Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,603,289 G→A E339K (GAA→AAA)  RPPX_24935 → isoquinoline 1‑oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,603,2890GA99.2% 911.7 / ‑5.3 244E339K (GAA→AAA) RPPX_24935isoquinoline 1‑oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (113/128);  total (114/130)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

TGGTGGCACGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATGAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTCGACGTC  >  CP009974/5603142‑5603435
                                                                                                                                                   |                                                                                                                                                  
tggtggCACGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAag                                                                                                                                                 <  1:3400370/151‑1 (MQ=255)
tggtggCACGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAag                                                                                                                                                 <  2:684882/151‑1 (MQ=255)
 ggtggCACGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAaga                                                                                                                                                <  1:2173652/151‑1 (MQ=255)
    ggCACGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGaa                                                                                                                                             <  2:4754292/151‑1 (MQ=255)
        cGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGaa                                                                                                                                         >  2:342479/1‑151 (MQ=255)
            aggCGTGCAGGGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAgggg                                                                                                                                     <  2:4285529/149‑1 (MQ=255)
               cGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGata                                                                                                                                  <  2:3317135/151‑1 (MQ=255)
               cGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGata                                                                                                                                  <  1:3963193/151‑1 (MQ=255)
               cGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGata                                                                                                                                  >  1:2206885/1‑151 (MQ=255)
                 tGCAGTGGAGGCGATGCAGGTAGACTGGAGAGAGCCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATAtt                                                                                                                                <  1:653725/151‑1 (MQ=255)
                     gTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCg                                                                                                                            >  1:4469930/1‑151 (MQ=255)
                     gTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCg                                                                                                                            <  2:1937029/151‑1 (MQ=255)
                      tGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCgg                                                                                                                           <  1:4506512/151‑1 (MQ=255)
                      tGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCgg                                                                                                                           >  1:760805/1‑151 (MQ=255)
                       ggAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGgc                                                                                                                          >  1:4710035/1‑151 (MQ=255)
                        gAGGCGATGGAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGgcg                                                                                                                         <  2:1477086/151‑1 (MQ=255)
                         aGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGgcgc                                                                                                                        >  2:3859187/1‑151 (MQ=255)
                          ggCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGCTATTGCCGGCGcc                                                                                                                       >  2:2836639/1‑151 (MQ=255)
                           gCGATGCAGGTCGACTGGAAAGAACCGGCGGCCGACGGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCt                                                                                                                      <  2:1348025/151‑1 (MQ=255)
                           gCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCt                                                                                                                      >  1:3721571/1‑151 (MQ=255)
                           gCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCt                                                                                                                      >  1:1143851/1‑151 (MQ=255)
                            cGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCtt                                                                                                                     <  2:4520068/151‑1 (MQ=255)
                             gATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTg                                                                                                                    >  1:3891352/1‑151 (MQ=255)
                             gATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTg                                                                                                                    >  1:3577889/1‑151 (MQ=255)
                              aTGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCTTTTTTTTCCTGGCGCGAACACCCCGCGACGGTGCAAGGCCCGTCCCGTGATAAAGAGAACGGAGGGGCTATTGCCGGCGCCTTgg                                                                                                                   >  2:809470/1‑151 (MQ=255)
                               tGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGc                                                                                                                  >  2:5206927/1‑151 (MQ=255)
                                gCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGcc                                                                                                                 >  1:4027720/1‑151 (MQ=255)
                                  aGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCaa                                                                                                               <  2:4168896/151‑1 (MQ=255)
                                  aGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCaa                                                                                                               >  1:4267717/1‑151 (MQ=255)
                                   ggTAGACTGGAAAGACCCGGCCGACGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTCCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAAc                                                                                                              <  2:68908/151‑1 (MQ=255)
                                    gTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACg                                                                                                             <  1:2964738/151‑1 (MQ=255)
                                      aGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGcc                                                                                                           >  1:4865821/1‑151 (MQ=255)
                                         cTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCaaa                                                                                                        >  2:1327133/1‑150 (MQ=255)
                                           ggAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGAc                                                                                                      <  2:3464630/151‑1 (MQ=255)
                                            gAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTTCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGAcc                                                                                                     >  2:2394822/1‑151 (MQ=255)
                                             agaGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCGGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGAccc                                                                                                    <  2:5226467/149‑1 (MQ=255)
                                              aaGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCa                                                                                                   <  1:2478877/151‑1 (MQ=255)
                                               aGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAg                                                                                                  >  2:3886890/1‑151 (MQ=255)
                                                gAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAgg                                                                                                 >  1:2015764/1‑151 (MQ=255)
                                                 aaCCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGt                                                                                                <  1:1765057/151‑1 (MQ=255)
                                                  aCCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTc                                                                                               <  1:1278514/151‑1 (MQ=255)
                                                         gcccACAGCAAAGTGCGCCAGATGCCGGCCGATTTCTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCa                                                                                        <  2:3143788/151‑1 (MQ=255)
                                                           cgACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTTCAGCGACTCCTGGCGCGAACACCTCGCGACGGTCCAAGGGCCGTCCCGTGATAAAGAGAACGAAGCGGATATTGCCGGCGCCCTGGCCCCCCCCCAGACCCGGGTCGAGCCCCGc                                                                                      >  2:3505544/1‑151 (MQ=255)
                                                            gACAGCAAAGTGCGCCCGCTGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCGCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCt                                                                                     <  2:1729608/151‑1 (MQ=255)
                                                               aGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTAcc                                                                                  <  2:1404464/151‑1 (MQ=255)
                                                                gCAACGTGCGGCCGAGGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACca                                                                                 <  2:3115393/151‑1 (MQ=255)
                                                                  aaaGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACcaca                                                                               >  2:4460303/1‑151 (MQ=255)
                                                                   acgTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACaa                                                                              <  2:1454871/149‑1 (MQ=255)
                                                                   aaGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACaa                                                                              <  1:1561763/151‑1 (MQ=255)
                                                                      ggcgcCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCa                                                                           <  2:2148043/150‑1 (MQ=255)
                                                                       gcgcCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAg                                                                          >  1:4864721/1‑151 (MQ=255)
                                                                       gcgcCAGATGCCGGCCGATTTATCCAGCGACGCCTGGCGCGAACACATCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCGCAACCAg                                                                          <  1:1005670/151‑1 (MQ=255)
                                                                          cccGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTAc                                                                       >  1:1671168/1‑151 (MQ=255)
                                                                          cccGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTAc                                                                       <  1:1980885/151‑1 (MQ=255)
                                                                           ccGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTAcc                                                                      <  1:4286292/151‑1 (MQ=255)
                                                                           ccGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTAcc                                                                      >  1:904247/1‑151 (MQ=255)
                                                                             gATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGCGGCCAGCTACCACAACCAGTACCTg                                                                    >  1:2582055/1‑151 (MQ=255)
                                                                                gccggccgATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAAc                                                                 <  1:1358896/151‑1 (MQ=255)
                                                                                  cggccgATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCa                                                               >  1:3240967/1‑151 (MQ=255)
                                                                                   ggccgATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCAt                                                              <  1:3655016/151‑1 (MQ=255)
                                                                                       gATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGccc                                                          >  1:3907555/1‑151 (MQ=255)
                                                                                       gATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGccc                                                          <  2:2194079/151‑1 (MQ=255)
                                                                                        aTTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCa                                                         >  2:468386/1‑151 (MQ=255)
                                                                                         tttttCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAg                                                        <  1:580332/151‑1 (MQ=255)
                                                                                         ttctcccAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAg                                                        <  2:2926558/146‑1 (MQ=255)
                                                                                            ttCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTg                                                     >  1:2684336/1‑151 (MQ=255)
                                                                                               cAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAg                                                  <  2:3680072/151‑1 (MQ=255)
                                                                                                aGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGc                                                 <  2:264022/151‑1 (MQ=255)
                                                                                                 gCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGcc                                                <  1:3819484/151‑1 (MQ=255)
                                                                                                 gCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGcc                                                <  1:285703/151‑1 (MQ=255)
                                                                                                  cGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCa                                               >  1:345305/1‑151 (MQ=255)
                                                                                                      gCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGt                                           >  2:1872892/1‑151 (MQ=255)
                                                                                                      gCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCAAAGACCCAGGTCGGCGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGt                                           >  2:5019016/1‑151 (MQ=255)
                                                                                                        cTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTcc                                         >  1:841917/1‑151 (MQ=255)
                                                                                                          ggCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCAccgcccgc                                       >  2:2886845/1‑151 (MQ=255)
                                                                                                           gcgcGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGcc                                      <  2:3934569/151‑1 (MQ=255)
                                                                                                             gcgAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCt                                    <  2:287724/151‑1 (MQ=255)
                                                                                                              cgAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTc                                   <  2:1155693/151‑1 (MQ=255)
                                                                                                              cgAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTc                                   >  2:4962970/1‑151 (MQ=255)
                                                                                                              cgAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTc                                   <  1:3340887/151‑1 (MQ=255)
                                                                                                              cgAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTc                                   <  1:3279078/151‑1 (MQ=255)
                                                                                                              cgAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTc                                   <  1:1183534/151‑1 (MQ=255)
                                                                                                               gAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCg                                  >  1:2795897/1‑151 (MQ=255)
                                                                                                                      tCGCGACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCtt                           <  2:904247/151‑1 (MQ=255)
                                                                                                                          gACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCATCGTCCGCCCTCGCCAGGTGCAAc                       >  2:4381874/1‑151 (MQ=255)
                                                                                                                          gACGGTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAAc                       >  1:390063/1‑151 (MQ=255)
                                                                                                                             ggTGCAAGGGCCGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCa                    <  1:5106398/151‑1 (MQ=255)
                                                                                                                                      gCCGTCCCGTGATAAGGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGc           <  1:978844/151‑1 (MQ=255)
                                                                                                                                       ccGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCCCGCCCGCCTCAACCCAGACCGCAGcc          >  2:3723189/1‑151 (MQ=255)
                                                                                                                                        cGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCt         >  1:5083704/1‑151 (MQ=255)
                                                                                                                                        cGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCt         <  1:2256520/151‑1 (MQ=255)
                                                                                                                                        cGTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCt         >  1:1035727/1‑151 (MQ=255)
                                                                                                                                         gTCCCGTTACCAAGCGAACCAAGGGGATATTGCCGGCGCCTTGGCCAACACCAAGACCCAGGCAGACACCAGCTACCACAACCACCACCTGAACCATGCCCAGCTGGAGCCACCGCCCGCTCACGGCCGCTCCCACCCCCCCGGCAGcccc        >  2:3256467/1‑149 (MQ=255)
                                                                                                                                         gTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTc        <  2:3577889/151‑1 (MQ=255)
                                                                                                                                         gTCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTc        >  1:4762823/1‑151 (MQ=255)
                                                                                                                                          tCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTCg       >  2:2420506/1‑151 (MQ=255)
                                                                                                                                          tCCCGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTCg       <  1:1843157/151‑1 (MQ=255)
                                                                                                                                             cGTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTCGACg    >  1:1014931/1‑151 (MQ=255)
                                                                                                                                              gTGATAAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTCGACGt   <  1:3422768/151‑1 (MQ=255)
                                                                                                                                               tgataaAGAGAACGAAGGGGATATTGCCGGCGCCTTCGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGGCCTCGCCCGCTTCAACCCAGACGGCAGCCTCGACGTc  >  2:4106487/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGTGGCACGCCAAGCGTGCAGTGGAGGCGATGCAGGTAGACTGGAAAGAACCGGCCGCCGACAGCAAAGTGCGCCCGATGCCGGCCGATTTTTCCAGCGACGCCTGGCGCGAACACCTCGCGACGGTGCAAGGGCCGTCCCGTGATGAAGAGAACGAAGGGGATATTGCCGGCGCCTTGGCCAACGCCAAGACCCAGGTCGAGGCCAGCTACCACAACCAGTACCTGAACCATGCCCAGCTGGAGCCACCGTCCGCCCTCGCCCGCTTCAACCCAGACGGCAGCCTCGACGTC  >  CP009974/5603142‑5603435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑