BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,544,907	T→C	T72T (ACA→ACG)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,544,907	0	T	C	98.6%	403.7 / NA	144	T72T (ACA→ACG)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (74/68); minor base G (0/2); total (74/70)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

CGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTTGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTGGCGG  >  CP009974/4544760‑4545054

 gccgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTcgtgt                                                                                                                                                 <  1:3918274/151‑1 (MQ=14)

 gccgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTcgtgt                                                                                                                                                 >  1:2706298/1‑151 (MQ=17)

 gccgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTcgtgt                                                                                                                                                 >  1:1266208/1‑151 (MQ=17)

   ggggTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               <  2:5167542/150‑1 (MQ=14)

   cgGGTGCAGCCTCTCCAGCGTCTTGGTCAGCAGCATGGCGACGAGGTTGCGATGGTGAAAGGCGCGGGTCTGCGGGCTGGGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               <  2:5013715/151‑1 (MQ=11)

   cgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               >  1:2472531/1‑151 (MQ=17)

   cgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               >  1:1899540/1‑151 (MQ=17)

   cgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTGGGGCGGGTTTCGTGTGc                                                                                                                                               >  2:68100/1‑151 (MQ=14)

    gggTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCt                                                                                                                                              <  2:4046324/151‑1 (MQ=14)

    gggTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCt                                                                                                                                              <  2:2376883/151‑1 (MQ=14)

     ggTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTc                                                                                                                                             >  1:4376073/1‑151 (MQ=17)

     ggTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTc                                                                                                                                             >  2:1084159/1‑151 (MQ=17)

       tACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCa                                                                                                                                           >  1:1993153/1‑151 (MQ=17)

       tACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGGTTCGTGTGCTCCa                                                                                                                                           >  1:4847180/1‑151 (MQ=17)

          aGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGtt                                                                                                                                        >  2:3295009/1‑151 (MQ=17)

             ctctCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCt                                                                                                                                     >  1:3501932/1‑151 (MQ=14)

             ctctCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCt                                                                                                                                     >  1:3121292/1‑151 (MQ=14)

             ctctCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCt                                                                                                                                     >  1:1126609/1‑151 (MQ=14)

             ctctCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGGTGcg                                                                                                                                     >  2:4803075/1‑150 (MQ=14)

             ctctCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGCTCGGCCTCCAGCCGGTAGCATGCCGGCACGCGGTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGGGGCCCAGTTGcc                                                                                                                                     >  2:4803074/1‑150 (MQ=11)

              tctcCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTc                                                                                                                                    >  1:2867603/1‑151 (MQ=14)

              tctcCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTc                                                                                                                                    <  1:409497/151‑1 (MQ=14)

               ctcCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTcg                                                                                                                                   >  1:4945225/1‑151 (MQ=11)

                                                                                tGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAt                                                                  >  2:3701915/1‑151 (MQ=11)

                                                                                tGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAt                                                                  >  2:4507098/1‑151 (MQ=11)

                                                                                tGAGCTGGTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAt                                                                  <  1:4732434/151‑1 (MQ=11)

                                                                                 gAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATg                                                                 <  1:771495/151‑1 (MQ=14)

                                                                                 gAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATg                                                                 <  1:2074386/151‑1 (MQ=14)

                                                                                 gAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGCTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGTCGAGACCGCCAGGCTCAGGTCCAGGGCCATg                                                                 >  2:3707891/1‑151 (MQ=9)

                                                                                  aGCTGTTCGGCGTCCAGCCGGTAGCGTGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGc                                                                <  2:1650094/151‑1 (MQ=9)

                                                                                   gCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTCGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGcc                                                               >  2:24140/1‑151 (MQ=14)

                                                                                     tGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCtt                                                             >  2:1403778/1‑151 (MQ=14)

                                                                                     tGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCtt                                                             <  2:803486/151‑1 (MQ=17)

                                                                                      gTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtc                                                            <  1:2645825/151‑1 (MQ=17)

                                                                                       ttCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGTGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCTAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcg                                                           >  1:1043528/1‑151 (MQ=9)

                                                                                       ttCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcg                                                           <  2:3362385/151‑1 (MQ=17)

                                                                                       ttCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcg                                                           <  1:140073/151‑1 (MQ=17)

                                                                                        tCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcgg                                                          >  1:1679003/1‑151 (MQ=17)

                                                                                         cGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggc                                                         <  1:269322/151‑1 (MQ=14)

                                                                                         cGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggc                                                         <  1:2494382/151‑1 (MQ=17)

                                                                                          ggCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcg                                                        >  1:288891/1‑151 (MQ=18)

                                                                                           gCATCCAGCCGGTGGCAGGCCGGCACGCGCTCGTGGGCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgt                                                       <  2:3627228/151‑1 (MQ=11)

                                                                                            gatggggCCGGTAGCAGGCCGGCAGGCGCTCCTGCTCGGGAGGGTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtc                                                      <  2:4378535/145‑1 (MQ=11)

                                                                                            cATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtc                                                      >  1:5222253/1‑151 (MQ=21)

                                                                                             aTCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcg                                                     <  1:4584144/151‑1 (MQ=18)

                                                                                                cAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcggcg                                                  >  1:140863/1‑151 (MQ=18)

                                                                                                cAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcggcg                                                  <  1:209719/151‑1 (MQ=25)

                                                                                                 gggcGGTAGCAGGCCGGCACGCGCTGGTGCTCGGGAGGGTGGGCGGGTTTGGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCACGCCTTCGGCGTCGGCGa                                                 <  2:1528117/148‑1 (MQ=11)

                                                                                                 aGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGa                                                 >  1:3637897/1‑151 (MQ=18)

                                                                                                 aGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGa                                                 <  1:5091684/151‑1 (MQ=25)

                                                                                                 aGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCACCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGCCCAGGGCCATGCCTTCGGCGTCGGCGa                                                 >  2:1087789/1‑151 (MQ=16)

                                                                                                  gCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAg                                                <  1:1880343/151‑1 (MQ=25)

                                                                                                    cGGTGGCATGCCGGCACGCGCTCCGGGTCGGGAGGGTGGGCGGGTTTGGTGTGGTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCGGGGAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAGGCCTTCGGCGTCGGCGAGca                                              <  2:3539490/151‑1 (MQ=11)

                                                                                                     ggTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcac                                             >  2:2796941/1‑151 (MQ=37)

                                                                                                     ggTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcac                                             <  2:1188953/151‑1 (MQ=37)

                                                                                                      gTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcacc                                            <  2:3754994/151‑1 (MQ=37)

                                                                                                        ggCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccac                                          <  2:4220841/150‑1 (MQ=255)

                                                                                                        agaaTGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccac                                          <  1:1103928/148‑1 (MQ=255)

                                                                                                          cATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccacca                                        <  1:3429460/151‑1 (MQ=255)

                                                                                                             gCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccacca                                     >  1:745868/1‑151 (MQ=37)

                                                                                                              ccGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTTGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccaccac                                    >  2:2912539/1‑151 (MQ=25)

                                                                                                              ccGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccaccac                                    <  1:5100996/151‑1 (MQ=255)

                                                                                                              ccGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccaccac                                    <  2:533215/151‑1 (MQ=255)

                                                                                                               cGGCACGCGCTCCTGCTCGGGAGGTTGGGCGCGTTTCATGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGCGCCATGCCTTCGGCGTCGGCGAGCaccaccaccacc                                   <  2:4102207/151‑1 (MQ=255)

                                                                                                                   aCGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGcc                               >  2:3842184/1‑150 (MQ=37)

                                                                                                                   aCGCGCTCCTGCTCGGGAGGGTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATTCCTTCGGCGTCGGCGAGCACCACCACCACCGGCa                               >  1:952669/1‑151 (MQ=255)

                                                                                                                    cgcgCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCAc                              <  1:540104/151‑1 (MQ=255)

                                                                                                                     gcgcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACt                             >  1:2838295/1‑151 (MQ=38)

                                                                                                                       gcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACttt                           <  2:1501303/151‑1 (MQ=38)

                                                                                                                        cTCCTGCTGGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          <  2:2063421/151‑1 (MQ=255)

                                                                                                                        cTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACTGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          >  2:4981728/1‑151 (MQ=25)

                                                                                                                        cTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          <  2:745868/151‑1 (MQ=38)

                                                                                                                        cTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          >  1:324315/1‑151 (MQ=38)

                                                                                                                        cTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          <  1:4975941/151‑1 (MQ=255)

                                                                                                                         tCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCg                         <  1:898808/151‑1 (MQ=255)

                                                                                                                         tCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCg                         >  1:2582793/1‑151 (MQ=255)

                                                                                                                             gcccGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTg                     <  2:3388668/148‑1 (MQ=255)

                                                                                                                              cTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCggg                    >  2:4417525/1‑151 (MQ=255)

                                                                                                                              cTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTgg                    >  2:3053343/1‑151 (MQ=255)

                                                                                                                               tCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTggg                   <  1:3049066/151‑1 (MQ=255)

                                                                                                                               tCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTggg                   <  2:842973/151‑1 (MQ=255)

                                                                                                                                  ggAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTcc                >  2:1854990/1‑151 (MQ=255)

                                                                                                                                   gAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCa               >  1:4854756/1‑151 (MQ=255)

                                                                                                                                   gAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCa               <  1:499690/151‑1 (MQ=255)

                                                                                                                                     ggTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAgg             >  1:4685436/1‑151 (MQ=255)

                                                                                                                                      gTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAggg            >  2:801856/1‑151 (MQ=255)

                                                                                                                                       ttGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCCCCACCGGCACTGTCGGCTGGGTCCCgggg           >  2:4021167/1‑151 (MQ=255)

                                                                                                                                         gggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTa         <  2:27425/151‑1 (MQ=255)

                                                                                                                                         gggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCCGGGTCCCGGGGtg         >  2:4480260/1‑150 (MQ=255)

                                                                                                                                          ggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAg        >  1:633106/1‑151 (MQ=255)

                                                                                                                                           gCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGt       <  1:4591953/151‑1 (MQ=255)

                                                                                                                                           gCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGt       >  1:4638959/1‑151 (MQ=255)

                                                                                                                                            ggggTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTg      <  1:1480925/150‑1 (MQ=255)

                                                                                                                                            ggggTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTg      <  1:131680/150‑1 (MQ=255)

                                                                                                                                            cGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTg      <  1:2911247/151‑1 (MQ=255)

                                                                                                                                            cGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTg      <  1:132123/151‑1 (MQ=255)

                                                                                                                                            cGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTg      <  2:2411166/151‑1 (MQ=255)

                                                                                                                                             gggTTTCGTGTGCTCCGGTTGCTCGGCCGGCATCAGGTCACGCAGTCGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTgg     <  2:4586610/151‑1 (MQ=255)

                                                                                                                                               gTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTggcg   <  1:4622052/151‑1 (MQ=255)

                                                                                                                                                tttcGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCCCAGGCCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCTGCTTGGTCCTGGGGTAGTggcgg  >  2:1249259/1‑151 (MQ=14)

CGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTTGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTGGCGG  >  CP009974/4544760‑4545054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`