Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,971,951 G→T S9S (TCC→TCA)  RPPX_17675 ← hemolysin D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,971,9510GT98.5% 896.2 / NA 262S9S (TCC→TCA) RPPX_17675hemolysin D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (125/132);  minor base C (4/0);  total (130/132)
Fisher's exact test for biased strand distribution p-value = 5.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098
                                                                                                                                                      |                                                                                                                                                   
gTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCgggg                                                                                                                                                     >  1:412201/1‑151 (MQ=255)
 tAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCggggg                                                                                                                                                    >  2:3198351/1‑151 (MQ=255)
   ggCATCGTCGGTGGTGTCCAGGTAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTCCAGTTTCGGCGGGTGAg                                                                                                                                                  <  2:494405/151‑1 (MQ=255)
        cgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGttt                                                                                                                                             >  2:71600/1‑151 (MQ=255)
        cgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGttt                                                                                                                                             <  1:3990835/151‑1 (MQ=255)
          tcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGt                                                                                                                                           >  2:5079911/1‑151 (MQ=255)
            ggtggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCt                                                                                                                                         <  1:1478871/151‑1 (MQ=255)
              tggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       <  2:5138471/151‑1 (MQ=255)
              tggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTCTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       >  2:2063603/1‑151 (MQ=255)
                 tttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGc                                                                                                                                    <  1:2389184/151‑1 (MQ=255)
                      tggtAGAGGGCGTTGCTGAACCAGTAGCGGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAt                                                                                                                               <  2:4762526/148‑1 (MQ=255)
                      aGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAt                                                                                                                               >  1:1035564/1‑151 (MQ=255)
                      aGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAt                                                                                                                               >  1:3805416/1‑151 (MQ=255)
                       gATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAtt                                                                                                                              <  1:1661513/151‑1 (MQ=255)
                       gATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAtt                                                                                                                              <  1:5025541/151‑1 (MQ=255)
                        aTAGCGGGCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTc                                                                                                                             <  2:2339939/151‑1 (MQ=255)
                         tAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCa                                                                                                                            <  1:2582929/151‑1 (MQ=255)
                          aGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAt                                                                                                                           >  1:1730666/1‑151 (MQ=255)
                          aGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAt                                                                                                                           <  1:1169950/151‑1 (MQ=255)
                          aGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGAGGATTGCCAGGCGGCGCCGGGTTTTGGCGCGCGGCCGCTGTTCAGGTTCGGCGGGTGACGGTTTGTCTACGGCAACATTcct                                                                                                                           >  2:2938211/1‑149 (MQ=255)
                             ggCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggt                                                                                                                        <  1:2242827/151‑1 (MQ=255)
                              gCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACCTTCAtggtt                                                                                                                       >  1:2458742/1‑151 (MQ=255)
                                cGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggttgg                                                                                                                     <  1:5000287/151‑1 (MQ=255)
                                cGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggttgg                                                                                                                     >  2:3709749/1‑151 (MQ=255)
                                     cTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTcc                                                                                                                <  1:12162/151‑1 (MQ=255)
                                      tGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCa                                                                                                               <  2:3613340/151‑1 (MQ=255)
                                       gAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCAt                                                                                                              <  2:4201623/151‑1 (MQ=255)
                                       gAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCAt                                                                                                              >  2:583450/1‑151 (MQ=255)
                                         cccAGGAGCGGGTGAAGGCCGGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtc                                                                                                            <  2:741940/150‑1 (MQ=255)
                                           cAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtctc                                                                                                          <  2:4272768/151‑1 (MQ=255)
                                           cAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtctc                                                                                                          >  1:470122/1‑151 (MQ=255)
                                           cAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtctc                                                                                                          <  2:2169888/151‑1 (MQ=255)
                                            ggTAGCTGGTGGAGGCCAGCGGGGTGAGTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCg                                                                                                         <  2:4651463/150‑1 (MQ=255)
                                             gTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCCGGCGGCGACGGGTTTTAGCGCGCGGCCGCTGTTGCGTTTCGGCGGGCGCGGGTTTGTCTACGGCAACATGCATGGGTGGTTTCCATTCTCgc                                                                                                        >  2:4703453/1‑150 (MQ=255)
                                             gTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGa                                                                                                        <  1:1421321/151‑1 (MQ=255)
                                                 cTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGGATTg                                                                                                    >  1:858082/1‑151 (MQ=255)
                                                    gTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGc                                                                                                 <  2:2744415/151‑1 (MQ=255)
                                                    gTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGc                                                                                                 >  1:1103247/1‑151 (MQ=255)
                                                     tGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGCGAGGGTTTGTCTACGGCAACCTTCATGGTTGGTTTCCATTCTCGAATTGGGcc                                                                                                >  2:4832800/1‑151 (MQ=255)
                                                      gAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCct                                                                                               <  2:2461665/151‑1 (MQ=255)
                                                       aaGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctg                                                                                              >  2:3809041/1‑151 (MQ=255)
                                                        aGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgc                                                                                             <  1:2434556/151‑1 (MQ=255)
                                                        aGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgc                                                                                             >  2:3742072/1‑151 (MQ=255)
                                                         ggCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgct                                                                                            >  1:3448843/1‑151 (MQ=255)
                                                          gCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgctg                                                                                           >  1:2923293/1‑151 (MQ=255)
                                                            cagcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggc                                                                                         >  2:4342500/1‑151 (MQ=255)
                                                             agcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggct                                                                                        <  1:4356813/151‑1 (MQ=255)
                                                                agGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTc                                                                                     <  1:64017/151‑1 (MQ=255)
                                                                agGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTc                                                                                     <  2:893072/151‑1 (MQ=255)
                                                                agGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTc                                                                                     >  1:3146121/1‑151 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCg                                                                                    <  1:361112/151‑1 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGCTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCg                                                                                    >  1:4164123/1‑151 (MQ=255)
                                                                    tGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGtt                                                                                 <  1:592690/151‑1 (MQ=255)
                                                                      acggCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAGTTGGGCCTGCTGGCTGTCGGTtgt                                                                               <  2:2419806/148‑1 (MQ=255)
                                                                          cGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGa                                                                           <  1:5125648/151‑1 (MQ=255)
                                                                          cGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGa                                                                           >  2:138496/1‑151 (MQ=255)
                                                                            gCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGc                                                                         <  1:197978/151‑1 (MQ=255)
                                                                            gCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGc                                                                         >  1:2723373/1‑151 (MQ=255)
                                                                             ccAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGTCGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCCTTCTCGACTTGGGCCTGCTGGCTGTCGTCTGTGGGAGcc                                                                        >  2:2393239/1‑151 (MQ=255)
                                                                              cAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCa                                                                       <  2:792559/151‑1 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      >  2:3417617/1‑151 (MQ=255)
                                                                                ggCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGCGGGGTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACt                                                                     >  1:3153008/1‑151 (MQ=255)
                                                                                  cTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGt                                                                   >  1:4533397/1‑151 (MQ=255)
                                                                                   tGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTc                                                                  >  1:4561789/1‑151 (MQ=255)
                                                                                    gCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCt                                                                 >  1:4848521/1‑151 (MQ=255)
                                                                                     ccGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCtt                                                                <  1:284692/151‑1 (MQ=255)
                                                                                     ccGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCtt                                                                >  2:1139008/1‑151 (MQ=255)
                                                                                        cTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGcc                                                             >  2:509712/1‑150 (MQ=255)
                                                                                         tGGCGATGATTGCCAGGCGGCGACTGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTc                                                            >  1:1087055/1‑151 (MQ=255)
                                                                                         tGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTc                                                            >  2:4730981/1‑151 (MQ=255)
                                                                                              atgatTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGAttt                                                       <  1:393978/151‑1 (MQ=255)
                                                                                               tgatTGCCGGGCGGCGACGGGTTTTCGCGCTCGGCCTCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTc                                                      <  2:3088048/151‑1 (MQ=255)
                                                                                                   tGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTaaa                                                  >  2:4305179/1‑151 (MQ=255)
                                                                                                   tGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTaaa                                                  >  1:2886357/1‑151 (MQ=255)
                                                                                                       gggcggcGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCt                                              <  2:3480571/150‑1 (MQ=255)
                                                                                                           ggcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgcg                                          <  1:446048/151‑1 (MQ=255)
                                                                                                            gcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgcgc                                         <  1:2036963/151‑1 (MQ=255)
                                                                                                                 gggTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGAGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCGGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATcc                                    >  1:2746093/1‑151 (MQ=255)
                                                                                                                    ttttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtg                                 >  1:1279821/1‑151 (MQ=255)
                                                                                                                    ttttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtg                                 >  2:4519455/1‑151 (MQ=255)
                                                                                                                    ttttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtg                                 <  1:214584/151‑1 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTgg                              >  1:1899401/1‑151 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTgg                              >  2:1517996/1‑151 (MQ=255)
                                                                                                                         gcgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGa                            <  1:957851/151‑1 (MQ=255)
                                                                                                                          ggcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAg                           <  2:1611422/150‑1 (MQ=255)
                                                                                                                            cTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCg                         <  2:3564337/151‑1 (MQ=255)
                                                                                                                             tCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCgg                        >  1:4275644/1‑151 (MQ=255)
                                                                                                                             tCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCgg                        <  1:3198351/151‑1 (MQ=255)
                                                                                                                                  cGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTg                   >  2:660272/1‑151 (MQ=255)
                                                                                                                                  cGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTg                   >  2:1809320/1‑151 (MQ=255)
                                                                                                                                   gCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGc                  >  1:3011467/1‑151 (MQ=255)
                                                                                                                                   gCTGTTCAGTTTCGGCGGGCGAGGGTTTGTCTACGGCAACATTCATGGATGGTTTCCATTCTCGAATTGGGGCTGCTGGCTGTCGGGTGTGGGAGCCCCTGTCTTGCTCGATATCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGcgggc                  >  2:2865851/1‑147 (MQ=255)
                                                                                                                                      gTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcg               >  1:1773762/1‑151 (MQ=255)
                                                                                                                                       ttCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcgc              >  2:3020862/1‑151 (MQ=255)
                                                                                                                                       ttCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcgc              <  1:1375133/151‑1 (MQ=255)
                                                                                                                                         cAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGa            <  2:4063484/151‑1 (MQ=255)
                                                                                                                                            tttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAaca         <  1:2507503/151‑1 (MQ=255)
                                                                                                                                            tttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAaca         <  1:5011771/151‑1 (MQ=255)
                                                                                                                                                   gggtgAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGGTTTCTAAAAGCTGGCGCGAACCGTGTTGGAGCGGGGTTTCCCCGGGACACgggggg  >  2:523198/1‑148 (MQ=255)
                                                                                                                                                   gggtgAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGa  >  2:3940755/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑