Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,204,859 C→T R398C (CGT→TGT)  RPPX_18715 → trigger factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,204,8590CT99.6% 993.7 / NA 262R398C (CGT→TGT) RPPX_18715trigger factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (134/126);  total (135/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

GATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAACCTC  >  CP009974/4204711‑4205005
                                                                                                                                                    |                                                                                                                                                  
gATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAgtgtgt                                                                                                                                                  >  1:1884169/1‑151 (MQ=255)
  tCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTc                                                                                                                                                <  1:4673844/151‑1 (MQ=255)
  tCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTc                                                                                                                                                <  1:2169905/151‑1 (MQ=255)
   cGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCg                                                                                                                                               <  2:4258335/151‑1 (MQ=255)
   cGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCg                                                                                                                                               <  1:5211079/151‑1 (MQ=255)
   cGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCg                                                                                                                                               <  1:592608/151‑1 (MQ=255)
   cGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCg                                                                                                                                               <  2:110894/151‑1 (MQ=255)
    gTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCgg                                                                                                                                              <  2:2484617/151‑1 (MQ=255)
      ggCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGtt                                                                                                                                            >  2:802047/1‑151 (MQ=255)
      ggCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGtt                                                                                                                                            >  1:72214/1‑151 (MQ=255)
       gCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTtg                                                                                                                                           >  1:2773468/1‑151 (MQ=255)
       gCCGAAGAGGTCAAGCAGTTCGAGCGGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCACCGCCTGGTACTAAAAGAACGACCAGCAACTGAACAGAGTGTGTTCgggag                                                                                                                                           >  2:2847755/1‑148 (MQ=255)
        ccGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTtgt                                                                                                                                          <  1:371487/151‑1 (MQ=255)
        ccGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTtgt                                                                                                                                          <  2:45037/151‑1 (MQ=255)
         cGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTtgtg                                                                                                                                         >  1:871671/1‑151 (MQ=255)
            aGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTg                                                                                                                                      >  2:715668/1‑151 (MQ=255)
              tGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGga                                                                                                                                    <  2:3694180/151‑1 (MQ=255)
                 tCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGgaaga                                                                                                                                 >  2:1246835/1‑151 (MQ=255)
                      cAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAg                                                                                                                            >  2:3611385/1‑151 (MQ=255)
                      cAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAg                                                                                                                            >  2:4399939/1‑151 (MQ=255)
                       aGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGt                                                                                                                           >  2:1386039/1‑151 (MQ=255)
                         ttCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTg                                                                                                                         >  1:1231923/1‑151 (MQ=255)
                         ttCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTg                                                                                                                         <  1:3425631/151‑1 (MQ=255)
                         ttCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGCGCGTTCGGTTGTGCTGGAAGAACAAGCTg                                                                                                                         >  2:2165345/1‑151 (MQ=255)
                            gAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAg                                                                                                                      >  2:1325912/1‑151 (MQ=255)
                            gAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAg                                                                                                                      >  1:4335749/1‑151 (MQ=255)
                                 gAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACt                                                                                                                 >  1:4131801/1‑151 (MQ=255)
                                   aGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGt                                                                                                               <  1:3949804/151‑1 (MQ=255)
                                       ggACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTg                                                                                                           <  2:2679804/151‑1 (MQ=255)
                                        gacgacGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGc                                                                                                          <  2:1173774/151‑1 (MQ=255)
                                          cgacgCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAg                                                                                                        >  2:3215976/1‑151 (MQ=255)
                                           gacgCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGa                                                                                                       >  1:4906018/1‑151 (MQ=255)
                                            acgCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGaa                                                                                                      <  1:450674/151‑1 (MQ=255)
                                                 aaGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGa                                                                                                 >  1:234017/1‑151 (MQ=255)
                                                  aGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGAc                                                                                                <  1:2617232/151‑1 (MQ=255)
                                                       cgcgAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGa                                                                                           <  2:3830842/151‑1 (MQ=255)
                                                       cgcgAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGa                                                                                           <  2:2023015/151‑1 (MQ=255)
                                                        gcgAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGAc                                                                                          <  1:1304728/151‑1 (MQ=255)
                                                         cgAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGAcc                                                                                         <  2:5182686/151‑1 (MQ=255)
                                                          gagaTGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCg                                                                                        <  2:2094212/151‑1 (MQ=255)
                                                              tgatCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACaa                                                                                    <  2:189150/151‑1 (MQ=255)
                                                              tgatCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACaa                                                                                    >  2:186644/1‑151 (MQ=255)
                                                               gatCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAg                                                                                   >  2:1610941/1‑151 (MQ=255)
                                                               gatCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAg                                                                                   >  2:3406943/1‑151 (MQ=255)
                                                                atCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGt                                                                                  >  2:577413/1‑151 (MQ=255)
                                                                 tCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTc                                                                                 >  1:2241165/1‑151 (MQ=255)
                                                                   gaagaaATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCgg                                                                               >  1:2129395/1‑151 (MQ=255)
                                                                   gaagaaATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCgg                                                                               >  1:2490568/1‑151 (MQ=255)
                                                                    aagaaATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGt                                                                              >  1:5101928/1‑151 (MQ=255)
                                                                      gaaATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGtct                                                                            >  2:107152/1‑151 (MQ=255)
                                                                          tGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTa                                                                        >  1:3363102/1‑151 (MQ=255)
                                                                          gggCTTCGGCTTACGAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTa                                                                        <  2:1762585/150‑1 (MQ=255)
                                                                           ggCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTAc                                                                       <  2:2380458/151‑1 (MQ=255)
                                                                           ggCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTAc                                                                       >  2:4155944/1‑151 (MQ=255)
                                                                              ttCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACgaa                                                                    >  1:2724249/1‑151 (MQ=255)
                                                                                 ggCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACgaagaa                                                                 >  1:3330215/1‑151 (MQ=255)
                                                                                 ggCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACgaagaa                                                                 >  1:5238788/1‑151 (MQ=255)
                                                                                   cTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGc                                                               >  2:5128155/1‑151 (MQ=255)
                                                                                      aCCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGt                                                            >  2:312281/1‑151 (MQ=255)
                                                                                           gagCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAAc                                                       <  1:4293612/151‑1 (MQ=255)
                                                                                            agCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAAcc                                                      <  1:1140245/151‑1 (MQ=255)
                                                                                              ccTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAg                                                    >  1:3631918/1‑151 (MQ=255)
                                                                                              ccTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAg                                                    >  2:4884666/1‑151 (MQ=255)
                                                                                                tGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGcc                                                  <  1:497221/151‑1 (MQ=255)
                                                                                                 gAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCg                                                 >  1:1905878/1‑151 (MQ=255)
                                                                                                   gCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAg                                               <  1:2304856/151‑1 (MQ=255)
                                                                                                     aGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGc                                             >  1:1383052/1‑151 (MQ=255)
                                                                                                            cGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGcc                                      <  2:4624403/151‑1 (MQ=255)
                                                                                                             gCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCg                                     >  2:934365/1‑151 (MQ=255)
                                                                                                             gCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCg                                     <  2:3057914/151‑1 (MQ=255)
                                                                                                                tGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCgctg                                  >  1:2046822/1‑151 (MQ=255)
                                                                                                                tGGTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGGCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCgctg                                  >  1:1745895/1‑151 (MQ=255)
                                                                                                                  gTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCgctgct                                >  1:104993/1‑151 (MQ=255)
                                                                                                                  gTACTACAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCgctgct                                >  1:4826695/1‑151 (MQ=255)
                                                                                                                    actacAAGAACGATCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGa                              <  1:5040184/151‑1 (MQ=255)
                                                                                                                      tacAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGTCTCCTGCCGCTGCTGAGt                            >  1:3004245/1‑151 (MQ=255)
                                                                                                                        cAAGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGCTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGtca                          >  2:3968630/1‑149 (MQ=255)
                                                                                                                         aaGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAAc                         >  2:2708018/1‑151 (MQ=255)
                                                                                                                         aaGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGATCCTGCCGCTGCTGAGTAAc                         >  2:3533629/1‑151 (MQ=255)
                                                                                                                          aGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACg                        <  2:2998585/151‑1 (MQ=255)
                                                                                                                          aGAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACg                        <  1:4210194/151‑1 (MQ=255)
                                                                                                                           gAACGACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACgg                       <  1:1003156/151‑1 (MQ=255)
                                                                                                                               gACCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCtt                   <  2:1957077/151‑1 (MQ=255)
                                                                                                                                aCCAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTtc                  >  2:1751164/1‑151 (MQ=255)
                                                                                                                                 ccAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTtct                 <  2:3462957/151‑1 (MQ=255)
                                                                                                                                 ccAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTtct                 >  1:1946612/1‑151 (MQ=255)
                                                                                                                                  cAGCAACTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTtctc                <  2:4493708/151‑1 (MQ=255)
                                                                                                                                      aaCTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGt            <  1:2003275/151‑1 (MQ=255)
                                                                                                                                      aaCTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGt            >  1:1612133/1‑151 (MQ=255)
                                                                                                                                       aCTGAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTa           >  1:3203131/1‑151 (MQ=255)
                                                                                                                                          gAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCt                    >  1:3850070/1‑139 (MQ=255)
                                                                                                                                          gAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGa        >  1:5216590/1‑151 (MQ=255)
                                                                                                                                          gAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGa        >  2:1818380/1‑151 (MQ=255)
                                                                                                                                          gAACGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCCCGTAGGa        >  2:1398582/1‑151 (MQ=255)
                                                                                                                                           aaCGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGaa       >  1:4750624/1‑151 (MQ=255)
                                                                                                                                             cGAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAAc     >  2:4057140/1‑151 (MQ=255)
                                                                                                                                              gAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAAcc    <  1:2968094/151‑1 (MQ=255)
                                                                                                                                              gAAGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAAcc    >  2:3009064/1‑151 (MQ=255)
                                                                                                                                               aaGTGTGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAACCt   >  2:821421/1‑151 (MQ=255)
                                                                                                                                                aGTGGGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAACCTc  <  2:4766967/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAAGAGCCTGAGCAGGTCATCGCCTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCTCCTGCCGCTGCTGAGTAACGGGCTTCTCTCGTAGGAAACCTC  >  CP009974/4204711‑4205005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑