Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,323 T→A F82Y (TTC→TAC)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,3230TA99.1% 753.8 / NA 211F82Y (TTC→TAC) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (110/99);  minor base C (0/2);  total (110/101)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

GGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGG  >  CP009974/5007176‑5007470
                                                                                                                                                   |                                                                                                                                                   
  aGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCa                                                                                                                                                <  2:1217225/151‑1 (MQ=255)
   gCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCAt                                                                                                                                               <  2:1348324/151‑1 (MQ=255)
   gCCTGAAGCACGCCTACGACAATGTGCCGTTATACCGCCAGCGCTTTGCCGAGTACGGCCCCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGCTCCCCTTCACCGGCACGAACGACCTGCGCGACCCCTACCCCTACGGCAt                                                                                                                                               >  1:2883091/1‑151 (MQ=255)
     cTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGt                                                                                                                                             >  2:4047492/1‑151 (MQ=255)
      tGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGtt                                                                                                                                            <  2:3986994/151‑1 (MQ=255)
         aGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGc                                                                                                                                         <  2:2792847/151‑1 (MQ=255)
           cACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCg                                                                                                                                       >  2:593996/1‑151 (MQ=255)
           cACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCg                                                                                                                                       >  1:2346408/1‑151 (MQ=255)
              gCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGc                                                                                                                                    <  2:2087054/151‑1 (MQ=255)
              gCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGc                                                                                                                                    <  1:3237149/151‑1 (MQ=255)
               ccTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGcc                                                                                                                                   <  2:4466658/151‑1 (MQ=255)
                cTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCACGTTTGCCGTGccc                                                                                                                                  >  2:4237118/1‑151 (MQ=255)
                  aCGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCTCCa                                                                                                                                >  1:183587/1‑151 (MQ=255)
                     aCAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGga                                                                                                                             >  2:4131009/1‑151 (MQ=255)
                      cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                                                            >  1:3891480/1‑151 (MQ=255)
                             ccGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCCTGTTTGCCGTGCCCCCGGAAGAAgtgg                                                                                                                     >  1:4220879/1‑151 (MQ=255)
                             ccGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtgg                                                                                                                     >  2:17857/1‑151 (MQ=255)
                             ccGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtgg                                                                                                                     >  2:3638579/1‑151 (MQ=255)
                              cGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggt                                                                                                                    <  2:3688599/151‑1 (MQ=255)
                               gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                                                                   <  2:1470107/151‑1 (MQ=255)
                               gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                                                                   <  1:3005275/151‑1 (MQ=255)
                                ttGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgc                                                                                                                  >  1:1024025/1‑151 (MQ=255)
                                ttGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgc                                                                                                                  <  1:187150/151‑1 (MQ=255)
                                ttGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgc                                                                                                                  <  2:4789066/151‑1 (MQ=255)
                                 tGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgcg                                                                                                                 >  1:1485800/1‑151 (MQ=255)
                                  gTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgcgc                                                                                                                >  2:4210186/1‑151 (MQ=255)
                                   tACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGcc                                                                                                               >  1:3421221/1‑151 (MQ=255)
                                    aCCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGCGGTGCGCCt                                                                                                              >  2:4344994/1‑151 (MQ=255)
                                      cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                                                            >  1:1753328/1‑151 (MQ=255)
                                      cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                                                            >  2:239897/1‑151 (MQ=255)
                                      cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                                                            >  2:239585/1‑151 (MQ=255)
                                        ccAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAt                                                                                                          >  1:1803786/1‑151 (MQ=255)
                                           gcgcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGcc                                                                                                       >  1:5147481/1‑151 (MQ=255)
                                             gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTc                                                                                                     <  2:3738217/151‑1 (MQ=255)
                                               tttGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCa                                                                                                   <  2:3893504/151‑1 (MQ=255)
                                                 tGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAgt                                                                                                 >  2:818837/1‑150 (MQ=255)
                                                  gCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAgtg                                                                                                <  2:3404794/151‑3 (MQ=255)
                                                    cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                                                              >  2:2070182/1‑151 (MQ=255)
                                                     gAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGca                                                                                             >  1:3112732/1‑151 (MQ=255)
                                                       gTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcacc                                                                                           >  1:3165240/1‑151 (MQ=255)
                                                         aCGGCACCCACCCTGATGGCCTCGAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcaccac                                                                                         <  2:3167158/151‑1 (MQ=255)
                                                         aCGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGCTTGCCGTGCCCCAGGAAGCAGTGGTGCGCCTGCATGCCTCCAGCGGccccac                                                                                         >  2:3022709/1‑151 (MQ=255)
                                                           ggCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCg                                                                                       >  2:1390841/1‑151 (MQ=255)
                                                              acccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCa                                                                                    <  2:1705153/151‑1 (MQ=255)
                                                                   cccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                                                               >  1:476982/1‑151 (MQ=255)
                                                                    ccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGa                                                                              >  1:4182271/1‑151 (MQ=255)
                                                                     cTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGACCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGAc                                                                             <  2:1976134/151‑1 (MQ=255)
                                                                      tgatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                                                            <  2:4368758/151‑1 (MQ=255)
                                                                       gatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATg                                                                                                         <  1:2716232/121‑1 (MQ=255)
                                                                       gatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATg                                                                                                         >  2:2716232/1‑121 (MQ=255)
                                                                        atgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggt                                                                          <  1:464332/151‑1 (MQ=255)
                                                                           aCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggc                                                                       >  2:618904/1‑150 (MQ=255)
                                                                            ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                                                                      >  2:2141790/1‑151 (MQ=255)
                                                                               cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                                                                   >  2:3713488/1‑151 (MQ=255)
                                                                               cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                                                                   >  1:1667531/1‑151 (MQ=255)
                                                                                 aGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTa                                                                 >  1:3516687/1‑151 (MQ=255)
                                                                                     ccTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                                                             <  1:3197128/151‑1 (MQ=255)
                                                                                       tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                                                           >  1:597688/1‑151 (MQ=255)
                                                                                       tGGAAGACCTGGCGAAGTGCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                                                           <  2:1190935/151‑1 (MQ=255)
                                                                                        ggAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAg                                                          <  2:2274468/151‑1 (MQ=255)
                                                                                          aaGACCTGGCGACGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGaa                                                        <  1:841239/151‑1 (MQ=255)
                                                                                          aaGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGac                                                        <  1:2552787/151‑2 (MQ=255)
                                                                                          aaGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGaa                                                        >  2:1940169/1‑151 (MQ=255)
                                                                                           aGACCTGGCGAAGTTCCCCTTCACCGGCAAGCACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAAc                                                       <  2:1667538/151‑1 (MQ=255)
                                                                                           aGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGCGGTCGGCTACCCCCCGAAc                                                       >  2:2849620/1‑151 (MQ=255)
                                                                                              ccTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGat                                                    <  1:2648519/151‑1 (MQ=255)
                                                                                                  gCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCg                                                >  1:1979650/1‑151 (MQ=255)
                                                                                                    gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGac                                              >  1:579366/1‑151 (MQ=255)
                                                                                                      aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGacac                                            >  2:726512/1‑151 (MQ=255)
                                                                                                      aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGacac                                            >  1:2808099/1‑151 (MQ=255)
                                                                                                        ttccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCt                                          <  1:2316049/149‑1 (MQ=255)
                                                                                                         tccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTg                                         <  1:3955190/150‑1 (MQ=255)
                                                                                                         tccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTg                                         <  2:4292487/150‑1 (MQ=255)
                                                                                                           cccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTggg                                       <  1:3710512/151‑1 (MQ=255)
                                                                                                              ttCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCa                                    >  1:2460144/1‑151 (MQ=255)
                                                                                                               tCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCaa                                   >  1:3615356/1‑151 (MQ=255)
                                                                                                               tCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCaa                                   <  1:2146342/151‑1 (MQ=255)
                                                                                                                    ggCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTgg                              <  1:5009028/151‑1 (MQ=255)
                                                                                                                      cAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGtt                            <  2:3125779/151‑1 (MQ=255)
                                                                                                                      cAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGtt                            <  1:4872965/151‑1 (MQ=255)
                                                                                                                       aaGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTg                           >  2:896469/1‑151 (MQ=255)
                                                                                                                       aaGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTg                           >  1:4702338/1‑151 (MQ=255)
                                                                                                                         gAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTTGGCCAATGGGGTTGcc                         >  2:3873033/1‑151 (MQ=255)
                                                                                                                           aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCGTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                       <  2:80042/151‑1 (MQ=255)
                                                                                                                             gaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGtt                     >  1:5094846/4‑151 (MQ=255)
                                                                                                                             gaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGtt                     >  2:557807/4‑151 (MQ=255)
                                                                                                                               ccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTcc                   >  1:4531956/2‑151 (MQ=255)
                                                                                                                               ccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTcc                   <  2:4629652/150‑1 (MQ=255)
                                                                                                                                     cgACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTACGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGt             <  2:2642352/151‑1 (MQ=255)
                                                                                                                                      gACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTg            <  2:2352976/151‑1 (MQ=255)
                                                                                                                                       aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCgcgc           >  2:4736158/1‑151 (MQ=255)
                                                                                                                                       aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGc           <  2:1395226/151‑1 (MQ=255)
                                                                                                                                        cAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcg          >  1:206338/1‑151 (MQ=255)
                                                                                                                                         aaCTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCCGACCGATATCGACCCCTGGGCCCATGTGGTTGCCCGCTCCATCCGTGcgg         >  2:5040934/1‑151 (MQ=255)
                                                                                                                                          aCTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggc        <  1:694466/151‑1 (MQ=255)
                                                                                                                                            tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCCCTGTGGTTGCCCGGTCCATTCGTGcggccg      >  2:3068391/1‑151 (MQ=255)
                                                                                                                                             aCCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccgg     >  2:1024638/1‑151 (MQ=255)
                                                                                                                                             aCCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccgg     <  1:3822359/151‑1 (MQ=255)
                                                                                                                                              ccccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGGGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccggc    >  2:2483519/1‑151 (MQ=255)
                                                                                                                                               cccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCcggcg   <  1:2565323/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGG  >  CP009974/5007176‑5007470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑