Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 994,351 C→T T400I (ACC→ATC)  RPPX_04355 → RNA polymerase factor sigma‑54

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974994,3510CT99.1% 864.3 / ‑5.1 231T400I (ACC→ATC) RPPX_04355RNA polymerase factor sigma‑54
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (102/126);  total (104/127)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GAGCCTGCAGAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGACCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGAAACTGGTTG  >  CP009974/994205‑994497
                                                                                                                                                  |                                                                                                                                                  
gagCCTGCAGAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCAtt                                                                                                                                                >  1:934823/1‑151 (MQ=255)
 agCCTGCAGAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCAttt                                                                                                                                               >  2:1045291/1‑151 (MQ=255)
   ccTGCAGAGCCGTAACGAAACCCGGATGAAGGTTGCAACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAGGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTcg                                                                                                                                             <  1:250471/151‑1 (MQ=255)
    gtggagggCCGTAACGAATGCGGGGGGAAGGTTGCCACGGAGGTCGTCGGGCACGAACGCGGCTTCCGCGACCGCGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTcgc                                                                                                                                            <  2:5228050/144‑1 (MQ=255)
    cTGCAGAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTcgc                                                                                                                                            >  2:4319338/1‑151 (MQ=255)
     tGCAGAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTcgcg                                                                                                                                           >  1:1022316/1‑151 (MQ=255)
            ccGGAACGAAACCCTGATGGAGGGTGCCCCGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTAcc                                                                                                                                    <  2:2369499/151‑1 (MQ=255)
              gTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCAc                                                                                                                                  >  2:4072834/1‑151 (MQ=255)
                 aCGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCa                                                                                                                               <  2:4822878/151‑1 (MQ=255)
                   gAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGa                                                                                                                             <  1:3846207/151‑1 (MQ=255)
                   gAAACCCTGATGAAGGTTGCCACGCAGAACGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGGTGGTACTGCACGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTGCGCGGGTTACCACGCAGa                                                                                                                             >  1:4214383/1‑151 (MQ=255)
                    aaaCCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGaa                                                                                                                            <  2:838459/151‑1 (MQ=255)
                      aCCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAAt                                                                                                                          >  2:794259/1‑151 (MQ=255)
                      aCCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAAt                                                                                                                          >  1:547638/1‑151 (MQ=255)
                          tgatgaAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACAt                                                                                                                      <  2:2133703/151‑1 (MQ=255)
                             tgaAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGca                                                                                                                   >  2:3564643/1‑151 (MQ=255)
                                aGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCacac                                                                                                                <  2:2461120/151‑1 (MQ=255)
                                 ggTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACAcc                                                                                                               <  2:1797355/151‑1 (MQ=255)
                                  gTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACAccc                                                                                                              <  1:3239166/151‑1 (MQ=255)
                                     gCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACGACGCAGAAATACATGCACACCAcgc                                                                                                           >  2:3454666/1‑151 (MQ=255)
                                     gCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCcgc                                                                                                           <  1:2871697/151‑1 (MQ=255)
                                           cAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCa                                                                                                     >  1:988678/1‑151 (MQ=255)
                                            aGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCAt                                                                                                    >  2:2861441/1‑151 (MQ=255)
                                             gATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATc                                                                                                   >  2:1939452/1‑151 (MQ=255)
                                                cgtcgAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTAc                                                                                                <  2:2100275/151‑1 (MQ=255)
                                                 gtcgAGCAGCAGCGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATCCACACCCCGCGTGGCATCTACg                                                                                               <  2:4658976/151‑1 (MQ=255)
                                                 gtcgAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACg                                                                                               >  2:3362859/1‑151 (MQ=255)
                                                     aGCGCCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACt                                                                                           <  1:1333270/151‑1 (MQ=255)
                                                      gCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTg                                                                                          <  1:1212784/151‑1 (MQ=255)
                                                           aaCGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATa                                                                                     >  1:2235544/1‑151 (MQ=255)
                                                              gcgGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACtt                                                                                  >  1:392172/1‑151 (MQ=255)
                                                              gcgGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACtt                                                                                  <  1:4009591/151‑1 (MQ=255)
                                                              gcgGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACtt                                                                                  >  1:1972732/1‑151 (MQ=255)
                                                              gcgGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACtt                                                                                  >  2:4262066/1‑151 (MQ=255)
                                                               cgGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACttt                                                                                 >  2:1432878/1‑151 (MQ=255)
                                                                ggCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACtttt                                                                                <  1:4073861/151‑1 (MQ=255)
                                                                 gCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACttttt                                                                               >  1:4932923/1‑151 (MQ=255)
                                                                  cTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTtc                                                                              <  2:4910791/151‑1 (MQ=255)
                                                                  cTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTtc                                                                              <  2:495120/151‑1 (MQ=255)
                                                                  cTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTtc                                                                              >  1:4317704/1‑151 (MQ=255)
                                                                    tCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTtctc                                                                            <  1:383119/151‑1 (MQ=255)
                                                                     ccTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTcc                                                                           >  1:4488223/1‑151 (MQ=255)
                                                                      cTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCa                                                                          >  1:1457055/1‑151 (MQ=255)
                                                                      cTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCa                                                                          >  2:1992151/1‑151 (MQ=255)
                                                                      cTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCa                                                                          <  2:1602609/151‑1 (MQ=255)
                                                                      cTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATGTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCa                                                                          <  2:3072082/151‑1 (MQ=255)
                                                                       tCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAg                                                                         >  1:4395581/1‑151 (MQ=255)
                                                                       tCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAg                                                                         <  2:3064166/151‑1 (MQ=255)
                                                                       gcGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAg                                                                         <  2:4763231/150‑1 (MQ=255)
                                                                        cGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGc                                                                        <  2:2167691/151‑1 (MQ=255)
                                                                         gACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGcc                                                                       >  2:4296545/1‑151 (MQ=255)
                                                                           ccACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCAt                                                                     <  2:3986895/151‑1 (MQ=255)
                                                                            cACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATg                                                                    <  1:1304265/151‑1 (MQ=255)
                                                                             aCGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGt                                                                   <  2:2494541/151‑1 (MQ=255)
                                                                              cGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTc                                                                  <  2:2144007/151‑1 (MQ=255)
                                                                               ggCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCa                                                                 >  2:752274/1‑151 (MQ=255)
                                                                                 cgacgaAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGc                                                               >  1:2449407/1‑151 (MQ=255)
                                                                                  gacgaAGCGATGAAGCCTTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCa                                                              <  1:1720028/151‑1 (MQ=255)
                                                                                  gacgaAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCa                                                              <  1:1825068/151‑1 (MQ=255)
                                                                                   acgaAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCAc                                                             >  1:3515763/1‑151 (MQ=255)
                                                                                      aaGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTc                                                          >  2:535054/1‑151 (MQ=255)
                                                                                      aaGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTc                                                          <  1:2522721/151‑1 (MQ=255)
                                                                                        gCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCg                                                        <  2:1297565/151‑1 (MQ=255)
                                                                                         cGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGa                                                       >  2:161351/1‑151 (MQ=255)
                                                                                          gATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAg                                                      <  1:4585990/151‑1 (MQ=255)
                                                                                            tGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAGTACTTTTTCTCCAGCCATGTCAGCACCTCCGAggg                                                    >  2:3133098/1‑151 (MQ=255)
                                                                                              aaGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCCTGTCAGCACCTCCGAGGGCg                                                  >  2:2836597/1‑151 (MQ=255)
                                                                                               aGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGGTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCgg                                                 >  1:4757517/1‑151 (MQ=255)
                                                                                                  cGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGgaga                                              <  1:4051154/151‑1 (MQ=255)
                                                                                                    ttGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAAt                                            >  1:142285/1‑151 (MQ=255)
                                                                                                    ttGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAAt                                            <  1:2756011/151‑1 (MQ=255)
                                                                                                    ttGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAAt                                            <  2:1209497/151‑1 (MQ=255)
                                                                                                          cTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGt                                      <  1:4149437/151‑1 (MQ=255)
                                                                                                           tGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTc                                     <  2:3852648/151‑1 (MQ=255)
                                                                                                                gACATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACgg                                <  1:2961359/151‑1 (MQ=255)
                                                                                                                 aCATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCCCGGc                               >  2:3914660/1‑151 (MQ=255)
                                                                                                                  cATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCg                              <  1:729380/151‑1 (MQ=255)
                                                                                                                  cATTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCg                              >  1:5105888/1‑151 (MQ=255)
                                                                                                                   aTTGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGa                             >  2:1212652/1‑151 (MQ=255)
                                                                                                                    ttGCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGAt                            <  2:3268906/151‑1 (MQ=255)
                                                                                                                     tGCAGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCAGAAAATACTCGACCAcgacgaac                           <  2:2007024/151‑3 (MQ=255)
                                                                                                                      gCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATcc                          <  1:5046950/151‑1 (MQ=255)
                                                                                                                      gCCGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATcc                          >  1:3320291/1‑151 (MQ=255)
                                                                                                                       gcGAAGCCTTAGGCATGCAAGAATCGATCATTTCGCGGTTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCcg                         <  1:43634/150‑1 (MQ=255)
                                                                                                                        cGAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGACGGCGGAGAATGCTCGTCCACGGCGATCcgc                        >  2:4481929/1‑151 (MQ=255)
                                                                                                                         gAAGCCGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCcgcg                       >  1:4679386/1‑151 (MQ=255)
                                                                                                                             ccGTAGTCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAATTACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGat                   <  2:1095197/151‑1 (MQ=255)
                                                                                                                             ccGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGat                   >  2:270755/1‑151 (MQ=255)
                                                                                                                              cGTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGatc                  >  1:152868/1‑151 (MQ=255)
                                                                                                                               gTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGatca                 <  1:3113203/151‑1 (MQ=255)
                                                                                                                               gTAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGatca                 <  1:249234/151‑1 (MQ=255)
                                                                                                                                tAGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGatcat                >  2:644963/1‑151 (MQ=255)
                                                                                                                                 aGGCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGatcatc               >  2:1875551/1‑151 (MQ=255)
                                                                                                                                   gCATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCaa             <  2:3193074/151‑1 (MQ=255)
                                                                                                                                    cATGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAg            <  1:3362859/151‑1 (MQ=255)
                                                                                                                                     aTGCACGAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGa           >  2:1067522/1‑151 (MQ=255)
                                                                                                                                           gAATCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTCGGAAGTGCGATGCTTTTTCTCCGGGCGTGGCAGCACGTCGTCGCGGGGAGAATGAACGACAACGGCGATCCGCGCGATCATCAAGAAACTgg     >  2:2924098/1‑151 (MQ=255)
                                                                                                                                             aTCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGAAACTGGtt   >  1:4266250/1‑151 (MQ=255)
                                                                                                                                              tCGATCATTTCGCGGGTTCCCACGTAGAAATACATGGACACGCCGAGTGGGATCTACGACCTGAAATACTTTTTCTCCAGCCATGTCAGCGCCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGAAACTGGTTg  <  2:1731202/151‑1 (MQ=255)
                                                                                                                                              tCGATCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGCAACTGGTTg  >  2:3525236/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAGCCTGCAGAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAACGCGGCTTCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGACCATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGTCAGCACCTCCGAGGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGAAACTGGTTG  >  CP009974/994205‑994497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑