Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,161,709 T→C intergenic (+115/+136) RPPX_14090 → / ← RPPX_14095 RND transporter/short‑chain dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,161,7090TC97.1% 739.3 / 7.1 246intergenic (+115/+136)RPPX_14090/RPPX_14095RND transporter/short‑chain dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (4/3);  new base C (115/123);  total (119/127)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 249 total aligned reads displayed.

GTTTGATGAGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAATCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCACTCAGAGCGGGCG  >  CP009974/3161561‑3161856
                                                                                                                                                    |                                                                                                                                                   
gTTTGATGAGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCa                                                                                                                                                   <  1:1435390/151‑1 (MQ=255)
gTTTGATGAGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCa                                                                                                                                                   >  1:3735773/1‑151 (MQ=255)
  ttGATGAGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAaccacc                                                                                                                                                 >  1:2352152/1‑151 (MQ=255)
   tgatgaGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTACACCCGCTCCTACACGACGGCCTGCGCCCAACCCCCg                                                                                                                                                >  2:4601231/1‑151 (MQ=255)
    gatgaGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGa                                                                                                                                               <  2:158422/151‑1 (MQ=255)
     atgaGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCCGGTAAACCCGCTCCTACACGACGGCCTGCGCCCACCCACCGAt                                                                                                                                              >  2:1056206/1‑151 (MQ=255)
            gcCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTc                                                                                                                                       <  1:254825/151‑1 (MQ=255)
             ccGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCCGCCCCCAACCACCGAtgtgtcgcc                                                                                                                                      >  1:3014401/1‑147 (MQ=255)
                cAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTccacc                                                                                                                                   <  2:4651170/151‑1 (MQ=255)
                 aGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTccacca                                                                                                                                  >  2:2314216/1‑151 (MQ=255)
                   aaTGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGCCCCGCGCCCAACCACCGATGTGTGTccaccacc                                                                                                                                >  2:2086135/1‑151 (MQ=255)
                     tGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCAc                                                                                                                                                  >  2:2067802/1‑131 (MQ=255)
                      gTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTACACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAAt                                                                                                                             >  2:2666955/1‑151 (MQ=255)
                       ttCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCaagc                                                                                                                            >  2:4582807/1‑149 (MQ=255)
                        tCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCt                                                                                                                           <  2:4514616/151‑1 (MQ=255)
                        tCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCGCCTACACGACGGCCTGCGCCTAACCCCCGATGTGTGTCCACCACCAATCt                                                                                                                           >  1:3095860/1‑151 (MQ=255)
                         cacaGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTg                                                                                                                          >  1:64062/1‑151 (MQ=255)
                          acaGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTgg                                                                                                                         <  1:1979612/151‑1 (MQ=255)
                          acaGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTgg                                                                                                                         <  2:4260572/151‑1 (MQ=255)
                            aGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGgtg                                                                                                                       >  1:3249272/1‑151 (MQ=255)
                              ttggggagGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTa                                                                                                                     <  2:626220/146‑1 (MQ=255)
                                agagagGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAgg                                                                                                                   <  1:2269757/151‑1 (MQ=255)
                                 gagagGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGa                                                                                                                  <  1:736495/151‑1 (MQ=255)
                                   gagGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGc                                                                                                                >  1:2140860/1‑151 (MQ=255)
                                    agGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTggggggg                                                                                                               >  2:2809212/1‑147 (MQ=255)
                                     ggACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCgg                                                                                                              <  1:4982960/151‑1 (MQ=255)
                                     ggACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAACCTGGTGTAGGACCgg                                                                                                              >  2:2976060/1‑151 (MQ=255)
                                      gACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCggg                                                                                                             <  1:356556/151‑1 (MQ=255)
                                       actcgcAGCATCTGGAGGGCCGGGTACTGCGGTCCATGGCCTGATGCTTTGCAACCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACTGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGt                                                                                                            <  1:1056206/148‑1 (MQ=25)
                                       aCGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGt                                                                                                            <  2:1672429/151‑1 (MQ=255)
                                        cgcgCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCgggct                                                                                                           >  2:3509198/1‑149 (MQ=255)
                                         gcgcAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGttt                                                                                                          >  2:1132316/1‑151 (MQ=255)
                                          ggcagcaTCTGGGGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTa                                                                                                         <  2:2450794/150‑1 (MQ=255)
                                          cgcAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTa                                                                                                         <  1:2061203/151‑1 (MQ=255)
                                           gcagcaTCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAACCTGGTGTAGGAGCGGGTTTAc                                                                                                        >  1:838748/1‑151 (MQ=255)
                                             agcaTCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACGACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTAccc                                                                                                      >  2:5058219/1‑151 (MQ=255)
                                             agcaTCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTAccc                                                                                                      >  1:1345932/1‑151 (MQ=255)
                                                  cTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGaa                                                                                                 <  2:1369378/151‑1 (MQ=255)
                                                   gggAGGGCGGGGTACTGCGGTCGATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAAt                                                                                                <  2:890040/150‑1 (MQ=255)
                                                     gAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCT‑GCGTAGGAGCGGGTTTACCCGCGAATACg                                                                                             >  2:393025/1‑151 (MQ=255)
                                                       gggCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAACCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACgg                                                                                            <  2:3920125/151‑1 (MQ=255)
                                                        ggCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGt                                                                                           <  2:595820/151‑1 (MQ=255)
                                                          cGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAg                                                                                         >  2:2357484/1‑151 (MQ=255)
                                                          cGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAg                                                                                         <  1:4118692/151‑1 (MQ=255)
                                                           ggggTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAgc                                                                                        <  2:4478486/151‑1 (MQ=255)
                                                              gTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAgcagc                                                                                     >  1:5116253/1‑148 (MQ=255)
                                                              gTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGACCGGGTTACCCCGCGAATACGGTagcagc                                                                                     >  2:399830/1‑151 (MQ=255)
                                                                 cTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTCCCCGCGAATACGGTAGCagcggc                                                                                  >  1:2220294/1‑151 (MQ=255)
                                                                     ggTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACg                                                                              <  2:1732462/151‑1 (MQ=255)
                                                                         cATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGa                                                                          >  2:2147918/1‑151 (MQ=255)
                                                                          aTGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGaa                                                                         <  1:3507556/151‑1 (MQ=255)
                                                                           tGGCTTGATGCTTTGCAGCCTGGCCTGGCCTCTTCGCGGGTAACCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAAc                                                                        <  1:1722791/151‑1 (MQ=17)
                                                                           tGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTACACCACCACTCTGGTGTAGGAGCGGGTTTACCCGCGAATCCGGTAGCAGCGGCAACGGTGAAc                                                                        >  1:4202240/1‑151 (MQ=255)
                                                                              ccTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGACTACGGTAGCGCCGGCCACGGGCAAcggg                                                                     >  2:2886217/1‑150 (MQ=18)
                                                                              ccTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAAcggc                                                                     <  1:1005815/151‑1 (MQ=255)
                                                                                 gATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCggg                                                                  <  2:1411575/151‑1 (MQ=255)
                                                                                 gATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCggg                                                                  >  1:284036/1‑151 (MQ=255)
                                                                                 gATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCggg                                                                  <  1:4460174/151‑1 (MQ=255)
                                                                                  gtGCTTGGCGGCCTGTACGGGCGTCTGCGCGGGGAAACCCGCGCCTACACGACGGCCGGGGCCCAACCACCGATGGGTGTCCACCACCAATCTGGTGGAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGt                                                                 <  2:3413546/150‑1 (MQ=14)
                                                                                     cTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCCCCACCAATCCGGTGTAGGAGCGGGATTACCCGCGAAGACGGTAGCAGCGGCAACGGTGAACTGCGGGTGGa                                                              >  1:3689908/1‑151 (MQ=17)
                                                                                     cTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGa                                                              <  2:3745150/151‑1 (MQ=255)
                                                                                         gCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAAtt                                                          <  2:5137445/151‑1 (MQ=255)
                                                                                          cAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTg                                                         >  2:589726/1‑151 (MQ=255)
                                                                                              cTGTACTGGCCTCTTCGCGGGTAACCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCca                                                     <  1:2470019/151‑1 (MQ=255)
                                                                                              cTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTCGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGCGTGGAAATTGGCca                                                     >  1:3355008/1‑151 (MQ=255)
                                                                                              cTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCca                                                     <  1:3696824/151‑1 (MQ=255)
                                                                                              cTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCca                                                     >  2:4910981/1‑151 (MQ=255)
                                                                                               agggccggCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAATCACCGATGTGGGTCCACCACCAATCCGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGTAATTGGCca                                                     <  2:2711067/144‑1 (MQ=11)
                                                                                                gTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCcagca                                                  <  1:393025/151‑1 (MQ=255)
                                                                                                  gccggCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAATCACCGACCTGTGTCCACCACCAATCCGGCGTAGGAGCGGGTTTACACGCGAAGAGCGTAGCGGCGGCAACGGGGGAAGGCCGGGGGGAATGGGCcagcag                                                 >  2:1120599/4‑151 (MQ=11)
                                                                                                  aCTGGCCTGTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCcagcag                                                 <  2:4782520/151‑1 (MQ=255)
                                                                                                        ctctTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCCGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCgg                                           >  2:2513205/1‑151 (MQ=255)
                                                                                                         tctTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCgg                                           >  2:3095094/1‑150 (MQ=255)
                                                                                                          ctTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAATCACCGACCTGTGTCCACCACCAATcc                                                                                                                           <  2:3554087/69‑2 (MQ=11)
                                                                                                             cgcgGGTAAGCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCctt                                      <  2:4329184/151‑1 (MQ=11)
                                                                                                             cgcgGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCACCAGCCCCGGccccg                                      >  2:4504654/1‑148 (MQ=14)
                                                                                                              tcgGGTAATCGCTCTCTTACTCGACGGCTTGCTCCCAACCACCGATGTGTGTCCTCCACCAATCTGGTGTAGAAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCcttt                                     <  1:2648066/150‑1 (MQ=11)
                                                                                                              gcgGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGGGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCcag                                                    >  1:1235174/1‑136 (MQ=255)
                                                                                                              gcgGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGGGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCcag                                                    <  2:1235174/136‑1 (MQ=255)
                                                                                                              gcgGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCcttt                                     >  1:4653019/1‑151 (MQ=255)
                                                                                                                 ggTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTcgc                                  <  1:2421394/151‑1 (MQ=255)
                                                                                                                  gTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTcgcg                                 >  1:2703434/1‑151 (MQ=11)
                                                                                                                  gTAAACCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTcgcg                                 <  1:2921427/151‑1 (MQ=255)
                                                                                                                      aCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTa                             <  2:3937301/151‑1 (MQ=11)
                                                                                                                      aCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTa                             <  1:1062295/151‑1 (MQ=11)
                                                                                                                      aCCCGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTa                             >  1:1796713/1‑151 (MQ=255)
                                                                                                                       cccGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTaa                            <  1:48132/151‑2 (MQ=11)
                                                                                                                        ccGCTCCTACACGCCGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTaaa                           <  1:449170/151‑3 (MQ=11)
                                                                                                                        ccGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTaaa                           >  1:5030118/1‑149 (MQ=11)
                                                                                                                         cGCTCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTaaac                          <  2:3187375/151‑4 (MQ=11)
                                                                                                                          gCTCCTACACGGGGGCCTGCGCCCAACCACCGATGAGTGGCCACCACCACTCTGGTGTAGGAGCGGGTTTAGCCGCGAAGACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTaaacg                         <  2:695154/151‑5 (MQ=11)
                                                                                                                            tCCTACACGACGGCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCATCAGGGCCGGCCCTTTCGCGGGTaaacggc                       >  1:4541887/1‑145 (MQ=14)
                                                                                                                                aaacggcgGCCTGTGTCCAACCACCGATGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGa                  >  1:2714991/7‑151 (MQ=11)
                                                                                                                                 aacggcgGCCTGTGTCCAACCACCGATGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGAt                 >  2:3144883/2‑151 (MQ=11)
                                                                                                                                   cggcgGCCTGTGTCCAACCACCGATGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCa               <  1:3116143/148‑1 (MQ=11)
                                                                                                                                      cgGCCTGTGTCCAACCACCGGTGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCACTc            <  1:2326895/151‑1 (MQ=14)
                                                                                                                                       ggCCTGCGCCCAACCACCGATGTGTGTCCACCACCAATCTGGGGTAGGAGCGGGTTTACCCGCGAATACGGTAGCAGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCCTTTCGCGGGTACAGGCAGGCGATCACTc            >  1:4398269/1‑151 (MQ=17)
                                                                                                                                          ctgtgtccAAGCACCGATGTGGGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCACTCagag        <  2:2000893/145‑1 (MQ=14)
                                                                                                                                          ctgtgtccAACCACCGATGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCACTCagag        >  1:119871/7‑151 (MQ=14)
                                                                                                                                              ctccAACCACCCATGTGTGTCCACCACCAATCT‑GTGTAGGAGCGGGTTTACCCGCGAATACGGTAGTGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCACTCAGAGCggg    <  2:895030/149‑1 (MQ=17)
                                                                                                                                                    |                                                                                                                                                   
GTTTGATGAGCAGCCGCAGAATGTTCACAGTTAGAGAGGACGCGCAGCATCTGGAGGGCGGGGTACTGCGGTCCATGGCCTGATGCTTTGCAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGACGGCCTGCGCCCAATCACCGATGTGTGTCCACCACCAATCTGGTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGTGGAAATTGGCCAGCAGGCCCGGCCTTTTCGCGGGTACAGGCAGGCGATCACTCAGAGCGGGCG  >  CP009974/3161561‑3161856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑