Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,848,567 T→G D1030A (GAC→GCC)  RPPX_17160 ← histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,848,5670TG99.6% 801.9 / ‑4.8 229D1030A (GAC→GCC) RPPX_17160histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (106/122);  total (107/122)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 230 total aligned reads displayed.

CTGTGCAGATAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGTCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCTTGCTGA  >  CP009974/3848420‑3848714
                                                                                                                                                   |                                                                                                                                                   
 tgtgCAGATAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGCCAATTTCTGCGTCGTTGGGGGCGAc                                                                                                                                                 >  2:3645537/1‑151 (MQ=255)
 tgtgCAGATAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGGTGGGGGCGAc                                                                                                                                                 >  1:2495170/1‑151 (MQ=255)
       gATAACCCTGGTACAGCTGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGc                                                                                                                                           <  2:1727821/151‑1 (MQ=255)
        aTAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGCCAATGGCGCGGACACTTTCTGCGTCGTTGGGGGCGACCGGTGcc                                                                                                                                          >  2:433431/1‑150 (MQ=255)
        aTAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCa                                                                                                                                          <  1:537475/151‑1 (MQ=255)
        aTAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCa                                                                                                                                          <  2:1635712/151‑1 (MQ=255)
          aaCCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATc                                                                                                                                        >  1:771584/1‑151 (MQ=255)
              cTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGt                                                                                                                                    <  2:1907728/151‑1 (MQ=255)
               tGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTa                                                                                                                                   <  1:3263246/151‑1 (MQ=255)
                ggTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTAc                                                                                                                                  <  2:623829/151‑1 (MQ=255)
                ggTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTAc                                                                                                                                  >  1:4516487/1‑151 (MQ=255)
                        tGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCttt                                                                                                                          <  1:4939035/151‑1 (MQ=255)
                          gCACGCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGGTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGACGCGGACAATATCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGACGCTTTgg                                                                                                                        <  2:1279488/151‑1 (MQ=255)
                           cACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGt                                                                                                                       >  1:409324/1‑151 (MQ=255)
                           cACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCATTGGGAGCGACCGGTGCATCGCGTACGTAGCTTTGGt                                                                                                                       >  2:4093906/1‑151 (MQ=255)
                            aCCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGAcc                                                                                                                                                <  1:980221/125‑1 (MQ=255)
                            aCCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGCTGGGGGCGACCGGTGCATCGCGTACGATGCTATGGTc                                                                                                                      >  2:1092874/1‑151 (MQ=255)
                             ccccAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCa                                                                                                                     <  1:2128198/151‑1 (MQ=255)
                                 aGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAAttt                                                                                                                 >  1:4066757/1‑151 (MQ=255)
                                 aGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCACGCCGCCAACGGCGCGGACCATCTCTGCGTCGTTGGGGGCGACCGGGGAATCGCCTGCGAAGCTCTGGTCAAtat                                                                                                                 >  2:3875520/1‑149 (MQ=255)
                                  gCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAAtttt                                                                                                                <  1:1835828/151‑1 (MQ=255)
                                   ccGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTc                                                                                                               <  1:856217/151‑1 (MQ=255)
                                    cGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCa                                                                                                              <  1:5189610/151‑1 (MQ=255)
                                     gCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAg                                                                                                             <  1:5119366/151‑1 (MQ=255)
                                         gagaAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCa                                                                                                         <  1:3721744/151‑1 (MQ=255)
                                            aaaCGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCg                                                                                                      <  1:3559605/151‑1 (MQ=255)
                                             agcGCCCGCTGTTCGGTCAGCTCTCCCCCGTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGa                                                                                                     <  2:3867291/149‑1 (MQ=255)
                                             aaCGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCCTCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGa                                                                                                     >  1:2200290/1‑151 (MQ=255)
                                             aaCGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGa                                                                                                     >  2:2017187/1‑151 (MQ=255)
                                                gCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCg                                                                                                  <  1:1403835/151‑1 (MQ=255)
                                                gCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCg                                                                                                  <  2:4882103/151‑1 (MQ=255)
                                                gCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCg                                                                                                  <  1:4266294/151‑1 (MQ=255)
                                                 ccAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCgg                                                                                                 >  2:369195/1‑151 (MQ=255)
                                                 ccAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCgg                                                                                                 >  1:2252509/1‑151 (MQ=255)
                                                  cAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGc                                                                                                <  1:2835805/151‑1 (MQ=255)
                                                  cAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGc                                                                                                >  1:2647922/1‑151 (MQ=255)
                                                        ttCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACACTGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGgcgc                                                                                          >  1:1108893/1‑151 (MQ=255)
                                                           ggTCGGCTCGACCCCTTCGGCAATCACCGGCAGGTCCAGGCTGCGCGCCATGGCGACAATGGGGCGGACCATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTc                                                                                       <  2:4732566/151‑1 (MQ=255)
                                                           ggTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTc                                                                                       >  1:1378241/1‑151 (MQ=255)
                                                              cAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAgg                                                                                    <  2:2546796/151‑1 (MQ=255)
                                                              cAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCGTCAgg                                                                                    >  2:590184/1‑151 (MQ=255)
                                                               aGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGt                                                                                   <  2:3961669/151‑1 (MQ=255)
                                                               aGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGt                                                                                   <  1:1947513/151‑1 (MQ=255)
                                                                 ctctACCTCTTCGGCAATCGCCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTaa                                                                                 <  2:1442412/151‑1 (MQ=255)
                                                                 ctctACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTaa                                                                                 >  2:484269/1‑151 (MQ=255)
                                                                   ctACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGTCAATGGCGCGGACAATTTTTGTGTTGTTGGGGGCGACCGGTGCATCGCGTAAGAAGCGTTGGTCAATTTTAAGCGCATCGACCGGCAGGCGCTTCAGGTAAGt                                                                               >  2:1507166/1‑151 (MQ=255)
                                                                   ctACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGt                                                                               <  2:2053718/151‑1 (MQ=255)
                                                                    tACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTc                                                                              >  1:3508416/1‑151 (MQ=255)
                                                                      ccccTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAg                                                                            >  2:849531/1‑151 (MQ=255)
                                                                       cccTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGc                                                                           <  1:1604115/151‑1 (MQ=255)
                                                                          ttCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGCTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATAGACCGGCAGGCGCTTCAGGTAAGTCAGCGAg                                                                        >  2:2177657/1‑151 (MQ=255)
                                                                            cGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGa                                                                      <  2:3248725/151‑1 (MQ=255)
                                                                             ggCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACCATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGGACGAAGCTCTGGGCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCgaagaa                                                                     >  2:3398377/1‑151 (MQ=255)
                                                                               cAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATa                                                                   >  2:968819/1‑151 (MQ=255)
                                                                                aaTCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCg                                                                          >  1:1028180/1‑143 (MQ=255)
                                                                                aaTCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAg                                                                  >  1:2309436/1‑151 (MQ=255)
                                                                                  tCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGcc                                                                <  2:3796215/151‑1 (MQ=255)
                                                                                  tCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGcc                                                                <  2:242071/151‑1 (MQ=255)
                                                                                     ccGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGt                                                             >  1:4929244/1‑151 (MQ=255)
                                                                                     ccGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGt                                                             <  1:2626002/151‑1 (MQ=255)
                                                                                      cGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTg                                                            >  1:2075382/1‑151 (MQ=255)
                                                                                        ccAGGTCCAGGCTGCGCGCCATGGCGACGATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGcc                                                          <  2:1835036/151‑1 (MQ=255)
                                                                                        ccAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGcc                                                          <  1:481130/151‑1 (MQ=255)
                                                                                         cAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGCGGAATAGCCGGTGCCg                                                         >  2:3053863/1‑151 (MQ=255)
                                                                                         cAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCg                                                         >  2:3133471/1‑151 (MQ=255)
                                                                                         cAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCg                                                         >  1:3112886/1‑151 (MQ=255)
                                                                                              ccAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTc                                                    <  1:34578/151‑1 (MQ=255)
                                                                                                aGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCg                                                         <  1:1536110/144‑1 (MQ=255)
                                                                                                aGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCAt                                                  <  2:893908/151‑1 (MQ=255)
                                                                                                  gCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGAGCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATcc                                                >  2:3280721/1‑151 (MQ=255)
                                                                                                  gCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATcc                                                >  2:2973155/1‑151 (MQ=255)
                                                                                                  gCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATcc                                                <  2:2688276/151‑1 (MQ=255)
                                                                                                   cTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCa                                               >  1:4310317/1‑151 (MQ=255)
                                                                                                   cTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCa                                               <  2:1047764/151‑1 (MQ=255)
                                                                                                       gcgcCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGc                                           <  2:4263170/151‑1 (MQ=255)
                                                                                                        cgcCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCa                                          <  2:4030135/151‑1 (MQ=255)
                                                                                                          ccATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCaaa                                        <  1:749034/151‑1 (MQ=255)
                                                                                                           cATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCaaaa                                       >  2:1963415/1‑151 (MQ=255)
                                                                                                           cATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCaaaa                                       >  1:393699/1‑151 (MQ=255)
                                                                                                           cATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCACGGCaaaa                                       >  1:3972415/1‑151 (MQ=255)
                                                                                                            aTGGCGACAATGGCTCAGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAAc                                      <  2:3701760/151‑1 (MQ=255)
                                                                                                              ggCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTc                                    >  1:4854551/1‑151 (MQ=255)
                                                                                                              ggCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTc                                    <  1:2275162/151‑1 (MQ=255)
                                                                                                               gCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCa                                   <  2:3182660/151‑1 (MQ=255)
                                                                                                                cGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCAc                                  >  2:1311720/1‑151 (MQ=255)
                                                                                                                    aaTGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCa                              <  2:3850250/151‑1 (MQ=255)
                                                                                                                      tGGCGCGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATa                            <  1:4011420/151‑1 (MQ=255)
                                                                                                                        gcgcGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAgc                          >  1:3849726/1‑151 (MQ=255)
                                                                                                                        gcgcGGACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAgc                          <  2:1026996/151‑1 (MQ=255)
                                                                                                                            ggACAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCtt                      <  2:470674/151‑1 (MQ=255)
                                                                                                                              aCAATTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCa                    <  1:1311720/151‑1 (MQ=255)
                                                                                                                               cAATTTCTGCGTCGTTGGGGGCGCCCGGTGCATTGCTTACGAAGCTTTGGTCAATTTTCAGCGCTTCGACCGGCAGGCGCTTCAGGTAAGTCAACGAGGAATAGCCGGTGGCGATGTAATCCATAGACAAACTAAATCCATAACGCTTCAg                   >  2:4461729/1‑151 (MQ=255)
                                                                                                                                aaTTTCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGc                  >  1:2449569/1‑151 (MQ=255)
                                                                                                                                   ttCTGCGTCGTTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCGGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTcg               >  2:5022897/1‑151 (MQ=255)
                                                                                                                                         gtcgtTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATc         <  1:3172335/151‑1 (MQ=255)
                                                                                                                                          tcgtTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCt        >  1:1141727/1‑151 (MQ=255)
                                                                                                                                          tcgtTGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCt        <  1:1129835/151‑1 (MQ=255)
                                                                                                                                           cgtTGGGGGCGGCCGGTGCATCGCGTACGAAGCTTTGGTCAATGTTCCGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCtt       <  2:1237388/151‑1 (MQ=255)
                                                                                                                                              tGGGGGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCTTGCt    >  1:2667326/1‑151 (MQ=255)
                                                                                                                                               ggggGCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCTTGCTg   <  1:2108599/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGTGCAGATAACCCTGGTACAGATGGCACCCCAGCCGCTCGAGAAACGCCAGCTGTTCGGTCAGCTCTACCCCTTCGGCAATCACCGCCAGGTCCAGGCTGCGCGCCATGGCGACAATGGCGCGGACAATTTCTGCGTCGTTGGGGTCGACCGGTGCATCGCGTACGAAGCTTTGGTCAATTTTCAGCGCATCGACCGGCAGGCGCTTCAGGTAAGTCAGCGAGGAATAGCCGGTGCCGAAGTCATCCATGGCAAAACTCACCCCATAGCGCTTCAGCTCGCGCATCTTGCTGA  >  CP009974/3848420‑3848714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑