Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,523,283 A→G S74P (TCG→CCG)  RPPX_24660 ← hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,523,2830AG98.8% 865.0 / ‑0.7 248S74P (TCG→CCG) RPPX_24660hydratase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (121/123);  total (124/124)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 248 total aligned reads displayed.

GGTACGCGCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGACAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTCCGTCAG  >  CP009974/5523135‑5523430
                                                                                                                                                    |                                                                                                                                                   
ggTACGCGCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGCTTGTCACCCGCGCAACCGCTCGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCACGGGCCCCGCCa                                                                                                                                                   >  2:4918367/1‑151 (MQ=255)
 gTACGCGCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCaa                                                                                                                                                  >  1:3710736/1‑151 (MQ=255)
 gTACGCGCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGACaa                                                                                                                                                  >  2:212697/1‑151 (MQ=255)
   aCGCGCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACt                                                                                                                                                <  1:3362044/151‑1 (MQ=255)
    cgcgCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACtt                                                                                                                                               <  2:4716471/151‑1 (MQ=255)
      cgcCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGc                                                                                                                                             <  2:1744998/151‑1 (MQ=255)
       gcCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGcc                                                                                                                                            <  1:551742/151‑1 (MQ=255)
            gCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCAc                                                                                                                                       >  1:4022628/1‑151 (MQ=255)
             cTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCAcc                                                                                                                                      >  2:880097/1‑150 (MQ=255)
                gCGGGGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCAt                                                                                                                                   <  2:2594137/151‑1 (MQ=255)
                gCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCAt                                                                                                                                   >  1:3834597/1‑151 (MQ=255)
                  ggaggaATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGc                                                                                                                                 >  1:2219157/1‑151 (MQ=255)
                    aggaATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCa                                                                                                                               <  1:285153/151‑1 (MQ=255)
                     ggaATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAg                                                                                                                              >  2:4598604/1‑151 (MQ=255)
                      gaATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGc                                                                                                                             >  2:2016508/1‑151 (MQ=255)
                      gaATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGc                                                                                                                             <  1:504030/151‑1 (MQ=255)
                             cgcgacaaGCACGAGTGCAATTTCGCACCCGCTGAGCCCCGGCTCACTCGCTGGCAGCTTATTATGTGCGCCCACGGCATGGCTACGCCCCGTTTCCGGGTGGCCGATCGAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTg                                                                                                                      <  2:3065484/144‑1 (MQ=255)
                             ccGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTg                                                                                                                      <  1:5110742/151‑1 (MQ=255)
                               ggTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGcc                                                                                                                    >  1:3522571/1‑151 (MQ=255)
                               ggTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGcc                                                                                                                    >  1:2332438/1‑151 (MQ=255)
                                gTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCg                                                                                                                   >  1:2521875/1‑151 (MQ=255)
                                 tCCAGCACGAAGGGGATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCACCGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGa                                                                                                                  <  2:1155508/151‑1 (MQ=255)
                                    aGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGc                                                                                                               <  2:600578/151‑1 (MQ=255)
                                    aGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCCAccc                                                                                                               >  2:3832467/1‑149 (MQ=255)
                                      cACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGccc                                                                                                             >  1:2873072/1‑151 (MQ=255)
                                      cACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGccc                                                                                                             >  1:4534836/1‑151 (MQ=255)
                                       aCGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCa                                                                                                            >  1:4093174/1‑151 (MQ=255)
                                       aCGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGAGATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCa                                                                                                            <  1:3627386/151‑1 (MQ=255)
                                           aGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCtt                                                                                                        <  1:1726729/151‑1 (MQ=255)
                                           aGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCtt                                                                                                        >  1:1507341/1‑151 (MQ=255)
                                             gCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTcc                                                                                                      >  1:4267720/1‑151 (MQ=255)
                                             gCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTcc                                                                                                      >  1:1028345/1‑151 (MQ=255)
                                              cAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCa                                                                                                     <  2:2278238/151‑1 (MQ=255)
                                               aaTTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCAc                                                                                                    >  1:2749466/1‑151 (MQ=255)
                                                 tttCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCAccc                                                                                                  <  1:3242310/151‑1 (MQ=255)
                                                  ttCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCg                                                                                                 >  1:5174255/1‑151 (MQ=255)
                                                  ttCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCg                                                                                                 <  2:3670965/151‑1 (MQ=255)
                                                  ttCGCATGCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTGCCACCCg                                                                                                 <  2:4333952/151‑1 (MQ=255)
                                                    cGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgc                                                                                               <  1:705796/151‑1 (MQ=255)
                                                    cGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgc                                                                                               <  1:5220243/151‑1 (MQ=255)
                                                    cGCATGCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgc                                                                                               <  2:442399/151‑1 (MQ=255)
                                                      cATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgcgc                                                                                             <  2:3490443/151‑1 (MQ=255)
                                                      cATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgcgc                                                                                             >  1:685928/1‑151 (MQ=255)
                                                       aTTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgcgcg                                                                                            <  1:3174182/151‑1 (MQ=255)
                                                        ttCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGgcgcgc                                                                                           >  2:2049077/1‑151 (MQ=255)
                                                            aTGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGc                                                                                       >  2:266014/1‑151 (MQ=255)
                                                             gggTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGcc                                                                                      <  2:283296/150‑1 (MQ=255)
                                                                tCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGc                                                                                   >  1:4787549/1‑151 (MQ=255)
                                                                 cACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCg                                                                                  >  2:2656145/1‑151 (MQ=255)
                                                                  aCCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCCAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCgg                                                                                 <  2:4093174/151‑1 (MQ=255)
                                                                  aCCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCgg                                                                                 >  2:3610335/1‑151 (MQ=255)
                                                                    cGGCGTAACCGCTGGCCGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCAGCCCCGCTGGCCTGCGgca                                                                               >  2:1355073/1‑151 (MQ=255)
                                                                    cGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGgca                                                                               >  2:4150614/1‑151 (MQ=255)
                                                                       cGTCACCGGTTGCAGCTGGATATGTAGTCCAGCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCAGGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGgcagca                                                                            <  2:2981245/151‑1 (MQ=255)
                                                                         tAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGgcagcagc                                                                          >  1:2020700/1‑151 (MQ=255)
                                                                         tAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGgcagcagc                                                                          >  1:1260991/1‑151 (MQ=255)
                                                                         tAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGgcagcagc                                                                          <  2:474150/151‑1 (MQ=255)
                                                                          aaCCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGgcagcagca                                                                         >  1:3517104/1‑151 (MQ=255)
                                                                            ccGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCaaa                                                                       >  1:1443711/1‑151 (MQ=255)
                                                                             cGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTACCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAaac                                                                      <  1:1357032/151‑1 (MQ=255)
                                                                              gCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAaaca                                                                     >  1:2015796/1‑151 (MQ=255)
                                                                               cTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAaacaa                                                                    >  1:2147445/1‑151 (MQ=255)
                                                                                gggCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAaacaac                                                                   <  1:4936300/150‑1 (MQ=255)
                                                                                  gCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCCGCAAACAACTg                                                                 >  2:2383119/1‑151 (MQ=255)
                                                                                        tGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTg                                                           <  2:1418097/151‑1 (MQ=255)
                                                                                        tGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTg                                                           <  1:3865519/151‑1 (MQ=255)
                                                                                            atGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTg                                                       <  1:497827/151‑1 (MQ=255)
                                                                                            atGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTg                                                       >  1:4709041/1‑151 (MQ=255)
                                                                                             tgtgCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGt                                                      >  1:1198122/1‑151 (MQ=255)
                                                                                              gtgCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTc                                                     >  2:697920/1‑151 (MQ=255)
                                                                                               tgCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCa                                                    <  1:1827358/151‑1 (MQ=255)
                                                                                                gcgcCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCAt                                                   <  2:3423704/151‑1 (MQ=255)
                                                                                                 cgcCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATa                                                  <  1:3098738/151‑1 (MQ=255)
                                                                                                   ccATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGc                                                <  2:1709911/151‑1 (MQ=255)
                                                                                                   ccATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGGCATAGc                                                >  1:4884330/1‑151 (MQ=255)
                                                                                                      tCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCtt                                             <  2:1144172/151‑1 (MQ=255)
                                                                                                        ggCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGt                                           >  1:1748123/1‑151 (MQ=255)
                                                                                                         gCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCATTGtt                                          <  1:4316606/151‑1 (MQ=255)
                                                                                                          cATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGttt                                         <  1:3415255/151‑1 (MQ=255)
                                                                                                          cATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGttt                                         <  1:4161087/151‑1 (MQ=255)
                                                                                                          cATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGACAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGttt                                         >  2:3998352/1‑151 (MQ=255)
                                                                                                           aTGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTc                                        >  2:895777/1‑151 (MQ=255)
                                                                                                             ggCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCaa                                      <  2:2606642/151‑1 (MQ=255)
                                                                                                             ggCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCaa                                      <  2:4500399/151‑1 (MQ=255)
                                                                                                               cAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACCACTGGTCCAGGCTGTCATAGCCCTGTTTcgccg                                    >  2:4464145/1‑147 (MQ=255)
                                                                                                               cAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACg                                    <  1:3065340/151‑1 (MQ=255)
                                                                                                               cAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACg                                    <  2:5030254/151‑1 (MQ=255)
                                                                                                                   gCCCCGCTTCCACTTGGCCAAGCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGtctc                                <  2:2701918/151‑1 (MQ=255)
                                                                                                                     cccGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCgg                              <  1:2683508/151‑1 (MQ=255)
                                                                                                                     cccGCTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCgg                              >  1:3461228/1‑151 (MQ=255)
                                                                                                                         cTTCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCtt                          >  2:2447899/1‑151 (MQ=255)
                                                                                                                           tCCAGTTGGCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGa                        >  1:461159/1‑151 (MQ=255)
                                                                                                                                  ggCCAATCAAGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGc                 <  1:964011/151‑1 (MQ=255)
                                                                                                                                          aaGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGtt         <  1:5202463/151‑1 (MQ=255)
                                                                                                                                           aGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTc        <  2:4663498/151‑1 (MQ=255)
                                                                                                                                           aGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTc        <  1:1919533/151‑1 (MQ=255)
                                                                                                                                           aGGGCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTc        >  1:993768/1‑151 (MQ=255)
                                                                                                                                            gggCCGCGGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTcc       <  1:1601334/151‑1 (MQ=255)
                                                                                                                                                cgcgGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTCCGTca   <  1:4151909/151‑1 (MQ=255)
                                                                                                                                                 gcgGCAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTCCGTcag  <  2:2857371/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTACGCGCCCGGCTTGCGGAGGAATATCCCGGTCCAGCACGAAGGCAATTTCGCATTCGATGGTCACCGGCGTAACCGCTGGCAGCTTGATATGTGCGCCATCGGCATGGCAACGCCCCGCTTCCAGTTGGCCAATCAAGGGCCGCGACAACTTGCCTGCACGCATGCCAGCTGGGCTGCCGACGCCCAGCTTCCACCCGGCGCGCTGGCCTGCGGCAGCAGCAAACAACTGGTCCTGGCTGTCATAGCCTTGTTTCAACGTCTCGGGCTTGAGGCTGGCGGGCAGTTCCGTCAG  >  CP009974/5523135‑5523430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑