BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,105,351	T→C	P167P (CCA→CCG)	RPPX_22650 ←	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,105,351	0	T	C	81.2%	144.8 / 47.3	112	P167P (CCA→CCG)	RPPX_22650	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base T (13/8); new base C (44/47); total (57/55)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 115 total aligned reads displayed.

GTGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCG  >  CP009974/5105201‑5105498

atgatAGCGGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                                                                                                                                     <  2:667921/147‑1 (MQ=1)

atgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                                                                                                                                     >  2:13944/5‑151 (MQ=1)

atgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                                                                                                                                     <  1:2636810/147‑1 (MQ=1)

 tgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTg                                                                                                                                                    >  2:1432336/4‑151 (MQ=1)

       ccGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTca                                                                                                                                              >  1:5075057/1‑151 (MQ=0)

                            gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                         >  1:150966/4‑151 (MQ=1)

                            gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                         >  1:3043802/4‑151 (MQ=1)

                            gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                         >  1:3043435/4‑151 (MQ=1)

                                       gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                              <  1:884125/151‑1 (MQ=1)

                                       gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                              <  1:3049541/151‑1 (MQ=1)

                                        ttaccTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGcccc                                                                                                             <  1:1110405/148‑1 (MQ=1)

                                            ctgcGTAGGTGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCAGAACAAAAAGCCCCGGTa                                                                                                         <  2:2022719/148‑1 (MQ=1)

                                                               agctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCAcc                                                                                      <  2:3887493/144‑1 (MQ=1)

                                                                gctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGACGCCCCGGTACGACTCGAATCGTGCCACCt                                                                                     <  1:1196736/145‑1 (MQ=1)

                                                                 ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                    <  1:3582024/146‑1 (MQ=1)

                                                                 ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                    >  1:3533153/6‑151 (MQ=1)

                                                                 ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                    <  1:792928/146‑1 (MQ=1)

                                                                                     ccgtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGTTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGGGCCGt                                                                >  1:2209783/7‑151 (MQ=1)

                                                                                      cgtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTc                                                               >  1:4543423/6‑151 (MQ=0)

                                                                                      cgtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTc                                                               >  2:3315559/6‑151 (MQ=0)

                                                                                       gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCg                                                              >  1:162744/5‑151 (MQ=0)

                                                                                       gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCg                                                              >  1:484598/5‑151 (MQ=0)

                                                                                       gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGCGCTCGACCAGTGGTTTCACATCGGCAGTCAGACTGCCCAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTCGGTCAGGCCCTGGGAGCCCTCg                                                              >  2:4275632/5‑151 (MQ=1)

                                                                                        tccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGc                                                             <  1:1442260/148‑1 (MQ=1)

                                                                                                 tGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                    >  2:445610/1‑151 (MQ=11)

                                                                                                  gATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                   >  2:3963779/1‑151 (MQ=11)

                                                                                                   aTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTg                                                  >  1:1745460/1‑151 (MQ=11)

                                                                                                     cggcgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTc                                                <  1:2794826/148‑1 (MQ=11)

                                                                                                     cgacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTc                                                <  1:252840/151‑1 (MQ=11)

                                                                                                      gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                               >  1:2962260/1‑151 (MQ=11)

                                                                                                        cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                   >  1:610701/1‑145 (MQ=11)

                                                                                                        cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                   <  2:610701/145‑1 (MQ=11)

                                                                                                        cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                             <  2:3062949/151‑1 (MQ=11)

                                                                                                        cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                             >  1:2116026/1‑151 (MQ=11)

                                                                                                         gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgggg                                            >  1:4409847/1‑151 (MQ=11)

                                                                                                          ttCATGTCGATGATCTTCGCGGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                           <  2:3926754/150‑1 (MQ=11)

                                                                                                          aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                           <  2:4176349/151‑1 (MQ=11)

                                                                                                          aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGCGt                                           >  2:1855308/1‑151 (MQ=11)

                                                                                                           tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          >  2:3771655/1‑151 (MQ=11)

                                                                                                           tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          <  1:1057282/151‑1 (MQ=11)

                                                                                                           tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          <  1:2460073/151‑1 (MQ=11)

                                                                                                           tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          <  1:1779292/151‑1 (MQ=11)

                                                                                                            cATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAg                                         >  2:1802813/1‑151 (MQ=11)

                                                                                                            cATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAg                                         >  1:1465485/1‑151 (MQ=11)

                                                                                                             aTGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGc                                        >  1:3658969/1‑151 (MQ=11)

                                                                                                              tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                       <  2:1504569/151‑1 (MQ=11)

                                                                                                              tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                       >  1:3363511/1‑151 (MQ=11)

                                                                                                               gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                      <  1:4467167/151‑1 (MQ=11)

                                                                                                               gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                      >  2:4378176/1‑151 (MQ=11)

                                                                                                                tCGATGATCTTCGCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                     <  2:2343368/151‑1 (MQ=11)

                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                     <  2:2343252/151‑1 (MQ=11)

                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCCTGGTCAGGTCGCGGGAGCCGTCGCAGTTCAAGCTGTCGGGGTAGCCgg                                     >  2:4227764/1‑151 (MQ=11)

                                                                                                                  gatgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCg                                   <  2:4488340/151‑1 (MQ=11)

                                                                                                                   atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTTGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  1:345842/1‑151 (MQ=11)

                                                                                                                   atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  2:3041955/1‑151 (MQ=11)

                                                                                                                   atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  2:922196/1‑151 (MQ=11)

                                                                                                                   atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  1:345807/1‑151 (MQ=11)

                                                                                                                   atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  1:2468429/1‑151 (MQ=11)

                                                                                                                    tgatCTTCGCCGCCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 <  1:4716245/151‑1 (MQ=11)

                                                                                                                    tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 <  1:4715976/151‑1 (MQ=11)

                                                                                                                    tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 <  1:4344474/151‑1 (MQ=11)

                                                                                                                      atCTTCGCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGc                               <  2:1824649/151‑1 (MQ=11)

                                                                                                                      atCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGc                               >  2:2289882/1‑151 (MQ=11)

                                                                                                                       tCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCt                              >  2:4641988/1‑151 (MQ=11)

                                                                                                                       ccTTCGCCGACTCGCCCAGGGGCTCGACCAGCGGCTTCGCATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCt                              <  2:1026810/150‑1 (MQ=11)

                                                                                                                           ggCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgggg                          <  2:1005725/150‑1 (MQ=11)

                                                                                                                           cGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgggg                          >  2:1180023/1‑151 (MQ=11)

                                                                                                                            gCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:2213650/151‑1 (MQ=11)

                                                                                                                            gCCGAGTGGCGCGGGTGCGCGACCGGCGGCTTGACATCGGCGTTCAGGCGGCCGAAGAAAAAGCCCCGGTACGACTCAAAACGGGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:4385034/151‑1 (MQ=2)

                                                                                                                            gCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:2213272/151‑1 (MQ=11)

                                                                                                                            gCCGACTCGCCCAGGTGCGCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:1596594/151‑1 (MQ=11)

                                                                                                                             ccGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTc                        >  2:1379919/1‑151 (MQ=11)

                                                                                                                              cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGCCCCGTCGCCGTTGAACCTGTCCGCGTCGCCGGCGTTGCTGGGGGCt                       >  2:2743638/1‑151 (MQ=11)

                                                                                                                              cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                       >  1:201183/1‑151 (MQ=11)

                                                                                                                              cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                       >  2:5089971/1‑151 (MQ=11)

                                                                                                                               gACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCtt                      <  2:1280656/151‑1 (MQ=11)

                                                                                                                               gACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCtt                      <  1:313509/151‑1 (MQ=11)

                                                                                                                                ccTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                     <  2:5070714/150‑1 (MQ=11)

                                                                                                                                aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                     >  1:1775858/1‑151 (MQ=11)

                                                                                                                                 cTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                    >  1:4064556/1‑151 (MQ=11)

                                                                                                                                  tCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCa                   >  1:4577164/1‑151 (MQ=11)

                                                                                                                                   cGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                  >  2:2923444/1‑151 (MQ=11)

                                                                                                                                     cccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCt                <  1:3104213/151‑1 (MQ=11)

                                                                                                                                      ccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCtt               <  1:4976132/151‑1 (MQ=14)

                                                                                                                                      ccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCtt               >  2:1604955/1‑151 (MQ=14)

                                                                                                                                        gggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTca             <  2:194868/150‑1 (MQ=14)

                                                                                                                                        aGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTca             <  1:1275797/151‑1 (MQ=14)

                                                                                                                                         ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag            <  2:1578873/151‑1 (MQ=14)

                                                                                                                                           tGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagca          >  2:3927121/1‑151 (MQ=14)

                                                                                                                                            gCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagcag         >  1:4013846/1‑151 (MQ=14)

                                                                                                                                             cTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGt        >  1:2374371/1‑151 (MQ=14)

                                                                                                                                              tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt       >  2:1380660/1‑151 (MQ=14)

                                                                                                                                               cGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGGGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCCGttt      >  1:4644516/1‑151 (MQ=14)

                                                                                                                                                gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg     >  1:3102984/1‑151 (MQ=14)

                                                                                                                                                 aCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGc    <  1:4897466/151‑1 (MQ=14)

                                                                                                                                                  ccAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGcc   <  2:4544586/151‑1 (MQ=14)

                                                                                                                                                  ccAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGcc   <  2:5075057/151‑1 (MQ=14)

                                                                                                                                                  ccAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGcc   <  2:1729317/151‑1 (MQ=14)

                                                                                                                                                   cAGCGGCTTCGCATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCg  <  2:3483636/151‑1 (MQ=14)

                                                                                                                                                   cAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCg  <  1:3401887/151‑1 (MQ=14)

GTGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCG  >  CP009974/5105201‑5105498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`