Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,673,509 C→T intergenic (+108/‑429) RPPX_16400 → / → RPPX_16405 glucose‑1‑phosphate thymidylyltransferase/beta‑ketoacyl synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,673,5090CT83.4% 857.3 / 129.2 384intergenic (+108/‑429)RPPX_16400/RPPX_16405glucose‑1‑phosphate thymidylyltransferase/beta‑ketoacyl synthase
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base T (150/166);  total (181/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

CCAGTATCTGCTGCGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTCCCATCAGCCGTTCACCGTCGCCGCCG  >  CP009974/3673362‑3673658
                                                                                                                                                   |                                                                                                                                                     
     aTCTGCTGCGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTAtt                                                                                                                                               >  2:2902501/1‑151 (MQ=255)
       ctgctgCGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTcg                                                                                                                                             <  1:1211465/151‑1 (MQ=255)
        tgctgcGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTcgc                                                                                                                                            <  2:2608801/151‑1 (MQ=255)
          ctgcGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCgg                                                                                                                                          <  2:3064405/151‑1 (MQ=255)
          ctgcGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGCGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGCCGGGACCGAGCCCTGCTGGCCAACTTCCACCCGCCGTCCCCCGTTGCCGGCGCTACCGCATTcgcgc                                                                                                                                          >  2:3390041/1‑150 (MQ=255)
           tgcGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCggg                                                                                                                                         >  1:1782378/1‑151 (MQ=255)
            gcGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCCCGGGt                                                                                                                                        >  2:1884037/1‑151 (MQ=255)
             cGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTa                                                                                                                                       >  2:1321378/1‑151 (MQ=255)
              gTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTaa                                                                                                                                      >  2:4315051/1‑151 (MQ=255)
                 tGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAAcc                                                                                                                                   >  2:4061679/1‑151 (MQ=255)
                  gATTCAAGGCACGGTTTATTGAGGCAACACCCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAAccc                                                                                                                                  <  1:2830371/151‑1 (MQ=255)
                   aTTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCg                                                                                                                                 >  1:3971638/1‑151 (MQ=255)
                               gTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGa                                                                                                                     <  1:3103385/151‑1 (MQ=255)
                               gTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGAGGGTAAACCCGCTCCTCCACAGa                                                                                                                     >  2:5148856/1‑151 (MQ=255)
                                tttATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGAt                                                                                                                    >  2:1071909/1‑151 (MQ=255)
                                 ttATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACCCAGAtt                                                                                                                   >  2:4631003/1‑151 (MQ=255)
                                 ttATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGAtt                                                                                                                   >  2:1638438/1‑151 (MQ=255)
                                  tATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATtg                                                                                                                  <  2:1508094/151‑1 (MQ=255)
                                   aTTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATtgg                                                                                                                 <  1:2472768/151‑1 (MQ=255)
                                      gAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATtggtgg                                                                                                              <  1:2305308/151‑1 (MQ=255)
                                          caacaaCCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGa                                                                                                          >  1:1166154/1‑151 (MQ=255)
                                           aacaacCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGac                                                                                                         >  2:2942645/1‑151 (MQ=255)
                                                     gTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCggg                                                                                               >  1:2556420/1‑151 (MQ=255)
                                                        gggCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGt                                                                                            >  1:4495464/1‑151 (MQ=255)
                                                        gggCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGt                                                                                            >  2:1162544/1‑151 (MQ=255)
                                                           cggcgCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTgg                                                                                         >  1:3303141/1‑151 (MQ=255)
                                                            ggcgCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCgggg                                                                                              >  1:2746009/1‑145 (MQ=255)
                                                            ggcgCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGa                                                                                        <  1:4114301/151‑1 (MQ=255)
                                                             gcgcCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATGCGTGGTCGTCACACCTCGCGGGTTCGac                                                                                       >  2:4461308/1‑151 (MQ=18)
                                                                       tCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTGCCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCcgtc                                                                             >  2:4881189/1‑151 (MQ=255)
                                                                       tCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCcgtc                                                                             <  2:3648568/151‑1 (MQ=255)
                                                                       tCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCcgtc                                                                             <  1:5233950/151‑1 (MQ=255)
                                                                         cACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGTCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCcgtcgt                                                                           >  1:29816/1‑151 (MQ=255)
                                                                          accggCGGTTCCACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGAGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCgtg                                                                          <  1:2178706/148‑1 (MQ=255)
                                                                             ggCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAg                                                                       <  1:2941667/151‑1 (MQ=255)
                                                                             ggCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAg                                                                       >  2:4123978/1‑151 (MQ=255)
                                                                             ggCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAg                                                                       <  1:4552802/151‑1 (MQ=255)
                                                                              gCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAgg                                                                      <  1:2902501/151‑1 (MQ=255)
                                                                                ggTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAg                                                                    <  1:5195692/151‑1 (MQ=255)
                                                                                   tCAACGCGCGAAGGGACCGAGTCCTGCTGGCCAATGTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCGCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCgg                                                                 <  2:2647440/151‑1 (MQ=14)
                                                                                   tCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCAACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCAGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCgg                                                                 <  2:395895/151‑1 (MQ=255)
                                                                                    cAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCggg                                                                >  1:4971515/1‑151 (MQ=255)
                                                                                      aCCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGtt                                                              <  2:2082305/151‑1 (MQ=255)
                                                                                       cccGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGttt                                                             <  1:3802553/151‑1 (MQ=255)
                                                                                        ccGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTa                                                            >  1:1450945/1‑151 (MQ=255)
                                                                                          gcgAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTAcc                                                          >  2:1674832/1‑151 (MQ=255)
                                                                                           ggAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTAccc                                                         <  2:2798003/150‑1 (MQ=255)
                                                                                                   gccggcccTGCTGGCCAATTTCCACCCGCCGTTCACTGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCTGGGGTTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAAgg                                                 <  1:2063652/145‑1 (MQ=11)
                                                                                                   ccGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAgg                                                 <  1:1342863/151‑1 (MQ=255)
                                                                                                      ggcccTGCTGGCCAATTTCCACCCGCCGTTCACTGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCTGGGGTTGGACACAGGCCGTCGTGTAGGAg                                                                    >  2:731687/4‑129 (MQ=1)
                                                                                                      aGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAgggcc                                              >  1:5135994/1‑151 (MQ=255)
                                                                                                        tCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGCGGTGCACACCCCTCGGGGGTTGGACACAGGCCGCCGTGTAGGAGCGGGTTCACCCGCGAAAcggcccg                                            >  2:3712408/1‑149 (MQ=11)
                                                                                                         ccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAgggccggc                                           >  1:558932/1‑150 (MQ=11)
                                                                                                          ctgctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAgggccggcc                                          >  1:4172323/1‑149 (MQ=11)
                                                                                                           tgctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAgggccggccc                                         <  1:4802423/151‑1 (MQ=11)
                                                                                                            gctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCct                                        >  1:953980/1‑151 (MQ=255)
                                                                                                            gctgGCCAATGTCCACCCGCCGTTCACTGTTGCCGGCGCTAGCGTATGCGCGGGGAACCCCGGTCCTACACAGATTGGTGGTGGACACACCTCTGGGGTTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCct                                        <  2:4231777/151‑1 (MQ=14)
                                                                                                            ccgggCCAATTTCCACCCGCCGTTCACTGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCTGGGGTTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCct                                        <  1:4639700/148‑1 (MQ=14)
                                                                                                              tgGCCAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAACGGGCCGGGCctgc                                      >  1:4865812/1‑151 (MQ=255)
                                                                                                              tgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCctgc                                      <  2:3337207/151‑1 (MQ=11)
                                                                                                              tgGCCAATTTCCACCCGCCCTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCctgctg                                    >  1:367181/1‑151 (MQ=11)
                                                                                                               ggCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCctgct                                     <  2:5075135/151‑1 (MQ=11)
                                                                                                                 ccAATTTCCACCCGCCGTTCACTGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCTGGGGTTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTgg                                   <  1:1371964/151‑1 (MQ=14)
                                                                                                                 ccAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTgg                                   <  1:1961393/151‑1 (MQ=11)
                                                                                                                  cagtcgccACCCGCGCGTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGAGTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGc                                  <  2:3786070/145‑1 (MQ=11)
                                                                                                                  cAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGc                                  <  1:3745661/151‑1 (MQ=255)
                                                                                                                  cAATTTCCACCCGCCGTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCGCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGc                                  <  1:260981/151‑1 (MQ=255)
                                                                                                                   catctcctccCGCCCTTCACTGTTGCTGCCGCCATCGTATTCGCGGGTAAACCCGCTCATACACAGATTGGTGGTGGACACACATCGGTGATTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCag                               <  2:1962569/147‑2 (MQ=1)
                                                                                                                   aaTTTCCACCCGCCCTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCaa                               >  1:3535989/1‑151 (MQ=11)
                                                                                                                    agctCCACCCGCCGTGCACCGTTGCCGCCGCCAGCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCa                                <  1:2733199/148‑1 (MQ=11)
                                                                                                                    actaccaccaCCCCTTGACCGATGCCGCCGCCACCGTATTCGAGGGTAAACCCGCACCTACAAAGAATGGTAATGGACACAAATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAAt                              <  2:2373759/147‑1 (MQ=2)
                                                                                                                      ttCCACCGGCCGTGCACTGTTGCCGCCGCTACGGTATGCGCGGGTAACCCCGCTCCTACACAGATTGGTGGTGGACACACCTCTGGGGTTGGACACGGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAAt                              <  2:3528715/151‑1 (MQ=14)
                                                                                                                      ttCCACCCGCCCTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAAttt                            <  1:1285697/151‑2 (MQ=11)
                                                                                                                      ttCCACCCGCCCTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAAttt                            >  1:2124692/1‑150 (MQ=11)
                                                                                                                        ccACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAAtct                            <  1:1084466/151‑3 (MQ=11)
                                                                                                                         cACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAAtctc                           <  1:3580307/151‑4 (MQ=11)
                                                                                                                         cACCCGCCACGCACTGTTGCTGCCGCCGTCGTATTCGCGGGTAAGCCCGCTCCTTCACAGATTGGTGGTGGACACACATCGGTGGTTGGACACAGCCCG‑C‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAGTTTCCa                         <  2:5223135/151‑1 (MQ=11)
                                                                                                                          aCCCGCCCTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAGTTTCCac                        >  2:677819/1‑150 (MQ=1)
                                                                                                                            ccgccgTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTCCCAt                        >  1:1824627/1‑151 (MQ=255)
                                                                                                                             cgccgTGCCCCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAAtctccacc                       <  2:64582/151‑8 (MQ=11)
                                                                                                                                cgTTCACCGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTCCCATCAGc                    >  1:1668685/1‑151 (MQ=255)
                                                                                                                                 cttCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAATTTCCACCAGccat                 <  2:254240/150‑3 (MQ=11)
                                                                                                                                  ttCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGCGCGGGCTCACCCGCGAAAGGGCCGGCCCTGCTGGCCCATCTCCACccgccg                  >  2:832760/1‑151 (MQ=2)
                                                                                                                                  ttCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAATTTCCACCAGCCAtt                <  1:4596886/151‑1 (MQ=11)
                                                                                                                                   tCACCGTTGCCGCCGCTACCGTATTCGCGAGTAAACCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGt                 >  1:2672879/1‑151 (MQ=2)
                                                                                                                                   tCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCACCAGCCGt                 <  1:3448992/151‑1 (MQ=11)
                                                                                                                                   ccACCGGCGCCGCCGCCACCGTATGCGCGGGAAATCCCGGGCCTACACAGATTGGTGACGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAATTTCCACCAGCCATTc               <  2:3467377/150‑1 (MQ=2)
                                                                                                                                     cccGTTGCCGGCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTCCCATCAGCCGTTc               <  1:4045572/150‑1 (MQ=255)
                                                                                                                                     cccGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCACCAGCCGTTc               <  1:720224/150‑1 (MQ=11)
                                                                                                                                      ccGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCACCAGCCGTTCa              >  1:3239323/1‑151 (MQ=14)
                                                                                                                                      ccGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGATGGACACACATCGGTGGTTGGACACA‑GCCCAC‑TGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAATTTCCACCAGCCATGCAcc            >  1:3859402/1‑151 (MQ=11)
                                                                                                                                        gTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTGGGAGCGGGCTTACGCGCGAAGAGGCCGGCCCTGCTGCCCAATCTCCACCAGCCGCTCAcc            >  2:4529703/1‑151 (MQ=11)
                                                                                                                                         ttggccccgccAGCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCACCAGCCGTTCACCg           <  1:924875/145‑1 (MQ=1)
                                                                                                                                         ttGCCGCCGCTACCGTATTTGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGGACACACCTCTGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCGTGCTGGCCAATTTTCACCCGCCGTTCAcct           >  2:3126887/1‑150 (MQ=14)
                                                                                                                                          tGCCGCCGCGAGGGTAGTCGGGGGTAAACCCGCTGCTACACAGATTGGTGGTGGACACGCCTCTGGGGTTGGACACAGGCCGACGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCCCTGCTGGCCAATTTCCACCCGCCGTTCACTGt          <  2:2220588/151‑1 (MQ=11)
                                                                                                                                           gccgccgcgACCTTATGCGCGGGTAACCCCGCTCCTGCGCAGATGGGTTGTGGACACACACCGGAGGTTGGACGCAGGCCGTCGTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGTTCACCGtt         <  1:4677336/151‑2 (MQ=2)
                                                                                                                                            ccgccgccACCGTATTCGCGGGTAAGCCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCACCAGCCGTTCACCGttg        <  1:4365881/151‑3 (MQ=11)
                                                                                                                                             cgccgccACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCCATCTCCACCAGCCGTTCACCGTtgc       >  1:1616013/1‑151 (MQ=11)
                                                                                                                                              ggcgctACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTCCCATCAGCCGTTCACCGTcgcc      <  1:4313022/149‑1 (MQ=255)
                                                                                                                                               ccgccACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACATCGGGGGCTGGACACAGGCCGCCGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCACCAGCCGTTCACCGTtgccg     >  1:151413/1‑151 (MQ=11)
                                                                                                                                                   |                                                                                                                                                     
CCAGTATCTGCTGCGTTTGATTCAAGGCACGGTTTATTGAGGCAACAACCTCGGTGGGGCGGCGCCGCCATTCCACGGGCGGTTCAACCCGCGAAGGGACCGAGTCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACAGATTGGTGGTGGACACACCTCGGGGGTTGGACACAGGCCGTCGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTCCCATCAGCCGTTCACCGTCGCCGCCG  >  CP009974/3673362‑3673658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑