Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,037,787 C→T G223D (GGC→GAC)  RPPX_13530 ← Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,037,7870CT96.6% 905.6 / 3.0 269G223D (GGC→GAC) RPPX_13530Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base T (134/124);  total (142/127)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

CTCGCCTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGCCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGCACATTGG  >  CP009974/3037639‑3037934
                                                                                                                                                    |                                                                                                                                                   
ctcGCCTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGGACGCGCCTTTTTCGCGGTCg                                                                                                                                                   >  2:5152883/1‑151 (MQ=255)
ctcGCCTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCg                                                                                                                                                   >  2:2464281/1‑151 (MQ=255)
  cGCCTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGaa                                                                                                                                                 >  1:142216/1‑151 (MQ=255)
   gCCTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAAc                                                                                                                                                >  1:3635052/1‑151 (MQ=255)
     cTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCACCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAg                                                                                                                                              <  1:834853/151‑1 (MQ=255)
      ttCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCTGCGGCCTCCCCCAAGCCGGCGCGCGCGCCGCTGGCCCCGGTGACCGCGCCTTTTGCGTGCTCGAACAGt                                                                                                                                             >  2:10387/1‑151 (MQ=255)
      ttCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGt                                                                                                                                             <  1:1738003/151‑1 (MQ=255)
       tCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGtt                                                                                                                                            >  1:2721436/1‑151 (MQ=255)
       tCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGtt                                                                                                                                            <  2:3577306/151‑1 (MQ=255)
        ccTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTc                                                                                                                                           <  1:5170918/151‑1 (MQ=255)
         cTGCAGCACACGCAGCAGTCGAGCGGGGGGTTACAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCg                                                                                                                                          <  2:330351/151‑1 (MQ=255)
         cTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCg                                                                                                                                          >  2:1484266/1‑151 (MQ=255)
           gcagcaCACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTTGAACCGTTCGGa                                                                                                                                        >  1:4712025/1‑151 (MQ=255)
           gcagcaCACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGa                                                                                                                                        >  1:2086018/1‑151 (MQ=255)
              gcaCACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTc                                                                                                                                     <  2:853337/151‑1 (MQ=255)
               cacaCGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCCAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAGCCGTTCGGATTCg                                                                                                                                    >  2:1842261/1‑151 (MQ=255)
                acacGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTGTTGGTCGCACAGTTCgggtgacc                                                                                                                                   >  2:4215667/1‑145 (MQ=255)
                acacGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGa                                                                                                                                   <  1:601174/151‑1 (MQ=255)
                 cacGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGAt                                                                                                                                  <  2:734766/151‑1 (MQ=255)
                    gcagcaGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAg                                                                                                                               >  1:1155481/1‑151 (MQ=255)
                     cagcagTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGc                                                                                                                              <  1:525243/151‑1 (MQ=255)
                      agcagTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCg                                                                                                                             >  2:2255313/1‑151 (MQ=255)
                        cagTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGtt                                                                                                                           >  1:3895294/1‑151 (MQ=255)
                        cagTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGtt                                                                                                                           >  2:4442372/1‑151 (MQ=255)
                         agTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGCACAGTTCGGATTCGATCAGCGttt                                                                                                                          >  1:504510/1‑151 (MQ=255)
                            cgcgCCTGTGCTGGCAGCGGCGGGGCGCCGATTTCACCGAGGCACAGCGTACCGGCGTCGTCGGGCTCGACCACGCCGGCGCGCCCGGCGGTGGCGCCGGTGAACGCGCCTTTTTCGGGGTCGAACAGTGCGGATTCGATCAGCGTTTCgg                                                                                                                       <  2:3705643/148‑1 (MQ=255)
                            cGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCgg                                                                                                                       <  1:1627293/151‑1 (MQ=255)
                            cGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTGCgg                                                                                                                       >  2:68933/1‑151 (MQ=255)
                             gAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTCGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCggg                                                                                                                      >  2:4989268/1‑151 (MQ=255)
                                ccTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGAt                                                                                                                   >  1:1554627/1‑151 (MQ=255)
                                 cTGGGCTTCGCGCGGCAGGTCGCCAATGTCCTCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATg                                                                                                                  <  2:4244916/151‑1 (MQ=255)
                                      cTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCcgc                                                                                                             <  2:1022439/151‑1 (MQ=255)
                                           aGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCCCCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGCCGAACCGCTCGGCTTCGCTCAGCGCTCCGGGGACGGCCgcgccgc                                                                                                        >  2:4649838/1‑148 (MQ=255)
                                            gctgCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGtt                                                                                                       >  1:1045868/4‑151 (MQ=255)
                                              ggCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCa                                                                                                     <  2:1647402/151‑1 (MQ=255)
                                              ggCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCa                                                                                                     <  1:593128/151‑1 (MQ=255)
                                              ggCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCa                                                                                                     <  2:2079264/151‑1 (MQ=255)
                                                 aGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCg                                                                                                  >  2:420138/1‑151 (MQ=255)
                                                    tCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCgaga                                                                                               >  2:4793288/1‑151 (MQ=255)
                                                      gCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGCGatc                                                                                             >  2:2828232/1‑151 (MQ=255)
                                                       ccAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGatca                                                                                            >  1:4776318/1‑151 (MQ=255)
                                                        naaTTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGatcat                                                                                           >  1:4432752/2‑151 (MQ=255)
                                                           tttCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCgg                                                                                        >  1:770133/1‑151 (MQ=255)
                                                            ttCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGt                                                                                       <  1:4572149/151‑1 (MQ=255)
                                                               aTCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGcc                                                                                    >  2:2765705/1‑151 (MQ=255)
                                                               aTCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGcc                                                                                    <  1:2617626/151‑1 (MQ=255)
                                                                      aaCAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGccc                                                                             <  1:2325760/151‑1 (MQ=255)
                                                                        cAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCgg                                                                           >  1:4602674/1‑151 (MQ=255)
                                                                         aGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGc                                                                          <  2:3790801/151‑1 (MQ=255)
                                                                         aGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGc                                                                          >  2:2278683/1‑151 (MQ=255)
                                                                          gCGTACCGCCATCGGCGGCCTCCACCAGGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCg                                                                         <  2:1901178/151‑1 (MQ=255)
                                                                          gCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCg                                                                         <  2:472815/151‑1 (MQ=255)
                                                                            gTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATGAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCgag                                                                       >  2:3002014/1‑151 (MQ=255)
                                                                            gTACCGCCATCGCCCGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCgag                                                                       <  1:1996710/151‑1 (MQ=255)
                                                                             tACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCAGGCgcga                                                                      >  2:3205696/1‑151 (MQ=255)
                                                                              gccGCCCTCGGCGGCCGCCACCAGGCCGGCCCGCCCGGCGCTCGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTGTCCGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGAc                                                                     <  1:2532966/150‑1 (MQ=255)
                                                                               ccGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACa                                                                    >  1:4864026/1‑151 (MQ=255)
                                                                                  ccATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGt                                                                 >  1:5094499/1‑151 (MQ=255)
                                                                                  ccATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTGTTCCTGGGCGAGGAGTTCGGGTTCGATCAGCGCTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCGGGCGAGACAgtc                                                                 >  2:4545391/1‑149 (MQ=255)
                                                                                   cATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCCGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGCGACCGGtt                                                                >  2:4584237/1‑151 (MQ=255)
                                                                                    aTCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTtg                                                               >  1:1685486/1‑151 (MQ=255)
                                                                                      ggggggcCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTGTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTtgtg                                                             <  2:2623268/147‑1 (MQ=255)
                                                                                       ggcggcCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGATGTGc                                                            >  1:657410/1‑151 (MQ=255)
                                                                                         cggcCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAg                                                          >  1:1745854/1‑151 (MQ=255)
                                                                                          ggcCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTTGTTTTTGTTCAGTTAGGATGAGGTCAGCGTAGCGGGCATGGCCGCGCAGTGCGCCGAGGTCCGCCGCGCCGTGGGCCGGCGAGACAGGGGGTGCggg                                                         >  2:1409875/1‑151 (MQ=255)
                                                                                          ggcCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAgg                                                         <  1:1484266/151‑1 (MQ=255)
                                                                                          ggcCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGATTCGGGGCTGGCCGCGCAGTTCACCGAGACCATCGGGGCCCCGGCCCGGCGAGCCAggtggtcccgg                                                         >  1:2965166/1‑146 (MQ=255)
                                                                                                  ccaAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGc                                                 <  1:2643008/151‑1 (MQ=255)
                                                                                                  ccaAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGc                                                 >  1:92876/1‑151 (MQ=255)
                                                                                                        cGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAg                                           <  1:1865401/151‑1 (MQ=255)
                                                                                                        cGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAg                                           >  1:4235064/1‑151 (MQ=255)
                                                                                                        cGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCAGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAg                                           <  1:4508137/151‑1 (MQ=255)
                                                                                                          gcgcgcCCGGCGCTGGCGCCGGTGAACGCGGCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGct                                         <  2:3492300/151‑1 (MQ=255)
                                                                                                                ccGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTAc                                   <  2:563162/151‑1 (MQ=255)
                                                                                                                ccGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTAc                                   <  1:3121535/151‑1 (MQ=255)
                                                                                                                ccGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCACGGCGGGACAGGTTGTGGAGGGCGCGGGCGACCAGGTCTTTAc                                   >  2:2891106/1‑151 (MQ=255)
                                                                                                                  ggCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCg                                 <  1:2278683/151‑1 (MQ=255)
                                                                                                                    cgcTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGt                               <  1:3665861/151‑1 (MQ=255)
                                                                                                                     gcTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCCTCGGTGCCTTGGCCCGGCGGGACGGGTTGTGCAGGGGGCGGGCCACCAGCTCTTTCCCgggg                              >  2:3898726/1‑149 (MQ=255)
                                                                                                                     gcTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGtc                              >  2:3410269/1‑150 (MQ=255)
                                                                                                                      cTGGCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGc                             <  2:1389563/151‑1 (MQ=255)
                                                                                                                        ggCGCCGGTGAAGGCGCGTTTTTCGTGGTCGAACAGTTCGGATTCGAGCAGCGTTTCGGGGATGGCCGCGCAGTGCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGccc                           <  2:490970/151‑1 (MQ=255)
                                                                                                                        ggCGCCGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGccc                           <  1:5009934/151‑1 (MQ=255)
                                                                                                                         gcgcCGGTGAACGCGCCTTTTTCTTTGTTTAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCGGGGCGCGGGCCACCAGCTCGTTACCGGTGCCCg                          >  2:505742/1‑151 (MQ=255)
                                                                                                                            ccGGTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACt                       >  1:5102569/1‑151 (MQ=255)
                                                                                                                              ggTGAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCg                     >  1:1289040/1‑151 (MQ=255)
                                                                                                                               gTGAACGCGCCTTTTTCGTGGCCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGc                    >  2:1241956/1‑151 (MQ=255)
                                                                                                                                 gAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGccc                  >  1:3483971/1‑151 (MQ=255)
                                                                                                                                 gAACGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCCCTTTACCGGTGCCCGACTCGccc                  >  2:4768322/1‑151 (MQ=255)
                                                                                                                                   aCGCGCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTg                >  2:408537/1‑151 (MQ=255)
                                                                                                                                    cgcgCCTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTgg               <  1:2447164/151‑1 (MQ=255)
                                                                                                                                        ccTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGGTTCGGGGATGGCCGCGCAGTTCACCGAGACCATCGGTGCCGTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGTGGCACCAGCTCCTTACCGGTTCCCGTCTCGCCCTGCATcc           >  2:4521716/1‑150 (MQ=255)
                                                                                                                                         cTTTTTCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAg          <  1:3178382/151‑1 (MQ=255)
                                                                                                                                          tttttCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGc         >  1:409498/1‑151 (MQ=255)
                                                                                                                                           ttttCGTGGTCGAACAGTTGGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCCCCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGca        <  1:1466127/151‑1 (MQ=255)
                                                                                                                                            tttCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGcac       <  2:637636/151‑1 (MQ=255)
                                                                                                                                             ttCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGcacn      <  1:4954840/151‑2 (MQ=255)
                                                                                                                                              tCGTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGCACAt     <  1:3580340/151‑1 (MQ=255)
                                                                                                                                                gTGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCCTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGCACATTg   <  2:32243/151‑1 (MQ=255)
                                                                                                                                                 tGGTCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGCACATTgg  >  2:2320148/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCGCCTTCCTGCAGCACACGCAGCAGTCGAGCCTGGGCTTCCAGCGGCAGTTCGCCAATTTCATCGAGGAACAGCGTACCGCCATCGGCGGCCTCCACCAAGCCGGCGCGCCCGGCGCTGGCGCCGGTGAACGCGCCTTTTTCGTGGCCGAACAGTTCGGATTCGATCAGCGTTTCGGGGATGGCCGCGCAGTTCACCGAGATCATCGGTGCCTTGGCCCGGCGAGACAGGTTGTGCAGGGCGCGGGCCACCAGCTCTTTACCGGTGCCCGACTCGCCCTGGATCAGCACATTGG  >  CP009974/3037639‑3037934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑