Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,537,108	A→G	Y105Y (TAT→TAC)	RPPX_20270 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,537,108	0	A	G	100.0%	234.5 / NA	73	Y105Y (TAT→TAC)	RPPX_20270	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/39);  total (34/39)

GTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTG  >  CP009974/4536960‑4537159                                                                                                                                                     |                                                    gtggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTa                                                   <  1:1078824/151‑1 (MQ=255) gtggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTa                                                   <  1:4046678/151‑1 (MQ=255) gtggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTa                                                   <  1:2845791/151‑1 (MQ=255)   ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 >  2:4887497/1‑151 (MQ=255)   ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 >  1:2824762/1‑151 (MQ=255)   ggcgCTGCGCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 <  2:2816483/151‑1 (MQ=255)    gcgcTGCTCGGACGTTGAACAGGCGGAAGACGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTg                                                <  2:545886/151‑1 (MQ=255)     ggctgctCGGACGTTGGACGGGCGGCCGATGCCACCCCAGGATAGGGCGCCGGTCAGGTTGTAGGGGACCAGCACGGCCCAGCACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCGCCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                               <  2:3105078/150‑1 (MQ=14)     cgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                               >  2:3312472/1‑151 (MQ=255)      gctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCCGGGGGCGTAGTGgc                                              >  2:4590230/1‑151 (MQ=255)      gctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgc                                              >  2:2319935/1‑151 (MQ=255)      gctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgc                                              >  1:912477/1‑151 (MQ=255)       ctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcg                                             <  1:2183014/151‑1 (MQ=255)        tgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcgc                                            >  2:1001715/1‑151 (MQ=255)         gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:2369905/1‑151 (MQ=255)         gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  2:4836348/1‑151 (MQ=255)          ctCGGACGTGGGAGAGGCGGCAGAGGCCAGGCCGGCAGGGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGGACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          <  2:2944417/151‑1 (MQ=14)           tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         >  2:2780223/1‑151 (MQ=255)             ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCCGGGGGCGTCGCGGCGCCAGGc                                       >  2:2544966/1‑151 (MQ=255)              gACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                      >  2:3088657/1‑151 (MQ=255)              gACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                      <  2:984776/151‑1 (MQ=255)               aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     <  1:2929322/151‑1 (MQ=255)               aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     <  2:4443340/151‑1 (MQ=255)                cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    >  1:393162/1‑151 (MQ=255)                cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    >  1:1510963/1‑151 (MQ=255)                 gtggAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGCTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTTGTAGCGCCAGGCTTCt                                   >  1:430335/4‑151 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGTCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  2:4149839/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  2:3875440/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  1:1308148/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   >  1:4542282/1‑151 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  1:3589410/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  2:4150157/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  2:1486276/151‑1 (MQ=255)                  ttGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTg                                  >  1:1217578/1‑151 (MQ=255)                    gAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGcc                                <  1:4255999/151‑1 (MQ=255)                    gAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGcc                                <  2:1047704/151‑1 (MQ=255)                    gAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGGAGTGGCGCCAGGCTCCTGcc                                >  2:4850253/1‑151 (MQ=255)                     aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                               >  1:118810/1‑151 (MQ=255)                     aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                               <  2:1022727/151‑1 (MQ=255)                     aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                               <  1:3552459/151‑1 (MQ=255)                     aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                               >  1:3156183/1‑151 (MQ=255)                       ctgacggcATATGCCATACCAGCAAAAGGCTACGGTGAGGGTGGAGGGGAACAGCACGTCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCTCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCggg                             <  1:4822524/147‑1 (MQ=11)                       cAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCggg                             >  1:818824/1‑151 (MQ=255)                       cAGGAGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCggg                             <  1:1154196/151‑1 (MQ=255)                        gggcggcACATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgggg                            <  2:4778453/150‑1 (MQ=255)                           cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         >  1:3214154/1‑149 (MQ=255)                            ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCggggcgag                        >  2:4194010/1‑147 (MQ=255)                              cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      >  1:1692946/1‑151 (MQ=255)                              cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      <  2:3906617/151‑1 (MQ=255)                              cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      <  1:549569/151‑1 (MQ=255)                               ggATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  2:3016599/150‑1 (MQ=255)                               aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  1:2407868/151‑1 (MQ=255)                               aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  2:3685055/151‑1 (MQ=255)                                gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  2:2069500/1‑151 (MQ=255)                                 aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   <  1:4094082/151‑1 (MQ=255)                                  tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  1:3326400/151‑1 (MQ=255)                                    ccATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATgg                >  1:3901960/1‑151 (MQ=255)                                    ccATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATgg                >  1:3901681/1‑151 (MQ=255)                                      aTACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGTCGGGGTGAGGCAGATGGCg              <  1:862081/151‑1 (MQ=255)                                       tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt             <  1:4001998/151‑1 (MQ=19)                                         ccAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGc           >  2:191091/1‑151 (MQ=21)                                          cagcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa          >  2:3711785/1‑151 (MQ=18)                                          cagcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa          <  1:191091/151‑1 (MQ=19)                                          cagcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa          >  1:2994071/1‑151 (MQ=19)                                           atcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAg         >  1:2879669/3‑151 (MQ=12)                                             cagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTg       >  2:2831209/1‑151 (MQ=17)                                              ggagGGCGACGGTCAGGTTGTGGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTGTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTgg      <  2:2663356/150‑1 (MQ=255)                                              agagGGCGACGGTCAGGTTGTAGGGGAACGGCGCGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTgg      <  2:2004258/151‑1 (MQ=9)                                               gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc     <  1:868553/151‑1 (MQ=17)                                               gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc     >  1:847188/1‑151 (MQ=17)                                                agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGAGTGAGGCAGATGGCGTGCAGTGGCt    >  1:2720625/1‑151 (MQ=12)                                                 gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt   <  2:139805/151‑1 (MQ=9)                                                  ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg  <  2:642921/151‑1 (MQ=2)                                                                                                                                                     |                                                    GTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTG  >  CP009974/4536960‑4537159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑