Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 775,456 G→A intergenic (‑218/‑240) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974775,4560GA100.0% 502.7 / NA 152intergenic (‑218/‑240)RPPX_03375/RPPX_0338016S ribosomal RNA/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (77/75);  total (77/75)

Only 100 of 154 total aligned reads displayed.

GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCT  >  CP009974/775347‑775603
                                                                                                             |                                                                                                                                                   
gTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttc                                                                                                            <  1:1523598/151‑1 (MQ=1)
   cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                                                                         <  1:4941681/151‑1 (MQ=11)
   cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                                                                         <  2:1000449/151‑1 (MQ=255)
      aCTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGa                                                                                                      >  2:2550350/1‑151 (MQ=255)
       cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                                                                     <  1:1713395/151‑1 (MQ=11)
        tCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaaa                                                                                                    >  2:3616535/1‑151 (MQ=255)
         cacaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAg                                                                                                   >  2:4285498/1‑151 (MQ=255)
          acaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGc                                                                                                  >  2:4433727/1‑151 (MQ=255)
          acaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGc                                                                                                  >  2:1848071/1‑151 (MQ=255)
           caAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCt                                                                                                 <  1:266788/151‑1 (MQ=11)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                                                                <  1:4274688/151‑1 (MQ=14)
               cACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaaca                                                                                             <  1:4685276/151‑1 (MQ=14)
                aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGACAGCTaacaa                                                                                            >  2:3109901/1‑151 (MQ=14)
                aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                                                            >  1:913704/1‑151 (MQ=255)
                aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                                                            <  1:1242193/151‑1 (MQ=255)
                  ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                                                                          >  1:5103402/1‑151 (MQ=255)
                  ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                                                                          <  1:3449752/151‑1 (MQ=255)
                    acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                                                                        <  2:4584486/151‑1 (MQ=255)
                    acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                                                                        >  2:3777594/1‑151 (MQ=255)
                      acGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaa                                                                                      >  1:545054/1‑151 (MQ=255)
                         aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                                                                   <  2:1416018/151‑1 (MQ=17)
                         aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                                                                   <  1:2571221/151‑1 (MQ=255)
                             gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                                                               <  1:2491472/151‑1 (MQ=17)
                             gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                                                               >  1:4678390/1‑151 (MQ=17)
                             gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGGTTATTTTGAAGTTTTTTACCACCACTTTCTTTCCAAAGCTAACAACTTCAACAACTTa                                                                               >  2:1240632/1‑151 (MQ=17)
                             gCTTGATTCGATTTGTTAAAGAGCGTTTGGTCAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                                                               >  2:1240698/1‑151 (MQ=17)
                              cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTGTTTTACCACCACTCTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                                                              >  2:895785/1‑151 (MQ=17)
                               ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                                                             >  2:2151243/1‑151 (MQ=17)
                                tGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCg                                                                            <  2:4302456/151‑1 (MQ=255)
                                   ttCGATTTGATAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCtt                                                                         <  2:2694943/151‑1 (MQ=18)
                                    tCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTg                                                                        <  2:227881/151‑1 (MQ=255)
                                      gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                                                                      <  2:5179282/151‑1 (MQ=255)
                                      gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                                                                      <  2:1454573/151‑1 (MQ=255)
                                      gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                                                                      <  1:2251279/151‑1 (MQ=18)
                                       aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAGGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTGTCGAAAGCTAACAACTTCAACAACTTAGCGCTTGccc                                                                     >  2:4108566/1‑151 (MQ=17)
                                       aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                                                                     <  1:1511929/151‑1 (MQ=18)
                                       aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                                                                     >  1:259468/1‑151 (MQ=255)
                                          tGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                                                                  <  2:4413888/151‑1 (MQ=255)
                                           gTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGaa                                                                 >  2:2909839/1‑151 (MQ=18)
                                           gTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGaa                                                                 <  2:2262108/151‑1 (MQ=18)
                                             tAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGc                                                               <  2:4781747/151‑1 (MQ=255)
                                               aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                                                             >  2:1754080/1‑151 (MQ=21)
                                               aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                                                             <  1:2534136/151‑1 (MQ=255)
                                               aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                                                             >  1:1823695/1‑151 (MQ=21)
                                                 gagCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTc                                                           <  1:2188833/151‑1 (MQ=255)
                                                  agCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCa                                                          <  1:2679972/151‑1 (MQ=255)
                                                  agCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCa                                                          <  2:3581675/151‑1 (MQ=255)
                                                   gCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTTCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTATCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGGTCAt                                                         >  1:14311/1‑151 (MQ=21)
                                                   gCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCAt                                                         <  1:660267/151‑1 (MQ=21)
                                                    cGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACTACCACTTTCTTTCGAAAGCTTACAACTCCAACAACTTACCGCTTGCCCCGCAGCGTTCacc                                                        >  2:2679960/1‑149 (MQ=18)
                                                    cGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATc                                                        <  1:732573/151‑1 (MQ=255)
                                                     gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg                                                       >  1:4826982/1‑151 (MQ=255)
                                                     gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg                                                       <  1:3159799/151‑1 (MQ=21)
                                                     gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg                                                       >  1:3100816/1‑151 (MQ=21)
                                                      tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGc                                                      >  2:2770309/1‑151 (MQ=255)
                                                        tGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTg                                                    <  1:4460683/151‑1 (MQ=21)
                                                         ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGc                                                   >  1:1665892/1‑151 (MQ=21)
                                                          gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg                                                  >  2:3105825/1‑151 (MQ=255)
                                                            tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag                                                >  2:2527549/1‑151 (MQ=25)
                                                            tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag                                                >  1:942572/1‑151 (MQ=25)
                                                              agagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgagga                                              <  1:3856144/151‑1 (MQ=25)
                                                               gagCTTTNCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggan                                             <  1:3099375/151‑2 (MQ=25)
                                                                  cttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCg                                          <  2:3031958/149‑1 (MQ=255)
                                                                   ttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGa                                         <  2:1774206/150‑1 (MQ=255)
                                                                   ttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGa                                         >  1:171701/2‑151 (MQ=255)
                                                                     ttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaat                                       <  2:4168678/151‑1 (MQ=255)
                                                                      tCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaata                                      >  2:4137607/1‑151 (MQ=255)
                                                                      tCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaata                                      <  1:2110686/151‑1 (MQ=255)
                                                                      tCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaata                                      >  2:724324/1‑151 (MQ=255)
                                                                      tCGTCTCAACCGACGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaata                                      <  1:5038735/151‑1 (MQ=255)
                                                                         tctcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAataata                                   >  1:2656243/1‑151 (MQ=255)
                                                                           tcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcg                                 <  1:1430488/151‑1 (MQ=255)
                                                                           tcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcg                                 <  1:1093331/151‑1 (MQ=255)
                                                                            cAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCACTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcaa                                >  1:2859046/1‑149 (MQ=255)
                                                                             aaCCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTGCATCGCTGCGAGGAGGCGAATAATAcgcg                               >  1:1122924/1‑151 (MQ=255)
                                                                              aCCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACgcgc                              >  1:3240118/1‑151 (MQ=255)
                                                                                cGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCtt                            <  1:708006/151‑1 (MQ=255)
                                                                                    gcgcgcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGaa                        <  2:2193434/151‑1 (MQ=255)
                                                                                     cgcgcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGaaa                       <  1:1807725/151‑1 (MQ=255)
                                                                                      gcgcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAAt                      >  2:3227188/1‑151 (MQ=255)
                                                                                        gcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCa                    >  1:4231674/1‑151 (MQ=255)
                                                                                        gcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCa                    >  1:657013/1‑151 (MQ=255)
                                                                                         cATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCag                   <  2:3776728/151‑1 (MQ=255)
                                                                                          aTTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCaga                  >  1:1259971/1‑151 (MQ=255)
                                                                                          aTTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCaga                  <  2:1618799/151‑1 (MQ=255)
                                                                                             cTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTc               >  2:4535134/1‑151 (MQ=255)
                                                                                               aCGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCaa             <  1:1956553/151‑1 (MQ=255)
                                                                                                 gCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAaca           <  1:2045939/151‑1 (MQ=255)
                                                                                                 gCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAaca           >  1:2867871/1‑151 (MQ=255)
                                                                                                    ttCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAACGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACCCCCTTTCAAATCAGAGTCAACACCt        >  2:3064928/1‑151 (MQ=39)
                                                                                                     tCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcg                                 <  1:794622/125‑1 (MQ=255)
                                                                                                     tCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCtt       <  1:4705037/151‑1 (MQ=255)
                                                                                                     tCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCtt       <  1:3291264/151‑1 (MQ=255)
                                                                                                      ccTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACATTg      >  1:3773311/1‑151 (MQ=255)
                                                                                                       cTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGt     <  2:673077/151‑1 (MQ=255)
                                                                                                        tCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGtt    <  1:3593731/151‑1 (MQ=255)
                                                                                                        tCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGtt    <  1:3594198/151‑1 (MQ=255)
                                                                                                         cAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTc   >  1:2111798/1‑151 (MQ=255)
                                                                                                          agaaCCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCt  <  2:1820984/151‑1 (MQ=255)
                                                                                                          agaaCCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCAGCGCTGCGAGGAGGCGAATAATACGCGCGTTGAAATCAGAGTCAACACCTTGTTCt  >  1:4108660/1‑151 (MQ=255)
                                                                                                             |                                                                                                                                                   
GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCT  >  CP009974/775347‑775603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑