Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	768,193	A→G	G462G (GGT→GGC)	RPPX_03350 ←	acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	768,193	0	A	G	78.6%	10.5 / ‑1.4	14	G462G (GGT→GGC)	RPPX_03350	acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (7/4);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTGCCGTAGTCCAGGCCGAAATCGGTGAAGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAACCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAT  >  CP009974/768126‑768340                                                                    |                                                                                                                                                    gggtGCCGTAGTCCAGGCCGAAATCGCTGACGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAACCGGCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAgcc                                                                  <  2:4217551/148‑1 (MQ=0)                          ggggAAGCCCATGTTGGCCTGCTTCAAGCGGATCATGCCGTAACCGTCGTGACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGATGCAGGCGTCGGCACCCACCGAGATTAcc                                         <  2:3425174/148‑1 (MQ=1)                                                           cATGCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCAtt        >  1:2863822/1‑151 (MQ=11)                                                           cATGCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCAtt        <  1:840458/151‑1 (MQ=11)                                                              gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCGGCCATTGcc     <  2:2626590/151‑1 (MQ=14)                                                              gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGGCCGGGTGCACCAGGTGCGCAGCCATTGcc     <  2:3081606/151‑1 (MQ=14)                                                               ccGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCGCCAGGTGCGCAGCCATTTCCg    >  2:4286783/1‑151 (MQ=9)                                                               ccGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCg    >  2:1235011/1‑151 (MQ=14)                                                                cGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGa   <  1:830711/151‑1 (MQ=17)                                                                cGTAACCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGa   >  2:4558621/1‑151 (MQ=31)                                                                 gTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAt  >  1:115312/1‑151 (MQ=17)                                                                 gTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAt  >  1:1948757/1‑151 (MQ=17)                                                                 gTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAt  >  2:893401/1‑151 (MQ=17)                                                                 gTAGCCGTCGTCACGCAGGATCACCACGGGGATGTGCATGCCCAGACGTACTGCCGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGGCCGGGTGCACCCGGGGCGCAGCCATTGCCGag  >  2:3372384/1‑150 (MQ=11)                                                                    |                                                                                                                                                    GGTTGCCGTAGTCCAGGCCGAAATCGGTGAAGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAACCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAT  >  CP009974/768126‑768340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑