Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,375 C→T S99S (AGC→AGT)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,3750CT99.5% 772.5 / NA 207S99S (AGC→AGT) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (97/109);  total (98/109)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACCGG  >  CP009974/5007228‑5007521
                                                                                                                                                   |                                                                                                                                                  
cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                                                                                                                 >  2:2070182/1‑151 (MQ=255)
 gAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcc                                                                                                                                                >  2:2118592/1‑150 (MQ=255)
 gAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGca                                                                                                                                                >  1:3112732/1‑151 (MQ=255)
   gTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcacc                                                                                                                                              >  1:2679093/1‑151 (MQ=255)
       ggCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCg                                                                                                                                          <  1:3514101/151‑1 (MQ=255)
        gCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCgg                                                                                                                                         <  1:1294783/151‑1 (MQ=255)
         cacccaccCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGCCCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAACAACTGCCGCGCCTGCATGCCTCCCGTCGCACCCCCGGc                                                                                                                                        >  2:2865275/1‑151 (MQ=255)
                 cTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGACCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGAc                                                                                                                                <  2:1976134/151‑1 (MQ=255)
                  tgatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                                                                                                               <  2:4368622/151‑1 (MQ=255)
                     tgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtg                                                                                                                            <  2:2670191/151‑1 (MQ=255)
                     tgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtg                                                                                                                            <  1:860280/151‑1 (MQ=255)
                     tgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtg                                                                                                                            >  2:4236267/1‑151 (MQ=255)
                      gaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtgg                                                                                                                           >  2:2431349/1‑151 (MQ=255)
                       aCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggc                                                                                                                          >  2:618904/1‑150 (MQ=255)
                        ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                                                                                                                         <  2:3729120/151‑1 (MQ=255)
                            aaGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCt                                                                                                                     >  2:580023/1‑151 (MQ=255)
                             aGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTa                                                                                                                    <  1:4783451/151‑1 (MQ=255)
                                   tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                                                                                                              >  1:597688/1‑151 (MQ=255)
                                   tGGAAGACCTGGCGAAGTGCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                                                                                                              <  2:1190935/151‑1 (MQ=255)
                                     gAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGa                                                                                                            >  1:5111146/1‑151 (MQ=255)
                                      aaGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGac                                                                                                           <  1:2552787/151‑2 (MQ=255)
                                       aGACCTGGCGAAGTTCCCCTTCACCGGCAAGCACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAAc                                                                                                          <  2:1667538/151‑1 (MQ=255)
                                       aGACCTGGCGAAGTTCCCCGTCACCGGCAAGAACGAGCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAAc                                                                                                          <  2:3875499/151‑1 (MQ=255)
                                        gACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACg                                                                                                         >  1:1810910/1‑151 (MQ=255)
                                                 aaGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGCGGTCGGCTACACCCAGAACCATATCGacc                                                                                                >  1:3910375/1‑150 (MQ=255)
                                                  aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGacac                                                                                               >  2:2265232/1‑151 (MQ=255)
                                                  aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGACTACACCCAGAACGATATCGacac                                                                                               >  2:4824552/1‑151 (MQ=255)
                                                    ttccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCt                                                                                             >  1:647763/3‑151 (MQ=255)
                                                    ttccccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCt                                                                                             <  1:2316218/149‑1 (MQ=255)
                                                       cccTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTggg                                                                                          <  1:3710512/151‑1 (MQ=255)
                                                         cTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGcc                                                                                        >  2:3852822/1‑151 (MQ=255)
                                                           tCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCaa                                                                                      >  1:3615356/1‑151 (MQ=255)
                                                           tCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCaa                                                                                      <  1:2146342/151‑1 (MQ=255)
                                                             aCCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtg                                                                                    >  1:1988538/1‑151 (MQ=255)
                                                               cGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAAtgtg                                                                                  <  1:80007/151‑1 (MQ=255)
                                                                  cAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGtt                                                                               <  1:2906609/151‑1 (MQ=255)
                                                                  cAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGtt                                                                               <  1:4872965/151‑1 (MQ=255)
                                                                   aaGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTg                                                                              >  2:896469/1‑151 (MQ=255)
                                                                       aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                                                                          <  2:2846160/151‑1 (MQ=255)
                                                                       aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                                                                          >  2:3126532/1‑151 (MQ=255)
                                                                       aCGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCg                                                                          <  2:80458/151‑1 (MQ=255)
                                                                        cGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGt                                                                         >  1:4702459/1‑151 (MQ=255)
                                                                         gaccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACATGGGCCAATGTGGCTGCCCGtt                                                                        >  2:412457/4‑151 (MQ=255)
                                                                          accTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTc                                                                       <  2:3699030/149‑1 (MQ=255)
                                                                           ccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTcc                                                                      <  1:5071636/150‑1 (MQ=255)
                                                                           ccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTcc                                                                      <  2:4629652/150‑1 (MQ=255)
                                                                           ccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTcc                                                                      >  1:4531956/2‑151 (MQ=255)
                                                                           ccTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTcc                                                                      <  1:564826/150‑1 (MQ=255)
                                                                               cgcgACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTc                                                                  <  1:4210186/151‑1 (MQ=255)
                                                                                  gACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTg                                                               <  2:2352976/151‑1 (MQ=255)
                                                                                   aCAACTACCCCTCCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGc                                                              <  1:4699407/151‑1 (MQ=255)
                                                                                   aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCgcgc                                                              >  2:4736158/1‑151 (MQ=255)
                                                                                   aCAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGc                                                              <  2:1395226/151‑1 (MQ=255)
                                                                                        tACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccg                                                         >  2:3068373/1‑151 (MQ=255)
                                                                                         cccccTCCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccgg                                                        <  1:3176257/150‑1 (MQ=255)
                                                                                         aCCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccgg                                                        >  2:1024638/1‑151 (MQ=255)
                                                                                         aCCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGcggccgg                                                        <  1:3822359/151‑1 (MQ=255)
                                                                                           cccTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCcggcg                                                      <  1:2565323/151‑1 (MQ=255)
                                                                                              tacGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCggcgggc                                                   >  2:3243566/3‑151 (MQ=255)
                                                                                              tacGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCggcgggc                                                   <  2:1753328/149‑1 (MQ=255)
                                                                                              tacGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCggcgggc                                                   <  1:3126532/149‑1 (MQ=255)
                                                                                              tacGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCggcgggc                                                   <  2:2640790/149‑1 (MQ=255)
                                                                                               acGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCggcgggcg                                                  <  1:3381786/150‑1 (MQ=255)
                                                                                                cGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGgcgc                                                 <  1:1511671/151‑1 (MQ=255)
                                                                                                  gCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCaa                                               <  1:5238515/151‑1 (MQ=255)
                                                                                                     tGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAgg                                            <  1:4430216/151‑1 (MQ=255)
                                                                                                       tttGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTg                                          <  2:2551018/151‑1 (MQ=255)
                                                                                                              tGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGt                                   <  1:909351/151‑1 (MQ=255)
                                                                                                              ggCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGt                                   <  2:4071807/150‑1 (MQ=255)
                                                                                                               gCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTg                                  <  1:946385/151‑1 (MQ=255)
                                                                                                                ccccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGc                                 >  1:4165266/1‑151 (MQ=255)
                                                                                                                 cccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCa                                <  2:597688/151‑1 (MQ=255)
                                                                                                                 cccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCa                                >  2:755012/1‑151 (MQ=255)
                                                                                                                  ccAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCAt                               <  1:9360/151‑1 (MQ=255)
                                                                                                                   cAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCAtg                              <  1:3335414/151‑1 (MQ=255)
                                                                                                                         gaagTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCct                        <  2:5147481/151‑1 (MQ=255)
                                                                                                                          aagTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCcta                       <  2:312181/151‑1 (MQ=255)
                                                                                                                           agTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctac                      <  1:2422856/151‑1 (MQ=255)
                                                                                                                             tggtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacgg                    >  2:486371/1‑151 (MQ=255)
                                                                                                                             tggtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacgg                    >  2:721131/1‑151 (MQ=255)
                                                                                                                              ggtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggc                   <  1:2465110/151‑1 (MQ=255)
                                                                                                                               gtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggct                  <  1:2077201/151‑1 (MQ=255)
                                                                                                                               gtgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggct                  <  2:3103784/151‑1 (MQ=255)
                                                                                                                                tgCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggcta                 >  1:1060879/1‑151 (MQ=255)
                                                                                                                                tgCGCCTGCATGCCTCCACTGGCACCACCGGCAAGCCGCCGGTGGTCCGCTACGCCCAGCACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCctacggcta                 <  2:3434658/151‑1 (MQ=255)
                                                                                                                                     cTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGc            >  2:18310/1‑151 (MQ=255)
                                                                                                                                       gCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTg          >  1:1660287/1‑151 (MQ=255)
                                                                                                                                        cATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAATGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGt         >  2:2151470/1‑151 (MQ=255)
                                                                                                                                        cATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGt         >  2:2739522/1‑151 (MQ=255)
                                                                                                                                         aTGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGtt        >  2:4373271/1‑151 (MQ=255)
                                                                                                                                         aTGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGtt        >  1:4607448/1‑151 (MQ=255)
                                                                                                                                          tGCCTCCAGTGGCACCACCGGCACGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTc       <  2:1704149/151‑1 (MQ=255)
                                                                                                                                          tGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTc       <  2:2301117/151‑1 (MQ=255)
                                                                                                                                          tGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTc       <  1:4941294/151‑1 (MQ=255)
                                                                                                                                            ccTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCAc     <  1:321443/151‑1 (MQ=255)
                                                                                                                                            ccTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCAc     >  1:3785215/1‑151 (MQ=255)
                                                                                                                                            ccTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCAc     >  1:3957394/1‑151 (MQ=255)
                                                                                                                                             cTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCTGAACGATATCGACACCTGGGCCACTGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCCTAGGTGACACGGTGCATGTGTCCTACGGCTACGCGCTGTTCAcc    >  2:3179526/1‑151 (MQ=255)
                                                                                                                                               ccAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACcgg  >  1:1968225/1‑151 (MQ=255)
                                                                                                                                               ccAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACcgg  >  2:126623/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTGGGCCAATGTGGTTGCCCGTTCCATTCGTGCGGCCGGCGGGCGCAAAGGTGACAAGGTGCATGTGTCCTACGGCTACGGGCTGTTCACCGG  >  CP009974/5007228‑5007521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑