Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,230,201 C→T A23V (GCC→GTC)  RPPX_14410 → cell division protein FtsQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,230,2010CT97.9% 844.8 / ‑5.5 236A23V (GCC→GTC) RPPX_14410cell division protein FtsQ
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (140/89);  minor base G (2/3);  total (142/94)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GGCCCGCGCGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGTACGCCGA  >  CP009974/3230053‑3230348
                                                                                                                                                    |                                                                                                                                                   
ggcCCGCGCGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCa                                                                                                                                                   <  1:4008032/151‑1 (MQ=255)
ggcCCGCGCGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCa                                                                                                                                                   >  2:3081371/1‑151 (MQ=255)
ggcCCGCGCGGCTGGCCTGGACTTCCAGCAAATGGTGCTGGCAATCCTGGCCGAAAGGGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGAAAGCCGGTGCCGCGTGGTGTCa                                                                                                                                                   <  1:2052802/151‑1 (MQ=255)
 gcCCGCGCGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAg                                                                                                                                                  >  1:1333140/1‑151 (MQ=255)
   ccGCGCGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGcc                                                                                                                                                >  1:1515215/1‑151 (MQ=255)
    ggcgcgGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCg                                                                                                                                               <  2:2106101/150‑1 (MQ=255)
         ggCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCCAGCCGGTGCCGCGTGGTGTCAGCCGGCtgg                                                                                                                                          >  2:3174446/1‑151 (MQ=255)
         ggCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCtgg                                                                                                                                          >  2:3711174/1‑151 (MQ=255)
           cTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCtggtg                                                                                                                                        <  2:1464109/151‑1 (MQ=255)
            tGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCtggtgg                                                                                                                                       <  1:2801250/151‑1 (MQ=255)
            tGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCtggtgg                                                                                                                                       >  2:3888883/1‑151 (MQ=255)
             ggCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGc                                                                                                                                      >  1:2171673/1‑151 (MQ=255)
                cTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCcga                                                                                                                                   >  2:1079532/1‑151 (MQ=255)
                cTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCcga                                                                                                                                   >  1:4809707/1‑151 (MQ=255)
                   gACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCcgacga                                                                                                                                <  2:4228331/151‑1 (MQ=255)
                     cTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGc                                                                                                                              >  1:5083258/1‑151 (MQ=255)
                      ttCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGcc                                                                                                                             >  1:4402505/1‑151 (MQ=255)
                       tCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCGACCCGTTACCGGCCATAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGccc                                                                                                                            >  2:5017144/1‑151 (MQ=255)
                             aaCTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCg                                                                                                                      >  1:1061299/1‑151 (MQ=255)
                                  tggCTGGCAATCCTGGCCGAAAGCGAGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGgcgcg                                                                                                                 <  1:1652348/149‑1 (MQ=255)
                                  gTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGgcgcg                                                                                                                 >  1:1879981/1‑151 (MQ=255)
                                               tGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCa                                                                                                    >  2:859582/1‑151 (MQ=255)
                                                 gCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAg                                                                                                  <  2:2769211/151‑1 (MQ=255)
                                                   cGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGcc                                                                                                >  2:217980/1‑151 (MQ=255)
                                                   cGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGcc                                                                                                >  1:3641637/1‑151 (MQ=255)
                                                    gAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGccc                                                                                               >  2:3942154/1‑150 (MQ=255)
                                                    gAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCt                                                                                               >  1:1917140/1‑151 (MQ=255)
                                                    gAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCt                                                                                               >  1:730975/1‑151 (MQ=255)
                                                     aaaGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTg                                                                                              >  2:1423101/1‑151 (MQ=255)
                                                          gTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGggcgg                                                                                         >  1:4156055/1‑151 (MQ=255)
                                                            ggCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAACCCTGGGCGGcc                                                                                       >  2:5213772/1‑151 (MQ=255)
                                                             gCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCt                                                                                      <  2:2080960/151‑1 (MQ=255)
                                                              cGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTc                                                                                     >  2:298774/1‑151 (MQ=255)
                                                               gACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCa                                                                                    >  1:3655987/1‑151 (MQ=255)
                                                                 cgcgAGGTTAATGACCATGGCAGGGGGGATGATACGTCGGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAg                                                                                  <  2:3291853/151‑1 (MQ=255)
                                                                 cgcgAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAg                                                                                  >  1:3379391/1‑151 (MQ=255)
                                                                  gcgAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAgc                                                                                 >  1:3183638/1‑151 (MQ=255)
                                                                  gcgAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGCCCAAGCCTGGGCGGCCTCAAgc                                                                                 >  1:2614558/1‑151 (MQ=255)
                                                                  gcgAGGTTAATAACCAGGCACGGCGCGATGATACGTCAGCAGCACCCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAgc                                                                                 <  1:5022288/151‑1 (MQ=255)
                                                                     aGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTAGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCt                                                                              >  2:3098400/1‑151 (MQ=255)
                                                                      ggTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCtt                                                                             <  2:2478749/151‑1 (MQ=255)
                                                                       gTTAATAGCCATGCAAGAGGGGATGAGACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGGGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTg                                                                            <  2:1730247/151‑1 (MQ=255)
                                                                       gTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTg                                                                            >  2:2764151/1‑151 (MQ=255)
                                                                       gTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTg                                                                            >  1:2904654/1‑151 (MQ=255)
                                                                        ttAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCAAAGCCTGGGCGGCCTCAAGCGCTTGc                                                                           >  1:4598038/1‑151 (MQ=255)
                                                                            taaCCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCtgtg                                                                       <  1:2219613/151‑1 (MQ=255)
                                                                            taaCCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCtgtg                                                                       >  1:369207/1‑151 (MQ=255)
                                                                              aCCATGCAAGGCGCGATGATACGGCAGCAGCAACCCGTGACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGc                                                                     <  2:2287223/151‑1 (MQ=255)
                                                                              aCCAGGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGc                                                                     <  2:2622656/151‑1 (MQ=255)
                                                                                  tGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGgt                                                                 <  1:3206658/151‑1 (MQ=255)
                                                                                  tGCAAGGCGCGATGATACGTCAGAAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCggg                                                                 >  2:205592/1‑150 (MQ=255)
                                                                                    cAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCCAGCCTGGGCGGCCGCACGCGCCTGCTCTGGCCGctgt                                                               >  2:5152150/1‑151 (MQ=255)
                                                                                    cAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGgtgt                                                               >  2:2906568/1‑151 (MQ=255)
                                                                                    cAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGgtgt                                                               <  1:5044567/151‑1 (MQ=255)
                                                                                       ggCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGc                                                            >  1:3161835/1‑151 (MQ=255)
                                                                                       ggCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGc                                                            >  1:4772775/1‑151 (MQ=255)
                                                                                        gcgcGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCt                                                           <  1:442221/151‑1 (MQ=255)
                                                                                         cgcgATGATACGTCAGCAGCAACCCGTTGCCGGCCGTCGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCtg                                                          <  1:1708561/151‑1 (MQ=255)
                                                                                          gcgATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGCGTTGCtgg                                                         >  2:2560456/1‑151 (MQ=255)
                                                                                           cgATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCtggt                                                        >  2:1124617/1‑151 (MQ=255)
                                                                                            gatgatACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCtggtg                                                       >  2:2327311/1‑151 (MQ=255)
                                                                                            gatgatACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCtggtg                                                       >  2:3808538/1‑151 (MQ=255)
                                                                                            gatgatACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCtggtg                                                       >  2:2425298/1‑151 (MQ=255)
                                                                                            gatgatACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGCCCGGTGTTGCtgggg                                                       >  2:875522/1‑149 (MQ=255)
                                                                                             atgatACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCtggtgg                                                      >  1:3950310/1‑151 (MQ=255)
                                                                                                atACGGCAGCAGCAACCCGTTTCCGGCCGCAGCGAGCCGGTGCCTCGTCGTGTCAGCCGGCTGGTGGGCGACGAGCCCGTCTCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTggcgg                                                   <  2:4006519/151‑1 (MQ=255)
                                                                                                 tACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTggcggc                                                  >  2:3751535/1‑151 (MQ=255)
                                                                                                  aggTCAGCGGCAACCCGTTCCCGGCCGTAGCACGCCGGGGCCGCGTGGTGTCAGCCGGCCGGGGGCTGACGGGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGcggcc                                                 <  2:4984930/149‑1 (MQ=255)
                                                                                                  aCGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGcggcc                                                 >  1:3868872/1‑151 (MQ=255)
                                                                                                     tCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGcggccggc                                              >  1:2316104/1‑151 (MQ=255)
                                                                                                       agcagcAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCtt                                            >  1:560778/1‑151 (MQ=255)
                                                                                                          agcaACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTGTgg                                         >  2:4478734/1‑151 (MQ=255)
                                                                                                             aaCCCGTTACGGGCCGAAGCAAGCCGGTGCCGCGTGGTGGCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGc                                      <  1:2532893/151‑1 (MQ=255)
                                                                                                             aaCCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGcggcc                                                 >  2:1978959/1‑140 (MQ=255)
                                                                                                             aaCCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGcggcc                                                 <  1:1978959/140‑1 (MQ=255)
                                                                                                              aCCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGcc                                     >  2:128998/1‑151 (MQ=255)
                                                                                                              aCCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGcc                                     <  2:69173/151‑1 (MQ=255)
                                                                                                               cccGTTACCGGCCGTAGCACGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGAGCTTGCTGTGGCCGGTGTTGCTGGTGGCAGCCGGCCTTgtcgctc                                    >  2:3031422/1‑145 (MQ=255)
                                                                                                                 cGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTAc                                  <  1:5123344/151‑1 (MQ=255)
                                                                                                                 cGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTAc                                  >  2:4872176/1‑151 (MQ=255)
                                                                                                                  gTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACg                                 >  1:4545514/1‑151 (MQ=255)
                                                                                                                  gTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACg                                 >  1:4651670/1‑151 (MQ=255)
                                                                                                                    tACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAg                               >  1:350107/1‑151 (MQ=255)
                                                                                                                          ccGTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGcc                         <  1:2698885/151‑1 (MQ=255)
                                                                                                                            gTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCACGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGATGCTGGTGGCGGCCGGCTATGGTGCCTACGAGGGCGCCAt                       >  2:4426069/1‑151 (MQ=255)
                                                                                                                            gTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCAt                       >  1:3536550/1‑151 (MQ=255)
                                                                                                                            gTAGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCAt                       <  1:1784217/151‑1 (MQ=255)
                                                                                                                             tAGCAAGCCGGTGCCGCGGGGTGTCTGCCGGCTGGTGGCCGACGGGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCAtt                      <  2:171703/151‑1 (MQ=255)
                                                                                                                              aGCAAGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTc                     <  2:3358949/151‑1 (MQ=255)
                                                                                                                                 aaGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGcc                  >  2:2393537/1‑151 (MQ=255)
                                                                                                                                  aGCCGGTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGCCGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCCGCTTTGGTGCCTACGAGGGCGGCATTCGCCt                 >  2:4099678/1‑151 (MQ=255)
                                                                                                                                      ggTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATg             <  2:1879981/151‑1 (MQ=255)
                                                                                                                                      ggTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATg             >  1:4094513/1‑151 (MQ=255)
                                                                                                                                       gTGCCGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGc            >  1:1533900/1‑151 (MQ=255)
                                                                                                                                          ccGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGt         >  1:1144246/1‑151 (MQ=255)
                                                                                                                                          ccGCGTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGt         <  2:3554701/151‑1 (MQ=255)
                                                                                                                                          ccGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGt         <  1:974383/151‑1 (MQ=255)
                                                                                                                                             cgTGGTGTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGTACg      <  1:2145862/151‑1 (MQ=255)
                                                                                                                                               tggtgTCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGTACGcc    <  1:1021197/151‑1 (MQ=255)
                                                                                                                                               gggtgGCAGGCGGGTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGGTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGTACGcc    <  2:2705279/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCCGCGCGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCGTATCGGCGCGCCTGCCCCGGCCAAGCCTGGGCGGCCTCAAGCGCTTGCTGTGGCCGGTGTTGCTGGTGGCGGCCGGCTTTGGTGCCTACGAGGGCGCCATTCGCCTGATGCCGTACGCCGA  >  CP009974/3230053‑3230348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑