BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,544,682	T→G	S147S (TCA→TCC)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,544,682	0	T	G	99.2%	441.0 / ‑4.2	134	S147S (TCA→TCC)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (66/67); total (66/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 134 total aligned reads displayed.

GCGCAGGGCGGCAATCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCG  >  CP009974/4544550‑4544828

gggcggggCGGGGAGCCGCCGTGCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGCAGACATCGAGGCGCTGTGCCAGGCGCGCGCAATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAgg                                                                                                                                  <  2:3280/146‑1 (MQ=1)

                             cggcgcgtTCGGCGAGGAAGCGCCGGTGTTTGTCGGCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGCGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCt                                                                                                     <  1:526772/148‑1 (MQ=1)

                                                   cgggTAGTTGGGGACATGGAGGGGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAg                                                                               <  2:3786414/149‑1 (MQ=11)

                                                   ccGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAg                                                                               <  2:4019001/151‑1 (MQ=2)

                                                       tATTTGTCGACATCGGGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                           <  2:2015662/151‑1 (MQ=255)

                                                       tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                           >  1:2472589/1‑151 (MQ=16)

                                                         tttGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCa                                                                         >  1:4856718/1‑151 (MQ=19)

                                                         tttGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCa                                                                         >  1:2992530/1‑151 (MQ=19)

                                                         ttggTCGGCGTCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCa                                                                         <  2:2846727/148‑1 (MQ=11)

                                                          ttGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCAt                                                                        <  2:4829577/151‑1 (MQ=19)

                                                            gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                      <  2:2201410/151‑1 (MQ=255)

                                                            gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                      <  1:3712977/151‑1 (MQ=255)

                                                            gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCAATTGGGGGAGTAATCTTCATGGGAGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                      <  1:1099643/151‑1 (MQ=255)

                                                             tCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccg                                                                     >  1:3477674/1‑151 (MQ=19)

                                                             tCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccc                                                                     >  2:2654760/1‑150 (MQ=255)

                                                              cGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGGGCATccgc                                                                    >  2:4036775/1‑151 (MQ=255)

                                                               gACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgcc                                                                   >  2:209719/1‑151 (MQ=18)

                                                                aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                  <  2:3421336/151‑1 (MQ=255)

                                                                 gaTCGGGGCGCTGTGCCAGGCGCGCGTCATTGGGGGAGTGATCTTCGTGGGTGAGGGCCTGGAGATGGGGCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCgg                                                                 <  2:271947/150‑1 (MQ=11)

                                                                  aTCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCggg                                                                >  1:4220841/1‑151 (MQ=255)

                                                                    ggAGGCGCTGTGCCAGGCGCGGGCCATTGGGGGAGTAATCTTCGTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                              <  2:1439750/150‑1 (MQ=255)

                                                                    cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                              <  2:367133/151‑1 (MQ=25)

                                                                     gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                             <  1:4342244/151‑1 (MQ=255)

                                                                     gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGCGAGGGGGCATCCGCCGGGTAc                                                             >  2:2305421/1‑151 (MQ=255)

                                                                      aGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACa                                                            >  1:4510769/1‑151 (MQ=255)

                                                                       ggCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAg                                                           <  1:564887/151‑1 (MQ=255)

                                                                         ggcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGc                                                         <  2:4545271/150‑1 (MQ=255)

                                                                          gcTGTGCCAGGCGGGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                        <  2:698003/151‑1 (MQ=255)

                                                                          gcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                        >  1:3247164/1‑151 (MQ=255)

                                                                           cTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGCACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctc                                                       <  1:3935372/151‑1 (MQ=255)

                                                                           cTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctc                                                       <  1:4416325/151‑1 (MQ=255)

                                                                           cTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctc                                                       <  2:131741/151‑1 (MQ=255)

                                                                            tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctct                                                      >  2:4622052/1‑151 (MQ=255)

                                                                             gtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGGGCATCCGCCGGGTACAGGCtctc                                                     >  2:4199801/1‑151 (MQ=255)

                                                                               gCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCCGCGCGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGCGAGGGGGCATCCGCCGGGTACAGGCCCTccc                                                   >  2:1640839/1‑150 (MQ=11)

                                                                                 cAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGc                                                 <  1:4560751/151‑1 (MQ=255)

                                                                                 cAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGc                                                 <  2:1129128/151‑1 (MQ=255)

                                                                                 cAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGGGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGc                                                 >  1:2811210/1‑151 (MQ=255)

                                                                                  aGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCg                                                <  2:5097470/151‑1 (MQ=255)

                                                                                    gcgcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTc                                              <  1:422342/151‑1 (MQ=255)

                                                                                      gcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCtt                                            >  2:2626014/1‑151 (MQ=255)

                                                                                       cgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGCCTCTCCAGCGTCtcc                                           >  2:1103928/1‑149 (MQ=255)

                                                                                       cgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCACGCCCCCGGCGCGAAGAGCCCAGCACGAACACGGCCAGTTCCGGGGCGGGGGCATCCGCCGGGCAGAGGCCCCCCAGCgccgtg                                           >  1:858197/1‑146 (MQ=11)

                                                                                        gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                          <  1:1100573/151‑1 (MQ=255)

                                                                                        gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                          >  2:4243438/1‑151 (MQ=255)

                                                                                        gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                          <  1:1101362/151‑1 (MQ=255)

                                                                                        gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                          >  1:5013715/1‑151 (MQ=255)

                                                                                        gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                          <  1:2026125/151‑1 (MQ=255)

                                                                                        gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                          >  1:842973/1‑151 (MQ=255)

                                                                                         cgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                         >  1:3400878/1‑151 (MQ=255)

                                                                                          gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                        >  2:2089720/1‑151 (MQ=255)

                                                                                            cctggggggAGTAATCTTCATGGGTGAGGGACTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                      <  2:5169162/147‑1 (MQ=255)

                                                                                            cagtGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                      <  2:3801695/148‑1 (MQ=255)

                                                                                            cATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                      >  1:1294716/1‑151 (MQ=255)

                                                                                              ttGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTCGTTcagcc                                    >  2:3615105/1‑150 (MQ=255)

                                                                                               tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                   <  1:413267/151‑1 (MQ=255)

                                                                                                gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                  <  1:650495/151‑1 (MQ=255)

                                                                                                gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                  >  2:771495/1‑151 (MQ=255)

                                                                                                gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCCCGTTCagcagc                                  >  2:1880343/1‑151 (MQ=255)

                                                                                                 ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                 <  2:3190447/151‑1 (MQ=255)

                                                                                                   ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                               >  2:3429460/1‑151 (MQ=255)

                                                                                                   ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                               <  2:2902820/151‑1 (MQ=255)

                                                                                                    gAGTGATCTTCATGGGTGAGGGCCTGGAAATGGGGCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATgg                              <  2:4143042/151‑1 (MQ=255)

                                                                                                    gAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATgg                              <  1:4564408/151‑1 (MQ=255)

                                                                                                    gAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATgg                              <  2:2976896/151‑1 (MQ=255)

                                                                                                      gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                            >  1:1409609/1‑151 (MQ=255)

                                                                                                      gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                            >  1:4821765/1‑151 (MQ=255)

                                                                                                        aaTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAc                          >  1:2787990/1‑151 (MQ=255)

                                                                                                        aaTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAc                          <  2:1451640/151‑1 (MQ=255)

                                                                                                         aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAcc                         >  1:438442/1‑151 (MQ=255)

                                                                                                         aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAcc                         >  1:1952219/1‑151 (MQ=255)

                                                                                                           cTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAg                       <  1:217185/151‑1 (MQ=255)

                                                                                                           cTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAg                       <  2:4293875/151‑1 (MQ=255)

                                                                                                           cTTCATGGGGGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAg                       <  2:249459/151‑1 (MQ=255)

                                                                                                            ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                      <  2:1164396/151‑1 (MQ=255)

                                                                                                             tCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGt                     >  1:3462554/1‑151 (MQ=255)

                                                                                                             tCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGt                     >  1:4855035/1‑151 (MQ=255)

                                                                                                             tCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGt                     >  1:4855388/1‑151 (MQ=255)

                                                                                                              cATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGtt                    <  2:385247/151‑1 (MQ=255)

                                                                                                                tGGGTGAGGGCTTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGGGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGc                  <  2:566222/151‑1 (MQ=255)

                                                                                                                tGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGc                  >  2:5100996/1‑151 (MQ=255)

                                                                                                                 gggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCg                 >  2:368798/1‑151 (MQ=255)

                                                                                                                 gggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCg                 >  2:1464127/1‑151 (MQ=255)

                                                                                                                 gggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCGGAACGCGGCCAGTTCCGGTGCGGGGGCATCCGCCGGGTACAGGCTCCCCACCGCCGTGCTCAGCAGCATCGCGCCCAGGTTGCg                 >  2:1480925/1‑151 (MQ=11)

                                                                                                                    tGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATg              <  1:1533807/151‑1 (MQ=255)

                                                                                                                     gAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATgg             <  1:3023493/151‑1 (MQ=255)

                                                                                                                     gAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATgg             >  2:3183273/1‑151 (MQ=255)

                                                                                                                      aGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt            >  1:4726463/1‑151 (MQ=255)

                                                                                                                       gggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTg           <  2:857599/151‑1 (MQ=255)

                                                                                                                          gcTGGAAATGGGTCGCGGCGCGCGGCAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCGGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa        <  2:3370205/150‑1 (MQ=11)

                                                                                                                          ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa        <  1:5038014/151‑1 (MQ=255)

                                                                                                                          ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa        <  1:5038196/151‑1 (MQ=255)

                                                                                                                           cTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAg       <  2:568802/151‑1 (MQ=255)

                                                                                                                            tGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGt      >  1:4027555/1‑151 (MQ=255)

                                                                                                                             ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGGACGCGGCCAGTTCCGGTGAGGGGGCATCGGCCGGGTACAGGCTCTCCAGAGTCGTGTTCAGCAGCAGGGCGACCTGGTTGCGGTGGGCAAAggc     >  2:1404786/1‑149 (MQ=11)

                                                                                                                             ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc     >  1:243584/1‑151 (MQ=255)

                                                                                                                             ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc     <  1:2778945/151‑1 (MQ=255)

                                                                                                                               aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc   <  1:195107/151‑1 (MQ=255)

                                                                                                                               aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc   >  2:1673907/1‑151 (MQ=255)

                                                                                                                                aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAACGTcgcg  >  1:4587366/1‑151 (MQ=255)

GCGCAGGGCGGCAATCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCG  >  CP009974/4544550‑4544828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`