| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009974 | 4,165,105 | G→A | intergenic (‑299/‑176) | RPPX_18525 ← / → RPPX_18530 | transporter/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009974 | 4,165,105 | 0 | G | A | 96.1% | 119.3 / ‑1.9 | 51 | intergenic (‑299/‑176) | RPPX_18525/RPPX_18530 | transporter/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (25/24); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
ACTCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAGGTTCTTAACCAAACGCTCTTTAACAAATCGAATCAAGCAATTC > CP009974/4164961‑4165148 | acTCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCtt < 1:5169924/151‑1 (MQ=255) cTCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTa > 1:3923660/1‑151 (MQ=255) tCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTaa < 2:402351/151‑1 (MQ=255) tCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTaa > 2:1397871/1‑151 (MQ=255) tCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTaa > 2:1397648/1‑151 (MQ=255) accAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAAAAACAAAAAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTaa < 2:402196/150‑1 (MQ=17) ccAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAAc > 1:3129836/1‑151 (MQ=255) ccAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAAc < 1:3611675/151‑1 (MQ=255) aCGCGGTAGCGAAGCAAAGCTAGCCACTGTATTTAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaa > 2:1248455/1‑151 (MQ=255) aCGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaa < 2:2240074/151‑1 (MQ=255) cgcgGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaac < 1:4411707/151‑2 (MQ=255) cgcgGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaac < 1:3934365/151‑2 (MQ=255) gcgGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaacg < 2:3534747/151‑3 (MQ=255) cgGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaacgc > 1:1197275/1‑148 (MQ=255) ggTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaacgct > 2:718664/1‑147 (MQ=255) ggTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaacgct > 1:2020886/1‑147 (MQ=255) ggTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaacgct < 1:2323275/151‑5 (MQ=17) ggTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGATTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACCaacgct > 2:1107594/1‑147 (MQ=17) gTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGctc > 1:3103275/1‑151 (MQ=17) aGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCtt < 1:2919467/151‑1 (MQ=14) cGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGTATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTa > 2:4565996/1‑151 (MQ=14) cGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTa > 1:3099084/1‑151 (MQ=14) cGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTa < 1:4542838/151‑1 (MQ=14) gAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTaa > 2:2757666/1‑151 (MQ=14) aaGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAAc < 1:4871865/151‑1 (MQ=14) aGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACa < 2:1969329/151‑1 (MQ=14) gCAAAGGTAGCCACTGGATTGAGTGAAGTGTGTGAAGCTAAGCGAGTTTCTCGCAAAAAACTGCAAGATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACaa < 2:5211946/151‑1 (MQ=11) gCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACaa < 2:2010689/151‑1 (MQ=14) cAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACaaa > 1:1754546/1‑151 (MQ=14) cAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACaaa < 1:1711507/151‑1 (MQ=14) aaaGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAAt > 1:817184/1‑151 (MQ=14) aaGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATc < 2:5091906/151‑1 (MQ=14) aaGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATc > 2:1029447/1‑151 (MQ=14) aGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCg < 2:2010591/151‑1 (MQ=11) aGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCg < 1:2123667/151‑1 (MQ=11) aGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCg < 1:3403971/151‑1 (MQ=14) aGCTAGCAACTGTATTGAGTAAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCg < 2:4279832/151‑1 (MQ=11) gCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGa < 1:3726740/151‑1 (MQ=11) gCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGa > 2:714126/1‑151 (MQ=11) cTAGCCACTGTATAGAGACAAGAGATTGAAGCTAAACGAGTTTCTAGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGaa > 2:3577941/1‑151 (MQ=11) tAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAAt > 1:915195/1‑151 (MQ=11) tAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAAt > 1:636292/1‑151 (MQ=11) tAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAAt > 1:2612827/1‑151 (MQ=11) aGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAATc < 2:4453255/151‑1 (MQ=11) gCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAATCa < 2:3746211/151‑1 (MQ=11) aCTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAATCAAGc > 1:1199721/1‑151 (MQ=11) tGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAATCAAGCaa > 2:4758951/1‑151 (MQ=11) tGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCGCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAATCAAGCaa > 2:246453/1‑151 (MQ=11) gaagcgAGTTAAGTGACTGAAGTTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAGGTTCTTAACCAAACGCTCTTTAACAAATCGAATCAAGCaa > 2:4206179/6‑151 (MQ=1) tATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGATGAAAAGCTCTTAACC‑AACGCTCTTTAACAAATCGAATCAAGCAAtt > 1:1367519/1‑151 (MQ=255) gcgAGTTAAGTGACTGAAGTTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAGGTTCTTAACCAAACGCTCTTTAACAAATCGAATCAAGCAATTc < 1:3624635/149‑1 (MQ=11) | ACTCCAACGCGGTAGCGAAGCAAAGCTAGCCACTGTATTGAGTCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAGGTTCTTAACCAAACGCTCTTTAACAAATCGAATCAAGCAATTC > CP009974/4164961‑4165148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |