Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,154,888 T→C V78A (GTC→GCC)  RPPX_22865 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,154,8880TC99.2% 861.5 / ‑3.6 247V78A (GTC→GCC) RPPX_22865oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (114/131);  total (116/131)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 247 total aligned reads displayed.

GCTGCCGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGTCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCGAGCGCT  >  CP009974/5154740‑5155034
                                                                                                                                                    |                                                                                                                                                  
gCTGCCGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCt                                                                                                                                                  <  2:4346338/151‑1 (MQ=255)
gCTGCCGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCt                                                                                                                                                  <  1:1419041/151‑1 (MQ=255)
   gCCGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTAcc                                                                                                                                               >  2:2010205/1‑151 (MQ=255)
   gCCGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTAcc                                                                                                                                               >  1:1124794/1‑151 (MQ=255)
    ccGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTAccc                                                                                                                                              <  2:838941/151‑1 (MQ=255)
      gAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGc                                                                                                                                            <  2:2964677/151‑1 (MQ=255)
        aGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGTCACCGCCGTGTTGTATGCCGTAGGTGGGGCTGTAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGTGACCAGGTTTTTCTGTTCAGCTAGGCCTTCTCGcac                                                                                                                                          >  2:887531/1‑151 (MQ=255)
         gTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGcaca                                                                                                                                         <  1:4535734/151‑1 (MQ=255)
         gTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGcaca                                                                                                                                         <  1:2562833/151‑1 (MQ=255)
             cggcgCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGGATGCCGAAGGGGGGGGAGAAGAGGCCGTGGGCCAAGCCATCGCCGGGCGCGGCGACCACGTATCTCTGGCCAGCAAGGCCTACCCGCACAATGc                                                                                                                                     >  2:3946619/1‑151 (MQ=255)
              ggcgCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGcc                                                                                                                                    >  2:4936041/1‑151 (MQ=255)
                cgcTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCgg                                                                                                                                  <  1:726097/151‑1 (MQ=255)
                    gCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTg                                                                                                                              >  2:1005794/1‑151 (MQ=255)
                     cAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCGAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTgc                                                                                                                             <  2:264812/151‑1 (MQ=255)
                     cAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTgc                                                                                                                             <  1:1284964/151‑1 (MQ=255)
                      aaCAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTgcg                                                                                                                            <  1:2737668/151‑1 (MQ=255)
                       aCAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTgcgc                                                                                                                           <  1:1548662/151‑1 (MQ=255)
                         aGGGCATCGAGCTGGGCCTGAACTTGTTCGACACCCCGCAGACGTCTGGAGAAGCGCGCGCACCAGAGGCCGCTCGCCTAGCCATTGCCGGGCCCTGCGACCAGCTATTGCTGGCCAGCAAGGCCTACACGGACGATGGCGGCCTGCGCgg                                                                                                                         <  2:3551383/151‑1 (MQ=255)
                             aaTCGAGATGAGACTAAACCTGATCGAAACCGGCGAGATGTATGACGAAGGGGGGGCAGAAGAGGTCGTTGGCCATGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATg                                                                                                                     <  2:2275576/150‑1 (MQ=255)
                              aTCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATgg                                                                                                                    <  2:1649199/151‑1 (MQ=255)
                               tCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATggc                                                                                                                   <  1:4351410/151‑1 (MQ=255)
                                 gAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATggcgg                                                                                                                 >  2:3012780/1‑151 (MQ=255)
                                 gAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATggcgg                                                                                                                 <  1:245865/151‑1 (MQ=255)
                                 gAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATggcgg                                                                                                                 >  2:2353244/1‑151 (MQ=255)
                                   gCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGct                                                                                                               >  1:3079561/1‑151 (MQ=255)
                                   gCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGct                                                                                                               >  1:4999967/1‑151 (MQ=255)
                                         cctgatcctgatCGACGCCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGAACAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGctgcctgc                                                                                                         <  1:4483869/151‑1 (MQ=255)
                                          cTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCg                                                                                                        >  1:4842878/1‑151 (MQ=255)
                                             aaCCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAAc                                                                                                     <  1:4890454/151‑1 (MQ=255)
                                              aCCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACg                                                                                                    <  1:2073804/151‑1 (MQ=255)
                                                 tGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAg                                                                                                 >  1:509000/1‑151 (MQ=255)
                                                     cggcacCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTc                                                                                             <  1:4305647/148‑1 (MQ=255)
                                                     cGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTc                                                                                             <  1:825736/151‑1 (MQ=255)
                                                     cGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTc                                                                                             <  1:4804225/151‑1 (MQ=255)
                                                     cGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTc                                                                                             >  2:295095/1‑151 (MQ=255)
                                                      gACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGACCGCAGCCTcc                                                                                            >  1:3276736/1‑150 (MQ=255)
                                                      gACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTca                                                                                            >  1:421104/1‑151 (MQ=255)
                                                      gACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTca                                                                                            <  2:4279740/151‑1 (MQ=255)
                                                          ccgccgAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACg                                                                                        <  1:3873972/151‑1 (MQ=255)
                                                          ccgccgAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACg                                                                                        >  2:5198119/1‑151 (MQ=255)
                                                            gccgAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGcc                                                                                      >  2:4040328/1‑151 (MQ=255)
                                                                agaTGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGTCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCCGCCCGCGACCCCAGCCTCCCACGCCTgcg                                                                                  >  2:779797/1‑149 (MQ=255)
                                                                 gaTGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGc                                                                                 >  2:4628448/1‑151 (MQ=255)
                                                                   tGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCAc                                                                               >  1:4446352/1‑151 (MQ=255)
                                                                      aTGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGa                                                                            >  2:257472/1‑151 (MQ=255)
                                                                         ccGGAGGGGGGGCAGAAGAGGGCGTTGGCCAAGCCATTGCCGGGCGCGGCGGCCAGGTATTTCTGGTCAGCAAGGCCGACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTg                                                                         <  1:2130785/151‑1 (MQ=255)
                                                                         ccGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTg                                                                         <  1:2248756/151‑1 (MQ=255)
                                                                         ccGAAGGGGGGGCAGAAGAGGTCGGTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTg                                                                         <  2:1595470/151‑1 (MQ=255)
                                                                          cGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGc                                                                        >  1:1586499/1‑151 (MQ=255)
                                                                           gAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCa                                                                       <  2:503782/151‑1 (MQ=255)
                                                                           gAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCa                                                                       >  2:110789/1‑151 (MQ=255)
                                                                             aGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATc                                                                     <  1:3615151/151‑1 (MQ=255)
                                                                                   ggCAGAATAGGTCGTTGGCCAGGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAGGGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTg                                                               <  1:2621702/151‑1 (MQ=255)
                                                                                   ggCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTg                                                               <  1:3403994/151‑1 (MQ=255)
                                                                                   ggCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATGTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGGCCTg                                                               >  2:1214606/1‑151 (MQ=255)
                                                                                     cAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGTACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCCCACGCCTGGGCACTGATTGCATCGACCTGTa                                                             >  1:1354491/1‑151 (MQ=255)
                                                                                      agaagaGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTAc                                                            >  1:27846/1‑151 (MQ=255)
                                                                                       gaagaGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTAcc                                                           <  2:3163254/151‑1 (MQ=255)
                                                                                       gaagaGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTAcc                                                           >  1:2954105/1‑151 (MQ=255)
                                                                                       gaagaGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATAGACCTGTAcc                                                           <  1:5030380/151‑1 (MQ=255)
                                                                                        aagaGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACct                                                          <  1:1208864/151‑1 (MQ=255)
                                                                                          gagGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTCTACctgc                                                        >  2:3217326/1‑151 (MQ=255)
                                                                                             gTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCtgctgc                                                     >  1:3844339/1‑151 (MQ=255)
                                                                                               cGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCAc                                                   <  1:1446980/151‑1 (MQ=255)
                                                                                                 ttGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTg                                                 >  2:1794243/1‑151 (MQ=255)
                                                                                                    gCCGAGCTATTGCGGGGCGTCGCGACCAGGTATGTCTGGTCAGCACGGCATACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGAACCTGCTGCACTGGCg                                              <  2:3402408/151‑1 (MQ=255)
                                                                                                     ccAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGt                                             <  1:4685691/151‑1 (MQ=255)
                                                                                                       aaGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTgg                                           >  1:3715161/1‑151 (MQ=255)
                                                                                                       aaGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTgg                                           <  1:3772320/151‑1 (MQ=255)
                                                                                                         gCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTc                                         >  2:3483368/1‑151 (MQ=255)
                                                                                                         gCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTc                                         <  2:2196237/151‑1 (MQ=255)
                                                                                                          ccATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCa                                        <  2:1363588/151‑1 (MQ=255)
                                                                                                           cATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAg                                       <  2:5163181/151‑1 (MQ=255)
                                                                                                               gCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCAcc                                   >  2:2018355/1‑151 (MQ=255)
                                                                                                                ccGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCAccc                                  <  2:2495016/151‑1 (MQ=255)
                                                                                                                 ggggCGCCGCGACCAGGTATTTCTGGTCAGCAAGGCCGACCCGCACAACGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCCCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCg                                 <  2:2256317/150‑1 (MQ=255)
                                                                                                                     cgccgcGACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGt                                             <  1:2016577/135‑1 (MQ=255)
                                                                                                                           gACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGaaa                       >  1:1394709/1‑151 (MQ=255)
                                                                                                                           gACCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGaaa                       >  1:1981383/1‑151 (MQ=255)
                                                                                                                            aCCAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGCCAGCACCCGCCGGAGGAAAc                      >  2:1399202/1‑151 (MQ=255)
                                                                                                                              cAGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCg                    >  1:858224/1‑151 (MQ=255)
                                                                                                                               aGGTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGt                   <  2:1578491/151‑1 (MQ=255)
                                                                                                                                ggTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTc                  >  2:2181347/1‑151 (MQ=255)
                                                                                                                                ggTATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTc                  >  2:1713412/1‑151 (MQ=255)
                                                                                                                                  tATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCTGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGa                <  2:3275195/151‑1 (MQ=255)
                                                                                                                                  tATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGa                <  2:4541518/151‑1 (MQ=255)
                                                                                                                                  tATTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGa                <  2:1390656/151‑1 (MQ=255)
                                                                                                                                   aTTTCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAg               <  1:2518114/151‑1 (MQ=255)
                                                                                                                                    tttCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAgg              >  2:668430/1‑151 (MQ=255)
                                                                                                                                      tCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCGTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGc            <  2:276679/151‑1 (MQ=255)
                                                                                                                                      tCTGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGc            >  1:596034/1‑151 (MQ=255)
                                                                                                                                        tGGTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAACCCGTCCAGGGCtc          >  2:3586481/1‑150 (MQ=255)
                                                                                                                                         ggTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCCTGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGCCCTGTACCTGCTGCACTGGCGCGGTCAGCACCCGCTGGAGGACACCGTCGAgggggtt         >  2:4812839/1‑147 (MQ=255)
                                                                                                                                         ggTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTc         <  1:1794243/151‑1 (MQ=255)
                                                                                                                                          gTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCg        <  1:404648/151‑1 (MQ=255)
                                                                                                                                          gTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCg        <  2:1876363/151‑1 (MQ=255)
                                                                                                                                          gTCAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCg        >  1:189415/1‑151 (MQ=255)
                                                                                                                                            cAGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGACTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGCCCTGTACCTCCCGCGCTGCCGTCGGCAGCACCCGCTGGCGGCAACCGTCGTGGGCGACGAg      >  2:3765950/1‑151 (MQ=255)
                                                                                                                                             aGCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCGAgc     <  2:565265/151‑1 (MQ=255)
                                                                                                                                              gCAAGGCCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCGAgcg    <  1:5198030/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCTGCCGAAGTCGCGGCGCTGCAACAGGGCATCGAGCTGGGCCTGAACCTGATCGACACCGCCGAGATGTATGCCGAAGGGGGGGCAGAAGAGGTCGTTGGCCAAGCCATTGCCGGGCGCCGCGACCAGGTATTTCTGGTCAGCAAGGTCTACCCGCACAATGCCGGCCTGCGCGGCATGGCGGCTGCCTGCGAACGCAGCCTCACACGCCTGGGCACTGATTGCATCGACCTGTACCTGCTGCACTGGCGTGGTCAGCACCCGCTGGAGGAAACCGTCGAGGGCTTCGAGCGCT  >  CP009974/5154740‑5155034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑