Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	775,414	C→T	intergenic (‑176/‑282)	RPPX_03375 ← / → RPPX_03380	16S ribosomal RNA/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	775,414	0	C	T	100.0%	293.2 / NA	94	intergenic (‑176/‑282)	RPPX_03375/RPPX_03380	16S ribosomal RNA/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/44);  total (50/44)

GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAG  >  CP009974/775347‑775560                                                                    |                                                                                                                                                   gTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttc                                                                 <  1:1523598/151‑1 (MQ=1)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                              <  2:1000449/151‑1 (MQ=255)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                              <  1:4941681/151‑1 (MQ=11)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                              >  1:4380531/1‑151 (MQ=255)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                             >  1:2988714/1‑151 (MQ=11)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                             >  1:310789/1‑151 (MQ=11)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                             >  1:774451/1‑151 (MQ=255)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                             <  1:2076717/151‑1 (MQ=255)      tACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCg                                                            <  1:4713566/151‑1 (MQ=11)       aCTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGa                                                           >  2:2550350/1‑151 (MQ=255)        cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                          <  1:1713395/151‑1 (MQ=11)         tCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaaa                                                         >  2:3616535/1‑151 (MQ=255)          cacaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAg                                                        >  2:4285498/1‑151 (MQ=255)           acaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGc                                                       >  2:3275291/1‑151 (MQ=255)           acaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGc                                                       >  2:4433727/1‑151 (MQ=255)           acaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGc                                                       >  2:1848071/1‑151 (MQ=255)            caAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCt                                                      <  1:266788/151‑1 (MQ=11)             aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                     <  1:4274688/151‑1 (MQ=14)                cACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaaca                                                  <  1:4685276/151‑1 (MQ=14)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGACAGCTaacaa                                                 >  2:3109901/1‑151 (MQ=14)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                 <  1:1242193/151‑1 (MQ=255)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                 >  1:914648/1‑151 (MQ=14)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                 >  1:913704/1‑151 (MQ=255)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                 <  1:2404823/151‑1 (MQ=255)                   ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                               >  1:5103402/1‑151 (MQ=255)                   ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                               >  2:2006251/1‑151 (MQ=255)                   ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                               <  1:3449752/151‑1 (MQ=255)                     acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                             <  2:4584486/151‑1 (MQ=255)                     acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                             >  2:3777594/1‑151 (MQ=255)                       acGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaa                                           <  2:1302437/151‑1 (MQ=255)                       acGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaa                                           >  1:545073/1‑151 (MQ=255)                       acGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaa                                           >  1:545054/1‑151 (MQ=255)                          aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                        <  1:2571221/151‑1 (MQ=255)                          aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                        <  2:1416018/151‑1 (MQ=17)                          aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                        >  1:1820984/1‑151 (MQ=255)                           aTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCaacaac                                       >  2:4311293/1‑151 (MQ=255)                           aTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCaacaac                                       >  1:4029576/1‑151 (MQ=17)                            ttGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACt                                      >  1:701829/1‑151 (MQ=255)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                    >  1:4678390/1‑151 (MQ=17)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                    >  1:4680173/1‑151 (MQ=17)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                    <  1:2491472/151‑1 (MQ=17)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGGTTATTTTGAAGTTTTTTACCACCACTTTCTTTCCAAAGCTAACAACTTCAACAACTTa                                    >  2:1240632/1‑151 (MQ=17)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTCAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                    >  2:1240698/1‑151 (MQ=17)                               cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTGTTTTACCACCACTCTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                   >  2:895785/1‑151 (MQ=17)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                  >  2:2205233/1‑151 (MQ=17)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                  >  2:108787/1‑151 (MQ=255)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                  >  2:2151243/1‑151 (MQ=17)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                  <  2:5225814/151‑1 (MQ=255)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                  <  2:4047199/151‑1 (MQ=255)                                 tGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCg                                 <  2:4302456/151‑1 (MQ=255)                                  gATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGc                                >  1:1486650/1‑151 (MQ=255)                                   aTTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCt                               <  2:465825/151‑1 (MQ=17)                                    ttCGATTTGATAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCtt                              <  2:2694943/151‑1 (MQ=18)                                     tCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTg                             <  2:227881/151‑1 (MQ=255)                                       gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                           <  1:2251279/151‑1 (MQ=18)                                       gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                           <  2:1454573/151‑1 (MQ=255)                                       gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                           <  2:5179282/151‑1 (MQ=255)                                        aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAGGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTGTCGAAAGCTAACAACTTCAACAACTTAGCGCTTGccc                          >  2:4108566/1‑151 (MQ=17)                                        aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                          <  1:1511929/151‑1 (MQ=18)                                        aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                          >  1:259468/1‑151 (MQ=255)                                         tttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcccc                         >  1:2614853/1‑151 (MQ=18)                                          ttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCg                        <  2:3039181/151‑1 (MQ=18)                                           tGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                       <  2:4413888/151‑1 (MQ=255)                                            gTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGaa                      <  2:2262108/151‑1 (MQ=18)                                            gTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGaa                      >  2:2909839/1‑151 (MQ=18)                                              tAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGc                    <  2:4781747/151‑1 (MQ=255)                                               aaaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCg                   >  2:2556896/1‑151 (MQ=255)                                                aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                  >  2:1754080/1‑151 (MQ=21)                                                aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                  >  1:1823695/1‑151 (MQ=21)                                                aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                  <  2:248501/151‑1 (MQ=255)                                                aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                  <  1:2534136/151‑1 (MQ=255)                                                  gagCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTc                <  1:2188833/151‑1 (MQ=255)                                                   agCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCa               <  1:2679972/151‑1 (MQ=255)                                                   agCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCa               <  2:3581675/151‑1 (MQ=255)                                                    gCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTTCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTATCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGGTCAt              >  1:14311/1‑151 (MQ=21)                                                    gCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCAt              <  1:660267/151‑1 (MQ=21)                                                     cGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACTACCACTTTCTTTCGAAAGCTTACAACTCCAACAACTTACCGCTTGCCCCGCAGCGTTCacc             >  2:2679960/1‑149 (MQ=18)                                                     cGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATc             <  1:732573/151‑1 (MQ=255)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg            <  1:1635445/151‑1 (MQ=255)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg            >  1:3100816/1‑151 (MQ=21)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg            <  1:3159799/151‑1 (MQ=21)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg            >  1:4826982/1‑151 (MQ=255)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg            >  1:420998/1‑151 (MQ=21)                                                       tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGc           >  2:2770309/1‑151 (MQ=255)                                                        ttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCt          >  2:917053/1‑151 (MQ=21)                                                         tGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTg         <  1:4460683/151‑1 (MQ=21)                                                          ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGACAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCTTTCATCGCTGc        >  2:3619680/1‑151 (MQ=21)                                                          ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGc        >  1:1665892/1‑151 (MQ=21)                                                           gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg       >  2:3105825/1‑151 (MQ=255)                                                             tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag     >  1:942572/1‑151 (MQ=25)                                                             tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag     >  2:2527549/1‑151 (MQ=25)                                                               agagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgagga   <  1:3856144/151‑1 (MQ=25)                                                                gagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggag  <  1:2558183/151‑1 (MQ=255)                                                                gagCTTTNCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggan  <  1:3099375/151‑2 (MQ=25)                                                                    |                                                                                                                                                   GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAG  >  CP009974/775347‑775560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑