Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,982,248 T→G intergenic (‑327/+619) RPPX_13295 ← / ← RPPX_13300 integrase/integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,982,2480TG99.6% 967.4 / ‑6.0 277intergenic (‑327/+619)RPPX_13295/RPPX_13300integrase/integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (128/148);  minor base C (1/0);  total (129/148)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 277 total aligned reads displayed.

ACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTGCGGCACCATCATCTACATCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATATT  >  CP009974/2982100‑2982394
                                                                                                                                                    |                                                                                                                                                  
  cGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCt                                                                                                                                                >  1:4240605/1‑151 (MQ=255)
     tGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGtt                                                                                                                                             <  2:4697112/151‑1 (MQ=255)
        gCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCt                                                                                                                                          <  2:5225273/151‑1 (MQ=255)
          gggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTgg                                                                                                                                        >  1:334489/1‑151 (MQ=255)
           ggTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCCTCGTCTACAGCGCGGTTGCTGGt                                                                                                                                       >  2:3755629/1‑151 (MQ=255)
            ggggAGGCTGCAGTGGCTGAGGGGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATGATCTACAGCGCTGTTGCTGGTg                                                                                                                                      <  2:4660492/149‑1 (MQ=14)
            gTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTg                                                                                                                                      >  1:277676/1‑151 (MQ=255)
              ggAGGCTGGAGTGGCTGACGAGGCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCGGACTGGCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGAc                                                                                                                                    <  1:5145991/151‑1 (MQ=14)
              ggAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGAc                                                                                                                                    <  1:4365327/151‑1 (MQ=255)
              ggAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGAc                                                                                                                                    <  2:2111904/151‑1 (MQ=255)
               gAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCGATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACa                                                                                                                                   <  1:2676312/151‑1 (MQ=255)
               gAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACa                                                                                                                                   <  2:306184/151‑1 (MQ=255)
                    tGCAGTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGc                                                                                                                              <  2:2377248/151‑1 (MQ=255)
                       aTTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGtt                                                                                                                           >  1:2468315/1‑151 (MQ=255)
                             tGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCAc                                                                                                                     >  2:1967953/1‑151 (MQ=255)
                             agACGAGACCAGTGCCGGGAGATCCTGAGCGATGTGCAAGCAGCAATGGGATGGCCGGGGCACGCCGGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCAGCTAAAGCGCCGGTGCAGGTGACATCGGCGTTTGacac                                                                                                                     <  2:2758296/150‑1 (MQ=14)
                               aCGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGc                                                                                                                   >  1:3001857/1‑151 (MQ=255)
                                 gagaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTc                                                                                                                       >  2:4367275/1‑145 (MQ=255)
                                  agaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGa                                                                                                                >  1:3150589/1‑151 (MQ=255)
                                   gaCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAt                                                                                                               >  1:3817264/1‑151 (MQ=255)
                                    aCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAtt                                                                                                              >  2:1903130/1‑151 (MQ=255)
                                    aCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAtt                                                                                                              >  1:64860/1‑151 (MQ=255)
                                    aCCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACACCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGAtt                                                                                                              >  1:3603524/1‑151 (MQ=255)
                                          gCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACg                                                                                                        <  1:4954315/151‑1 (MQ=255)
                                             gcgAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgg                                                                                                     <  2:3594666/151‑1 (MQ=255)
                                             gcgAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAgg                                                                                                     >  2:648277/1‑151 (MQ=255)
                                              cgAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAggg                                                                                                    <  2:3986375/151‑1 (MQ=255)
                                              cgAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAggg                                                                                                    >  1:4034120/1‑151 (MQ=255)
                                                 gaTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGa                                                                                                 <  2:2011518/151‑1 (MQ=255)
                                                  aTCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGaa                                                                                                <  2:4947843/151‑1 (MQ=255)
                                                  aTCCAGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGaa                                                                                                <  2:708221/151‑1 (MQ=255)
                                                   tCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAc                                                                                               <  2:330514/151‑1 (MQ=255)
                                                     cTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgc                                                                                             >  2:4260313/1‑151 (MQ=255)
                                                        aGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTGCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAAcgccgc                                                                                          <  2:879765/151‑1 (MQ=255)
                                                         gCCATGTGCAAGCAGCCATGCGCTGACCGGGCCATGCCTCACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCa                                                                                         <  2:1498158/151‑1 (MQ=14)
                                                          ccATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCaa                                                                                        >  1:84099/1‑151 (MQ=255)
                                                           cATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCATGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGCGGGAACGCCGCaaa                                                                                       >  2:4853649/1‑151 (MQ=255)
                                                            aTGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAg                                                                                      <  2:3616333/151‑1 (MQ=255)
                                                               tgCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCa                                                                                   >  1:3322075/1‑151 (MQ=255)
                                                                 cAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCAtg                                                                                 >  1:4130566/1‑151 (MQ=255)
                                                                  aaGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCAtgt                                                                                <  1:3437433/151‑1 (MQ=255)
                                                                    gcagcaATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGc                                                                              <  2:1774375/151‑1 (MQ=255)
                                                                    gcagcaATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGc                                                                              >  1:5059394/1‑151 (MQ=255)
                                                                          aTGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAg                                                                        >  2:1109752/1‑151 (MQ=255)
                                                                           tGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAgg                                                                       <  1:4347184/151‑1 (MQ=255)
                                                                            gCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAggg                                                                      >  1:582984/1‑151 (MQ=255)
                                                                              gATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCt                                                                    >  1:1131333/1‑151 (MQ=255)
                                                                              gATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCt                                                                    <  1:194357/151‑1 (MQ=255)
                                                                               aTGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTg                                                                   <  2:2144944/151‑1 (MQ=255)
                                                                                 gACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgca                                                                 <  1:2067510/151‑1 (MQ=255)
                                                                                   ccGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTgcagc                                                               <  1:3575304/151‑1 (MQ=255)
                                                                                     ggggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGcagcag                                                             >  1:3897230/1‑151 (MQ=255)
                                                                                     ggggCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGcagcag                                                             <  1:1864016/151‑1 (MQ=255)
                                                                                       ggCACGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAACGCCATGTGCGATCAGGGCTGCAGCAGTg                                                           >  2:4301896/1‑151 (MQ=255)
                                                                                        gCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTgg                                                          >  2:635474/1‑151 (MQ=255)
                                                                                        gCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTgg                                                          >  2:636686/1‑151 (MQ=255)
                                                                                         cATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGt                                                         <  1:2809780/151‑1 (MQ=255)
                                                                                         cATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGt                                                         <  1:2414470/151‑1 (MQ=255)
                                                                                          aTGCCTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTa                                                        <  2:2475332/151‑1 (MQ=255)
                                                                                            gCCTGACTGTCAGTCAGGTGCAGTCCATTCCGGGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGa                                                      <  2:5078019/151‑1 (MQ=255)
                                                                                             ccTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGaa                                                     <  1:4829341/151‑1 (MQ=255)
                                                                                             ccTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGaa                                                     <  2:3433846/151‑1 (MQ=255)
                                                                                              cTGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAg                                                    >  1:1982786/1‑151 (MQ=255)
                                                                                               tGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGt                                                   <  2:1423007/151‑1 (MQ=255)
                                                                                               tGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGt                                                   >  2:3476109/1‑151 (MQ=255)
                                                                                               tGACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGt                                                   >  1:2605220/1‑151 (MQ=255)
                                                                                                gACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTg                                                  >  1:4090002/1‑151 (MQ=255)
                                                                                                gACTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTg                                                  >  1:1015199/1‑151 (MQ=255)
                                                                                                 aCTGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGc                                                 <  1:4605334/151‑1 (MQ=255)
                                                                                                   tGTCAGTCAGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGccc                                               >  1:1477933/1‑151 (MQ=255)
                                                                                                    gtcagtcaGGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCg                                              <  1:1686087/151‑1 (MQ=255)
                                                                                                     tcagtcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGt                                             <  1:3714407/151‑1 (MQ=255)
                                                                                                       aggcagGTGCTGTCCATTCCGTGGTCTGCGGCCCCATCATCTACAGCGCTGTTGCTGGTGACATCCGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGtct                                           <  2:4061435/148‑1 (MQ=14)
                                                                                                         tcagGTGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTctct                                         <  2:3395573/151‑1 (MQ=255)
                                                                                                            ggTGCAGTCCATTCCGTGGTCTGCGGCCCCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTCTCACGCCGATTGTCACGAGGGGGCACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTc                                      <  2:2828576/151‑1 (MQ=255)
                                                                                                              tGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCa                                    >  1:682290/1‑151 (MQ=255)
                                                                                                              tGCAGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCa                                    >  2:4553397/1‑151 (MQ=255)
                                                                                                                 aGTCCATTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaaga                                 <  1:2318966/151‑1 (MQ=255)
                                                                                                                      aTTCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaag                            <  1:4957820/151‑1 (MQ=255)
                                                                                                                       ttCCGTGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaaga                           <  1:1340945/151‑1 (MQ=255)
                                                                                                                         ccgtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaagaga                         <  2:4877987/148‑1 (MQ=255)
                                                                                                                         ccgtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCaagagaaagaga                         >  2:5131393/4‑151 (MQ=255)
                                                                                                                          cgtGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGAc                        <  1:1233553/149‑1 (MQ=255)
                                                                                                                            tGGTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACgg                      <  2:679468/151‑1 (MQ=255)
                                                                                                                              gTCTGCGGCACGATCGTCTGCAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGc                    <  1:4717945/151‑1 (MQ=255)
                                                                                                                              gTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGc                    >  2:4227980/1‑151 (MQ=255)
                                                                                                                              gTCTGCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGc                    >  2:26439/1‑151 (MQ=255)
                                                                                                                                  gcgtgcgcatcagCGACAGGGCTGGTGCTGGTCACGTCGGCGTTTGACACGCCGATTGTGACGCGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTTGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAg                <  2:4777524/144‑1 (MQ=17)
                                                                                                                                  gCGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAg                <  2:1083938/151‑1 (MQ=255)
                                                                                                                                   cGGCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAgg               <  1:2756790/151‑1 (MQ=255)
                                                                                                                                     gCACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCGGATTGTCACGAGGTGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGtg             <  2:2906621/151‑2 (MQ=255)
                                                                                                                                      cACCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTg            >  1:4501361/1‑151 (MQ=255)
                                                                                                                                       aCCATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTgg           <  1:2202701/151‑1 (MQ=255)
                                                                                                                                        ccATCATCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGgc          >  1:352616/1‑151 (MQ=255)
                                                                                                                                         catcatCTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGgcg         <  1:3188247/151‑1 (MQ=255)
                                                                                                                                           tcatcTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCa       <  1:1541330/151‑1 (MQ=255)
                                                                                                                                            catcTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCaa      <  1:3242297/151‑1 (MQ=255)
                                                                                                                                             atcTACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAat     <  2:2928622/151‑1 (MQ=255)
                                                                                                                                               cTACAGAGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAatat   <  2:3048805/151‑1 (MQ=255)
                                                                                                                                                tACAGCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATAtt  >  2:1001046/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCATGTGCAAGCAGCAATGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTGCGGCACCATCATCTACATCGCTGTTGCTGGTGACATCGGCGTTTGTCACGCCGATTGTCACGAGGGGGAACGCCGCAAAGCCATGTGCGATCAGGGCTGCAGCAGTGGTAGAAGTGCCCGTCTCTTTCCAAGAGAAAGAGACGGGCACAGGTTGGCGCAATATT  >  CP009974/2982100‑2982394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑