Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 542,073 T→C intergenic (‑334/+195) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974542,0730TC94.3% 167.7 / 1.7 74intergenic (‑334/+195)argD/RPPX_02300acetylornithine aminotransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base C (6/60);  total (6/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCG  >  CP009974/541928‑542199
                                                                                                                                                 |                                                                                                                              
ggTGAGCGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgc                                                                                                                           <  2:4276284/151‑1 (MQ=255)
ggTGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgc                                                                                                                           <  1:2688496/151‑1 (MQ=255)
ggTGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgc                                                                                                                           <  1:2687468/151‑1 (MQ=255)
 gTGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcg                                                                                                                          <  1:2727420/151‑1 (MQ=255)
  tGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgc                                                                                                                         <  2:1722335/151‑1 (MQ=255)
  tGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgc                                                                                                                         <  1:4961853/151‑1 (MQ=255)
  tGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgc                                                                                                                         <  1:2099631/151‑1 (MQ=255)
   gAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgcg                                                                                                                        <  2:3541887/151‑1 (MQ=255)
   gAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgcg                                                                                                                        <  1:3439577/151‑1 (MQ=255)
   gAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgcg                                                                                                                        <  2:854606/151‑1 (MQ=255)
    aaCGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgcgc                                                                                                                       <  1:1060981/151‑1 (MQ=255)
    aaCGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGgcgcgcgc                                                                                                                       >  1:3608867/1‑151 (MQ=255)
     aCGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGcgcgcgcg                                                                                                                      <  2:2614568/151‑1 (MQ=255)
     aCGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGcgcgcgcg                                                                                                                      <  1:2908044/151‑1 (MQ=255)
       gggTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGAc                                                                                                                    <  2:1847191/151‑1 (MQ=255)
       gggTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGAc                                                                                                                    <  1:190522/151‑1 (MQ=255)
        ggTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGAcc                                                                                                                   <  1:691014/151‑1 (MQ=255)
           gtTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGcc                                                                                                                <  2:4410536/151‑1 (MQ=255)
             tCAAACAGGAGTGGGGGGGGACAGGATGGACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCtt                                                                                                              <  2:2600181/151‑1 (MQ=18)
              cacacaGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCttg                                                                                                             >  1:4356629/1‑151 (MQ=255)
                    ggATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCttgtttgtt                                                                                                       <  2:659216/151‑2 (MQ=255)
                     gATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCttgtttgttc                                                                                                      <  1:1487488/151‑3 (MQ=255)
                     gATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCttgtttgttc                                                                                                      <  1:4969567/151‑3 (MQ=255)
                          tgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgacgg                                                                                                 <  1:2223392/151‑2 (MQ=18)
                          tgAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTtcgacgg                                                                                                 <  1:1662714/151‑2 (MQ=18)
                            ggggaACAGGATGTACGTCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGa                                                                                               <  1:3220001/150‑1 (MQ=17)
                             ggggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGat                                                                                              <  1:4729072/151‑1 (MQ=18)
                             ggggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGat                                                                                              <  1:4729145/151‑1 (MQ=18)
                              gggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGata                                                                                             >  2:2266883/1‑151 (MQ=18)
                              gggACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGata                                                                                             <  1:1983385/151‑1 (MQ=18)
                                gACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATc                                                                                           <  2:5536/151‑1 (MQ=18)
                                gACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATc                                                                                           <  1:1487643/151‑1 (MQ=18)
                                gACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATc                                                                                           <  1:1487792/151‑1 (MQ=18)
                                   aGGATGTACGGCGATGTTGATGTGTTGGCGGGTAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAg                                                                                        <  2:4424162/151‑1 (MQ=17)
                                    tcttGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGCGCGCCGCCCGCGCGGCGCTTCGCGGGTAAGCCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                       <  1:1845468/148‑1 (MQ=17)
                                    ggATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                       >  1:2994349/1‑151 (MQ=17)
                                    ggATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAgg                                                                                       <  1:2266883/151‑1 (MQ=17)
                                     gctGTACGGTGATGTTGATGTGTGCGCGCGGAGACCGCGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCGTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggt                                                                                      <  2:4875417/149‑2 (MQ=14)
                                     gATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggt                                                                                      <  1:3356929/151‑2 (MQ=17)
                                       tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGGCGGGATATCGAggtgt                                                                                    >  2:1263122/1‑148 (MQ=17)
                                       tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgt                                                                                    <  2:1955803/151‑4 (MQ=17)
                                       tGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCCCCTGCGCCCGGCGCGCGCGACCGCCTCGTCTGTTCGACGGGAGATCGAggaga                                                                                    >  2:1845468/1‑148 (MQ=17)
                                        gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                   <  1:1423709/151‑5 (MQ=17)
                                        gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                   <  1:4728859/151‑5 (MQ=17)
                                        gTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtg                                                                                   <  2:3779377/151‑5 (MQ=17)
                                          aCGGGGATGTTGATGTGTTGGCGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTgcg                                                                                 <  1:4474010/151‑1 (MQ=14)
                                          aCGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAggtgtgcg                                                                                 <  2:4356629/151‑7 (MQ=17)
                                                aTGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAgg                                                                           <  1:3232601/151‑1 (MQ=14)
                                                 ggTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGc                                                                          <  1:4568596/150‑1 (MQ=14)
                                                  gTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCg                                                                         <  1:1218108/151‑1 (MQ=14)
                                                   ttGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGt                                                                        <  1:4544614/151‑1 (MQ=14)
                                                    tGATGTGTTGGCGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                       <  2:131273/151‑1 (MQ=14)
                                                    tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                       <  1:1669614/151‑1 (MQ=14)
                                                    tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                       <  2:1230604/151‑1 (MQ=14)
                                                    tGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGtt                                                                       <  2:3693187/151‑1 (MQ=14)
                                                     gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                      <  1:1263122/151‑1 (MQ=14)
                                                     gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                      <  1:1816659/151‑1 (MQ=14)
                                                     gATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTc                                                                      <  1:3659702/151‑1 (MQ=14)
                                                      aTGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcg                                                                     <  2:3608867/151‑1 (MQ=14)
                                                       tgtgTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgc                                                                    <  2:2994349/151‑1 (MQ=14)
                                                         tgtTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTcgcgc                                                                  <  2:1928986/151‑1 (MQ=11)
                                                               ccgggAGATCGGCCGCCGCCCGCGTGGCGCTTCGCGGGTAGACCCTCTCCTACGCTTGTTTCGGGCCAATCCCTCCTGCGCCAGGCGCGCGCGACCGCCATGTATGTTCGACGGGAGATCGAGGTGTGCGGCAAGGCGTTCGCGCGCAAAt                                                            <  2:4015087/149‑1 (MQ=11)
                                                               cGGGGAGATCGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAAt                                                            <  2:2667824/151‑1 (MQ=11)
                                                                ggggAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGCTTGTTTCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATg                                                           <  1:2574871/151‑1 (MQ=11)
                                                                     gagcGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCcaca                                                      <  1:1991167/148‑1 (MQ=0)
                                                                        cgggcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAGGc                                                   <  2:1604987/148‑1 (MQ=0)
                                                                                   cgagcggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGCACCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGccc                                        <  1:4205026/148‑1 (MQ=1)
                                                                                            ctgcgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGCACCAGTCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGCCCGaaacaaac                               <  1:3185244/148‑1 (MQ=1)
                                                                                                            gcgccTGCGTTTGTTTCGGGCCAGTCCCTCCTGCACCGGTCGCGCGCGCCCGCCTTGTTTGTACGACGCGATATCGAGACATGCGCCAAGGCGGTCGCGCGCAAATCCCACAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTAc               <  2:1922187/148‑1 (MQ=1)
                                                                                                                   cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa        <  1:4127392/148‑1 (MQ=1)
                                                                                                                   cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa        <  1:4127480/148‑1 (MQ=1)
                                                                                                                   cgcttgtttCGGGCCAGTCCCTCCTGCGCCCGGCGCGCGCGACCGCCTTGTTTGTTCGACGGGATATCGAGGTGTGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCAGGTTTACCCGCGaa        <  2:3981971/148‑1 (MQ=1)
                                                                                                                    gtgtgtttCGGGCCAGTCCATCCTGTGCCTGGCGCGCGCGACCGCCTTTTTTGTACGACGCAATATCGAGGCATGCACCAAGGCGTTCGCGCGCAAATCCCACAAGCATAATTGGCCTGAAACAAGCGTAGGAGCGGGTTTACCCGCGAAg       <  1:1796067/148‑1 (MQ=1)
                                                                                                                         ttgcGGGCCAGGCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTGGTACGACGCGATATCGAGACAGGCACCAAGGCGTTCGCGCGCGAATCCCACAGGCATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccg  <  1:4614198/148‑1 (MQ=1)
                                                                                                                                                 |                                                                                                                              
GGTGAACGGGTGTTCACACAGGATTGTGAGGGGACAGGATGTACGGCGATGTTGATGTGTTGGCGGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTCCCTCCTGTGCCTGGCGCGCGCGACCGCCTTGTTTGTACGACGCGATATCGAGGCATGCGCCAAGGCGTTCGCGCGCAAATGCCACAGGCATAACTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCG  >  CP009974/541928‑542199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: