Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,728,247 G→A G159D (GGC→GAC)  RPPX_21060 → quinohemoprotein amine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,728,2470GA95.8% 825.5 / 13.7 240G159D (GGC→GAC) RPPX_21060quinohemoprotein amine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base A (114/114);  total (122/118)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

Only 100 of 243 total aligned reads displayed.

GTAGCCGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGGCCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTACACGGTGGCCCTGCCGCT  >  CP009974/4728099‑4728394
                                                                                                                                                    |                                                                                                                                                   
gTAGCCGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGAcc                                                                                                                                                   >  1:460785/1‑151 (MQ=255)
  aGCCGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCtt                                                                                                                                                 <  1:1579855/151‑1 (MQ=255)
   gCCGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTc                                                                                                                                                <  1:4660137/151‑1 (MQ=255)
   gCCGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTc                                                                                                                                                <  1:1846921/151‑1 (MQ=255)
    ccGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCa                                                                                                                                               <  2:2793136/151‑1 (MQ=255)
      ggTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAgg                                                                                                                                             >  1:5145677/1‑151 (MQ=255)
      ggTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAgg                                                                                                                                             >  2:646756/1‑151 (MQ=255)
          aGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCaa                                                                                                                                         <  2:3911171/151‑1 (MQ=255)
          aGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCaa                                                                                                                                         >  2:268270/1‑151 (MQ=255)
            gTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGc                                                                                                                                       >  1:60882/1‑151 (MQ=255)
             tCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGcc                                                                                                                                      <  1:3054004/151‑1 (MQ=255)
             gcGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGcc                                                                                                                                      <  2:3988171/150‑1 (MQ=255)
              cGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGccc                                                                                                                                     >  2:5198064/1‑151 (MQ=255)
                 ccGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCcgta                                                                                                                                  >  1:2457499/1‑151 (MQ=255)
                  cGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCcgtac                                                                                                                                 >  2:1991716/1‑151 (MQ=255)
                  cGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCcgtac                                                                                                                                 <  1:3619916/151‑1 (MQ=255)
                      cGATCTATTCGTTCGCCATCCGCCCTGACGGCAAAGAGGTTTACGCCACGGTCAACCCGACCTAGAGGCCGAACGACCATTACGTGGTCAAACCACCGCGGCTGGCGGGGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCgtacgtac                                                                                                                             >  1:667313/1‑151 (MQ=255)
                      cGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCgtacgtac                                                                                                                             >  1:3181567/1‑151 (MQ=255)
                      cGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCgtacgtac                                                                                                                             <  1:1758279/151‑1 (MQ=255)
                         tCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCtt                                                                                                                          <  1:648090/151‑1 (MQ=255)
                         tCTATGCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATGACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCtt                                                                                                                          <  2:3940429/151‑1 (MQ=255)
                          cTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGGCCTTCAGGCCAAGCCCGTACGTACCTTc                                                                                                                         >  2:797648/1‑151 (MQ=255)
                          cTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTc                                                                                                                         <  1:1589242/151‑1 (MQ=255)
                           tATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTcc                                                                                                                        >  1:738426/1‑151 (MQ=255)
                           tATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTcc                                                                                                                        >  1:832590/1‑151 (MQ=255)
                           tATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTcc                                                                                                                        <  2:1273583/151‑1 (MQ=255)
                             ttcgttcgCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCg                                                                                                                      >  2:4762547/1‑151 (MQ=255)
                                gttcgCCATCAGTCCTGTCGGCATAGAGGTGTACGCCACTGCCATCCCGACCCAGTGGCCGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGCGTTCAGCACCGCCGATGACCCTCAGGCCAAGCCCGCCCGTCCCCTCCCCATg                                                                                                                   >  2:184645/1‑151 (MQ=255)
                                  tcgCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCACACCACCGCGGCTGGAGGCGTCCAGCCCCGCTGATGCCCTTCAGGCCACGCCCGTACCTACCTTCCCGATGcc                                                                                                                 >  2:4955440/1‑151 (MQ=255)
                                  tcgCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGcc                                                                                                                 <  2:1602947/151‑1 (MQ=255)
                                       aTCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGcc                                                                                                            >  2:451568/1‑151 (MQ=255)
                                         cAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAg                                                                                                          >  2:74529/1‑151 (MQ=255)
                                          aGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAgg                                                                                                         >  2:719417/1‑151 (MQ=255)
                                          aGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAgg                                                                                                         <  2:4244975/151‑1 (MQ=255)
                                            cccTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGgtg                                                                                                       <  2:412031/151‑1 (MQ=255)
                                              cTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTa                                                                                                     >  1:1961263/1‑151 (MQ=255)
                                               tGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTAc                                                                                                    >  2:936740/1‑151 (MQ=255)
                                                gACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTAcc                                                                                                   >  2:4441453/1‑151 (MQ=255)
                                                  cGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTg                                                                                                 >  1:1459043/1‑151 (MQ=255)
                                                   ggCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGa                                                                                                <  1:2296983/151‑1 (MQ=255)
                                                     cAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATg                                                                                              >  1:3370675/1‑151 (MQ=255)
                                                      aaaGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATgc                                                                                             >  2:2432122/1‑151 (MQ=255)
                                                      aaaGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATgc                                                                                             >  2:132124/1‑151 (MQ=255)
                                                      aaaGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATgc                                                                                             >  1:4879958/1‑151 (MQ=255)
                                                           ggTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGcc                                                                                        <  1:1413221/151‑1 (MQ=255)
                                                            gtgtACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCt                                                                                       <  2:4640026/151‑1 (MQ=255)
                                                             tgtACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTc                                                                                      >  1:3296825/1‑151 (MQ=255)
                                                                aCGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGGCCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCcga                                                                                   <  2:3371266/151‑1 (MQ=255)
                                                                  gCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCcgacg                                                                                 >  1:402216/1‑151 (MQ=255)
                                                                   ccACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCcgacga                                                                                >  1:2599235/1‑151 (MQ=255)
                                                                    cACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGAt                                                                               <  1:1966799/151‑1 (MQ=255)
                                                                    cACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGAt                                                                               >  2:2974091/1‑151 (MQ=255)
                                                                      cGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCATCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATgg                                                                             >  2:4590104/1‑151 (MQ=255)
                                                                        gTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCa                                                                           <  1:705320/151‑1 (MQ=255)
                                                                         tCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAg                                                                          <  2:3014661/151‑1 (MQ=255)
                                                                         tCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAg                                                                          >  2:1340974/1‑151 (MQ=255)
                                                                          cAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGc                                                                         >  2:1848870/1‑151 (MQ=255)
                                                                            aCCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCt                                                                       <  2:3771968/151‑1 (MQ=255)
                                                                              ccGACCCAGAGGCTTAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTGCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGt                                                                     <  2:3545433/151‑1 (MQ=255)
                                                                              ccGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGt                                                                     >  1:915253/1‑151 (MQ=255)
                                                                              ccGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGt                                                                     <  1:38010/151‑1 (MQ=255)
                                                                              ccGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGCCAGCCTGt                                                                     >  2:1455406/1‑151 (MQ=255)
                                                                                gACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCcg                                                                                    <  2:3759331/134‑1 (MQ=255)
                                                                                   ccAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGtt                                                                >  1:3926526/1‑151 (MQ=255)
                                                                                     agagGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTgc                                                              >  1:4753629/1‑151 (MQ=255)
                                                                                      gagGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTgcc                                                             >  1:3556825/1‑151 (MQ=255)
                                                                                        ggCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTgccgg                                                           >  2:882104/1‑151 (MQ=255)
                                                                                        ggCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTgccgg                                                           <  2:1961932/151‑1 (MQ=255)
                                                                                          cTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTgccgg                                                           >  2:1494592/1‑149 (MQ=255)
                                                                                            gAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTTCGACGATGGCAGCCTGTACGTTgcttggccg                                                       >  2:2065924/1‑151 (MQ=255)
                                                                                            gAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTgccgggccg                                                       <  2:1752439/151‑1 (MQ=255)
                                                                                             aaCGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTgccgggccgg                                                      >  2:4409334/1‑151 (MQ=255)
                                                                                              aCGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGa                                                     <  1:1249335/151‑1 (MQ=255)
                                                                                              aCGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGa                                                     <  1:3721124/151‑1 (MQ=255)
                                                                                                gACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCGGCCTGTACGTTGCCGGGCCGGACa                                                   >  1:4433092/1‑151 (MQ=255)
                                                                                                gACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACa                                                   >  1:4095487/1‑151 (MQ=255)
                                                                                                gACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACa                                                   <  2:3091966/151‑1 (MQ=255)
                                                                                                  ccATTACGTGGTCCAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACAtt                                                 <  1:4255883/151‑1 (MQ=255)
                                                                                                        cGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAg                                           <  1:4185468/151‑1 (MQ=255)
                                                                                                          tGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGAt                                         <  2:227198/151‑1 (MQ=255)
                                                                                                           ggTCAAACGACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATg                                        <  1:3640837/151‑1 (MQ=255)
                                                                                                            gTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATgg                                       >  1:1849165/1‑151 (MQ=255)
                                                                                                              cAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGGCAGGCGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTCCGTTGCCGGGCCGGACCTTTAGCAGATGGAc                                     >  2:4100151/1‑151 (MQ=255)
                                                                                                                aaCCACGGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGt                                   <  2:3573462/151‑1 (MQ=255)
                                                                                                                 accaccGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTg                                  <  1:3199313/151‑1 (MQ=255)
                                                                                                                 accaccGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTg                                  <  2:2149259/151‑1 (MQ=255)
                                                                                                                  ccaccGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGa                                 >  2:3465916/1‑151 (MQ=255)
                                                                                                                   caccGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGaa                                >  2:2819166/1‑151 (MQ=255)
                                                                                                                    accGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAg                               >  1:2026082/1‑151 (MQ=255)
                                                                                                                    accGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGAGGGACGTGAAg                               >  2:1858707/1‑151 (MQ=255)
                                                                                                                     ccGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGa                              >  1:1011535/1‑151 (MQ=255)
                                                                                                                     ccGCGGCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGa                              <  2:2599235/151‑1 (MQ=255)
                                                                                                                          gCTGGAGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGc                         <  2:1821962/151‑1 (MQ=255)
                                                                                                                               aGGTGTTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTa                    <  1:1311852/151‑1 (MQ=255)
                                                                                                                                  tgtTCAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTacac                 <  1:5198064/151‑1 (MQ=255)
                                                                                                                                  tgtTCAGCACCGCTCATGGCCTTCATGCCAAGCCCGTACGTACCTTCCTGATTCCACGCCAGGTGTAACTGAAGCGCGACACCCACCATGGAAGCATGTTAGTTCGCTGAGCGAACATTAACAATATGAACAACAGGACCAGCAAATacac                 >  2:5011884/1‑151 (MQ=255)
                                                                                                                                      cAGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTACACGGTg             <  1:30896/151‑1 (MQ=255)
                                                                                                                                       aGCACCGCTGATGACCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTACACGGTgg            >  1:1677967/1‑151 (MQ=255)
                                                                                                                                         cACCGCTGATGACCTGCAGGCCAAGCCCGTACGTACCTTCCCGATTCCGCGCCAGGTGTACCTGATGAGCGCCTCCGACGATGGCAGCCTGTACGGTTCCGGGCCGGACAGTTACAAGATGGACGTGAAGAGCGGCAAGTACACGGTGTcc          >  1:2091682/1‑151 (MQ=255)
                                                                                                                                               tgatgaCCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGTGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTACACGGTGGCCCTGCCg    >  1:1803987/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTAGCCGGTGAGGTCGGCCGGTCGATCTATTCGTTCGCCATCAGCCCTGACGGCAAAGAGGTGTACGCCACGGTCAACCCGACCCAGAGGCTGAACGACCATTACGTGGTCAAACCACCGCGGCTGGAGGTGTTCAGCACCGCTGATGGCCTTCAGGCCAAGCCCGTACGTACCTTCCCGATGCCGCGCCAGGTGTACCTGATGCGCGCCTCCGACGATGGCAGCCTGTACGTTGCCGGGCCGGACATTTACAAGATGGACGTGAAGACCGGCAAGTACACGGTGGCCCTGCCGCT  >  CP009974/4728099‑4728394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑