Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,417,945 G→A S870S (AGC→AGT)  RPPX_24130 ← pyridoxalphosphate dependent aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,417,9450GA98.6% 1000.5 / NA 285S870S (AGC→AGT) RPPX_24130pyridoxalphosphate dependent aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (142/134);  total (146/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

TCATGCCAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGGCTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCGAGATCGTTG  >  CP009974/5417797‑5418092
                                                                                                                                                    |                                                                                                                                                   
tCATGCCAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACt                                                                                                                                                   <  2:4940105/151‑1 (MQ=255)
 cATGCCAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTc                                                                                                                                                  <  2:1071240/151‑1 (MQ=255)
  aTGCCAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCg                                                                                                                                                 >  1:4812263/1‑151 (MQ=255)
  aTGCCAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCg                                                                                                                                                 >  1:2222201/1‑151 (MQ=255)
     ccAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCa                                                                                                                                              >  2:4745491/1‑151 (MQ=255)
       aGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAgg                                                                                                                                            >  2:2290750/1‑151 (MQ=255)
       aGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAgg                                                                                                                                            <  1:1600874/151‑1 (MQ=255)
       aGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAgg                                                                                                                                            <  1:487784/151‑1 (MQ=255)
         aCAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTc                                                                                                                                          <  1:3163891/151‑1 (MQ=255)
           aaGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCtt                                                                                                                                        <  1:968245/151‑1 (MQ=255)
            aGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTg                                                                                                                                       >  2:1336136/1‑151 (MQ=255)
             gCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGa                                                                                                                                      <  2:859168/151‑1 (MQ=255)
                cATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATgg                                                                                                                                   >  1:357337/1‑151 (MQ=255)
                  tCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGAc                                                                                                                                 >  2:5103186/1‑151 (MQ=255)
                   cAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACg                                                                                                                                >  1:2319343/1‑151 (MQ=255)
                   cAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACg                                                                                                                                <  1:1872114/151‑1 (MQ=255)
                    aaCACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGt                                                                                                                               >  1:3793335/1‑151 (MQ=255)
                      cacCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTc                                                                                                                             <  1:4114985/151‑1 (MQ=255)
                      cacCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTc                                                                                                                             <  2:2421117/151‑1 (MQ=255)
                       acCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCa                                                                                                                            >  1:339705/1‑151 (MQ=255)
                          gACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCg                                                                                                                         >  1:1270038/1‑151 (MQ=255)
                          gACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCg                                                                                                                         <  1:5072537/151‑1 (MQ=255)
                               ggCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACg                                                                                                                    <  2:4514723/151‑1 (MQ=255)
                                    tGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGat                                                                                                               <  2:1949356/151‑1 (MQ=255)
                                         gCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGc                                                                                                          >  1:3505663/1‑151 (MQ=255)
                                            tACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGa                                                                                                       >  1:4981869/1‑151 (MQ=255)
                                             aCAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGAt                                                                                                      <  2:103294/151‑1 (MQ=255)
                                              cAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATc                                                                                                     <  2:2460243/151‑1 (MQ=255)
                                                 cTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACg                                                                                                  <  2:3466404/151‑1 (MQ=255)
                                                  tGAGCTTTCTTGAGATAGGCCCGCACGTTGGGCGCCAGGCGGTCGTCGAACGGCGTCTGGACGACGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCCCGGCGATATCGCAGATCACGc                                                                                                 <  2:1150443/151‑1 (MQ=255)
                                                   gACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCt                                                                                                <  1:4351455/151‑1 (MQ=255)
                                                    aCCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTg                                                                                               <  2:5143271/151‑1 (MQ=255)
                                                     ccTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTgc                                                                                              >  1:4444411/1‑151 (MQ=255)
                                                     ccTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTgc                                                                                              >  1:2525738/1‑151 (MQ=255)
                                                       ttttttCAGGTAGGCCCGCCCGTAGGGCGCCAGGCCGCCCTCGAACGGCCTCTGGACGATGCCCCCGTGCATGTAGAGCCCGGCTTCGCGTCGGCTCGCCAGCTCTTGATGGACGTTCAGCGGCACCGCGATATCGCAGAGCACGccccgc                                                                                            >  2:3250482/1‑146 (MQ=255)
                                                        tttttCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCt                                                                                           >  1:1962102/1‑151 (MQ=255)
                                                        tttttCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCt                                                                                           >  2:3331669/1‑151 (MQ=255)
                                                          tttCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCTAACGGCGTCTGGACGATGCCGCCGTGCATGTAGATCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCg                                                                                         >  1:847907/1‑151 (MQ=255)
                                                            tcagtgaGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGc                                                                                       <  1:3315975/145‑1 (MQ=255)
                                                            tCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTGCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCCGc                                                                                       >  2:2851346/1‑151 (MQ=255)
                                                            tCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGGGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGc                                                                                       <  2:4772386/151‑1 (MQ=255)
                                                             cAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGATCGGCGTCTGGACGATGCCGCCGTGCATGTAGAGTACGTCTTCGCGTTGTCTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCg                                                                                      >  2:1203456/1‑151 (MQ=255)
                                                             cAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCg                                                                                      <  1:2920922/151‑1 (MQ=255)
                                                                aTAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGaaa                                                                                   >  1:53591/1‑151 (MQ=255)
                                                                aTAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGaaa                                                                                   <  2:1510966/151‑1 (MQ=255)
                                                                aTAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGaaa                                                                                   >  1:4185060/1‑151 (MQ=255)
                                                                  aGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATg                                                                                 >  1:2106891/1‑151 (MQ=255)
                                                                        gCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGc                                                                           <  2:3154346/151‑1 (MQ=255)
                                                                          aCGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCAc                                                                         >  2:2189088/1‑151 (MQ=255)
                                                                          aCGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCAc                                                                         >  1:4511280/1‑151 (MQ=255)
                                                                            gTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCg                                                                       >  2:3847246/1‑151 (MQ=255)
                                                                            gTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCg                                                                       >  2:766070/1‑151 (MQ=255)
                                                                               gggCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAgg                                                                    >  1:1822270/1‑151 (MQ=255)
                                                                                  cgcCAGGCCGTCGTCTAACGGCGTCGGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAAc                                                                 <  2:3790644/151‑1 (MQ=255)
                                                                                  cgcCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAAc                                                                 >  1:889980/1‑151 (MQ=255)
                                                                                  cgcCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAAc                                                                 <  1:1442107/151‑1 (MQ=255)
                                                                                   gcCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACg                                                                <  1:4286180/151‑1 (MQ=255)
                                                                                   gcCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACg                                                                <  2:4634613/151‑1 (MQ=255)
                                                                                   gcCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACg                                                                <  1:2836976/151‑1 (MQ=255)
                                                                                    ccAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACgg                                                               >  2:3625158/1‑151 (MQ=255)
                                                                                      aGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCt                                                             <  1:1105967/151‑1 (MQ=255)
                                                                                       ggCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTg                                                            <  2:3271697/151‑1 (MQ=255)
                                                                                        gCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGa                                                           >  2:4685703/1‑151 (MQ=255)
                                                                                                  aaCGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTgg                                                 >  2:3337235/1‑151 (MQ=255)
                                                                                                   aCGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGc                                                >  2:626827/1‑151 (MQ=255)
                                                                                                   aCGGCGTCGGGACGATGCCGGCGTGCATGTAGAGCACGTCATCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGc                                                <  2:4005290/151‑1 (MQ=255)
                                                                                                    cGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCa                                               <  1:2785390/151‑1 (MQ=255)
                                                                                                      gCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAgc                                             <  1:675065/151‑1 (MQ=255)
                                                                                                       cGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAgcg                                            <  2:4883005/151‑1 (MQ=255)
                                                                                                        gTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGCGGAACGGCTGAGGCGCCTTGGCAgcgc                                           >  2:5224747/1‑151 (MQ=255)
                                                                                                        gTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGCCAGGTCTTGATGGACGTTCAGCGGCCCGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAgcgc                                           >  2:682046/1‑151 (MQ=255)
                                                                                                         tCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGca                                          >  2:975566/1‑151 (MQ=255)
                                                                                                         tCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGca                                          >  1:1522228/1‑151 (MQ=255)
                                                                                                         tCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGca                                          <  1:2462974/151‑1 (MQ=255)
                                                                                                           tGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACGCGGCAGGTCTTGATGGACGTGCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGcaca                                        <  2:3906461/151‑1 (MQ=255)
                                                                                                            ggACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAg                                       >  1:1770912/1‑151 (MQ=255)
                                                                                                                gATGCCGCCGGGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACa                                   <  2:2313312/151‑1 (MQ=255)
                                                                                                                    ccgccgTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATcc                               >  1:3219625/1‑151 (MQ=255)
                                                                                                                        cgTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTgc                           <  1:1008860/151‑1 (MQ=255)
                                                                                                                        cgTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTgc                           >  2:268816/1‑151 (MQ=255)
                                                                                                                        cgTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTgc                           >  2:418812/1‑151 (MQ=255)
                                                                                                                         gTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTgcg                          <  1:3547949/151‑1 (MQ=255)
                                                                                                                         gTGCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCCTgcg                          >  2:3013350/1‑151 (MQ=255)
                                                                                                                           gCATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCAGTGCGcc                        >  2:3202737/1‑151 (MQ=255)
                                                                                                                            cATGTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGccc                       <  2:339705/151‑1 (MQ=255)
                                                                                                                              ggtggagCACGTCTTCGCGTTGCCTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCtt                     <  2:1831664/147‑1 (MQ=255)
                                                                                                                               gTAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTg                    >  1:51932/1‑151 (MQ=255)
                                                                                                                                tAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGa                   >  1:3116663/1‑151 (MQ=255)
                                                                                                                                tAGAGCACGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGa                   >  1:3690764/1‑151 (MQ=255)
                                                                                                                                aagagCACGTCTTCGCGGTGACGCGGCAGGGCTTGATGGACGTACAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGa                   <  2:1724703/150‑1 (MQ=255)
                                                                                                                                      aCGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGt             >  1:2632466/1‑151 (MQ=255)
                                                                                                                                      aCGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGt             >  2:1755403/1‑151 (MQ=255)
                                                                                                                                       cGTCTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTc            <  2:704171/151‑1 (MQ=255)
                                                                                                                                          cTTCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCgag         >  1:2713338/1‑151 (MQ=255)
                                                                                                                                           ttCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCgaga        >  2:1587793/1‑151 (MQ=255)
                                                                                                                                           ttCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCgaga        >  1:3923968/1‑151 (MQ=255)
                                                                                                                                           ttCGCGTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCgaga        <  1:4639267/151‑1 (MQ=255)
                                                                                                                                            tCGCGTTGGCTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGCGCGCCCTTGATGGCGTCGAGAt       <  2:840413/151‑1 (MQ=255)
                                                                                                                                              gcgTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCGAGATCg     <  2:1282654/151‑1 (MQ=255)
                                                                                                                                                gTTGACTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCGAGATCGtt   >  2:2115473/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCATGCCAGACAAGCCCATCAACACCGACTCGGCCATGCACGCATACAGCTGACCTTTTTTCAGATAGGCCCGCACGTTGGGCGCCAGGCCGTCGTCGAACGGCGTCTGGACGATGCCGCCGTGCATGTAGAGCACGTCTTCGCGTTGGCTCGGCAGGTCTTGATGGACGTTCAGCGGCACGGCGATATCGCAGATCACGCTGCGCTCGGCGAAATGTTCGGCACCGAGGAACGGCTGAGGCGCATTGGCAGCGCACAGGACAATCCGTGCGCCCTTGATGGCGTCGAGATCGTTG  >  CP009974/5417797‑5418092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑