BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,543,770	A→G	A451A (GCT→GCC)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,543,770	0	A	G	99.3%	396.9 / NA	148	A451A (GCT→GCC)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (70/77); minor base C (1/0); total (71/77)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GCGCTGTGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGT  >  CP009974/4543622‑4543860

gcgcTGTGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGc                                                                                          <  2:1045985/151‑1 (MQ=255)

 cgcTGTGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGTACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGcc                                                                                         <  2:4034549/151‑1 (MQ=255)

 cgcTGTGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGcc                                                                                         <  2:2637671/151‑1 (MQ=255)

 cgcTGTGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGcc                                                                                         >  2:457575/1‑151 (MQ=255)

    tgtgGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGtg                                                                                      >  2:537647/1‑150 (MQ=255)

    tgtgGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGtg                                                                                      >  1:4464688/1‑150 (MQ=255)

    tgtgGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGtg                                                                                      <  1:2157398/151‑2 (MQ=255)

    tgtgGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGtg                                                                                      <  1:2157149/151‑2 (MQ=255)

       ggCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCGATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTg                                                                                   <  2:3433599/151‑1 (MQ=255)

       ggCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTg                                                                                   >  1:4876088/1‑151 (MQ=255)

        gCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGTAGCGAACCCCAGGGTGTATTCCGGTGCGTCGTTCTTCTGCAGGCTGTTGTTGGCCGTGTTGa                                                                                  >  1:3840409/1‑151 (MQ=255)

          aCTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCAGGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACCCAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGc                                                                                <  2:2435381/151‑1 (MQ=255)

          aCTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGc                                                                                >  1:2620284/1‑151 (MQ=255)

             tAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGAt                                                                             >  1:2505896/1‑151 (MQ=255)

              aaTCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATa                                                                            >  1:1035700/1‑151 (MQ=255)

               aTCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaa                                                                           <  1:1225994/151‑2 (MQ=255)

                tctcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaag                                                                          <  2:2329798/151‑3 (MQ=255)

                tctcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaag                                                                          <  1:4361584/151‑3 (MQ=255)

                 ctcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCCTCTGCAGGCTGTTGTAGGCCGGGTTGAGCGATAAGa                                                                         >  1:3227815/1‑151 (MQ=255)

                  tcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGAt                                                                        >  2:264590/1‑151 (MQ=255)

                   cAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATg                                                                       <  2:2222218/151‑1 (MQ=255)

                    aGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGt                                                                      >  2:2831075/1‑151 (MQ=255)

                    aGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGt                                                                      <  1:174657/151‑1 (MQ=255)

                    aGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGt                                                                      <  1:2666376/151‑1 (MQ=255)

                     gCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAGTGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGtt                                                                     <  2:3183831/151‑1 (MQ=255)

                       aTGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGa                                                                   <  2:4285089/151‑1 (MQ=255)

                       aTGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAAGCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGa                                                                   <  1:3309744/151‑1 (MQ=255)

                         gACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAAc                                                                 <  2:2917519/151‑1 (MQ=255)

                           gggCCGTATGCGTTGCCCGCACGCTGACCAATGGCGCTGCCGCTGCGCCAATGGCCCCGAACACAGGTGAAGCGAGCCCCAGGGTGTATTCCGGTGCGTCGCGCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACca                                                               <  2:4267631/150‑1 (MQ=11)

                           cGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACca                                                               <  2:4328213/151‑1 (MQ=255)

                           cGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACca                                                               <  2:2756774/151‑1 (MQ=255)

                            ggCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGCTGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCATGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcac                                                              >  2:4013319/1‑151 (MQ=14)

                            ggCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcac                                                              <  1:4343010/151‑1 (MQ=255)

                            ggCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcac                                                              >  1:423475/1‑151 (MQ=255)

                            ggCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcac                                                              <  1:2978968/151‑1 (MQ=255)

                             gCCTTGTGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcaca                                                             <  2:1394080/151‑1 (MQ=255)

                             gCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcaca                                                             >  1:3253703/1‑151 (MQ=255)

                              ccTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGGCGCTCTTCTGCAGGCTGTTGTAGGCCGGGTTGAGCGATAAGATGTTGAACCACAg                                                            >  1:2068103/1‑151 (MQ=255)

                               cTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGc                                                           >  1:431382/1‑151 (MQ=255)

                               cTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGc                                                           >  1:1017084/1‑151 (MQ=255)

                                  aTGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATc                                                        >  2:2732588/1‑151 (MQ=255)

                                   tGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATcc                                                       <  2:257002/151‑1 (MQ=255)

                                   tGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATcc                                                       >  1:2340172/1‑151 (MQ=255)

                                   tGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATcc                                                       >  2:2425648/1‑151 (MQ=255)

                                    gCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccc                                                      <  1:1947924/151‑1 (MQ=255)

                                    gCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccc                                                      >  1:1610010/1‑151 (MQ=255)

                                      gTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccctg                                                    >  1:2428745/1‑149 (MQ=255)

                                          cccGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGtt                                                <  2:4829486/151‑1 (MQ=255)

                                           ccGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCACGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGttt                                               <  1:1913669/151‑1 (MQ=11)

                                            cGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTa                                              <  2:2082517/151‑1 (MQ=11)

                                            cGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTa                                              >  1:207040/1‑151 (MQ=255)

                                             gCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTaa                                             <  2:3849530/151‑1 (MQ=11)

                                             gCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTaa                                             >  1:135280/1‑151 (MQ=11)

                                             gCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTaa                                             <  2:3263858/151‑1 (MQ=11)

                                             gCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTCCTGCAGGCTGTTGTAGGGCGTGTTGTGCGATAAGATGTTGAACCACTGCATCCCTGCGTTTaa                                             >  2:1502358/1‑151 (MQ=11)

                                              cACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTaaa                                            <  1:654495/151‑1 (MQ=11)

                                              cACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTaaa                                            <  2:3025813/151‑1 (MQ=11)

                                               aCGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAac                                           <  2:1643845/151‑1 (MQ=11)

                                                cGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAacc                                          <  2:4854057/151‑1 (MQ=11)

                                                 gCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAacca                                         <  1:777015/151‑1 (MQ=11)

                                                  cTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccac                                        <  2:419402/151‑1 (MQ=11)

                                                   tGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacc                                       <  2:2458721/151‑1 (MQ=11)

                                                   tGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacc                                       >  1:3344662/1‑151 (MQ=255)

                                                   tGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacc                                       <  1:1785768/151‑1 (MQ=11)

                                                   tGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacc                                       <  2:4544587/151‑1 (MQ=11)

                                                    gACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacca                                      >  1:872784/1‑151 (MQ=11)

                                                    gACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacca                                      >  1:241145/1‑151 (MQ=255)

                                                     aCCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAGGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccac                                     >  1:3933232/1‑151 (MQ=11)

                                                     aCCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccac                                     >  2:3575506/1‑151 (MQ=11)

                                                      ccAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccacc                                    <  1:4004554/151‑1 (MQ=11)

                                                         ctGTCGCTGCCGCTGCGCCAATCCCCCCGAACACAGATGAAGCGAACCGCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccaccacc                                 <  2:3919750/150‑1 (MQ=11)

                                                         aTGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccaccacc                                 <  1:234368/151‑1 (MQ=11)

                                                          tGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCg                                >  2:3488532/1‑151 (MQ=255)

                                                            tCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGcc                              <  1:4702949/151‑1 (MQ=11)

                                                              gctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccgg                            >  2:2323298/1‑149 (MQ=255)

                                                              gctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccgg                            <  2:3706058/151‑3 (MQ=11)

                                                               ctgccgctgcGCCAATCACCCCGATCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                           <  2:4877333/151‑1 (MQ=11)

                                                               ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccgga                           >  2:877526/1‑148 (MQ=11)

                                                               ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                           >  2:4612694/1‑151 (MQ=11)

                                                                  cctctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGt                        >  1:1366444/4‑151 (MQ=11)

                                                                  ccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGt                        <  1:4719877/151‑1 (MQ=11)

                                                                   cgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTg                       <  1:2694689/151‑1 (MQ=11)

                                                                    gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      <  1:363504/151‑1 (MQ=11)

                                                                    gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      >  1:2533311/1‑151 (MQ=11)

                                                                     ctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAg                     >  2:1596546/1‑151 (MQ=11)

                                                                      tgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGc                    >  2:2263460/1‑151 (MQ=11)

                                                                       gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCa                   >  2:1110304/1‑151 (MQ=11)

                                                                        cgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCaa                  >  2:1447897/1‑151 (MQ=11)

                                                                           cAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAccc               <  2:288247/151‑1 (MQ=11)

                                                                              tCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAg            <  1:1564266/151‑1 (MQ=11)

                                                                              tCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAg            >  1:1342714/1‑151 (MQ=11)

                                                                              tCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAg            >  1:1341313/1‑151 (MQ=255)

                                                                                 ccccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGaa         <  1:2936673/151‑1 (MQ=11)

                                                                                   ccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGt       <  2:3688251/151‑1 (MQ=11)

                                                                                    cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGATGTGAGCAACCCAAGGAAGtt      >  2:539838/1‑151 (MQ=11)

                                                                                    cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGtt      <  1:3547360/151‑1 (MQ=11)

                                                                                     gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTg     >  1:5138710/1‑151 (MQ=11)

                                                                                     gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTg     <  2:81181/151‑1 (MQ=11)

                                                                                      aaCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTgg    <  1:1068993/151‑1 (MQ=11)

                                                                                       acacAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTggg   <  1:1169689/151‑1 (MQ=11)

GCGCTGTGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGT  >  CP009974/4543622‑4543860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`