Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,916,154 A→G F315L (TTC→CTC)  RPPX_21810 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,916,1540AG99.6% 926.2 / ‑4.8 258F315L (TTC→CTC) RPPX_21810hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (123/134);  total (123/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 258 total aligned reads displayed.

TGAGGTTCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAATCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTGCGCG  >  CP009974/4916006‑4916301
                                                                                                                                                    |                                                                                                                                                   
tGAGGTTCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTc                                                                                                                                                   >  2:5232178/1‑151 (MQ=255)
 gAGGTTCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCg                                                                                                                                                  <  1:3853841/151‑1 (MQ=255)
    gTTCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGAt                                                                                                                                               >  1:3654487/1‑151 (MQ=255)
    gTTCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGAt                                                                                                                                               <  1:4141097/151‑1 (MQ=255)
     ttCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATg                                                                                                                                              >  1:1656100/1‑151 (MQ=255)
           aagcaaATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGAtc                                                                                                                                        <  2:3309433/151‑1 (MQ=255)
            agcaaATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGAtct                                                                                                                                       <  1:3177013/151‑1 (MQ=255)
               aaaTTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCg                                                                                                                                    <  1:2444665/151‑1 (MQ=255)
                  ttCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCAGGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCtt                                                                                                                                 <  1:485082/151‑1 (MQ=255)
                     gCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATCGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCa                                                                                                                              >  1:2349422/1‑151 (MQ=255)
                      ccTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCAc                                                                                                                             >  2:3430711/1‑151 (MQ=255)
                         gCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTg                                                                                                                          <  1:3672083/151‑1 (MQ=255)
                          cTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTgg                                                                                                                         >  1:996737/1‑151 (MQ=255)
                          cTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTgg                                                                                                                         <  1:3438897/151‑1 (MQ=255)
                             aaCGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGgct                                                                                                                      >  1:4877402/1‑151 (MQ=255)
                             aaCGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGgct                                                                                                                      >  2:2377174/1‑151 (MQ=255)
                               cGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGgctgc                                                                                                                    >  1:1720063/1‑151 (MQ=255)
                               cGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGgctgc                                                                                                                    <  1:2605039/151‑1 (MQ=255)
                                ggCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGgctgct                                                                                                                   >  2:1221438/1‑151 (MQ=255)
                                ggCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGgctgct                                                                                                                   >  1:1480240/1‑151 (MQ=255)
                                  ccAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTg                                                                                                                 >  2:2104855/1‑151 (MQ=255)
                                    aaCTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGa                                                                                                               >  1:2473858/1‑151 (MQ=255)
                                    aaCTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGCGCTGCTTGGa                                                                                                               >  2:3935314/1‑151 (MQ=255)
                                     aCTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGaa                                                                                                              >  1:757496/1‑151 (MQ=255)
                                       tCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACt                                                                                                            >  2:674532/1‑151 (MQ=255)
                                       ccGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACt                                                                                                            <  2:2230043/150‑1 (MQ=255)
                                          tAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTgg                                                                                                         >  1:3533106/1‑151 (MQ=255)
                                          tAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTgg                                                                                                         <  2:3103844/151‑1 (MQ=255)
                                          tAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTgg                                                                                                         >  2:3714917/1‑151 (MQ=255)
                                           aaTGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTggg                                                                                                        >  2:187116/1‑151 (MQ=255)
                                           aaTGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTggg                                                                                                        >  1:1851252/1‑151 (MQ=255)
                                            aTGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTgggg                                                                                                       <  2:786273/151‑1 (MQ=255)
                                             tGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGa                                                                                                      <  1:4227410/151‑1 (MQ=255)
                                                   tGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAgcggcg                                                                                                <  1:18061/151‑1 (MQ=255)
                                                     cACGGCATGCAGGCGGGGGCGTATGGCAATGATGGCTGGATCTTCTCCTTCCAGTGGATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGAACTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGtt                                                                                              <  2:227921/151‑1 (MQ=255)
                                                     cACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGtt                                                                                              <  1:1356461/151‑1 (MQ=255)
                                                     cACGGCATGCAGCCGGGAGCGAAGGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGtt                                                                                              <  2:2720108/151‑1 (MQ=255)
                                                      aCGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTa                                                                                             <  1:3822446/151‑1 (MQ=255)
                                                       cGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTAc                                                                                            <  2:1650385/151‑1 (MQ=255)
                                                         gCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCt                                                                                          >  2:4388954/1‑151 (MQ=255)
                                                         gCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCt                                                                                          <  2:4739823/151‑1 (MQ=255)
                                                          gaagCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAGTCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTc                                                                                         <  2:2515712/148‑1 (MQ=255)
                                                          cATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTc                                                                                         <  2:2599410/151‑1 (MQ=255)
                                                          cATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTc                                                                                         >  2:4409354/1‑151 (MQ=255)
                                                           aTGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCg                                                                                        >  2:484788/1‑151 (MQ=255)
                                                             gCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTa                                                                                      >  1:646370/1‑151 (MQ=255)
                                                              cAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTAc                                                                                     >  1:1040931/1‑151 (MQ=255)
                                                              cAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTAc                                                                                     >  2:3614549/1‑151 (MQ=255)
                                                               gggcGGGAGCGGGTGGGAGTGATGGCTGGGTCTTCACCGTCGGGTAAGTCGCGTAGCGCGGCAAGGGGGTCGGTACCGTTCTCGAGGCGGATGTGGATCTGCGGTTTCTCCTGGGCTGCGTGGAACTTGGGGAGCGGCGTTACCTCGTAcg                                                                                    <  2:4235808/148‑1 (MQ=255)
                                                                gCCGGGAGCGCATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTAcgc                                                                                   <  2:557720/151‑1 (MQ=255)
                                                                  ggggAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGt                                                                                 <  2:2784194/150‑1 (MQ=255)
                                                                  cGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGt                                                                                 >  1:3789977/1‑151 (MQ=255)
                                                                    ggAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTc                                                                               <  1:2025340/151‑1 (MQ=255)
                                                                     gAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCt                                                                              <  1:3942497/151‑1 (MQ=255)
                                                                       gCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTgg                                                                            <  1:952712/151‑1 (MQ=255)
                                                                        cGAATGGCAATGATGGCTGGATCTTCAACTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGt                                                                           <  2:3672453/151‑1 (MQ=255)
                                                                         gAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTa                                                                          >  2:4060053/1‑151 (MQ=255)
                                                                          aaTGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAg                                                                         >  2:1561738/1‑151 (MQ=255)
                                                                             ggCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGaa                                                                      <  2:114744/151‑1 (MQ=255)
                                                                             ggCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGaa                                                                      >  1:1943717/1‑151 (MQ=255)
                                                                                 atgatgGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTGTCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGGACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAgaga                                                                  >  2:5054347/1‑151 (MQ=255)
                                                                                 atgatgGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAgaga                                                                  >  1:4077565/1‑151 (MQ=255)
                                                                                   gatgGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACt                                                                <  1:2228399/151‑1 (MQ=255)
                                                                                      ggCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGcc                                                             >  2:1996127/1‑151 (MQ=255)
                                                                                      ggCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGcc                                                             <  1:4765709/151‑1 (MQ=255)
                                                                                           gATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCttt                                                        <  1:3022394/151‑1 (MQ=255)
                                                                                           gATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCttt                                                        <  2:3789977/151‑1 (MQ=255)
                                                                                                tCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTTCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGATGAGACTGTCGCTTTGTTAt                                                   >  1:450385/1‑151 (MQ=255)
                                                                                                tCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTCCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTAt                                                   <  2:4582509/151‑1 (MQ=255)
                                                                                                         aGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGttt                                          <  2:1191045/151‑1 (MQ=255)
                                                                                                         aGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGttt                                          <  2:4153009/151‑1 (MQ=255)
                                                                                                            aaaTCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTAtct                                                 >  2:2915572/1‑141 (MQ=255)
                                                                                                            aaaTCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTAtct                                                 <  1:2915572/141‑1 (MQ=255)
                                                                                                                cATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTg                                   <  2:4501635/151‑1 (MQ=255)
                                                                                                                cATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTg                                   >  1:309499/1‑151 (MQ=255)
                                                                                                                cATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTg                                   >  1:2533781/1‑151 (MQ=255)
                                                                                                                cATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCGTTGTTATCTCCCGTTTGAACCTg                                   >  2:4983208/1‑151 (MQ=255)
                                                                                                                   gTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCa                                >  1:2133046/1‑151 (MQ=255)
                                                                                                                      gCTCTGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGtt                             <  2:1725090/151‑1 (MQ=255)
                                                                                                                       ctctGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTg                            >  2:1589949/1‑151 (MQ=255)
                                                                                                                          tGCAAGTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCa                         >  2:3071664/1‑151 (MQ=255)
                                                                                                                               gTGAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAg                    <  2:3593503/151‑1 (MQ=255)
                                                                                                                                tGAGACGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTAGGCTGCAAGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAgg                   >  2:4919806/1‑151 (MQ=255)
                                                                                                                                 gAGTCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGgc                  <  1:1259319/151‑1 (MQ=255)
                                                                                                                                    tCGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCGTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGcc               <  1:4718223/151‑1 (MQ=255)
                                                                                                                                     cGCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCa              >  1:2966771/1‑151 (MQ=255)
                                                                                                                                      gCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCaa             >  1:4163068/1‑151 (MQ=255)
                                                                                                                                      gCTATCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCaa             <  1:4965157/151‑1 (MQ=255)
                                                                                                                                         aTCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAatat          >  2:833206/1‑151 (MQ=255)
                                                                                                                                          tCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATAtt         <  2:2482705/151‑1 (MQ=255)
                                                                                                                                          tCGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCAAGTAGGCGCCCATAtt         >  1:4818720/1‑151 (MQ=255)
                                                                                                                                          tCGTGCTCGAGTCGGGTGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATAtt         <  2:5227591/151‑1 (MQ=255)
                                                                                                                                           cGTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTc        >  2:3257022/1‑151 (MQ=255)
                                                                                                                                            gTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCt       <  1:3614549/151‑1 (MQ=255)
                                                                                                                                            gTTCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCt       >  1:3191059/1‑151 (MQ=255)
                                                                                                                                             ttCTCGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTg      <  2:315547/151‑1 (MQ=255)
                                                                                                                                              tctcGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTgc     <  2:3179082/151‑1 (MQ=255)
                                                                                                                                              tctcGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTgc     >  1:2759331/1‑151 (MQ=255)
                                                                                                                                               ctcGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTgcg    >  2:1993388/1‑151 (MQ=255)
                                                                                                                                                 cGAGTCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTgcgcg  <  1:1103790/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGAGGTTCCGCAAGCAAATTCGCCTGCTCAACGGCCAACTCGTAATGCCCTTGCACGGCATGCAGCCGGGAGCGAATGGCAATGATGGCTGGATCTTCACCTTCCAGTAAATCATGTAGCTCTGCAAGTGAGTCGCTATCGTTCTCGAATCGGATGCGGATCTCCGCTTCCACCTGGGCTGCTTGGAACTTGGGGAGCGGCGTTACCTCGTACGCGTTCTGGTAGGAAGAGACTGCCGCTTTGTTATCTCCCGTTTGAACCTGCCAGTTGCCACGTAGGCGCCAATATTCTGCGCG  >  CP009974/4916006‑4916301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑