Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,134,626 T→C T170A (ACG→GCG)  RPPX_22775 ← serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,134,6260TC88.9% 639.7 / 55.7 234T170A (ACG→GCG) RPPX_22775serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (10/16);  new base C (106/102);  total (116/118)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

AACCATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCGGTGCCGA  >  CP009974/5134477‑5134772
                                                                                                                                                     |                                                                                                                                                  
aaCCATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTg                                                                                                                                                   <  1:4799305/151‑1 (MQ=255)
 aCCATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCgg                                                                                                                                                  >  1:3624146/1‑151 (MQ=255)
  ccATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCggc                                                                                                                                                 >  2:3288514/1‑151 (MQ=255)
   cATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCgcggcg                                                                                                                                                <  1:3733377/151‑1 (MQ=255)
   cATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTggcg                                                                                                                                                >  2:313601/1‑151 (MQ=255)
           gAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCCCCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTg                                                                                                                                        <  1:1146005/151‑1 (MQ=255)
           gAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTg                                                                                                                                        <  2:4524651/151‑1 (MQ=255)
            aaCCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCCCGGCGGCCGGTTGc                                                                                                                                       >  2:3233817/1‑151 (MQ=255)
              cccGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCg                                                                                                                                     <  1:3554322/151‑1 (MQ=255)
                cGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCggg                                                                                                                                   <  1:537757/151‑1 (MQ=255)
                cGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCggg                                                                                                                                   <  1:3855912/151‑1 (MQ=255)
                cGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCggg                                                                                                                                   >  2:1697641/1‑151 (MQ=255)
                 gAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGc                                                                                                                                  >  1:5144286/1‑151 (MQ=255)
                    tCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCtt                                                                                                                               >  2:571120/1‑151 (MQ=255)
                       aCGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTtgat                                                                                                                            >  1:1478408/1‑151 (MQ=255)
                       aCGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGCTGCCGGGCCTtgat                                                                                                                            >  2:1827807/1‑151 (MQ=255)
                        cGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTtga                                                                                                                             >  2:3963203/1‑149 (MQ=255)
                         gTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTtgatga                                                                                                                          >  1:4384456/1‑151 (MQ=255)
                         gTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTtgatga                                                                                                                          >  1:3528345/1‑151 (MQ=255)
                         gTACATGACCTTGTCCGGCCCCGGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTtgatga                                                                                                                          <  2:1950045/151‑1 (MQ=255)
                              tGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGcc                                                                                                                     <  1:1733253/151‑1 (MQ=255)
                              tGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGcc                                                                                                                     <  1:4040394/151‑1 (MQ=255)
                                       ccGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCCTAGGCCTCACCCGACCGATGCGGCGGCGGCCGGCTGCCGCGCCGTGATGATGGCTGCCAccccc                                                                                                            >  2:3435565/1‑151 (MQ=255)
                                       ccGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCAccccc                                                                                                            <  2:2305787/151‑1 (MQ=255)
                                        cGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCa                                                                                                           <  1:2197064/151‑1 (MQ=255)
                                            cccTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGc                                                                                                       >  2:758980/1‑151 (MQ=255)
                                             ccTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGcc                                                                                                      >  1:2634251/1‑151 (MQ=255)
                                                 cgcCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTg                                                                                                  <  1:1094951/151‑1 (MQ=255)
                                                  gcCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGa                                                                                                 >  2:3626761/1‑151 (MQ=255)
                                                  gcCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGa                                                                                                 <  2:4253867/151‑1 (MQ=255)
                                                   ccAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGaa                                                                                                >  2:1071882/1‑151 (MQ=255)
                                                     aGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAAtt                                                                                              >  1:760866/1‑151 (MQ=255)
                                                     aGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAAtt                                                                                              >  2:376429/1‑151 (MQ=255)
                                                     aGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAAtt                                                                                              <  1:298169/151‑1 (MQ=255)
                                                         aaaCAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTcc                                                                                          >  2:303686/1‑151 (MQ=255)
                                                         aaaCAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTcc                                                                                          <  2:2482863/151‑1 (MQ=255)
                                                            cAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCCTAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGACTTGTCCGGc                                                                                       >  2:1342090/1‑151 (MQ=255)
                                                               ggCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGcaca                                                                                    >  2:678152/1‑151 (MQ=255)
                                                                 cAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATc                                                                                  <  2:1020693/151‑1 (MQ=255)
                                                                      cTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCa                                                                             <  1:2726622/151‑1 (MQ=255)
                                                                       tCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAg                                                                            <  1:3254908/151‑1 (MQ=255)
                                                                       tCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAg                                                                            <  2:493978/151‑1 (MQ=255)
                                                                        cGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGTTGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGc                                                                           <  1:2263145/151‑1 (MQ=255)
                                                                         gagaCGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCa                                                                          >  1:4344025/1‑151 (MQ=255)
                                                                          agaCGCTGGCCTTCATTGCGGCCGATCACCCCCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCac                                                                         <  2:4709760/151‑1 (MQ=255)
                                                                          agaCGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCac                                                                         <  1:3089774/151‑1 (MQ=255)
                                                                          agaCGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCac                                                                         <  2:3503776/151‑1 (MQ=255)
                                                                                 ggCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCa                                                                  <  1:138358/151‑1 (MQ=255)
                                                                                  gCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCAt                                                                 <  1:895299/151‑1 (MQ=255)
                                                                                     ttCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTgg                                                              >  1:677138/1‑151 (MQ=255)
                                                                                      tCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGgc                                                             <  2:1688016/151‑1 (MQ=255)
                                                                                      tCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGCCCGCTTTCGGTGCCTGGACGCAGTCCGGGCACCCGACTCCCGCGGAACGGTCCGGCGCAACTACGACGACGACGGCATGGgc                                                             >  2:3857223/1‑151 (MQ=255)
                                                                                       cATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGgcg                                                            >  2:459579/1‑151 (MQ=255)
                                                                                        aTTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGgcgc                                                           <  1:1949255/151‑1 (MQ=255)
                                                                                         ttGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGcc                                                          <  2:2201959/151‑1 (MQ=255)
                                                                                           gCGGCCGATCGCCACCACGCCGATCACCGTTGCGATAGGCCTCGCTCGACCGAGGCCGCGGCGGCCGGTTGCCGGGCCGTGATGATGGCCGGCCCCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCga                                                        <  2:2153184/151‑1 (MQ=255)
                                                                                            cGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCgat                                                       <  1:3033534/151‑1 (MQ=255)
                                                                                             ggCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCgatg                                                      >  1:1841382/1‑151 (MQ=255)
                                                                                              gCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCgatga                                                     <  1:2936119/151‑1 (MQ=255)
                                                                                                cGATCACCACCACGCCGATCACCGTTGCCCTAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCCTTGGCGCCgatgatt                                                   >  2:5354/1‑150 (MQ=255)
                                                                                                cGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATc                                                   >  1:4579876/1‑151 (MQ=255)
                                                                                                cGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATc                                                   >  2:5087/1‑151 (MQ=255)
                                                                                                     cccaccccGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACgtt                                              <  2:4076979/150‑1 (MQ=255)
                                                                                                      ccaccacGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGTCGGCATTGGCGCCGATGATCACgttg                                             >  1:5019406/1‑151 (MQ=255)
                                                                                                      ccaccacGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACgttg                                             <  1:1290234/151‑1 (MQ=255)
                                                                                                         ccacGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGttgttg                                          >  1:4085021/1‑151 (MQ=255)
                                                                                                         ccacGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGttcttg                                          >  2:2972012/1‑151 (MQ=255)
                                                                                                           acGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGcc                                        <  2:4498783/151‑1 (MQ=255)
                                                                                                            cGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCg                                       <  1:3465828/151‑1 (MQ=255)
                                                                                                            cGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCg                                       <  1:1007956/151‑1 (MQ=255)
                                                                                                              ccGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGAt                                     >  2:2411824/1‑151 (MQ=255)
                                                                                                               cGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATg                                    >  1:469681/1‑151 (MQ=255)
                                                                                                                gATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGc                                   >  1:282846/1‑151 (MQ=255)
                                                                                                                gATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCGTGATGATGGCCGGCACCCCCACTGCCAGGGAATGGTCCGGCACATCTACCAGCTCGTCGGCATTGGCGCCGATTTTCAGGTTTTTGCCGATgg                                   >  2:1752863/1‑150 (MQ=255)
                                                                                                                   cACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGaa                                <  1:1411512/151‑1 (MQ=255)
                                                                                                                   cACCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGaa                                <  2:4052691/151‑1 (MQ=255)
                                                                                                                    aCCGTTGCCATAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAAt                               <  1:3141446/151‑1 (MQ=255)
                                                                                                                    aCCGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGaaa                               >  2:4133673/1‑150 (MQ=255)
                                                                                                                      cGTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATAc                             <  1:473621/151‑1 (MQ=255)
                                                                                                                       gTTGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACt                            <  2:1379418/151‑1 (MQ=255)
                                                                                                                        ttGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTg                           <  2:1283853/151‑1 (MQ=255)
                                                                                                                         tGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGCATACTGc                          >  2:5212757/1‑151 (MQ=255)
                                                                                                                         tGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGc                          <  2:4939246/151‑1 (MQ=255)
                                                                                                                         tGCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGc                          >  1:4264586/1‑151 (MQ=255)
                                                                                                                          gCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGcc                         <  1:4903541/151‑1 (MQ=255)
                                                                                                                          gCCATAGGCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGcc                         >  1:3400500/1‑151 (MQ=255)
                                                                                                                             aTAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGCTGATCACGTTGTTGCCGATGCGAATACTGCCAAg                      >  2:3557955/1‑151 (MQ=255)
                                                                                                                                 gCCTCACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCAcc                  >  1:4873314/1‑151 (MQ=255)
                                                                                                                                  ccTCACTCGACCGATGCCGCGGCGGCCCGTTGCCGGGCCTTGATGATGGCCGGCGCCCCCACTGCCAGTGACTTGTCTGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTCTTGCCGATGCGCATACTCCCCAGCAccc                 >  2:4585962/1‑150 (MQ=255)
                                                                                                                                   cTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCtt                <  2:1392953/151‑1 (MQ=255)
                                                                                                                                     cACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTgg              >  1:3806200/1‑151 (MQ=255)
                                                                                                                                     cACTCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTgg              <  2:2924117/151‑1 (MQ=255)
                                                                                                                                        tCGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTTGccc           >  1:1589669/1‑151 (MQ=255)
                                                                                                                                         cGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGcccc          >  2:4450280/1‑151 (MQ=255)
                                                                                                                                         cGACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGcccc          <  1:4108500/151‑1 (MQ=255)
                                                                                                                                          gACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCg         <  1:85014/151‑1 (MQ=255)
                                                                                                                                          gACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCg         >  1:1059415/1‑151 (MQ=255)
                                                                                                                                          gACCGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCg         <  2:2145383/151‑1 (MQ=255)
                                                                                                                                             cGATGCCGCGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCGGTg      <  1:2778600/151‑1 (MQ=255)
                                                                                                                                              gATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCGGTGc     <  2:1056197/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AACCATCGGTGGAACCCGAGTCGACGTACATGACCTTGTCCGGCCCCTGCGCCAGCGAAACAAGGCAGCGCTCGAGACGCTGGCCTTCATTGCGGCCGATCACCACCACGCCGATCACCGTTGCCATAGGCCTCACTCGACCGATGCCGTGGCGGCCGGTTGCCGGGCCTTGATGATGGCCGGCACCCCCACTGCCAGTGAATTGTCCGGCACATCTACCAGCACGACGGCATTGGCGCCGATGATCACGTTGTTGCCGATGCGAATACTGCCCAGCACCTTGGCCCCGGTGCCGA  >  CP009974/5134477‑5134772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑