Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,837,790 T→C intergenic (+341/‑210) RPPX_17095 → / → RPPX_17100 proteophosphoglycan precursor/cupin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,837,7900TC97.5% 356.4 / ‑4.4 120intergenic (+341/‑210)RPPX_17095/RPPX_17100proteophosphoglycan precursor/cupin
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (67/48);  total (68/52)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 121 total aligned reads displayed.

AATTTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGTCGTTCACCATTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCGGAT  >  CP009974/3837646‑3837937
                                                                                                                                                |                                                                                                                                                   
aaTTTTCACCAGCCGTTCACCGTTGCTGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACTGCTCTGGACTGCGCGGTCCTCTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCGGCCGGCCAATCTTCACCAGCCGCTCAccg                                                                                                                                            >  2:2348121/1‑150 (MQ=2)
                                                          cTACACACGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTCTTCCCGGGTAAACCCGCTCCAGCACACGCATCggtggt                                                                                     >  1:1326978/1‑151 (MQ=11)
                                                             cacacGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGtggttgg                                                                                  >  1:5047004/1‑151 (MQ=9)
                                                              acacGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGtggttggt                                                                                 >  2:4655247/1‑151 (MQ=255)
                                                              acacGCACCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGtggttggt                                                                                 <  1:1541744/151‑1 (MQ=255)
                                                               cacGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCTCACGCAGCGGGGGTTGGTc                                                                                >  1:2670419/1‑151 (MQ=11)
                                                                acGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCCCACGCATCGGTGGTTGGTca                                                                               >  2:4849748/1‑151 (MQ=255)
                                                                  gcggccggggCGGCCGAGGCCGTCGTCGGCGCGGGTCCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTcaca                                                                             <  2:5218817/144‑1 (MQ=11)
                                                                  gCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTcaca                                                                             >  2:612151/1‑151 (MQ=255)
                                                                   cagcgtgggCGGCACAGGCCGTTGTAGGCGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAg                                                                            <  1:1603120/145‑1 (MQ=11)
                                                                   cATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAg                                                                            >  2:1404092/1‑151 (MQ=255)
                                                                    aTCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCCTCGGGGGTTGGTCACAgg                                                                           >  1:4154472/1‑151 (MQ=11)
                                                                       gAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCcg                                                                        <  1:2746024/151‑1 (MQ=255)
                                                                       gAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCcg                                                                        >  1:2729628/1‑151 (MQ=255)
                                                                        aGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCcgt                                                                       >  2:4352168/1‑151 (MQ=255)
                                                                         gggCGGCGCAGGCCGTGGTAGGCGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTAGTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCcgtc                                                                      <  2:4955671/151‑1 (MQ=14)
                                                                            cggcACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTa                                                                   <  1:4766392/151‑1 (MQ=255)
                                                                            cggcACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTa                                                                   >  1:1106779/1‑151 (MQ=255)
                                                                             ggcACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCCCCCGCTCACGCCCCGGTGGCTGGGCACAGGCCGTCGCAg                                                                  >  2:195752/1‑151 (MQ=11)
                                                                              gcACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAgg                                                                 >  2:842508/1‑151 (MQ=255)
                                                                               cacaGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGa                                                                >  1:3362563/1‑151 (MQ=255)
                                                                               cacaGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGa                                                                <  2:1106779/151‑1 (MQ=255)
                                                                                acaGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAg                                                               <  1:2329531/151‑1 (MQ=255)
                                                                                acaGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGCAg                                                               >  2:1561242/1‑151 (MQ=255)
                                                                                  aGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCg                                                             >  1:2060636/1‑151 (MQ=255)
                                                                                   ggCCGTTGTAGGCGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCCCCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCgg                                                            <  2:1981728/151‑1 (MQ=14)
                                                                                   ggCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCgg                                                            <  2:3095580/151‑1 (MQ=255)
                                                                                   ggCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCgg                                                            >  2:415579/1‑151 (MQ=255)
                                                                                      cGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGtt                                                         >  2:1662721/1‑151 (MQ=255)
                                                                                       gTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTc                                                        >  1:82339/1‑151 (MQ=255)
                                                                                          ttAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCAcc                                                     <  1:4482402/150‑1 (MQ=255)
                                                                                           tAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCAccc                                                    <  1:3276144/151‑1 (MQ=255)
                                                                                            aGGAGCGGGTACACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcg                                                   <  2:332421/151‑1 (MQ=255)
                                                                                             ggAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                                  >  1:1933904/1‑151 (MQ=255)
                                                                                             ggAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                                  >  1:1734243/1‑151 (MQ=255)
                                                                                             ggAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                                  >  1:1933816/1‑151 (MQ=255)
                                                                                             ggAGCGGGTGCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                                  <  1:4484701/151‑1 (MQ=255)
                                                                                               aGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGa                                                >  2:4539898/1‑151 (MQ=255)
                                                                                               aGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGa                                                <  2:3737219/151‑1 (MQ=255)
                                                                                                gCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGCGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCCaa                                               >  2:2661177/1‑151 (MQ=14)
                                                                                                 cGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGaaa                                              >  1:2879766/1‑151 (MQ=255)
                                                                                                  gggTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGaaaa                                             >  1:1727971/1‑151 (MQ=255)
                                                                                                   ggtaaaCCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAg                                            <  2:1462053/146‑1 (MQ=14)
                                                                                                   ggTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAg                                            >  1:3132835/1‑151 (MQ=255)
                                                                                                   ggTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAg                                            <  1:1309753/151‑1 (MQ=255)
                                                                                                   ggTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAg                                            >  1:1159365/1‑151 (MQ=255)
                                                                                                    gtggagccGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGACGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAgg                                           <  2:2263540/145‑1 (MQ=14)
                                                                                                         cccGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGt                                      >  1:3900569/1‑151 (MQ=255)
                                                                                                           cgcgAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGCGCGGGTTCACCCGCGAACAGGCCGGTAc                                    >  2:4709308/1‑151 (MQ=14)
                                                                                                           cgcgAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTAc                                    <  1:1175574/151‑1 (MQ=255)
                                                                                                            gcgAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACa                                   >  1:782524/1‑151 (MQ=255)
                                                                                                              gAAAGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAgg                                 <  1:3086456/151‑1 (MQ=255)
                                                                                                              gAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGTCGTTCACCATTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                                  <  2:515672/134‑1 (MQ=255)
                                                                                                              gAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGTCGTTCACCATTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCcgc                                                  >  1:515672/1‑134 (MQ=255)
                                                                                                               caaGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGc                                <  1:3507664/150‑1 (MQ=255)
                                                                                                               aaaGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGc                                <  1:5003643/151‑1 (MQ=255)
                                                                                                               aaaGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGc                                <  1:4418896/151‑1 (MQ=255)
                                                                                                                aaGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCg                               >  2:659539/1‑151 (MQ=255)
                                                                                                                aaGGGCCGGGCCTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCg                               <  1:4216980/151‑1 (MQ=255)
                                                                                                                 aGGGCCGGGCCTGCTGGCCAATTGCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGGGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGa                              <  2:2306565/151‑1 (MQ=255)
                                                                                                                   ggccgggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGCAAAGGCCGGTACAGGCGaaa                            >  2:4558870/1‑151 (MQ=255)
                                                                                                                   ggccgggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGCTTCACCCGCGAAAAGGCCGGTACAGGCGaaa                            >  2:4058156/1‑151 (MQ=255)
                                                                                                                     ccgggccTGCTGTCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGTCAACCCCGCCCCTGCACACGCATCGCTCGTCGGTCACAGGCCGTCGTAGGACCTGGTTCACCCGCGAAACGGCCCGTCCCAGCGAAAAt                          >  2:3755987/1‑151 (MQ=14)
                                                                                                                     ccgggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGACGAAAAt                          >  2:3218224/1‑151 (MQ=255)
                                                                                                                      cgggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGCAAATc                         >  2:3334103/1‑151 (MQ=255)
                                                                                                                      cgggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATc                         >  1:2082343/1‑151 (MQ=255)
                                                                                                                       gggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAACTCg                        >  2:3241373/1‑151 (MQ=255)
                                                                                                                       gggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCg                        <  1:2226269/151‑1 (MQ=255)
                                                                                                                       gggccTGCGGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCg                        <  2:302803/151‑1 (MQ=255)
                                                                                                                       gcgccTGCTGGCCAATTTCCACCCTCGGTTCACCGTCGCCGCCGCTACCGTATTCGCGGGTGAACCCGCGCCTGCACACGCATCGGTGGTGGGTCACAGGCCGTGGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCg                        <  1:2226318/149‑1 (MQ=17)
                                                                                                                        ggccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCgg                       >  2:3653819/1‑151 (MQ=255)
                                                                                                                         gccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGc                      >  1:2711800/1‑151 (MQ=255)
                                                                                                                         gccTGCTGGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGCTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGc                      >  2:4013095/1‑151 (MQ=255)
                                                                                                                          ccTGCGGGCCAATTTCCCCCCGCGGCTCACCGTTGCGGCGGCGACCGTGTTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTGGGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGcc                     <  2:1396955/151‑1 (MQ=14)
                                                                                                                           ctgctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGccc                    >  1:4086133/1‑151 (MQ=255)
                                                                                                                           ctgctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGccc                    >  1:892767/1‑151 (MQ=255)
                                                                                                                            tgctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGcccc                   >  2:1349565/1‑151 (MQ=255)
                                                                                                                             gctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCCTCGGTGGTCGGTCACAGGCCGTCGTAGGAGCCGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAACCGGccccc                  >  2:4365754/1‑151 (MQ=17)
                                                                                                                             gctgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGccccc                  <  1:1869288/151‑1 (MQ=255)
                                                                                                                              ctgGCCACTTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCt                 <  2:1518846/151‑1 (MQ=255)
                                                                                                                               tgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTg                >  2:3407229/1‑151 (MQ=255)
                                                                                                                               tgGCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTg                <  1:821614/151‑1 (MQ=255)
                                                                                                                                ggCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGGTACCGTATTGGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGGGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGc               <  1:1949510/151‑1 (MQ=18)
                                                                                                                                ggCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGc               >  2:2110695/1‑151 (MQ=255)
                                                                                                                                ggCCAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGc               <  1:4872419/151‑1 (MQ=255)
                                                                                                                                  ccAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCtt             >  2:4689175/1‑151 (MQ=255)
                                                                                                                                   cAATTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCttt            <  2:4875736/151‑1 (MQ=255)
                                                                                                                                    aaTTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGTTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCtttt           <  1:2899447/151‑1 (MQ=255)
                                                                                                                                    aaTTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCtttt           <  2:724072/151‑1 (MQ=255)
                                                                                                                                    aaTTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCtttt           >  1:3090902/1‑151 (MQ=255)
                                                                                                                                    aaTTTCCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCCCTTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGACAATCGGCCCCCTGCtttt           >  1:1639820/1‑151 (MQ=18)
                                                                                                                                     atttcCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTc          <  2:2729628/148‑1 (MQ=255)
                                                                                                                                      tttcCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTcc         >  1:4143456/3‑151 (MQ=255)
                                                                                                                                      tttcCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTcc         <  1:2367061/149‑1 (MQ=255)
                                                                                                                                        tcCACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTcccc       >  2:3890398/1‑151 (MQ=255)
                                                                                                                                          cACCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCg     <  1:528771/151‑1 (MQ=255)
                                                                                                                                           ccccGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCgg    <  1:3588564/150‑1 (MQ=255)
                                                                                                                                           aCCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCgg    >  2:1331891/1‑151 (MQ=255)
                                                                                                                                           aCCCGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCgg    >  1:321053/1‑151 (MQ=255)
                                                                                                                                             ccgccgTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGGACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCGGAt  >  1:903283/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AATTTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGAGGGCGGCACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGTCGTTCACCATTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTGCACACGCATCGGTGGTTGGTCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAAGGCCGGTACAGGCGAAAATCGGCCCCCTGCTTTTCCCCCGGAT  >  CP009974/3837646‑3837937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: