Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,400,067 A→G Q266Q (CAA→CAG)  RPPX_15160 → chemotaxis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,400,0670AG99.6% 972.6 / ‑5.7 274Q266Q (CAA→CAG) RPPX_15160chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (131/142);  total (131/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 274 total aligned reads displayed.

GCAGCGTATCGTGCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAACGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGACCTGGTG  >  CP009974/3399920‑3400214
                                                                                                                                                   |                                                                                                                                                   
     gTATCGTGCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTtcgtc                                                                                                                                             >  2:329290/1‑151 (MQ=255)
     gTATCGTGCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTtcgtc                                                                                                                                             <  1:5047935/151‑1 (MQ=255)
       ctCGTGCATGCCCTCGACAAGCTGCGTCCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCCACGAAATGCCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCCGCGTCCGTCgg                                                                                                                                           >  2:3382734/2‑151 (MQ=255)
          gTGCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGc                                                                                                                                        <  2:2502184/151‑1 (MQ=255)
            gCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAg                                                                                                                                      <  1:2818059/151‑1 (MQ=255)
            gCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAg                                                                                                                                      <  1:4677087/151‑1 (MQ=255)
                      gACGAGCTGCGGTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAACTGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACtt                                                                                                                            <  2:2148192/151‑1 (MQ=255)
                      gACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACtt                                                                                                                            <  2:705176/151‑1 (MQ=255)
                       acgaGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTc                                                                                                                           <  2:632906/148‑1 (MQ=255)
                        cAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCg                                                                                                                          >  1:1238429/1‑151 (MQ=255)
                        cAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGACACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCg                                                                                                                          <  2:5059911/151‑1 (MQ=255)
                           gCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTc                                                                                                                       <  1:319987/151‑1 (MQ=255)
                           gCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTc                                                                                                                       >  2:5164790/1‑151 (MQ=255)
                             tGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCAc                                                                                                                     <  2:4179705/151‑1 (MQ=255)
                              gCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCAcc                                                                                                                    <  1:3080488/151‑1 (MQ=255)
                               cGTTCCGGTGACCTGAGCATGCGGCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGCAAGCGGCGTCCACGAAATGGCCGAGGCACGGGCCAACCTCGTGGCCCAGGCCCAGCGGTCGTCGGTGCAGGTGGCCACTGCGGTCACCg                                                                                                                   >  2:4698985/1‑151 (MQ=255)
                               cGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCg                                                                                                                   <  1:908828/151‑1 (MQ=255)
                                gTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGa                                                                                                                  >  1:4938610/1‑151 (MQ=255)
                                 ttCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGaa                                                                                                                 <  2:4394302/151‑1 (MQ=255)
                                  tCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGaaa                                                                                                                >  1:1001805/1‑151 (MQ=255)
                                    cGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAAtt                                                                                                              >  1:3845542/1‑151 (MQ=255)
                                     ggTGGCCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGCCACCGACAtgg                                                                                                             >  2:4305307/1‑149 (MQ=255)
                                        gACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTgccg                                                                                                          >  1:5157327/1‑151 (MQ=255)
                                          ccTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTgccgcc                                                                                                        >  1:4291894/1‑151 (MQ=255)
                                           cTGAGCGTGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCa                                                                                                       <  2:3516409/151‑1 (MQ=255)
                                            tGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCAc                                                                                                      <  1:2524074/151‑1 (MQ=255)
                                            tGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCAc                                                                                                      <  1:2092722/151‑1 (MQ=255)
                                               gCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTc                                                                                                   >  1:3977766/1‑151 (MQ=255)
                                                 aTGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGCCGGTGCAGGAGACCACTCCGGCCACCGCCATTGCCGCCACCTCCa                                                                                                 >  2:612946/1‑151 (MQ=255)
                                                    cgcCGGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGc                                                                                              >  2:4318867/1‑151 (MQ=255)
                                                      ccTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCaa                                                                                            <  1:158176/151‑1 (MQ=255)
                                                        tGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAAca                                                                                          <  1:242110/151‑1 (MQ=255)
                                                        tGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAAca                                                                                          >  1:3361593/1‑151 (MQ=255)
                                                         gAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCCCCAAGCAAcag                                                                                         >  2:4046281/1‑151 (MQ=255)
                                                           gCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCACGCAAcagca                                                                                       >  2:2850308/1‑151 (MQ=255)
                                                             cTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAgg                                                                                     >  1:5184244/1‑151 (MQ=255)
                                                               ggATCGCAAGGACGACTTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGATCGTCGGTGCAGGCGACCACTTAGGCCACCGAAATTCCCGCCACCTCCCAGCAACAGCAGgcc                                                                                   >  2:4444361/1‑151 (MQ=255)
                                                                 aTCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccac                                                                                 <  1:3091670/151‑1 (MQ=255)
                                                                  tCGCAAGGATGAATTCGGTGCCATCGAAAGCCGCTTCAACCGGATGGCCGAGGCACTTGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccacc                                                                                <  2:265532/151‑1 (MQ=255)
                                                                  tCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccacc                                                                                >  1:3726321/1‑151 (MQ=255)
                                                                  tCGCAAGGACGAATGCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccacc                                                                                <  1:184322/151‑1 (MQ=255)
                                                                    gCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccaccgc                                                                              >  1:1993678/1‑151 (MQ=255)
                                                                     cAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccaccgcc                                                                             <  1:1439758/151‑1 (MQ=255)
                                                                     cAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccaccgcc                                                                             >  2:239530/1‑151 (MQ=255)
                                                                      aaGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTTATCACTTCGGTCACCGAAATTGCCGACACCTACAAGAAACAGCAGgcaaccgccc                                                                            >  1:405106/1‑150 (MQ=255)
                                                                      aaGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccaccgcca                                                                            <  1:222394/151‑1 (MQ=255)
                                                                      aaGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccaccgcca                                                                            >  1:3519656/1‑151 (MQ=255)
                                                                        ggACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGgccaccgccacc                                                                          <  1:1346943/151‑1 (MQ=255)
                                                                         gACGATTTCGGTGCCATCGAAGGCGGCTTCACCGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCTGGCAACAGCAGGccacggccaccg                                                                         <  2:3188945/151‑1 (MQ=255)
                                                                          aCGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCga                                                                        <  1:758659/151‑1 (MQ=255)
                                                                          aCGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCga                                                                        >  2:779752/1‑151 (MQ=255)
                                                                           cGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCgag                                                                       >  1:3871372/1‑151 (MQ=255)
                                                                           cGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCgag                                                                       <  1:520358/151‑1 (MQ=255)
                                                                            gAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCgaga                                                                      >  1:2936682/1‑151 (MQ=255)
                                                                            gAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCgaga                                                                      <  1:4836351/151‑1 (MQ=255)
                                                                            gAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCCCCAAGCAACAGCAGGCCACCGCCACCgaga                                                                      >  1:4547866/1‑151 (MQ=255)
                                                                             agttCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCGCCGCCACCGAGAc                                                                     <  2:3067685/149‑1 (MQ=255)
                                                                               ttCGGTGCCATCGAAAGCGGCTTCAACGCAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCCCCGAGACgg                                                                   >  2:2611469/1‑151 (MQ=255)
                                                                                tCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGgc                                                                  >  2:1983345/1‑151 (MQ=255)
                                                                                 cGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGgcc                                                                 >  1:567001/1‑151 (MQ=255)
                                                                                       cATCGAAAGCGGCTTAAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGccacc                                                           <  2:4620853/151‑1 (MQ=255)
                                                                                        aTCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGccaccc                                                          >  2:1504953/1‑150 (MQ=255)
                                                                                        aTCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGccacca                                                          >  2:1201256/1‑151 (MQ=255)
                                                                                        aTCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGCCACCGAAATTGCCGCCACCTCCAAGCAACCGCAGGCCACCGCCACCGCGACGGCCGccacca                                                          >  2:1741890/1‑151 (MQ=255)
                                                                                         tCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCCCCACCGAGACGGCCGccaccac                                                         >  2:3863389/1‑151 (MQ=255)
                                                                                            aaaGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCcaccaccac                                                      >  2:2978543/1‑151 (MQ=255)
                                                                                               gccgCTTCAACGACATGGCCGAGGCACTGGCCAACCTGGTGTCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGa                                                   <  2:5178498/148‑1 (MQ=255)
                                                                                               gCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGa                                                   <  1:4207873/151‑1 (MQ=255)
                                                                                                    ttCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCg                                              >  1:2360356/1‑151 (MQ=255)
                                                                                                    ttCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAACCg                                              >  2:4843657/1‑151 (MQ=255)
                                                                                                          gAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGccc                                        >  2:2762735/1‑150 (MQ=255)
                                                                                                          gAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCa                                        >  2:2566869/1‑151 (MQ=255)
                                                                                                          gAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCa                                        >  2:2233067/1‑151 (MQ=255)
                                                                                                           aaaTGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCAc                                       >  1:3248640/1‑151 (MQ=255)
                                                                                                               ggCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTcg                                   >  2:2330608/1‑151 (MQ=255)
                                                                                                                 ccGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTcgcg                                 >  2:3932092/1‑151 (MQ=255)
                                                                                                                  cGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGACATCGGCGCCACCTCGCgg                                >  1:4374138/1‑151 (MQ=255)
                                                                                                                  cGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCgg                                >  2:744057/1‑151 (MQ=255)
                                                                                                                   gAGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCggg                               >  1:3008867/1‑151 (MQ=255)
                                                                                                                    aGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGa                              >  2:1789052/1‑151 (MQ=255)
                                                                                                                    aGGCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGa                              >  1:304614/1‑151 (MQ=255)
                                                                                                                      gCACTGGCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGaaa                            <  1:332651/151‑1 (MQ=255)
                                                                                                                        aCTGGCCAACCTGGTGGCCCAGGCGCAGCGTGCGTCGGTGCAGGTGACCACGTCGGTCACCGAAATTGCCGCCACCTCCATGCATCATCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATc                          <  2:4752883/151‑1 (MQ=255)
                                                                                                                           ggCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATcgcc                       <  2:1676226/151‑1 (MQ=255)
                                                                                                                           ggCCAACCTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCCCGCGGGAAATcgcc                       >  2:855940/1‑151 (MQ=255)
                                                                                                                                  cTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACtt                <  1:444426/151‑1 (MQ=255)
                                                                                                                                  cTGGTGGCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACtt                <  1:3643746/151‑1 (MQ=255)
                                                                                                                                    ggtggCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGCAAGCGGCGCCACCGCGCGGGAGACCGCcgcgccggcg              >  2:4784759/1‑145 (MQ=255)
                                                                                                                                      tggCCCAGGCCCAGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTcgcg            >  1:4635471/1‑151 (MQ=255)
                                                                                                                                       ggcccaggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCgg           <  2:459159/151‑1 (MQ=255)
                                                                                                                                         cccaggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGa         <  2:3248640/151‑1 (MQ=255)
                                                                                                                                         cccaggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGa         <  1:5018150/151‑1 (MQ=255)
                                                                                                                                         cccaggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCAAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGa         <  1:686157/151‑1 (MQ=255)
                                                                                                                                          ccaggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGAc        <  1:364907/151‑1 (MQ=255)
                                                                                                                                           caggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGAcc       >  1:1801041/1‑151 (MQ=255)
                                                                                                                                            aggcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGGCGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGACCt      <  1:1648072/151‑1 (MQ=255)
                                                                                                                                              gcccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGACCTgg    <  2:2679226/151‑1 (MQ=255)
                                                                                                                                                ccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGACCTGGTg  >  1:1074533/1‑151 (MQ=255)
                                                                                                                                                ccaGCGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCGCGCGGGAAATCGCCGCGACTTCGCGGGACCggggg  >  2:494940/1‑149 (MQ=255)
                                                                                                                                                ccaGCGTTCGTCGGTGCAGGTGACCACTGCGGTCACCGAGCTTGCCGCCACCTCCAAGCAACAGCAGGCCGCCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGACCTGGTg  <  2:3208700/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAGCGTATCGTGCATGCCCTCGACAAGCTGCGTTCCGGTGACCTGAGCATGCGCCTGAGCCTGGATCGCAAGGACGAATTCGGTGCCATCGAAAGCGGCTTCAACGAAATGGCCGAGGCACTGGCCAACCTGGTGGCCCAGGCCCAACGTTCGTCGGTGCAGGTGACCACTTCGGTCACCGAAATTGCCGCCACCTCCAAGCAACAGCAGGCCACCGCCACCGAGACGGCCGCCACCACCACCGAAATCGGCGCCACCTCGCGGGAAATCGCCGCGACTTCGCGGGACCTGGTG  >  CP009974/3399920‑3400214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑