                                                               gggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGcagcagg                                   >  2:2426020/1‑150 (MQ=255)
                                                                                                               gggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGt                                   >  1:3221779/1‑151 (MQ=255)
                                                                                                                ggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCaaa                                                              <  1:3401505/123‑1 (MQ=255)
                                                                                                                ggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTg                                  <  1:1284922/151‑1 (MQ=255)
                                                                                                                ggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTg                                  <  2:1733486/151‑1 (MQ=255)
                                                                                                                 gCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTgg                                 >  2:635548/1‑151 (MQ=255)
                                                                                                                  cATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGt                                <  1:2414470/151‑1 (MQ=255)
                                                                                                                   aTGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTa                               >  2:3581453/1‑151 (MQ=255)
                                                                                                                   aTGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTa                               <  2:2475332/151‑1 (MQ=255)
                                                                                                                    tGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAg                              >  1:2748849/1‑151 (MQ=255)
                                                                                                                      cgtGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGaa                            <  2:2915340/149‑1 (MQ=255)
                                                                                                                      ccTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGaa                            <  2:3433846/151‑1 (MQ=255)
                                                                                                                      ccTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGaa                            >  1:4007848/1‑151 (MQ=255)
                                                                                                                      ccTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGaa                            <  1:4829341/151‑1 (MQ=255)
                                                                                                                       cTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAg                           >  1:1982786/1‑151 (MQ=255)
                                                                                                                        tGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGt                          >  1:3469336/1‑151 (MQ=255)
                                                                                                                        tGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGt                          >  1:2605220/1‑151 (MQ=255)
                                                                                                                         gACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTg                         >  1:1015199/1‑151 (MQ=255)
                                                                                                                         gACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTg                         >  1:4090002/1‑151 (MQ=255)
                                                                                                                            tGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCACTGTTGCTGGTGACAACGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGccc                      <  1:1537266/151‑1 (MQ=255)
                                                                                                                             gtcagtcaGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCg                     <  1:1686087/151‑1 (MQ=255)
                                                                                                                              tcagtcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGt                    <  1:3714407/151‑1 (MQ=255)
                                                                                                                               cagtcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGtc                   <  1:3563721/151‑1 (MQ=255)
                                                                                                                                aggcagGTGCTGTCCATTCCGTGGTCTGCGGCCCCATCATCTACAGCGCTGTTGCTGGTGACATCCGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGtct                  <  2:4061435/148‑1 (MQ=14)
                                                                                                                                 gtcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGtctc                 <  2:2123906/151‑1 (MQ=255)
                                                                                                                                  tcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTctct                <  2:3395573/151‑1 (MQ=255)
                                                                                                                                         cAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaag         <  1:2832165/151‑1 (MQ=255)
                                                                                                                                          aGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaaga        <  1:3879400/151‑1 (MQ=255)
                                                                                                                                            tCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagaga      >  2:2744110/1‑151 (MQ=255)
                                                                                                                                              cATTCCGTGGTCTGCGGCACCATCCTCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCGGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaa    <  1:5066515/151‑1 (MQ=255)
                                                                                                                                               aTTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaag   <  2:1111145/151‑1 (MQ=255)
                                                                                                                                                ttCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaaga  <  2:3598036/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATGATCTGGCCTCCTGAACACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTGCGGCACCATCATCTACATCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGA  >  CP009974/2982075‑2982369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑