Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,147,209	A→G	intergenic (+461/‑560)	RPPX_18425 → / → RPPX_18430	enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,147,209	0	A	G	100.0%	68.9 / NA	44	intergenic (+461/‑560)	RPPX_18425/RPPX_18430	enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

GGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTC  >  CP009974/4147068‑4147246                                                                                                                                                     |                                      ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                     <  1:4941841/151‑1 (MQ=11)  gCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTg                                    >  2:2268442/1‑151 (MQ=11)   cGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTgg                                   >  2:3176323/1‑151 (MQ=11)    gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                  <  1:2172156/151‑1 (MQ=11)    gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                  >  1:285785/1‑151 (MQ=11)    gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                  >  2:1840035/1‑151 (MQ=11)     aaaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcg                                 <  2:1145600/151‑1 (MQ=11)      aaaGGTGAGTCAGCGTCGCCATCGCAAGTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                >  2:4071716/1‑151 (MQ=11)      aaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                >  1:2603871/1‑151 (MQ=11)      aaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                >  2:448572/1‑151 (MQ=11)       aaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCa                               >  1:1261695/1‑151 (MQ=11)         ggTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATa                             <  2:5058405/151‑1 (MQ=11)          gTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAg                            >  2:4743152/1‑151 (MQ=11)           tGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGt                           <  2:1161906/151‑1 (MQ=11)           tGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGt                           <  1:4721095/151‑1 (MQ=11)            gAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTc                          <  2:40469/151‑1 (MQ=11)            gAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTc                          <  2:3092968/151‑1 (MQ=11)              gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCCTAGTCAc                        >  2:404306/1‑151 (MQ=11)              gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAc                        <  2:5170870/151‑1 (MQ=11)              gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAc                        <  2:3801178/151‑1 (MQ=11)              gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAc                        <  2:2196851/151‑1 (MQ=11)               tCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAcc                       <  2:2190766/151‑1 (MQ=11)                 aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtt                     >  1:3903814/1‑151 (MQ=11)                 aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtt                     >  2:104930/1‑151 (MQ=11)                 aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtt                     >  1:581185/1‑151 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                   >  1:4072577/1‑151 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                   >  1:1333256/1‑151 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtttac                  <  2:1754817/151‑3 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                  <  2:1754818/151‑1 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                  <  1:873288/151‑1 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTAGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                  >  1:3070718/1‑151 (MQ=11)                     tCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCg                 >  2:1249817/1‑151 (MQ=11)                     tCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCg                 >  2:4123263/1‑151 (MQ=11)                      cGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGc                <  1:1021427/151‑1 (MQ=11)                      cGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGc                >  1:7982/1‑151 (MQ=11)                        ccATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGATTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGccc              <  2:2603737/151‑1 (MQ=11)                         cATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCt             >  1:2919498/1‑151 (MQ=11)                         cATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCt             >  1:2247284/1‑151 (MQ=11)                           tCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa           <  2:189014/151‑1 (MQ=11)                           tCGCAAATGGACTCTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa           <  1:5046223/151‑1 (MQ=11)                               aaaTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggc       >  1:3363215/1‑151 (MQ=11)                                 aTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggcgg     <  1:2832746/151‑1 (MQ=11)                                  tGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCggg    <  2:7982/151‑1 (MQ=11)                                    gACTGTTCACTGAGCCAAAACAGACGAGTTCACTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTAGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTc  <  1:447663/151‑1 (MQ=11)                                                                                                                                                     |                                      GGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTC  >  CP009974/4147068‑4147246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑