Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,400,410 A→G T381A (ACC→GCC)  RPPX_15160 → chemotaxis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,400,4100AG99.6% 876.5 / NA 245T381A (ACC→GCC) RPPX_15160chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (117/127);  total (117/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

GGCTGGCTCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTACCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATCGAGCAAATG  >  CP009974/3400262‑3400556
                                                                                                                                                    |                                                                                                                                                  
tgctggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGcc                                                                                                                                                  >  1:3316977/2‑151 (MQ=255)
ggctggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGcc                                                                                                                                                  <  1:1009495/151‑1 (MQ=255)
 gctggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCg                                                                                                                                                 <  1:3709991/151‑1 (MQ=255)
  ctggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCga                                                                                                                                                <  2:4481633/151‑1 (MQ=255)
  ctggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCga                                                                                                                                                <  2:1468085/151‑1 (MQ=255)
   tggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCACGGTTGCCgac                                                                                                                                               >  2:1504426/1‑151 (MQ=255)
   tggctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCgac                                                                                                                                               <  1:2330608/151‑1 (MQ=255)
     gctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCgacca                                                                                                                                             >  2:4747510/1‑151 (MQ=255)
      ctCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCgaccag                                                                                                                                            <  1:744057/151‑1 (MQ=255)
         cGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCgaccagacc                                                                                                                                         >  2:3371842/1‑151 (MQ=255)
             cagcagGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATc                                                                                                                                     <  2:2181940/151‑1 (MQ=255)
               gcagGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATctg                                                                                                                                   <  1:3490557/151‑1 (MQ=255)
                 agGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCAAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGACGGTGGTAACCATCGTCAAGGCCGCCGACAAGACCACTctgcc                                                                                                                                 >  2:4804831/1‑150 (MQ=255)
                    gcatagCACGCATGGAAGAGACCATGGACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTc                                                                                                                              <  2:405106/146‑1 (MQ=255)
                    gCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTc                                                                                                                              >  2:661717/1‑151 (MQ=255)
                     ccTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTTCTGCCg                                                                                                                             >  2:2619945/1‑151 (MQ=255)
                        ggCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTc                                                                                                                          <  1:309597/151‑1 (MQ=255)
                         gCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCa                                                                                                                         >  1:2938754/1‑151 (MQ=255)
                         gCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCa                                                                                                                         >  1:88908/1‑151 (MQ=255)
                           aCGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAAc                                                                                                                       <  1:5112683/151‑1 (MQ=255)
                           aCGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAAc                                                                                                                       <  2:1665319/151‑1 (MQ=255)
                             gCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAAcgc                                                                                                                     <  1:4748172/151‑1 (MQ=255)
                             gCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAAcgc                                                                                                                     <  1:837693/151‑1 (MQ=255)
                               aTGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAAcgccg                                                                                                                   >  1:2480143/1‑151 (MQ=255)
                                 ggAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACgccgcc                                                                                                                 <  2:4261037/151‑1 (MQ=255)
                                    agagACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATc                                                                                                              <  2:3292616/151‑1 (MQ=255)
                                    agagACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATc                                                                                                              <  1:3058156/151‑1 (MQ=255)
                                       gaCCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGaa                                                                                                           <  2:3906137/151‑1 (MQ=255)
                                        aCCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAg                                                                                                          <  2:3847129/151‑1 (MQ=255)
                                          cATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCg                                                                                                        >  1:526362/1‑151 (MQ=255)
                                            tGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATAGTCAAGGTTGCCGACCAGAGCAATCTGCTGTAGATAAACGCAGACATAGAAGCGGa                                                                                                      >  2:3737784/1‑151 (MQ=255)
                                              cACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGaaa                                                                                                    >  2:2993252/1‑151 (MQ=255)
                                               aCCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGACGCGGaaaa                                                                                                   >  2:2525204/1‑151 (MQ=255)
                                                  aGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGc                                                                                                >  2:1932885/1‑151 (MQ=255)
                                                  aGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGc                                                                                                >  2:3488837/1‑151 (MQ=255)
                                                    gTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCg                                                                                              <  2:5129899/151‑1 (MQ=255)
                                                     tGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCgg                                                                                             <  1:577032/151‑1 (MQ=255)
                                                       aTGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCggag                                                                                           >  1:2686111/1‑149 (MQ=255)
                                                            cgcTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAatat                                                                                      >  1:1126279/1‑151 (MQ=255)
                                                            cgcTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAatat                                                                                      <  1:1446446/151‑1 (MQ=255)
                                                             gcTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATg                                                                                     >  1:2737248/1‑151 (MQ=255)
                                                                 ccGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAACAGGCCGCTGAATATGGCcg                                                                                 >  2:4765183/1‑151 (MQ=255)
                                                                  cGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCcgc                                                                                <  1:795004/151‑1 (MQ=255)
                                                                    aCCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCgg                                                                              >  2:2857817/1‑151 (MQ=255)
                                                                    aCCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGACATCGAAGCGGAAACGGCCGGTGAATATGGCCGCgg                                                                              >  2:3258485/1‑151 (MQ=255)
                                                                      cTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCt                                                                            >  2:633186/1‑151 (MQ=255)
                                                                       tGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCtt                                                                           <  1:516632/151‑1 (MQ=255)
                                                                        ggTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCttt                                                                          <  2:3843630/151‑1 (MQ=255)
                                                                        ggTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCttt                                                                          >  1:4911203/1‑151 (MQ=255)
                                                                         gTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTg                                                                         <  1:3187425/151‑1 (MQ=255)
                                                                         gTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTg                                                                         >  2:1315222/1‑151 (MQ=255)
                                                                         gTCAATGCCAAGCTCGGTATCCGCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTg                                                                         <  2:440783/151‑1 (MQ=255)
                                                                          tCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGc                                                                        <  1:4091176/151‑1 (MQ=255)
                                                                           cAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTCTGcc                                                                       >  2:2595589/1‑151 (MQ=255)
                                                                             aTGCAAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGt                                                                     <  1:4619720/151‑1 (MQ=255)
                                                                               gCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTgg                                                                   >  2:4884562/1‑151 (MQ=255)
                                                                                    gCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTcgcg                                                              <  2:3531113/151‑1 (MQ=255)
                                                                                    gCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTcgcg                                                              <  1:4240289/151‑1 (MQ=255)
                                                                                       cGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGAcc                                                           <  1:2601661/151‑1 (MQ=255)
                                                                                          tATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGaa                                                        >  1:4542613/1‑151 (MQ=255)
                                                                                          tATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGaa                                                        >  2:102490/1‑151 (MQ=255)
                                                                                           aTCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAg                                                       >  1:1537487/1‑151 (MQ=255)
                                                                                            tCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGt                                                      >  2:1826281/1‑151 (MQ=255)
                                                                                             ccTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTg                                                     <  2:3574974/151‑1 (MQ=255)
                                                                                                cAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTgcgc                                                  >  2:1768632/1‑151 (MQ=255)
                                                                                                  aCGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGcgccg                                                <  2:3343143/151‑1 (MQ=255)
                                                                                                  aCGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGcgccg                                                <  1:1789052/151‑1 (MQ=255)
                                                                                                    gagaAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCt                                              <  2:1239501/151‑1 (MQ=255)
                                                                                                       aaaGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTgg                                           >  1:3026159/1‑151 (MQ=255)
                                                                                                       aaaGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTgg                                           >  1:1738565/1‑151 (MQ=255)
                                                                                                           ccAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGGCgc                                       >  2:5218890/1‑150 (MQ=255)
                                                                                                           ccAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGa                                       <  2:340274/151‑1 (MQ=255)
                                                                                                            cAGCAACAGCACCCAGAGGGTGGTGACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGAc                                      <  2:2825446/151‑1 (MQ=255)
                                                                                                                aaCATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAgc                                  >  2:816712/1‑150 (MQ=255)
                                                                                                                aaCATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGa                                  >  1:3354740/1‑151 (MQ=255)
                                                                                                                 aCATCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGAc                                 >  1:2809491/1‑151 (MQ=255)
                                                                                                                   aTCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGGCCCGAccg                               >  1:3040001/1‑151 (MQ=255)
                                                                                                                   aTCACCCAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGAccg                               >  2:1103246/1‑151 (MQ=255)
                                                                                                                        ccAGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTg                          >  2:321340/1‑151 (MQ=255)
                                                                                                                          aGATGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGCCCAGACCCCCGCGGc                        >  2:2763610/1‑151 (MQ=255)
                                                                                                                            aTGGTGGTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCa                      <  1:3911428/151‑1 (MQ=255)
                                                                                                                            aTGGTGGTAACCATCGCCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCa                      <  2:2370924/151‑1 (MQ=255)
                                                                                                                             tggtggTAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCAc                     >  1:4598202/1‑151 (MQ=255)
                                                                                                                               gtggtAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCt                   >  2:832340/1‑151 (MQ=255)
                                                                                                                               gtggtAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCt                   >  1:4228469/1‑151 (MQ=255)
                                                                                                                                tggtAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTa                  >  1:974753/1‑151 (MQ=255)
                                                                                                                                 ggtAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTAc                 >  1:2127053/1‑151 (MQ=255)
                                                                                                                                  gtAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTAcc                >  2:2040147/1‑150 (MQ=255)
                                                                                                                                  gtAACCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACg                >  1:3241442/1‑151 (MQ=255)
                                                                                                                                    aaCCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGAc              >  2:4347111/1‑151 (MQ=255)
                                                                                                                                     aCCATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACa             <  1:3804125/151‑1 (MQ=255)
                                                                                                                                       cATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATc           >  2:1050836/1‑151 (MQ=255)
                                                                                                                                       cATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATc           >  2:5173388/1‑151 (MQ=255)
                                                                                                                                       cATCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATc           >  1:2535279/1‑151 (MQ=255)
                                                                                                                                         tCGTCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATCGa         <  1:4421777/151‑1 (MQ=255)
                                                                                                                                           gTCAAGGTTGCCGACCAGACCAATCTGCTGCCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCACGGCTTTGCCGTGGTCGCTACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGACACCGACGACATCGAGc       >  2:2297335/1‑151 (MQ=255)
                                                                                                                                            tCAAGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATCGAGCa      >  1:3431497/1‑151 (MQ=255)
                                                                                                                                             cAAGGTTGCCGACTAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGTTCAGACCGCCGTGGCCACCTACGACATCGAGCaa     >  2:1271358/1‑151 (MQ=255)
                                                                                                                                              aaGGTTGCCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATCGAGCaaa    >  1:486735/1‑151 (MQ=255)
                                                                                                                                                ggTTGCCGACCAGACCAATCTGCTGTCGCGCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATCGAGCAAATg  <  1:1883472/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCTGGCTCCGGGCAGCAGGGCCTGGCACGCATGGAAGAGACCATGCACCAGGTGATGGGCGCTGCCGACCTGGTCAATGCCAAGCTCGGTATCCTCAACGAGAAAGCCAGCAACATCACCCAGATGGTGGTAACCATCGTCAAGGTTACCGACCAGACCAATCTGCTGTCGCTCAACGCCGCCATCGAAGCGGAAAAGGCCGGTGAATATGGCCGCGGCTTTGCCGTGGTCGCGACCGAAGTGCGCCGCTTGGCCGACCAGACCGCCGTGGCCACCTACGACATCGAGCAAATG  >  CP009974/3400262‑3400556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑