Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,587,720 A→G E294E (GAA→GAG)  RPPX_24875 → type VI secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,587,7200AG100.0% 788.6 / NA 223E294E (GAA→GAG) RPPX_24875type VI secretion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (112/111);  total (112/111)

Only 100 of 223 total aligned reads displayed.

CGCGCACTCGCAGCAGCAAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAACTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGCACAGTCACC  >  CP009974/5587572‑5587866
                                                                                                                                                    |                                                                                                                                                  
   gcACTCGCAGCAGCAAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTCGAGCTgcg                                                                                                                                               >  2:4479441/1‑151 (MQ=255)
     aCTCGCAGCAGCAAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGcc                                                                                                                                             <  1:4989500/151‑1 (MQ=255)
         gcagcagcaAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCaa                                                                                                                                         >  1:448700/1‑151 (MQ=255)
         gcagcagcaAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCaa                                                                                                                                         >  1:2149573/1‑151 (MQ=255)
           agcagcaAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCaagc                                                                                                                                       >  2:4393470/1‑151 (MQ=255)
            gcagcaAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCaagca                                                                                                                                      >  2:1719903/1‑151 (MQ=255)
              agcaAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCaagcaaa                                                                                                                                    >  1:3557429/1‑151 (MQ=255)
                 aaGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCaagcaaagca                                                                                                                                 >  1:2421989/1‑151 (MQ=255)
                   gTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACg                                                                                                                               <  1:964131/151‑1 (MQ=255)
                     tCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGTAAAGCACttt                                                                                                                             <  2:2092157/151‑1 (MQ=255)
                      cACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTg                                                                                                                            <  1:3469294/151‑1 (MQ=255)
                        ccATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTc                                                                                                                          >  1:3763532/1‑151 (MQ=255)
                         cATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTcc                                                                                                                         >  2:1014906/1‑150 (MQ=255)
                          aTCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCAc                                                                                                                        >  2:1838453/1‑151 (MQ=255)
                           tCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCAcc                                                                                                                       <  2:1320849/151‑1 (MQ=255)
                            cGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTCCGCCTCAAGCAAAGCACGTTCTCAccc                                                                                                                      >  2:4161226/1‑151 (MQ=255)
                              tGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCtt                                                                                                                    <  2:2175219/151‑1 (MQ=255)
                                aTCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCt                                                                                                                  <  2:3283293/151‑1 (MQ=255)
                                  cAGAGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGc                                                                                                                <  1:2564707/151‑1 (MQ=255)
                                   aGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCt                                                                                                               <  1:4318325/151‑1 (MQ=255)
                                    gCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCtt                                                                                                              >  1:4676534/1‑151 (MQ=255)
                                       aaCTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGcc                                                                                                           >  2:2641045/1‑150 (MQ=255)
                                       aaCTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCa                                                                                                           <  1:4150545/151‑1 (MQ=255)
                                       aaCTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCa                                                                                                           <  1:3511153/151‑1 (MQ=255)
                                         cTCGACCGGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCAcc                                                                                                         <  2:3212018/151‑1 (MQ=255)
                                         cTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCAcc                                                                                                         <  1:3687971/151‑1 (MQ=255)
                                           cGACCCGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGc                                                                                                       <  1:325228/151‑1 (MQ=255)
                                           cGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGc                                                                                                       <  2:135988/151‑1 (MQ=255)
                                            gACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCa                                                                                                      >  2:4305440/1‑151 (MQ=255)
                                             aCCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAg                                                                                                     <  1:1503367/151‑1 (MQ=255)
                                                aGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCa                                                                                                  <  1:4872383/151‑1 (MQ=255)
                                                aGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCa                                                                                                  <  1:3041530/151‑1 (MQ=255)
                                                 ggcGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAg                                                                                                 <  2:338977/149‑1 (MQ=255)
                                                    gCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGcc                                                                                              <  2:1333284/151‑1 (MQ=255)
                                                      aGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATTACCTGGAGCTGCGTCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGc                                                                                            >  2:5015949/1‑151 (MQ=255)
                                                       gCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCa                                                                                           <  2:1985962/151‑1 (MQ=255)
                                                        cTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCAt                                                                                          <  2:3459557/151‑1 (MQ=255)
                                                         tGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATa                                                                                         <  2:4735148/151‑1 (MQ=255)
                                                         tGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATa                                                                                         >  1:783124/1‑151 (MQ=255)
                                                           cGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATAcc                                                                                       >  1:2977595/1‑151 (MQ=255)
                                                            gggACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCt                                                                                      >  1:3752378/1‑151 (MQ=255)
                                                            gggACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCt                                                                                      <  2:608357/151‑1 (MQ=255)
                                                                 ttGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCt                                                                                 >  2:2050577/1‑151 (MQ=255)
                                                                       cccAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAg                                                                                                 >  2:1086407/1‑129 (MQ=255)
                                                                       cccAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCa                                                                           <  1:2842105/151‑1 (MQ=255)
                                                                        ccAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAg                                                                          >  2:2223763/1‑151 (MQ=255)
                                                                        ccAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAg                                                                          >  1:1093328/1‑151 (MQ=255)
                                                                          ggCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCt                                                                        <  2:1403824/150‑1 (MQ=255)
                                                                          aGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCt                                                                        <  1:694903/151‑1 (MQ=255)
                                                                           gCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCtt                                                                       <  1:1865628/151‑1 (MQ=255)
                                                                           gCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCtt                                                                       <  1:1351206/151‑1 (MQ=255)
                                                                             ggCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTtcg                                                                     >  1:1643902/1‑151 (MQ=255)
                                                                              gCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTtcgt                                                                    >  2:760510/1‑151 (MQ=255)
                                                                              gCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTCGCACCGCCGCCAGGCCGCATACCTCGGCTGGACCAGCTccgc                                                                    >  2:4058803/1‑150 (MQ=255)
                                                                               cATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTtcgtc                                                                   <  2:1505971/151‑1 (MQ=255)
                                                                                  aaaCTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTcgac                                                                <  1:305472/150‑1 (MQ=255)
                                                                                    aCTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCCGCGCCTCAAGCAAAGCACGTTGTCCCCCTTCTCCCTGCACCCCACCCAGGCCGCACACCTGGGCCGGCCCCGCTTCGccgccgc                                                              >  2:4261274/1‑150 (MQ=255)
                                                                                        cGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCgg                                                          <  2:4141716/151‑1 (MQ=255)
                                                                                        cGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCgg                                                          <  2:882981/151‑1 (MQ=255)
                                                                                         ggCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGc                                                         <  2:3868410/151‑1 (MQ=255)
                                                                                          gCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCa                                                        <  2:3736761/151‑1 (MQ=255)
                                                                                          gCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCa                                                        >  1:2509598/1‑151 (MQ=255)
                                                                                            cGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATg                                                      <  1:2833978/151‑1 (MQ=255)
                                                                                            cGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATg                                                      >  1:3902980/1‑151 (MQ=255)
                                                                                              tttGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGc                                                    <  1:4207871/151‑1 (MQ=255)
                                                                                               ttGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGcc                                                   <  1:5172277/151‑1 (MQ=255)
                                                                                                tGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCa                                                  <  2:1899535/151‑1 (MQ=255)
                                                                                                    tGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATc                                              <  1:3110401/151‑1 (MQ=255)
                                                                                                       aCTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGc                                           <  1:1005243/151‑1 (MQ=255)
                                                                                                       aCTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGc                                           >  1:3815578/1‑151 (MQ=255)
                                                                                                          gATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGACCAGCCCg                                        >  2:2499328/1‑151 (MQ=255)
                                                                                                           cgcGACTTTCTGCGGGGTGAGGGGCGGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCgg                                       <  2:5154206/149‑1 (MQ=255)
                                                                                                            tCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGt                                      <  2:3902980/151‑1 (MQ=255)
                                                                                                              gACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTg                                    >  1:3358451/1‑151 (MQ=255)
                                                                                                               aCTTTCTGCTGCGTGACGGGCTGGCCTATTACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTCCGTCGACGACCGGCACGGCCAGATCAGCCCGGTTGt                                   >  2:2965379/1‑151 (MQ=255)
                                                                                                               aCTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGt                                   <  1:3446902/151‑1 (MQ=255)
                                                                                                                cTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTa                                  >  1:1187722/1‑151 (MQ=255)
                                                                                                                 tttCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTAc                                 <  2:2414103/151‑1 (MQ=255)
                                                                                                                   tCTGCTGCGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACgg                               >  2:549765/1‑150 (MQ=255)
                                                                                                                     tgctgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTAt                             >  2:4827855/1‑151 (MQ=255)
                                                                                                                     tgctgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTAt                             <  2:1657634/151‑1 (MQ=255)
                                                                                                                     tgctgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTAt                             >  1:4273281/1‑151 (MQ=255)
                                                                                                                      gctgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATc                            <  1:2653716/151‑1 (MQ=255)
                                                                                                                       ctgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATcc                           <  1:1034984/151‑1 (MQ=255)
                                                                                                                       ctgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATcc                           >  2:2781977/1‑151 (MQ=255)
                                                                                                                        tgcGTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCg                          <  2:3700751/151‑1 (MQ=255)
                                                                                                                           gTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCgggg                       >  1:328947/1‑151 (MQ=255)
                                                                                                                           gTGACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCgggg                       >  1:4566830/1‑151 (MQ=255)
                                                                                                                             gACGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGgc                     >  1:5224068/1‑151 (MQ=255)
                                                                                                                              aCGGGCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGgcg                    <  1:1753080/151‑1 (MQ=255)
                                                                                                                                gggCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGca                  >  1:1467222/1‑151 (MQ=255)
                                                                                                                                  gCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGcaca                >  1:1373018/1‑151 (MQ=255)
                                                                                                                                  gCTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGcaca                <  2:315334/151‑1 (MQ=255)
                                                                                                                                   cTGGCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACAt               <  2:1221957/151‑1 (MQ=255)
                                                                                                                                     ggCCTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGa             >  1:3986935/1‑151 (MQ=255)
                                                                                                                                        cTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGcc          >  2:1516225/1‑150 (MQ=255)
                                                                                                                                        cTATGACCTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGca          <  1:101198/151‑1 (MQ=255)
                                                                                                                                              ccTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGCACAGTCa    >  1:3771779/1‑151 (MQ=255)
                                                                                                                                               cTGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGCACAGTCAc   >  2:580911/1‑151 (MQ=255)
                                                                                                                                                tGGAGCTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGCACAGTCAcc  >  2:3807556/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCGCACTCGCAGCAGCAAGTTCACCATCGTGATCAGCGAACTCGACCAGACGCAGCTGCGGGACTTGCTGCCCAGCGGCATCAACTTCGGCCGTTTGCGTGCACTGATCGACTTTCTGCTGCGTGACGGGCTGGCCTATGACCTGGAACTGCGCCTCAAGCAAAGCACGTTGTCACCCTTCTGCTTGCACCGCAGCCAGGCCGCATACCTGGGCTGGACCAGCTTCGTCGACGACCGGCATGGCCAGATCAGCCCGGTTGTACGTATCCGGGGGCGCACATGAGCACAGTCACC  >  CP009974/5587572‑5587866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑