Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,155,982 C→T T23S (ACC→TCT)  RPPX_05120 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,155,9820CT89.8% 983.6 / 67.4 325T23S (ACC→TCT) RPPX_05120hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (12/21);  new base T (146/146);  total (158/167)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

Only 100 of 330 total aligned reads displayed.

GCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCCGGGGCGAGATCGATCAGGAAGGTGCCGTTGGCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCAGCGTCACGCTGTTCCCCGCCCAGGCCAATGGTGAAGCACTCGATGTGCGTCTGGTGGATGCCGCCGGCACCGGCTTC  >  CP009974/1155838‑1156129
                                                                                                                                                |                                                                                                                                                   
cgatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTg                                                                                                                                               >  2:4663050‑M1/140‑151 (MQ=255)
 gatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGc                                                                                                                                              <  2:3461361‑M1/13‑1 (MQ=255)
  atgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCa                                                                                                                                             >  2:1365056‑M1/138‑151 (MQ=255)
   tgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAg                                                                                                                                            >  2:3992692‑M1/137‑151 (MQ=255)
    gcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAgg                                                                                                                                           <  2:3000537‑M1/16‑1 (MQ=255)
    gcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAgg                                                                                                                                           <  1:586786‑M1/16‑1 (MQ=255)
     cggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCCTGCaggg                                                                                                                                          <  2:217063‑M1/17‑2 (MQ=255)
        caggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCg                                                                                                                                       <  1:2214191‑M1/20‑1 (MQ=255)
        caggcaacgcctccaccgccaataaccgtaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCg                                                                                                                                       >  2:2102033‑M1/132‑151 (MQ=255)
         ggcgcacgcctccgccgcccataaccatgcggcggcggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGt                                                                                                                                      >  2:3146546‑M1/131‑151 (MQ=255)
         aggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGt                                                                                                                                      <  1:1967193‑M1/21‑1 (MQ=255)
           gcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccactctggtgatcggcgctgccggcagccagcccagcggtcgaggcgaagccGGCCCTACCGTGCAGGTGCGTGa                                                                                                                                    <  1:971364‑M1/23‑1 (MQ=255)
           gcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGa                                                                                                                                    <  1:3302568‑M1/23‑1 (MQ=255)
            caacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAc                                                                                                                                   <  1:4104251‑M1/24‑1 (MQ=255)
            caacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAc                                                                                                                                   >  2:4552256‑M1/128‑151 (MQ=255)
            caacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAc                                                                                                                                   >  2:3888953‑M1/128‑151 (MQ=255)
             aacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcg                                                                                                                                  <  2:3628455‑M1/25‑1 (MQ=255)
             aacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcg                                                                                                                                  >  2:3238937‑M1/127‑151 (MQ=255)
             aacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcg                                                                                                                                  >  2:708867‑M1/127‑151 (MQ=255)
                gcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccacctgaggccccgaccgatctggtcatcggcgctgccggcaccccgcccagccgtcgacgcgaagccGGCTCTCCCGTGCAGGTCCCTGACGcgg                                                                                                                               <  2:3847095‑M1/28‑1 (MQ=255)
                  ctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACgcggca                                                                                                                             <  2:4913663‑M1/30‑2 (MQ=255)
                  ctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCGCTACCGTTCAGGTGcgcgacgtcgca                                                                                                                             <  2:2049801‑M1/30‑6 (MQ=255)
                   tccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGcggccg                                                                                                                            >  2:5031370‑M1/121‑151 (MQ=255)
                    ccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGcggccgg                                                                                                                           <  2:1624134‑M1/32‑1 (MQ=255)
                      accgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTggcaggca                                                                                                                         <  1:124154‑M1/34‑1 (MQ=255)
                        cgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAAc                                                                                                                       >  1:212443‑M1/116‑151 (MQ=255)
                          ccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACAt                                                                                                                     <  1:3805189‑M1/38‑1 (MQ=255)
                           caataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgcagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATc                                                                                                                    <  1:1767084‑M1/39‑1 (MQ=255)
                           caataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATc                                                                                                                    >  1:3421897‑M1/113‑151 (MQ=255)
                             ataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCt                                                                                                                  <  1:5087908‑M1/41‑1 (MQ=255)
                              taaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTc                                                                                                                 >  2:4194281‑M1/110‑151 (MQ=255)
                              taaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTc                                                                                                                 >  2:3764366‑M1/110‑151 (MQ=255)
                              ggcccttgccgcgccggacagggacggcggcgacacaacgccaccggaggccccgaccaatgtggggatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTc                                                                                                                 >  2:4046642‑M1/110‑151 (MQ=255)
                                   ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGAc                                                                                                            >  1:1515482‑M1/105‑151 (MQ=255)
                                   ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGAc                                                                                                            <  2:3415772‑M1/47‑1 (MQ=255)
                                    taccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGAcc                                                                                                           >  2:1538194‑M1/104‑151 (MQ=255)
                                     accgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCg                                                                                                          >  1:4282973‑M1/103‑151 (MQ=255)
                                        gcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCa                                                                                                       >  2:2214191‑M1/100‑151 (MQ=255)
                                        gcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCa                                                                                                       >  2:2472558‑M1/100‑151 (MQ=255)
                                         cgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCAc                                                                                                      >  2:545516‑M1/99‑151 (MQ=255)
                                          gccggacttcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCAcc                                                                                                     >  1:1330006‑M1/98‑151 (MQ=255)
                                          gccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCAcc                                                                                                     >  2:1716800‑M1/98‑151 (MQ=255)
                                          gccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCAcc                                                                                                     <  2:2274776‑M1/54‑1 (MQ=255)
                                           ccggacagcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCg                                                                                                    >  2:442089‑M1/97‑151 (MQ=255)
                                            cggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGt                                                                                                   <  2:2843680‑M1/56‑1 (MQ=255)
                                            cggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGt                                                                                                   >  1:351710‑M1/96‑151 (MQ=255)
                                            cggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGt                                                                                                   <  1:4360693‑M1/56‑1 (MQ=255)
                                             ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTc                                                                                                  <  2:928922‑M1/57‑1 (MQ=255)
                                             ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTc                                                                                                  >  1:1799138‑M1/95‑151 (MQ=255)
                                             ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTc                                                                                                  >  2:3579200‑M1/95‑151 (MQ=255)
                                              gacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGCGACGCGGCCGGCGACCGCCGCGCGACCGGCaccggcg                                                                                                 <  2:4837465‑M1/58‑4 (MQ=255)
                                               acatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCCGGCAACATCCTCGCGACCGGCACCGTcgg                                                                                                <  2:2156986‑M1/59‑2 (MQ=255)
                                               acatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGc                                                                                                <  1:1641721‑M1/59‑1 (MQ=255)
                                               acatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGc                                                                                                >  2:1892568‑M1/93‑151 (MQ=255)
                                                catcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCgcc                                                                                               <  2:4724042‑M1/60‑2 (MQ=255)
                                                catcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCt                                                                                               >  1:1915745‑M1/92‑151 (MQ=255)
                                                catcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccAGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCt                                                                                               >  1:4728541‑M1/92‑151 (MQ=255)
                                                  tcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGc                                                                                             >  1:2287253‑M1/90‑151 (MQ=255)
                                                   cgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCa                                                                                            >  2:185992‑M1/89‑151 (MQ=255)
                                                    gacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAg                                                                                           >  1:929878‑M1/88‑151 (MQ=255)
                                                    gacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAg                                                                                           <  2:3328645‑M1/64‑1 (MQ=255)
                                                     acggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGa                                                                                          >  2:164308‑M1/87‑151 (MQ=255)
                                                      cggcggcgacacaacgccaccggagtccccgaccaatctggtgagcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGAt                                                                                         >  2:4063778‑M1/86‑151 (MQ=255)
                                                       ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAAATg                                                                                        <  1:4861328‑M1/67‑1 (MQ=255)
                                                        gcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATgg                                                                                       >  1:4659150‑M1/84‑151 (MQ=255)
                                                         gggcgacgcgacgccaccggaggccccgaccaatctggtgatcggcgcggccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGc                                                                                      >  2:4075995‑M1/83‑151 (MQ=255)
                                                          ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                                                                                     <  2:1126998‑M1/70‑1 (MQ=255)
                                                          ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                                                                                     <  1:2115865‑M1/70‑1 (MQ=255)
                                                          ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                                                                                     <  1:101404‑M1/70‑1 (MQ=255)
                                                          ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                                                                                     >  1:1188569‑M1/82‑151 (MQ=255)
                                                            cgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCCCCGTCGCTGCCGATGGCAcc                                                                                   <  2:3049170‑M1/72‑1 (MQ=255)
                                                            cgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCAcc                                                                                   <  1:731404‑M1/72‑1 (MQ=255)
                                                             gacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCt                                                                                  >  1:2973597‑M1/79‑151 (MQ=255)
                                                             gacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCt                                                                                  <  1:5140213‑M1/73‑1 (MQ=255)
                                                               cacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTc                                                                                >  2:2421291‑M1/77‑151 (MQ=255)
                                                                acaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCa                                                                               <  1:3085461‑M1/76‑1 (MQ=255)
                                                                 caacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTcac                                                                              >  1:1875689‑M1/75‑150 (MQ=255)
                                                                 caacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTcac                                                                              >  1:2527751‑M1/75‑150 (MQ=255)
                                                                  aacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTtcacc                                                                             >  2:627762‑M1/74‑149 (MQ=255)
                                                                   acgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCttcacca                                                                            <  1:4486553‑M1/79‑4 (MQ=255)
                                                                    cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                                                                           >  1:316052‑M1/72‑147 (MQ=255)
                                                                    cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                                                                           >  1:2465231‑M1/72‑147 (MQ=255)
                                                                    cgccaacggaggcacctaccaagctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                                                                           >  2:4597737‑M1/72‑147 (MQ=255)
                                                                       caccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGcaccttcaccatcac                                                                        >  2:2661651‑M1/69‑144 (MQ=255)
                                                                       caccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGcaccttcaccatcac                                                                        <  1:4525337‑M1/83‑8 (MQ=255)
                                                                              agcACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggac                                                                 <  2:2616917‑M2/150‑15 (MQ=255)
                                                                                cACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccc                                                               <  2:182665‑M2/151‑17 (MQ=255)
                                                                                 aCCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccct                                                              >  2:128057‑M2/1‑134 (MQ=255)
                                                                                  ccGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctg                                                             >  1:4044396‑M2/1‑133 (MQ=255)
                                                                                  ccGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctg                                                             >  1:418207‑M2/1‑133 (MQ=255)
                                                                                   cGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgc                                                            >  2:869152‑M2/1‑132 (MQ=255)
                                                                                    gACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggcccctgcc                                                           <  2:2526670‑M2/151‑21 (MQ=255)
                                                                                      ccAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgt                                                         >  2:4512073‑M2/1‑129 (MQ=255)
                                                                                      ccAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgt                                                         <  1:2671712‑M2/151‑23 (MQ=255)
                                                                                       cAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcctcgccctggcccctgccgtg                                                        <  2:4298838‑M2/151‑24 (MQ=255)
                                                                                       cAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtg                                                        >  1:2821646‑M2/1‑128 (MQ=255)
                                                                                       cAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtg                                                        <  2:894182‑M2/151‑24 (MQ=255)
                                                                                        aaTCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtga                                                       >  2:2792601‑M2/1‑127 (MQ=255)
                                                                                        aaTCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtga                                                       <  1:3565275‑M2/151‑25 (MQ=255)
                                                                                                                                             caccacCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgaatgacggcagcaccttgcaggtcaccctcgccgatgccgccggcaacgtatcc  >  1:1676071‑M2/1‑74 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCCGGGGCGAGATCGATCAGGAAGGTGCCGTTGGCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCAGCGTCACGCTGTTCCCCGCCCAGGCCAATGGTGAAGCACTCGATGTGCGTCTGGTGGATGCCGCCGGCACCGGCTTC  >  CP009974/1155838‑1156129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑