BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,105,354	T→C	K166K (AAA→AAG)	RPPX_22650 ←	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,105,354	0	T	C	82.0%	144.3 / 47.7	111	K166K (AAA→AAG)	RPPX_22650	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base T (12/8); new base C (44/47); total (56/55)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 111 total aligned reads displayed.

CCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCG  >  CP009974/5105208‑5105498

ccGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTca                                                                                                                                              >  1:5075057/1‑151 (MQ=0)

ccGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTca                                                                                                                                              >  1:4544586/1‑151 (MQ=0)

            ttctCGGCGATCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCa                                                                                                                                  >  2:2585471/4‑151 (MQ=1)

                     gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                         >  1:150966/4‑151 (MQ=1)

                     gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                         >  1:3043802/4‑151 (MQ=1)

                     gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                         >  1:3043435/4‑151 (MQ=1)

                                gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                              <  1:884125/151‑1 (MQ=1)

                                gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                              <  1:3049541/151‑1 (MQ=1)

                                 ttaccTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGcccc                                                                                                             <  1:1110405/148‑1 (MQ=1)

                                 ttaccTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGcccc                                                                                                             <  1:3706044/148‑1 (MQ=1)

                                     ctgcGTAGGTGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCAGAACAAAAAGCCCCGGTa                                                                                                         <  2:2022719/148‑1 (MQ=1)

                                                         gctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGACGCCCCGGTACGACTCGAATCGTGCCACCt                                                                                     <  1:1196736/145‑1 (MQ=1)

                                                          ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                    <  1:792928/146‑1 (MQ=1)

                                                          ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                    <  1:3582024/146‑1 (MQ=1)

                                                          ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                    >  1:3533153/6‑151 (MQ=1)

                                                                              ccgtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGTTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGGGCCGt                                                                >  1:2209783/7‑151 (MQ=1)

                                                                               cgtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTc                                                               >  1:4543423/6‑151 (MQ=0)

                                                                               cgtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTc                                                               >  2:3315559/6‑151 (MQ=0)

                                                                                gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCg                                                              >  1:162744/5‑151 (MQ=0)

                                                                                gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCg                                                              >  1:484598/5‑151 (MQ=0)

                                                                                gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGCGCTCGACCAGTGGTTTCACATCGGCAGTCAGACTGCCCAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTCGGTCAGGCCCTGGGAGCCCTCg                                                              >  2:4275632/5‑151 (MQ=1)

                                                                                 tccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGc                                                             <  1:1442260/148‑1 (MQ=1)

                                                                                   gggggACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAg                                                           <  2:3613312/147‑1 (MQ=0)

                                                                                           gATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                   >  2:3963779/1‑151 (MQ=11)

                                                                                            aTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTg                                                  >  1:1745460/1‑151 (MQ=11)

                                                                                              cgacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTc                                                <  1:252840/151‑1 (MQ=11)

                                                                                               gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                               >  1:2962260/1‑151 (MQ=11)

                                                                                                 cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                   >  1:610701/1‑145 (MQ=11)

                                                                                                 cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                   <  2:610701/145‑1 (MQ=11)

                                                                                                 cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                             >  1:2116026/1‑151 (MQ=11)

                                                                                                 cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                             <  2:3062949/151‑1 (MQ=11)

                                                                                                  gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgggg                                            >  1:4409847/1‑151 (MQ=11)

                                                                                                   ttCATGTCGATGATCTTCGCGGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                           <  2:3926754/150‑1 (MQ=11)

                                                                                                   aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                           <  2:4176349/151‑1 (MQ=11)

                                                                                                   aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGCGt                                           >  2:1855308/1‑151 (MQ=11)

                                                                                                    tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          <  1:1057282/151‑1 (MQ=11)

                                                                                                    tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          <  1:2460073/151‑1 (MQ=11)

                                                                                                    tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          >  2:3771655/1‑151 (MQ=11)

                                                                                                    tCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTa                                          <  1:1779292/151‑1 (MQ=11)

                                                                                                     cATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAg                                         >  1:1465485/1‑151 (MQ=11)

                                                                                                      aTGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGc                                        >  1:3658969/1‑151 (MQ=11)

                                                                                                       tGTCGATGATCTTCGCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                       <  2:1506321/151‑1 (MQ=11)

                                                                                                       tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                       <  2:1504569/151‑1 (MQ=11)

                                                                                                       tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                       >  1:3363408/1‑151 (MQ=11)

                                                                                                        gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                      <  1:4467167/151‑1 (MQ=11)

                                                                                                        gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                      >  2:4378176/1‑151 (MQ=11)

                                                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                     <  1:5035734/151‑1 (MQ=11)

                                                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                     <  2:2343252/151‑1 (MQ=11)

                                                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCCTGGTCAGGTCGCGGGAGCCGTCGCAGTTCAAGCTGTCGGGGTAGCCgg                                     >  2:4227764/1‑151 (MQ=11)

                                                                                                         tCGATGATCCTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                     <  2:4231015/151‑1 (MQ=11)

                                                                                                           gatgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCg                                   <  2:4488340/151‑1 (MQ=11)

                                                                                                            atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTTGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  1:345842/1‑151 (MQ=11)

                                                                                                            atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  2:922196/1‑151 (MQ=11)

                                                                                                            atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  2:3041955/1‑151 (MQ=11)

                                                                                                            atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  1:345807/1‑151 (MQ=11)

                                                                                                            atgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGt                                  >  1:2468429/1‑151 (MQ=11)

                                                                                                             tgatCTTCGCCGCCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 <  1:4716245/151‑1 (MQ=11)

                                                                                                             tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 <  1:4715976/151‑1 (MQ=11)

                                                                                                             tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 <  1:162535/151‑1 (MQ=11)

                                                                                                             tgatCTTCGCCGACTCGCCCAGCTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                 >  2:4654113/1‑151 (MQ=11)

                                                                                                               atCTTCGCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGc                               <  2:1824649/151‑1 (MQ=11)

                                                                                                               atCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGc                               >  2:2289882/1‑151 (MQ=11)

                                                                                                                tCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCt                              >  2:4641988/1‑151 (MQ=11)

                                                                                                                ccTTCGCCGACTCGCCCAGGGGCTCGACCAGCGGCTTCGCATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCt                              <  2:1026810/150‑1 (MQ=11)

                                                                                                                 cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                             <  1:2941509/151‑1 (MQ=11)

                                                                                                                    ggCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgggg                          <  2:1005725/150‑1 (MQ=11)

                                                                                                                    cGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgggg                          >  2:1180023/1‑151 (MQ=11)

                                                                                                                     gCCGGCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:2213650/151‑1 (MQ=11)

                                                                                                                     gCCGAGTGGCGCGGGTGCGCGACCGGCGGCTTGACATCGGCGTTCAGGCGGCCGAAGAAAAAGCCCCGGTACGACTCAAAACGGGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:4385034/151‑1 (MQ=2)

                                                                                                                     gCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:2213272/151‑1 (MQ=11)

                                                                                                                     gCCGACTCGCCCAGGTGCGCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                         <  2:1596594/151‑1 (MQ=11)

                                                                                                                      ccGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTc                        >  2:1379919/1‑151 (MQ=11)

                                                                                                                       cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGCCCCGTCGCCGTTGAACCTGTCCGCGTCGCCGGCGTTGCTGGGGGCt                       >  2:2743638/1‑151 (MQ=11)

                                                                                                                       cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                       >  2:5089971/1‑151 (MQ=11)

                                                                                                                       cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                       >  1:201183/1‑151 (MQ=11)

                                                                                                                        gACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCtt                      <  2:1280656/151‑1 (MQ=11)

                                                                                                                        gACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCtt                      <  1:313509/151‑1 (MQ=11)

                                                                                                                         ccTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                     <  2:5070714/150‑1 (MQ=11)

                                                                                                                          cTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                    >  1:4064556/1‑151 (MQ=11)

                                                                                                                           tCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCa                   >  1:4577164/1‑151 (MQ=11)

                                                                                                                            cGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                  >  2:2923444/1‑151 (MQ=11)

                                                                                                                              cccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCt                <  1:3104213/151‑1 (MQ=11)

                                                                                                                               ccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCtt               <  1:4976132/151‑1 (MQ=14)

                                                                                                                               ccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCtt               >  2:1604955/1‑151 (MQ=14)

                                                                                                                                 gggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTca             <  2:194868/150‑1 (MQ=14)

                                                                                                                                 aGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTca             <  1:1275797/151‑1 (MQ=14)

                                                                                                                                  ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag            <  2:1578873/151‑1 (MQ=14)

                                                                                                                                   gTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagc           >  1:2360990/1‑151 (MQ=14)

                                                                                                                                    tGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagca          >  2:3927121/1‑151 (MQ=14)

                                                                                                                                     gCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagcag         >  1:4013846/1‑151 (MQ=14)

                                                                                                                                      cTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGt        >  1:2374371/1‑151 (MQ=14)

                                                                                                                                       tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt       >  2:1380660/1‑151 (MQ=14)

                                                                                                                                        cGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGGGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCCGttt      >  1:4644516/1‑151 (MQ=14)

                                                                                                                                         gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg     >  1:3102984/1‑151 (MQ=14)

                                                                                                                                          aCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGc    <  2:1922833/151‑1 (MQ=14)

                                                                                                                                           ccAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGcc   <  2:4544586/151‑1 (MQ=14)

                                                                                                                                           ccAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGcc   <  2:5075057/151‑1 (MQ=14)

                                                                                                                                           ccAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGcc   <  2:1729317/151‑1 (MQ=14)

                                                                                                                                            cAGCGGCTTCGCATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCg  <  2:3483636/151‑1 (MQ=14)

                                                                                                                                            cAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCg  <  1:3401887/151‑1 (MQ=14)

CCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCG  >  CP009974/5105208‑5105498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`