BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,170,224	T→C	noncoding (2917/2917 nt)	RPPX_18545 →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,170,224	0	T	C	98.4%	642.7 / ‑2.2	185	noncoding (2917/2917 nt)	RPPX_18545	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (81/101); total (81/104)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATTTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGA  >  CP009974/4170121‑4170369

  aaGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAg                                                                                                  >  2:1413756/1‑151 (MQ=14)

     aCTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCTAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGAcc                                                                                               >  1:3260617/1‑151 (MQ=16)

        acgacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACcaca                                                                                            <  1:3557295/151‑1 (MQ=25)

         cgacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACaa                                                                                           <  2:1104288/151‑1 (MQ=37)

         cgacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACaa                                                                                           >  2:479801/1‑151 (MQ=37)

          gacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACaaa                                                                                          <  1:108606/151‑1 (MQ=38)

          gacgTTGATAGGGTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACaaa                                                                                          <  2:3536720/151‑1 (MQ=255)

           acgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACaaaa                                                                                         >  2:4355492/1‑150 (MQ=255)

            cgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACCAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCCGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAACTTCGTAAGACCACAAata                                                                                        >  1:1269884/1‑151 (MQ=255)

               tGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCg                                                                                     <  2:1659848/151‑1 (MQ=255)

                 aTAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCa                                                                                   <  1:3380995/151‑1 (MQ=255)

                   ggggtGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATGGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCata                                                                                 <  2:3827273/147‑1 (MQ=255)

                    ggTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCatat                                                                                <  1:2262140/151‑1 (MQ=255)

                      ttGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATcc                                                                              <  1:2298445/151‑1 (MQ=255)

                       tGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACTCGAAAATTTGTAAGACCACAAATATCGCATATCCg                                                                             >  1:2996503/1‑151 (MQ=255)

                       tGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCg                                                                             <  2:3471780/151‑1 (MQ=255)

                        gggTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGa                                                                            <  2:3231948/151‑1 (MQ=255)

                         ggTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGaa                                                                           <  1:975234/151‑1 (MQ=255)

                           tgtgtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAAtt                                                                         <  1:820217/151‑1 (MQ=255)

                           tgtgtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAAtt                                                                         >  1:1569448/1‑151 (MQ=255)

                            gtgtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACGCCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTc                                                                        <  2:5010984/151‑1 (MQ=255)

                            gtgtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTc                                                                        <  1:4738736/151‑1 (MQ=255)

                              gtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGc                                                                      <  2:4686180/151‑1 (MQ=255)

                              gtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGc                                                                      <  2:1559219/151‑1 (MQ=255)

                                aaGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTg                                                                    >  2:605553/1‑151 (MQ=255)

                                   cgcTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGc                                                                 >  1:3262094/1‑151 (MQ=255)

                                   cgcTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGc                                                                 >  2:2754978/1‑151 (MQ=255)

                                    gcTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCt                                                                >  1:660314/1‑151 (MQ=255)

                                    ccTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAGCACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCt                                                                <  1:1885118/150‑1 (MQ=255)

                                      tgtgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGt                                                              <  1:4456118/151‑1 (MQ=255)

                                        tgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTcc                                                            >  2:1886019/1‑151 (MQ=255)

                                          aGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCAt                                                          <  2:2268125/151‑1 (MQ=255)

                                           ggCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATc                                                         >  1:4489740/1‑151 (MQ=255)

                                             cGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTg                                                       >  1:3819628/1‑151 (MQ=255)

                                              gTTGGGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTgg                                                      <  2:2495542/151‑1 (MQ=255)

                                              gTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTgg                                                      <  2:3046823/151‑1 (MQ=255)

                                              gTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTgg                                                      <  2:1132161/151‑1 (MQ=255)

                                              gTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTgg                                                      >  2:3991233/1‑151 (MQ=255)

                                              gTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTgg                                                      <  2:3459477/151‑1 (MQ=255)

                                              gTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTgg                                                      >  1:4359374/1‑151 (MQ=255)

                                               ttGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGa                                                     <  1:2735391/151‑1 (MQ=255)

                                               ttGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGa                                                     <  1:1020467/151‑1 (MQ=255)

                                                tGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATCACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAGTTCGTAAGCCCACAACTATCGCATATCCGAGTTCGCTGGGCTGTCCATCTGGAc                                                    <  2:786038/151‑1 (MQ=18)

                                                tGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGAc                                                    <  1:5145696/151‑1 (MQ=255)

                                                tGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGAc                                                    <  2:4471688/151‑1 (MQ=255)

                                                  aGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAt                                                  <  2:3987338/151‑1 (MQ=255)

                                                  aGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAt                                                  <  2:864072/151‑1 (MQ=255)

                                                   gCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAtt                                                 <  1:3312482/151‑1 (MQ=255)

                                                   gCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAtt                                                 >  1:4615010/1‑151 (MQ=255)

                                                   gCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAtt                                                 <  1:2626979/151‑1 (MQ=255)

                                                     tAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCt                                               >  2:2337691/1‑151 (MQ=255)

                                                     tAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCt                                               <  2:4726156/151‑1 (MQ=255)

                                                      aaCCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTg                                              <  1:967027/151‑1 (MQ=255)

                                                      aaCCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTg                                              <  2:2120918/151‑1 (MQ=255)

                                                         cAATACTAATTGCCCGTTAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCa                                           <  2:147657/151‑1 (MQ=255)

                                                         cAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCa                                           <  2:1342299/151‑1 (MQ=255)

                                                          aaTACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCaa                                          <  1:4859014/151‑1 (MQ=255)

                                                          aaTACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAAATCGTAAGACCACAAATATCGCATATCCGCATTCACTGGGCTGTCCATCTGGACATTCTGGCaa                                          >  1:4165611/1‑151 (MQ=255)

                                                           aTACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAAc                                         >  1:3286432/1‑151 (MQ=255)

                                                           aTACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAAc                                         >  1:2074028/1‑151 (MQ=255)

                                                             aCTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAg                                       >  1:1061173/1‑151 (MQ=255)

                                                             aCTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAg                                       <  1:2541640/151‑1 (MQ=255)

                                                             aCTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAg                                       <  1:2540950/151‑1 (MQ=255)

                                                              cTAATTGCCCGTGAGGCTTGACCATATAACGCCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACcac                                                                                             >  2:3119349/1‑96 (MQ=255)

                                                              cTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGa                                      <  2:3453002/151‑1 (MQ=255)

                                                               tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGaa                                     >  2:1621957/1‑151 (MQ=255)

                                                                 aTTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAAtt                                   <  2:1928238/151‑1 (MQ=255)

                                                                 aTTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAAtt                                   <  2:1782106/151‑1 (MQ=255)

                                                                  ttGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAAttt                                  <  2:2252187/151‑1 (MQ=255)

                                                                    gCCCGTGAGGCTTGACCATATAACCCCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt                                <  2:1270821/151‑1 (MQ=255)

                                                                    gCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt                                >  1:5121223/1‑151 (MQ=255)

                                                                     cccGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCtt                               <  1:3341818/151‑1 (MQ=255)

                                                                      ccGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGCTTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTg                              >  1:3936424/1‑151 (MQ=255)

                                                                      ccGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTg                              <  2:5123466/151‑1 (MQ=255)

                                                                      ccGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTg                              >  1:349541/1‑151 (MQ=255)

                                                                        gTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTgac                            >  2:4141015/1‑151 (MQ=255)

                                                                        gTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTgac                            >  2:2055502/1‑151 (MQ=255)

                                                                          gAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTgacga                          >  2:5159974/1‑151 (MQ=255)

                                                                           aGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTgacgac                         >  1:2467678/1‑151 (MQ=255)

                                                                             gCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCa                       <  2:3687093/151‑1 (MQ=255)

                                                                              cTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCAt                      <  2:5148190/151‑1 (MQ=255)

                                                                               ttGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATa                     >  2:688582/1‑151 (MQ=255)

                                                                               ttGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATa                     >  1:2965117/1‑151 (MQ=255)

                                                                               ttGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATa                     >  2:1262012/1‑151 (MQ=255)

                                                                                tGACCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATag                    <  2:5164013/151‑1 (MQ=255)

                                                                                  aCCATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATagag                  >  1:4428856/1‑151 (MQ=255)

                                                                                   ccATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCa                                           <  1:5217948/125‑1 (MQ=255)

                                                                                   ccATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCa                                           >  2:5217948/1‑125 (MQ=255)

                                                                                    cATATAACAGCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCa                <  2:2567876/151‑1 (MQ=255)

                                                                                    cATATAACACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCa                <  1:1474185/151‑1 (MQ=255)

                                                                                         aaCACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGa           <  2:4615010/151‑1 (MQ=255)

                                                                                         aaCACCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGa           <  2:2918174/151‑1 (MQ=255)

                                                                                          acacCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGaa          >  1:4538636/1‑151 (MQ=255)

                                                                                            acCCAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAacc        >  2:1563593/1‑151 (MQ=255)

                                                                                             cccAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAacca       <  1:1437104/151‑1 (MQ=255)

                                                                                               cAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAaccacc     <  2:4685701/151‑1 (MQ=255)

                                                                                               cAAGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAaccacc     >  1:1844293/1‑151 (MQ=255)

                                                                                                aaGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCTAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCt    <  2:1748577/151‑1 (MQ=255)

                                                                                                 aGCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTg   <  1:3121325/151‑1 (MQ=255)

                                                                                                  gCAATCTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGa  >  2:2444644/1‑151 (MQ=255)

CGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATTTGCTACGCAGATTGTGGTGGTGAAGACGAAAGACCCGAAAATTCGTAAGACCACAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGA  >  CP009974/4170121‑4170369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`